Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A15 F64 I1 R1 4 152.9 5325952 98.7% 5256714 135.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 4,178,501 G→C R1075P (CGT→CCT)  rpoC → RNA polymerase, beta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,178,5010GC94.3% 642.0 / 28.2 192R1075P (CGT→CCT) rpoCRNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base G (3/8);  new base C (74/107);  total (77/115)
Fisher's exact test for biased strand distribution p-value = 5.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.96e-01

Only 100 of 193 total aligned reads displayed.

ACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACC  >  CP009273/4178362‑4178637
                                                                                                                                           |                                                                                                                                        
aCCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCg                                                                                                                                          >  2:1831705/1‑140 (MQ=255)
    aaGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCg                                                                                                                                      >  2:2468334/1‑140 (MQ=255)
    aaGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCg                                                                                                                                      >  2:521541/1‑140 (MQ=255)
    aaGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCg                                                                                                                                      >  1:1725963/1‑140 (MQ=255)
         aGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCATAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACt                                                                                                                                 >  1:2424046/1‑140 (MQ=255)
         aGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACt                                                                                                                                 >  2:1409730/1‑140 (MQ=255)
         aGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACt                                                                                                                                 >  1:2358661/1‑140 (MQ=255)
         aGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCCTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACt                                                                                                                                 >  2:714737/1‑140 (MQ=255)
                  gTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGAGTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGt                                                                                                                        <  2:196629/140‑1 (MQ=255)
                       cTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATg                                                                                                                   >  1:282934/1‑140 (MQ=255)
                        tttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGc                                                                                                                  >  1:1082668/1‑140 (MQ=255)
                        tttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGc                                                                                                                  >  1:1380538/1‑140 (MQ=255)
                        tttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTGCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGc                                                                                                                  >  2:1016960/1‑140 (MQ=255)
                         ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCt                                                                                                                 <  1:1831705/140‑1 (MQ=255)
                         ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATACt                                                                                                                 <  2:2162490/140‑1 (MQ=255)
                         ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCt                                                                                                                 <  2:2314548/140‑1 (MQ=255)
                         ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCt                                                                                                                 <  1:265485/140‑1 (MQ=255)
                         ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCt                                                                                                                 <  2:2373239/140‑1 (MQ=255)
                         ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCt                                                                                                                 <  1:960872/140‑1 (MQ=255)
                         ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCt                                                                                                                 <  2:2072425/140‑1 (MQ=255)
                         ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCt                                                                                                                 <  2:215521/140‑1 (MQ=255)
                         ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCt                                                                                                                 <  2:1082668/140‑1 (MQ=255)
                         ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCt                                                                                                                 <  2:1216105/140‑1 (MQ=255)
                         ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCt                                                                                                                 <  2:2571231/140‑1 (MQ=255)
                         ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCt                                                                                                                 <  1:2520100/140‑1 (MQ=255)
                         ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCt                                                                                                                 <  2:1784323/140‑1 (MQ=255)
                         ttACTGACATCATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTTGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCt                                                                                                                 <  1:1544814/140‑1 (MQ=255)
                         ttACTGACAGGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCt                                                                                                                 <  1:2103954/140‑1 (MQ=255)
                          tACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTc                                                                                                                >  2:1037159/1‑140 (MQ=255)
                          tACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATACCGCATAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCTTTGATGCTc                                                                                                                >  2:1345830/1‑140 (MQ=255)
                                cATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTg                                                                                                                      <  1:797893/128‑1 (MQ=255)
                                   gATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacg                                                                                                       <  1:678254/140‑1 (MQ=255)
                                   gATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacg                                                                                                       <  1:2629289/140‑1 (MQ=255)
                                        aCGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGtt                                                                                                  <  1:263602/140‑1 (MQ=255)
                                         cGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTc                                                                                                 >  1:174569/1‑140 (MQ=255)
                                         cGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACGGAAAATCCTTGATGCTCAGGGTAACGACGTTc                                                                                                 >  1:2629097/1‑140 (MQ=255)
                                         cGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCAGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACGGCAGGTGGTAAAGATCTGCCTCCGGCACTCAAAATCGTTGAGGCTCAGGGTAACGACGTTc                                                                                                 >  2:1557719/1‑140 (MQ=255)
                                           gCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCt                                                                                                                 <  1:2295090/122‑1 (MQ=255)
                                           gCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTg                                                                                               <  2:359634/140‑1 (MQ=255)
                                                aCCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCt                                                                                                                 <  2:1716300/117‑1 (MQ=255)
                                                aCCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCt                                                                                                                 >  1:1716300/1‑117 (MQ=255)
                                                aCCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATccc                                                                                          <  1:2468334/140‑1 (MQ=255)
                                                    ttACGCGTCAGACCGACGAACTGACCGTTCTGTCTTCGCTGGTGGTTCTTGATTCCTCAGAACGTACCTCATGTGTTAAAGATCTGACTCCGTCACTGAAAATAGTTGATGCTCATTGTAACGACGTTCTGATccaatgt                                                                                      <  2:763137/140‑6 (MQ=255)
                                                    ttACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGt                                                                                      <  1:677155/140‑1 (MQ=255)
                                                    ttACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGt                                                                                      <  2:2412142/140‑1 (MQ=255)
                                                    ttACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGt                                                                                      >  1:1687554/1‑140 (MQ=255)
                                                    ttACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGt                                                                                      <  2:314804/140‑1 (MQ=255)
                                                    ttACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCGGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGt                                                                                      <  1:1749648/140‑1 (MQ=255)
                                                     tACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGACCGTCCCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAACATCGTTGATGCTGCGGGTCACGACGTTCTGATCCCAGGTa                                                                                     >  2:1644833/1‑140 (MQ=255)
                                                         cgTGAGACCGACTAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGa                                                                                 <  1:2062992/140‑1 (MQ=255)
                                                         cgTCAGACCGACGCACTGCCCGGTCTGTGTTCGCTGGTGGTTCTGGATTGCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGa                                                                                 <  2:1533455/140‑1 (MQ=255)
                                                            cAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGatat                                                                              >  1:375451/1‑140 (MQ=255)
                                                            cAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGatat                                                                              >  2:2511339/1‑140 (MQ=255)
                                                            cAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGatat                                                                              >  2:264257/1‑140 (MQ=255)
                                                              gACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGc                                                                            <  1:939037/140‑1 (MQ=255)
                                                              gACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGc                                                                            <  2:1165762/140‑1 (MQ=255)
                                                              gACAGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGc                                                                            <  2:2278490/140‑1 (MQ=255)
                                                                 cgacgaACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAg                                                                         <  1:1019453/140‑1 (MQ=255)
                                                                 cgacgaACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAg                                                                         <  1:476933/140‑1 (MQ=255)
                                                                  gacgaACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAgc                                                                        <  1:1839077/140‑1 (MQ=255)
                                                                   acgaACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAgcg                                                                       >  1:545277/1‑140 (MQ=255)
                                                                    cgaACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAgcgc                                                                      >  1:1149428/1‑140 (MQ=255)
                                                                    cgaACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAgcgc                                                                      >  1:836142/1‑140 (MQ=255)
                                                                                       tCGCTGGTGGTTCTGGAGTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTaa                                                   <  1:1488819/140‑1 (MQ=255)
                                                                                              tggtaatGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCACCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGAtt                                            >  2:1692374/7‑140 (MQ=255)
                                                                                              tggtTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacg                                                                                                       <  2:246014/81‑1 (MQ=255)
                                                                                              tggtTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacg                                                                                                       >  1:246014/1‑81 (MQ=255)
                                                                                              tggtTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGAtt                                            >  2:951659/1‑140 (MQ=255)
                                                                                              tggtTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGAtt                                            >  2:78335/1‑140 (MQ=255)
                                                                                              tggtTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGAtt                                            >  1:2492063/1‑140 (MQ=255)
                                                                                                 ttCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGtt                                         <  2:174569/140‑1 (MQ=255)
                                                                                                 ttCTGGATTACGCAGAACGTACCGAAGGTGGTAAAGATCTGCCTCCCGCACAGCAAATCGTTGATGCTCAGGGTAACTACGTTCTGATCCCAGGTACCGATATGCCAGAGCAGTACTTCCTGCCGGGTAAAGCGATTGtt                                         <  2:2619442/140‑1 (MQ=255)
                                                                                                  tCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTc                                        >  1:163343/1‑140 (MQ=255)
                                                                                                           cGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAg                               >  2:1524306/1‑140 (MQ=255)
                                                                                                           cGCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAg                               >  2:288980/1‑140 (MQ=255)
                                                                                                            gCAGAACGTACCGCAGGTTGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGGTCAGCTGGAAGa                              <  1:490186/140‑1 (MQ=255)
                                                                                                            gCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGa                              <  1:2170678/140‑1 (MQ=255)
                                                                                                            gCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGa                              <  1:183075/140‑1 (MQ=255)
                                                                                                            gCAGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGa                              <  2:346142/140‑1 (MQ=255)
                                                                                                              aGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATg                            <  1:2338056/140‑1 (MQ=255)
                                                                                                              aGAACGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATg                            <  1:636193/140‑1 (MQ=255)
                                                                                                                 aCGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCg                         <  2:1493165/140‑1 (MQ=255)
                                                                                                                 aCGTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCg                         <  2:489653/140‑1 (MQ=255)
                                                                                                                   gTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTa                       <  2:801436/140‑1 (MQ=255)
                                                                                                                   gTACCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTa                       <  2:2260047/140‑1 (MQ=255)
                                                                                                                     aCCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAg                                      <  1:1181728/123‑1 (MQ=255)
                                                                                                                     aCCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACa                     >  2:334099/1‑140 (MQ=255)
                                                                                                                     aCCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACa                     >  2:904662/1‑140 (MQ=255)
                                                                                                                     aCCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACa                     >  2:1172537/1‑140 (MQ=255)
                                                                                                                     aCCGCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGAGTGTTCAGCTGGAAGATGGCGTACa                     >  2:192232/1‑140 (MQ=255)
                                                                                                                        gCAGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGAt                  <  2:2117847/140‑1 (MQ=255)
                                                                                                                          aGGTGGTAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCa                >  2:396742/1‑140 (MQ=255)
                                                                                                                            gtggtAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGc              >  1:2090551/1‑140 (MQ=255)
                                                                                                                             tggtAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGct             <  1:1345830/140‑1 (MQ=255)
                                                                                                                             tggtAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGct             <  1:1134784/140‑1 (MQ=255)
                                                                                                                             tggtAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGct             <  1:2381971/140‑1 (MQ=255)
                                                                                                                              ggtAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGctc            <  2:537732/140‑1 (MQ=255)
                                                                                                                              ggtAAAGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGctc            <  2:2026597/140‑1 (MQ=255)
                                                                                                                                   aGATCTGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTg       <  1:1016960/140‑1 (MQ=255)
                                                                                                                                        tGCCTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACAcc  >  2:65626/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                        
ACCGAAGTAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACC  >  CP009273/4178362‑4178637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A