Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A13 F64 I0 R1 334 49.4 1968632 94.1% 1852482 134.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,165,449 C→T 30.4% noncoding (50/85 nt) tyrU → tRNA‑Tyr

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,165,4490CT30.4% 119.7 / 69.4 79noncoding (50/85 nt)tyrUtRNA‑Tyr
Reads supporting (aligned to +/- strand):  ref base C (31/24);  new base T (6/18);  total (37/42)
Fisher's exact test for biased strand distribution p-value = 1.40e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01

TTGATGCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTGCAGAATGAAAATCAGGTAGCC  >  CP009273/4165311‑4165588
                                                                                                                                          |                                                                                                                                           
ttGATGCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTcaca                                                                                                                                            >  1:474934/1‑140 (MQ=255)
    tGCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACt                                                                                                                                        >  2:923118/1‑140 (MQ=255)
    tGCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACt                                                                                                                                        >  2:737539/1‑140 (MQ=255)
    tGCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACt                                                                                                                                        >  2:75431/1‑140 (MQ=255)
    tGCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACt                                                                                                                                        >  2:75674/1‑140 (MQ=255)
    tGCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACt                                                                                                                                        >  2:799579/1‑140 (MQ=255)
    tGCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACt                                                                                                                                        >  2:132257/1‑140 (MQ=255)
    tGCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACt                                                                                                                                        >  2:421728/1‑140 (MQ=255)
        gACTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGa                                                                                                                                    <  2:133204/140‑1 (MQ=255)
        gACTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGAGTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGa                                                                                                                                    <  2:622919/140‑1 (MQ=255)
        gACTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGAGTCCGGGAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGa                                                                                                                                    <  2:628004/140‑1 (MQ=255)
         aattAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                                   <  2:682301/138‑1 (MQ=255)
         aCTTAGCTTAGTAGGTAGAGCAAGTGACTTGTAATCAGTAGGTCACCAGTTCGATTGCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGTGCAGACTGTAAATCTGCCGTGATCGACTTCGaa                                                                                                                                   <  2:628425/140‑1 (MQ=37)
         aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGATCGGCAAAAGGGAGCTGACTGTAAATCTGATGTCATCGACTTCGat                                                                                                                                   <  2:916747/140‑2 (MQ=255)
         aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCATACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                                   <  2:665752/140‑1 (MQ=255)
         aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                                   <  2:522523/140‑1 (MQ=255)
         aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                                   <  2:408184/140‑1 (MQ=255)
         aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                                   <  2:142438/140‑1 (MQ=255)
         aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                                   <  2:695989/140‑1 (MQ=255)
         aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                                   <  2:408174/140‑1 (MQ=255)
         aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                                   <  2:177797/140‑1 (MQ=255)
         aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                                   <  2:832992/140‑1 (MQ=255)
         aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                                   <  2:904425/140‑1 (MQ=255)
         aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                                   <  2:920199/140‑1 (MQ=255)
         aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                                   <  2:715928/140‑1 (MQ=255)
         aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                                   <  2:140136/140‑1 (MQ=255)
         aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                                   >  1:65550/1‑140 (MQ=255)
         aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                                   <  2:398743/140‑1 (MQ=255)
         aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                                   <  2:203833/140‑1 (MQ=255)
         aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGGTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                                   <  2:66170/140‑1 (MQ=255)
         aCTTACCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                                   <  2:291933/140‑1 (MQ=255)
         aCTGAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                                   <  2:799418/140‑1 (MQ=255)
                 cAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGaa                                                                                                                           >  1:359071/1‑140 (MQ=255)
                    taggtagAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGTGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATcc                                                                                                                        >  2:755844/1‑140 (MQ=255)
                    taggtagAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATcc                                                                                                                        >  1:884424/1‑140 (MQ=255)
                    taggtagAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATcc                                                                                                                        >  1:696402/1‑140 (MQ=255)
                    taggtagAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATcc                                                                                                                        >  1:64939/1‑140 (MQ=255)
                    taggtagAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATcc                                                                                                                        >  1:47300/1‑140 (MQ=255)
                    taggtagAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATcc                                                                                                                        >  1:153433/1‑140 (MQ=255)
                     aggtagAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                                   <  2:647613/128‑1 (MQ=255)
                     aggtagAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                                   >  1:647613/1‑128 (MQ=255)
                     aggtagAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCt                                                                                                                       >  1:32075/1‑140 (MQ=255)
                     aggtagAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                                   <  2:501360/128‑1 (MQ=255)
                     aggtagAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                                   >  1:501360/1‑128 (MQ=255)
                     aggtagAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCt                                                                                                                       >  1:813338/1‑140 (MQ=255)
                     aggtagAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCt                                                                                                                       >  1:404151/1‑140 (MQ=255)
                     aggtagAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCt                                                                                                                       >  1:378443/1‑140 (MQ=255)
                     aggtagAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCAGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCt                                                                                                                       >  1:355853/1‑140 (MQ=255)
                         agagCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTccc                                                                                                                   <  2:474934/140‑1 (MQ=255)
                           agCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTccccc                                                                                                                 >  2:799201/1‑140 (MQ=255)
                            gCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGGTCGAATCCTTCCCcca                                                                                                                >  2:181752/1‑140 (MQ=255)
                                cTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTaccacaccac                                                                                                            >  2:204553/1‑140 (MQ=255)
                                      tGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCccaccaccatcac                                                                                                      <  1:370439/140‑5 (MQ=255)
                                      tGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCccaccaccatcac                                                                                                      <  1:10068/140‑5 (MQ=255)
                                         aaTCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCccaccaccataattca                                                                                                   <  2:32075/140‑8 (MQ=255)
                                         aaTCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCgg                                                                                                   <  2:33905/140‑1 (MQ=255)
                                             aGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCAc                                                                                               >  2:249584/1‑140 (MQ=255)
                                              gTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCAcg                                                                                              >  1:264197/1‑140 (MQ=255)
                                               tAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCAcgc                                                                                             >  1:432746/1‑140 (MQ=255)
                                                aGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCAcgcg                                                                                            <  1:577973/140‑1 (MQ=255)
                                                  gTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGAt                                                                                          >  2:161457/1‑140 (MQ=255)
                                                                      aGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATaa                                                                      <  2:646008/140‑1 (MQ=255)
                                                                                 tCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTAcc                                                           >  1:267804/1‑140 (MQ=255)
                                                                                     gTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCt                                                       <  2:967012/140‑1 (MQ=255)
                                                                                         ggcggggTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGaa                                                   <  1:918501/137‑1 (MQ=255)
                                                                                         ggTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGaa                                                   <  1:686776/140‑1 (MQ=255)
                                                                                                ttCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAagag                                            >  2:933187/1‑140 (MQ=255)
                                                                                                ttCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAAGTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAagag                                            >  2:682775/1‑140 (MQ=255)
                                                                                                  cccGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAagagag                                          <  1:933187/140‑1 (MQ=255)
                                                                                                      aGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTtc                                      <  2:432746/140‑1 (MQ=255)
                                                                                                           ccAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGCAGCCTTCCCCCACCACGAATTTCCGCCACGCGATGGCGTAGCCCGAGAAGATATGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCg                                 <  2:967184/140‑1 (MQ=37)
                                                                                                           ccAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCg                                 <  2:404151/140‑1 (MQ=255)
                                                                                                           ccAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCg                                 <  2:355853/140‑1 (MQ=255)
                                                                                                                 ggAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTc                           <  1:755844/140‑1 (MQ=255)
                                                                                                                                  tGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTGCAGAATGAAAATc          >  2:397224/1‑140 (MQ=255)
                                                                                                                                  tGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTGCAGAATGAAAATc          >  2:23728/1‑140 (MQ=255)
                                                                                                                                    ccGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATa                                                                       >  1:592624/1‑77 (MQ=255)
                                                                                                                                    ccGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATa                                                                       <  2:592624/77‑1 (MQ=255)
                                                                                                                                    ccGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTGCAGAATGAAAATCAg        >  1:55693/1‑140 (MQ=255)
                                                                                                                                    ccGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTGCAGAATGAAAATCAg        >  1:617106/1‑140 (MQ=255)
                                                                                                                                          caGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTGCAGAATGAAAATCAGGTAGcc  <  1:305334/140‑1 (MQ=255)
                                                                                                                                          caGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTGCAGAATGAAAATCAGGTAGcc  <  1:816124/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                           
TTGATGCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTGCAGAATGAAAATCAGGTAGCC  >  CP009273/4165311‑4165588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A