Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F65 I0 R1	86	259.7	8019954	97.7%	7835495	136.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,177,586	T→C	100%	L770P (CTC→CCC)	rpoC →	RNA polymerase, beta prime subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,177,586	0	T	C	95.5%	594.1 / 13.3	183	L770P (CTC→CCC)	rpoC	RNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand): ref base T (4/4); new base C (80/94); total (85/98)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 183 total aligned reads displayed.

ATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGT  >  CP009273/4177450‑4177722

atgGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCa                                                                                                                                       >  2:1157431/1‑140 (MQ=255)

atgGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCa                                                                                                                                       >  2:799658/1‑140 (MQ=255)

atgGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCa                                                                                                                                       >  2:2146248/1‑140 (MQ=255)

atgGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCa                                                                                                                                       >  2:3736083/1‑140 (MQ=255)

 tgGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCa                                                                                                                                       >  1:2941876/1‑139 (MQ=255)

 tgGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAg                                                                                                                                      >  2:1741300/1‑140 (MQ=255)

 tgGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAg                                                                                                                                      >  2:1745777/1‑140 (MQ=255)

  ggCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCCGt                                                                                                                                     >  2:3312929/1‑140 (MQ=255)

  ggCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGt                                                                                                                                     >  1:173215/1‑140 (MQ=255)

  ggCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGt                                                                                                                                     >  2:3706342/1‑140 (MQ=255)

    ccGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCCATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTAc                                                                                                                                   >  2:3281489/1‑140 (MQ=255)

    ccGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTAc                                                                                                                                   >  2:461792/1‑140 (MQ=255)

    ccGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTAc                                                                                                                                   <  2:3381384/140‑1 (MQ=255)

      gACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCCGTAcct                                                                                                                                 <  1:1142281/140‑3 (MQ=255)

      gACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTAcct                                                                                                                                 <  1:1755255/140‑3 (MQ=255)

      gACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACtt                                                                                                                                 <  1:102457/140‑1 (MQ=255)

       aCTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTc                                                                                                                                >  1:2193833/1‑140 (MQ=255)

       aCTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTc                                                                                                                                >  1:1428677/1‑140 (MQ=255)

       aCTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTc                                                                                                                                <  2:3228181/140‑1 (MQ=255)

       aCTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTc                                                                                                                                >  1:1060837/1‑140 (MQ=255)

          ccGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGTGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCAtc                                                                                                                             <  1:2505038/140‑1 (MQ=255)

          ccGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCAtc                                                                                                                             <  1:1238807/140‑1 (MQ=255)

            ggTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCAtctc                                                                                                                           <  1:824711/140‑1 (MQ=255)

            ggTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCAtctc                                                                                                                           <  1:1176955/140‑1 (MQ=255)

            ggTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTGGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCAtctc                                                                                                                           <  1:228540/140‑1 (MQ=255)

                  cgTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTccaccca                                                                                                                     >  1:1850481/1‑140 (MQ=255)

                  cgTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTccaccca                                                                                                                     >  1:3610276/1‑140 (MQ=255)

                  cgTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTccaccca                                                                                                                     >  1:683259/1‑140 (MQ=255)

                  cgTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTccaccca                                                                                                                     >  2:3636652/1‑140 (MQ=255)

                    tGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACg                                                                                                                   <  2:1231438/140‑1 (MQ=255)

                      gTTCTGCGGCACAGATTCGTGAGCTTGATGGTATGCGTGCTCTCATGGCGAAGCCGGATGGCTCCATCCTCGAAACCCCAATCACCGCGAACTTCCGTGAAGCTCTGAACGTACCCCAGTAGTTCATCTCCACCCACGGt                                                                                                                 >  2:1297307/1‑140 (MQ=255)

                       ttCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTg                                                                                                                <  1:2122932/140‑1 (MQ=255)

                          tGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGACGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGGGCTc                                                                                                             >  1:2532511/1‑140 (MQ=255)

                          tGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGTTGCTc                                                                                                             >  1:1363024/1‑140 (MQ=255)

                           gCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACGCCAGTACTTCATCTCCACCCACGGTGCTCg                                                                                                            >  2:1381387/1‑140 (MQ=255)

                            cGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGt                                                                                                           >  2:891224/1‑140 (MQ=255)

                               cacaGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTaaa                                                                                                        >  1:3933882/1‑140 (MQ=255)

                                 caGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAgg                                                                                                      >  1:407543/1‑140 (MQ=255)

                                 caGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAgg                                                                                                      >  1:885588/1‑140 (MQ=255)

                                 caGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAgg                                                                                                      >  1:676414/1‑140 (MQ=255)

                                 caGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAgg                                                                                                      >  1:3204476/1‑140 (MQ=255)

                                  agagTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGt                                                                                                     <  1:3796615/140‑1 (MQ=255)

                                  aGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGCTGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGt                                                                                                     <  1:1040458/140‑1 (MQ=255)

                                  aGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGt                                                                                                     <  1:2372449/140‑1 (MQ=255)

                                  aGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGt                                                                                                     <  1:2611377/140‑1 (MQ=255)

                                  aGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGt                                                                                                     <  1:2855792/140‑1 (MQ=255)

                                  aGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGt                                                                                                     <  1:825069/140‑1 (MQ=255)

                                    aTTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCAGCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCt                                                                                                   >  1:3665374/1‑140 (MQ=255)

                                       cGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGc                                                                                                <  2:2484565/140‑1 (MQ=255)

                                       cGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGc                                                                                                <  2:1428677/140‑1 (MQ=255)

                                       cGTCAGCTTGCTGGTATGAGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGc                                                                                                <  1:2328103/140‑1 (MQ=255)

                                       cGTCAGCGTGCTGGTATGCGTGGTCTGATGGCGAATCCGGATGGCTCCATCATCGAAACGCCAATCCCCGCGAACTTCCGTGAAGGTCTGAAAGTACCCCAGTACTTCATCTCCAGCCACGGTGCTCGTAAAGGTCTGGc                                                                                                <  1:3498117/140‑1 (MQ=255)

                                         tccgCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGACGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCgg                                                                                              >  2:2132277/4‑140 (MQ=255)

                                          cAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGa                                                                                             <  2:1904253/140‑1 (MQ=255)

                                             cTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTAGTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATAc                                                                                          >  2:327371/1‑140 (MQ=255)

                                              ttGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCTTCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATAcc                                                                                         <  1:2216705/140‑1 (MQ=255)

                                              ttGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATAcc                                                                                         <  1:3874989/140‑1 (MQ=255)

                                              ttGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATAcc                                                                                         <  1:3693436/140‑1 (MQ=255)

                                                gCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGt                                                                                                                 <  2:1519102/114‑1 (MQ=255)

                                                gCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGCCTGGCGGATACCGc                                                                                       <  1:2159781/140‑1 (MQ=255)

                                                       tGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAATCGCCAATCACCGCGAACTTGCGTGTAGGTCTGAACGTCCCCCAGTACTTCATCTCCACCCACGGTGCTCGGAAAGGTCTGGCGGATACCGCACTGaca                                                                                >  2:2210263/1‑138 (MQ=255)

                                                       tGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGaaa                                                                                >  1:1680616/1‑140 (MQ=255)

                                                             gTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCg                                                                          <  2:242689/140‑1 (MQ=255)

                                                              tCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGa                                                                         >  2:3566515/1‑140 (MQ=255)

                                                              tCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGa                                                                         >  2:1798574/1‑140 (MQ=255)

                                                               cTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGaa                                                                        <  2:4005181/140‑1 (MQ=255)

                                                               cTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGaa                                                                        <  1:179549/140‑1 (MQ=255)

                                                                 gATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCCATCACCGCGAACTTCCGGGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGACCt                                                                      >  2:313115/1‑140 (MQ=255)

                                                                  aTGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTc                                                                     >  2:683343/1‑140 (MQ=255)

                                                                   tGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGCTCTTCCGTGAAGGTCTTAACGTACCCCACTACTTCATCTCCACCCAAGGTGCTCGTAAAGGTCTGGCGGATACCGCTCTGAAAACTGCGAAATcc                                                                    <  2:1288732/140‑1 (MQ=255)

                                                                        aaGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTa                                                               >  1:633169/1‑140 (MQ=255)

                                                                        aaGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTa                                                               >  1:2989423/1‑140 (MQ=255)

                                                                               aTGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACt                                                        >  1:1211667/1‑140 (MQ=255)

                                                                                tGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACtc                                                       >  2:555066/1‑140 (MQ=255)

                                                                                     ccATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTcgtcgt                                                  <  1:3717704/140‑1 (MQ=255)

                                                                                     ccATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTcgtcgt                                                  <  2:407543/140‑1 (MQ=255)

                                                                                     ccATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTcgtcgt                                                  <  2:2683864/140‑1 (MQ=255)

                                                                                      catcatCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCgtcgtc                                                 >  2:2928370/1‑140 (MQ=255)

                                                                                      catcatCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCgtcgtc                                                 >  2:2928490/1‑140 (MQ=255)

                                                                                         catcGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATCCCGCACTGAAAACTGAGAACTCCGGTTACCTGACTCGTCGTCTgg                                              >  2:1965774/1‑140 (MQ=255)

                                                                                            cGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTCCTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGCACTCCGGTTACCTGACTCGTCGTGTGGTTg                                           >  1:798904/1‑140 (MQ=255)

                                                                                            cGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTCCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTg                                           >  1:3402135/1‑140 (MQ=255)

                                                                                                 cGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTg                                      >  1:3389914/1‑140 (MQ=255)

                                                                                                    cAATCCCCGCGACCTTCCGTCAAGGTCTGACCGTACCCCAGGACTTCATCTCCACCCCCGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGgcg                                   <  2:1480313/140‑1 (MQ=255)

                                                                                                    cAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGgcg                                   <  1:1741300/140‑1 (MQ=255)

                                                                                                    cAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGgcg                                   <  1:262026/140‑1 (MQ=255)

                                                                                                    cAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGgcg                                   <  1:610183/140‑1 (MQ=255)

                                                                                                    cAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGgcg                                   <  1:3001327/140‑1 (MQ=255)

                                                                                                      aTCACCGCGAACTTGCGTGAAGGTCTGAACGTACCCCAGTACTTCATCGCCACCCACGGTGCTCGTAAAGCTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCa                                 <  2:367901/140‑1 (MQ=255)

                                                                                                      aTCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCa                                 <  2:3518033/140‑1 (MQ=255)

                                                                                                      aTCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCAAGGTGATAGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCa                                 <  2:1970158/140‑1 (MQ=255)

                                                                                                       tCACCGCGAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAg                                <  1:3422487/140‑1 (MQ=255)

                                                                                                           cgcgAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGAcc                            >  1:3554542/1‑140 (MQ=255)

                                                                                                             cgAACTTCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGc                                                                                                >  2:883113/1‑70 (MQ=255)

                                                                                                                   tCCGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGtt                    <  1:2146248/140‑1 (MQ=255)

                                                                                                                    ccGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTa                   <  2:2997088/140‑1 (MQ=255)

                                                                                                                     cGTGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTTGTGGTTAc                  >  1:153153/1‑140 (MQ=255)

                                                                                                                       tGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCg                >  1:2422882/1‑140 (MQ=255)

                                                                                                                       tGAAGGTCTGAACGTACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCg                <  2:3013344/140‑1 (MQ=255)

                                                                                                                                     tACCCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGt  <  2:1211667/140‑1 (MQ=255)

ATGGCCGACTCCGGTGCGCGTGGTTCTGCGGCACAGATTCGTCAGCTTGCTGGTATGCGTGGTCTGATGGCGAAGCCGGATGGCTCCATCATCGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGT  >  CP009273/4177450‑4177722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A