Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F55 I0 R1 2523 96.7 4820918 92.8% 4473811 133.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,229,246 C→G 20.5% intergenic (‑140/+36) PP_4606 ← / ← PP_4607 ferric siderophore receptor/transmembrane sensor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,229,2460CG20.5% 173.8 / 44.3 78intergenic (‑140/+36)PP_4606/PP_4607ferric siderophore receptor/transmembrane sensor
Reads supporting (aligned to +/- strand):  ref base C (32/30);  new base G (9/7);  total (41/37)
Fisher's exact test for biased strand distribution p-value = 7.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.07e-02

TGAACTTCATGGACGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTCGGCCACCGGGAAGCTGCCGCTGAC  >  NC_002947/5229097‑5229392
                                                                                                                                                     |                                                                                                                                                   
tGAACTTCATGGACGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACTCGCCCCTAc                                                                                                                                                     >  1:947823/1‑150 (MQ=255)
 gAACTTCATGGACGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACg                                                                                                                                                    <  1:379797/150‑1 (MQ=255)
  aaCTTCATGGACGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACgg                                                                                                                                                   <  1:693083/150‑1 (MQ=255)
  aaCTTCATGGACGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACgg                                                                                                                                                   <  2:1699211/150‑1 (MQ=255)
   aCTTCATGGACGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACggg                                                                                                                                                  <  2:1044124/150‑1 (MQ=255)
     ttCATGGACGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACATGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACggg‑gg                                                                                                                                               >  2:2121755/1‑150 (MQ=255)
     ttCATGGACGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACggg‑gg                                                                                                                                               <  1:2239999/150‑1 (MQ=255)
     ttCATGGACGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGGGCTGTTACCTCACCTGTGCCTTGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACggg‑gg                                                                                                                                               <  2:438719/150‑1 (MQ=255)
      ttaTGGACGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATACTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACggg‑ggg                                                                                                                                              <  1:1316901/148‑1 (MQ=255)
      tCATGGACGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACggg‑ggg                                                                                                                                              <  1:1092821/150‑1 (MQ=255)
       cATGGACGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGt                                                                                                                                             <  2:1734357/150‑1 (MQ=255)
          ggACGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCACGGGTAAACCC‑CCCGTAGGGGCGGGTTTa                                                                                                                                          <  2:2249799/150‑1 (MQ=38)
            ccGCAGAACTGTGAGGGGGTGATTATTGAGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTCCCTCACCTGTGCATGGAAATCTTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTAcc                                                                                                                                        <  2:104354/149‑1 (MQ=255)
                aGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTg                                                                                                                                    >  1:2035219/1‑150 (MQ=255)
                  aCCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGaa                                                                                                                                  <  1:959652/150‑1 (MQ=255)
                    cTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCACGGGTAAACCC‑CCCGTAGGGGCGGGTTTACCCGCGaa                                                                                                                                  <  1:1793216/148‑1 (MQ=37)
                    cTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCACGGGTAAACCC‑CCCGTAGGGGCGGGTTTACCCGCGaa                                                                                                                                  >  2:1793216/1‑148 (MQ=37)
                     tgtgAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAagag                                                                                                                               >  1:274846/1‑150 (MQ=255)
                         aGGGGGTGATTATTGCGAATCGATCGCACTTTTACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTg                                                                                                                           <  2:235651/150‑1 (MQ=255)
                         aGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACATGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTg                                                                                                                           <  1:2121755/150‑1 (MQ=255)
                            gggTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCACGGGTAAACCC‑CCCGTAGGGGCGGGTTTAc                                                                                                                                         <  1:1360505/133‑1 (MQ=38)
                            gggTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCACGGGTAAACCC‑CCCGTAGGGGCGGGTTTAc                                                                                                                                         >  2:1360505/1‑133 (MQ=38)
                              gTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACggg                                                                                                                                                  >  2:1934925/1‑123 (MQ=255)
                              gTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACggg                                                                                                                                                  <  1:1934925/123‑1 (MQ=255)
                              gTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGCGAAGAGGCTGGcaca                                                                                                                      >  2:1674883/1‑150 (MQ=255)
                              gTGATTATTGCGAATCAATAGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGAAAACCCGCCCCTACGGG‑GGGCTTACCGGTGAAGAGCCTGGcaca                                                                                                                      >  2:389762/1‑150 (MQ=255)
                               tGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAg                                                                                                                     <  1:2010229/150‑1 (MQ=255)
                                       gCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTAc                                                                                                             <  2:2035219/150‑1 (MQ=255)
                                         gAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCACGGGTAAACCC‑CCCGTAGGGGCGGGTTTACCCg                                                                                                                                      <  1:279707/123‑1 (MQ=37)
                                         gAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCACGGGTAAACCC‑CCCGTAGGGGCGGGTTTACCCg                                                                                                                                      >  2:279707/1‑123 (MQ=37)
                                                      tttCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCaaa                                                                                              >  2:2117451/1‑150 (MQ=255)
                                                           cTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGccc                                                                                         <  2:2119920/150‑1 (MQ=255)
                                                           cTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGccc                                                                                         >  2:1450353/1‑150 (MQ=255)
                                                            tCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCaa                                                                                                                 >  2:1189418/1‑125 (MQ=255)
                                                            tCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCaa                                                                                                                 <  1:1189418/125‑1 (MQ=255)
                                                            tCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGGTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGcccc                                                                                        >  1:819597/1‑150 (MQ=255)
                                                            tCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGCCAGCCTCTTCACGGGTAAACCC‑CCCGTAGGGGCGGGTTTAcc                                                                                                                                        >  1:835495/1‑102 (MQ=18)
                                                            tCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGCCAGCCTCTTCACGGGTAAACCC‑CCCGTAGGGGCGGGTTTAcc                                                                                                                                        <  2:835495/102‑1 (MQ=18)
                                                                       aTGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTg                                                                             >  1:1486184/1‑150 (MQ=255)
                                                                           cACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAagag                                                                                                                               >  2:2409215/1‑96 (MQ=255)
                                                                           cACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAagag                                                                                                                               <  1:2409215/96‑1 (MQ=255)
                                                                           cACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGc                                                                                           >  2:1927367/1‑132 (MQ=255)
                                                                           cACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGc                                                                                           <  1:1927367/132‑1 (MQ=255)
                                                                           cACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGt                                                                         >  1:875749/1‑150 (MQ=255)
                                                                           cACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGt                                                                         >  1:1417295/1‑150 (MQ=255)
                                                                               tGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGaa                                                                                                                                  <  2:2273682/89‑1 (MQ=255)
                                                                               tGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGaa                                                                                                                                  >  1:2273682/1‑89 (MQ=255)
                                                                                   gTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAAcc                                                                 >  2:961405/1‑150 (MQ=255)
                                                                                   gTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACACGCGCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAAcc                                                                 <  1:1674883/150‑1 (MQ=255)
                                                                                    ttACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAAccc                                                                <  2:819597/150‑1 (MQ=255)
                                                                                      aCCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCGAACGCCCCAACACGGTCTGCCGTGAAAAACCCAt                                                              >  1:1383887/1‑150 (MQ=255)
                                                                                        cTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGC‑GGGTTTACCCGTGAAGAGGCCGGCACAGGCAACTACCGAACCAGGCTCAAACGCCCCAACACGGTCTGCCGTGAACAACCCATCa                                                            >  1:632775/1‑150 (MQ=255)
                                                                                         tCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAgg                                                                                                    <  2:975561/109‑1 (MQ=255)
                                                                                         tCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAgg                                                                                                    >  1:975561/1‑109 (MQ=255)
                                                                                          cACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCAcc                                                          <  1:1450353/150‑1 (MQ=255)
                                                                                               gtgCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGcc                                                     <  2:61337/150‑1 (MQ=255)
                                                                                                   cTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGc                                                 >  1:1000951/1‑150 (MQ=255)
                                                                                                     ggAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCC‑CCCGTAGGGGCGGGTTTAcc                                                                                                                                        <  2:1529367/61‑1 (MQ=14)
                                                                                                     ggAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCC‑CCCGTAGGGGCGGGTTTAcc                                                                                                                                        >  1:1529367/1‑61 (MQ=14)
                                                                                                        aaTATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATc                                            >  1:1487767/1‑150 (MQ=255)
                                                                                                           atTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAg                                         >  1:2212763/1‑150 (MQ=255)
                                                                                                            tttttGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGCAGCGCCTATCACCTTGCCCAGCGAATCCAgc                                        <  1:1642877/150‑1 (MQ=255)
                                                                                                                  cTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCTAATTAGTGGCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCcagc                                  >  1:294167/1‑150 (MQ=255)
                                                                                                                   tgtgGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCcagca                                 >  2:2074408/1‑150 (MQ=255)
                                                                                                                       gcggcCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCgg                             >  1:1260690/1‑150 (MQ=255)
                                                                                                                             tctTCACGGGTAAACCC‑CCCGTAGGGGCGGGTTTACCCGCGAAGAGGCCGCCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTCGGc                       >  1:653477/1‑150 (MQ=25)
                                                                                                                               ttCACGGGTAAACCC‑CCCGTAGGGGCGGGTTTACCCGCGAAGAGTCTCGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTCGGCCa                     >  2:2269291/1‑150 (MQ=37)
                                                                                                                                    gggTAAACCC‑CCCGTAGGGGCGGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTg                                                                        <  2:306195/94‑1 (MQ=25)
                                                                                                                                    gggTAAACCC‑CCCGTAGGGGCGGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTg                                                                        >  1:306195/1‑94 (MQ=25)
                                                                                                                                    gggTAAACCC‑CCCGTAGGGGCGGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTCGGCCACCggg                >  1:2155463/1‑150 (MQ=39)
                                                                                                                                      gTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTCGGCCACCGGGaa              <  2:947823/150‑1 (MQ=255)
                                                                                                                                        aaaCCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTCGGCCACCGGGAAGc            >  2:1489623/1‑150 (MQ=255)
                                                                                                                                           cccGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTCGGCCACCGGGAAGCTGc         <  1:2117451/150‑1 (MQ=255)
                                                                                                                                            ccGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTCGGCCACCGGGAAGCTGcc        >  1:812747/1‑150 (MQ=255)
                                                                                                                                                cccTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCCGCGGCTCGGCCACCGGGAAGCTGCCGCTg    >  1:521569/1‑150 (MQ=255)
                                                                                                                                                cccTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTCGGCCACGGGGAAGCTGCCGCTg    >  2:1681902/1‑150 (MQ=255)
                                                                                                                                                  cTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTCGGCCACCGGGAAGCTGCCGCTGAc  >  2:2176833/1‑150 (MQ=255)
                                                                                                                                                  cTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTCGGCCACCGGGAAGCTGCCGCTGAc  >  1:1378429/1‑150 (MQ=255)
                                                                                                                                                  cTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTCGGCCACCGGGAAGCTGCCGCTGAc  <  1:961405/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TGAACTTCATGGACGCAGACCTGTGAGGGGGTGATTATTGCGAATCAATCGCACTTTCACTCATTACACGGATGCCACGTGCTGTTACCTCACCTGTGCCTGGAAATATTTTTGCTGTGGCGGCCTCTTCGCGGGTAAACCCGCCCCTACGGG‑GGGTTTACCCGTGAAGAGGCTGGCACAGGCAACTACCGAACCAGGGTCAAACGCCCCAACACGGTCTGCCGTGAAAAACCCATCACCTTGCCCAGCGAATCCAGCGCCAGCAGCGGCTCGGCCACCGGGAAGCTGCCGCTGAC  >  NC_002947/5229097‑5229392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A