Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F60 I0 R1 1078 79.7 3885236 94.2% 3659892 134.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,458,940:1 +A 22.2% intergenic (+17/‑45) leuS → / → PP_4795 leucine‑‑tRNA ligase/lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,458,9401.A22.2% 198.7 / 78.1 81intergenic (+17/‑45)leuS/PP_4795leucine‑‑tRNA ligase/lipoprotein
Reads supporting (aligned to +/- strand):  ref base . (31/32);  new base A (8/10);  total (39/42)
Fisher's exact test for biased strand distribution p-value = 7.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.08e-01

GCCGCTGCCAGCCGCGAAGAAGTCGAAGCCGCTGCCCGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCACCGGCGCCACCGAGCTGAGCGTGAAGGAAAT  >  NC_002947/5458792‑5459088
                                                                                                                                                       |                                                                                                                                                    
gccGCTGCCAGCCGCGAAGAAGTCGAAGCCGCTGCCCGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGC‑a                                                                                                                                                     >  1:206655/1‑150 (MQ=255)
    cTGCCAGCCGCGAAGAAGTCGAAGCCGCTGCCCGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGC‑ATCGa                                                                                                                                                 <  2:708835/150‑1 (MQ=255)
       ccAGCCGCGAAGAAGTCGAAGCCGCTGCCCGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGCAATCGAtg                                                                                                                                               <  2:960336/150‑2 (MQ=255)
        cAGCCGCGAAGAAGTCGAAGCCGCTGCCCGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGC‑ATCGATTGc                                                                                                                                             <  1:215763/150‑1 (MQ=255)
          gCCGCGAAGAAGTCGAAGCCGCTGCCCGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGC‑ATCGATTGCgg                                                                                                                                           <  2:642673/150‑1 (MQ=255)
           ccGCGAAGAAGTCGAAGCCGCTGCCCGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGC‑ATCGATTGCggg                                                                                                                                          >  1:947040/1‑150 (MQ=255)
             gcgAAGAAGTCGAAGCCGCTGCCCGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGC‑ATCGATTGCGGGtt                                                                                                                                        <  1:1342564/150‑2 (MQ=255)
              cgAAGAAGTCGAAGCCGCTGCCCGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGCAATCGA‑TGCGGGTgg                                                                                                                                       <  1:1740047/150‑1 (MQ=255)
                     gTCGAAGCCGCTGCCCGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGCAATCGA‑TGCGGGTGGGCgtttt                                                                                                                                <  1:538976/150‑5 (MQ=255)
                     gTCGAAGCCGCTGCCCGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGC‑ATCGATTGCGGGTGGGCGGAAc                                                                                                                                >  1:1529022/1‑150 (MQ=255)
                        gAAGCCGCTGCCCGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGC‑ATCGATTGCGGGTGGGCGGAACAgg                                                                                                                             >  2:1459482/1‑150 (MQ=255)
                        gAAGCCGCTGCCCGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGC‑ATCGATTGCGGGTGGGCGGAACAgg                                                                                                                             >  2:864666/1‑150 (MQ=255)
                              gCTGCCCGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCaaa                                                                                                                       >  1:1898467/1‑150 (MQ=255)
                                   ccGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGC‑ATCGATTGCGGGTGGGCGGAACAgg                                                                                                                             >  1:1512380/1‑139 (MQ=255)
                                   ccGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGC‑ATCGATTGCGGGTGGGCGGAACAgg                                                                                                                             <  2:1512380/139‑1 (MQ=255)
                                   ccGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGa                                                                                                                  >  1:743383/1‑150 (MQ=255)
                                   ccGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGa                                                                                                                  <  2:1898467/150‑1 (MQ=255)
                                    cGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAg                                                                                                                 <  1:1459482/150‑1 (MQ=255)
                                    cGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAg                                                                                                                 <  2:206655/150‑1 (MQ=255)
                                    cGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTAGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAg                                                                                                                 <  1:1581571/150‑1 (MQ=255)
                                     gcagcaACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGc                                                                                                                >  1:578391/1‑150 (MQ=255)
                                       agcaACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAATCGCACACC‑‑CGC‑ATCGATTGCGGGTGGGCGGAACAGGCTCAAAATGGAGcaa                                                                                                              <  2:1529022/150‑1 (MQ=255)
                                        gcaACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGCAATCGA‑TGCGGGTgg                                                                                                                                       >  2:1154058/1‑124 (MQ=255)
                                        gcaACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGCAATCGA‑TGCGGGTgg                                                                                                                                       <  1:1154058/124‑1 (MQ=255)
                                          aaCGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGC‑ATCGATTGCGGGTGGGCGGaa                                                                                                                                 >  1:745029/1‑128 (MQ=255)
                                          aaCGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGC‑ATCGATTGCGGGTGGGCGGaa                                                                                                                                 <  2:745029/128‑1 (MQ=255)
                                             gagaATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACAt                                                                                                        <  2:894288/150‑1 (MQ=255)
                                                aaTGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGC‑ATCGATTGCGGGTgg                                                                                                                                       >  1:1745279/1‑116 (MQ=255)
                                                aaTGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGC‑ATCGATTGCGGGTgg                                                                                                                                       <  2:1745279/116‑1 (MQ=255)
                                                       tGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGc                                                                                              >  2:579752/1‑150 (MQ=255)
                                                              cATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGc                                                                                       <  1:864666/150‑1 (MQ=255)
                                                                       ccTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGCAATCGA‑TGCGGGTgg                                                                                                                                       <  2:1636176/93‑1 (MQ=39)
                                                                       ccTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGCAATCGA‑TGCGGGTgg                                                                                                                                       >  1:1636176/1‑93 (MQ=39)
                                                                         tGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGc                                                                            <  2:578391/150‑1 (MQ=255)
                                                                             cacccGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTgg                                                                        >  1:1939896/4‑150 (MQ=255)
                                                                             cATCCGCGAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCaaa                                                                                                 <  2:1473857/125‑1 (MQ=255)
                                                                             cATCCGCGAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCaaa                                                                                                 >  1:1473857/1‑125 (MQ=255)
                                                                             cATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGCAATCGA‑TGCGGGTGGGCgtttt                                                                                                                                <  1:1022174/94‑5 (MQ=37)
                                                                             cATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGCAATCGA‑TGCGGGTGGGCgtttt                                                                                                                                >  2:1022174/1‑90 (MQ=37)
                                                                             cATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTgg                                                                        <  2:947040/150‑1 (MQ=255)
                                                                             cATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTgg                                                                        >  1:1353409/1‑150 (MQ=255)
                                                                                    aaGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCAt                                                                 >  2:1057654/1‑150 (MQ=255)
                                                                                      ggTCATCGTGTTACCGTGGAAGCTTGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCACCC‑ACCGA‑‑GGGCGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGc                                                               <  1:611802/150‑1 (MQ=255)
                                                                                         cATCGTGGTACCGGGCAAGCTGGTCAATATCGTCGCCAACTTATGAAAACACCCACC‑‑CGC‑ATCGATTGCGGGTGGGCGGAACAGGCTCAAAATGGAGCAACAACATGATCAAACGCAATTCGCTGGTAATCGGCCTGGCGGTCATGCTCa                                                            <  2:376475/150‑1 (MQ=255)
                                                                                           tCGTGGTACCTGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGC‑ATCGATTGCGGGTGGGCGCAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAgc                                                          <  1:252378/150‑1 (MQ=255)
                                                                                           tCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAgc                                                          <  2:1939896/150‑1 (MQ=255)
                                                                                             gTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAggg                                                                                                                   <  1:1029715/91‑1 (MQ=255)
                                                                                             gTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAggg                                                                                                                   >  2:1029715/1‑91 (MQ=255)
                                                                                                 tACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGc                                                    <  1:1057654/150‑1 (MQ=255)
                                                                                                 tACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGc                                                    >  1:608742/1‑150 (MQ=255)
                                                                                                   ccGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCAGCC‑‑CAA‑ACGAATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACCACATGATCGCACACAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCgg                                                  >  1:1506029/1‑150 (MQ=255)
                                                                                                      ggCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGttt                                               >  2:202391/1‑150 (MQ=255)
                                                                                                       gCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTc                                              >  1:697422/1‑150 (MQ=255)
                                                                                                          aGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAg                                           <  1:202391/150‑1 (MQ=255)
                                                                                                            cTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑TGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCt                                                              >  2:1457974/1‑129 (MQ=255)
                                                                                                            cTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑TGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCt                                                              <  1:1457974/129‑1 (MQ=255)
                                                                                                             tGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTg                                        >  1:376710/1‑150 (MQ=255)
                                                                                                             tGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTg                                        >  1:1279821/1‑150 (MQ=255)
                                                                                                               gTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTgcg                                      >  1:1457144/1‑150 (MQ=255)
                                                                                                                tCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTgcgc                                     >  2:445381/1‑150 (MQ=255)
                                                                                                                tCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATGTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTgcgc                                     >  2:218769/1‑150 (MQ=255)
                                                                                                                 cAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGcgcg                                    <  2:376710/150‑1 (MQ=255)
                                                                                                                    cATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCa                                 >  2:1534541/1‑150 (MQ=255)
                                                                                                                         tcgCCAACTGATGAAAACGCCCACC‑‑CGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCACCGgc                            <  2:1457144/150‑1 (MQ=255)
                                                                                                                                   atgaAAACGCCCACC‑‑CGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTgg                                                                        <  2:652323/96‑1 (MQ=255)
                                                                                                                                   atgaAAACGCCCACC‑‑CGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTgg                                                                        >  1:652323/1‑96 (MQ=255)
                                                                                                                                   atgaAAACGCCCACC‑‑CGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCGTGCGGTGTCCAGCTGCGCGGCACCGGCGCCCCTGAGc                  >  2:350083/1‑150 (MQ=255)
                                                                                                                                      aaaaCGCCCACC‑‑CGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCCCGATGTAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCACCGGCGCCACCGAGCTGa               <  1:1180912/150‑1 (MQ=255)
                                                                                                                                       ttccGCCCACC‑‑CGCAATCGA‑TGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGtt                                                <  2:1340233/113‑1 (MQ=39)
                                                                                                                                       ttccGCCCACC‑‑CGCAATCGA‑TGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGtt                                                >  1:1340233/4‑116 (MQ=39)
                                                                                                                                         aCGCCCACC‑‑CGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCACCGGCGCCACCGAGCTGAGCg            <  2:608742/150‑1 (MQ=255)
                                                                                                                                          cGCCCACC‑‑CGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTTATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCACCGGCGCCACCGAGCTGAGCGt           <  2:1252620/150‑1 (MQ=255)
                                                                                                                                          cGCCCACC‑‑CGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCACCGGCGCCACCGAGCTGAGCGt           <  1:218769/150‑1 (MQ=255)
                                                                                                                                            cccACC‑‑CGCAATCGA‑TGCTGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATg                                                                <  1:951642/95‑1 (MQ=255)
                                                                                                                                            cccACC‑‑CGCAATCGA‑TGCTGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATg                                                                >  2:951642/1‑95 (MQ=255)
                                                                                                                                            cccACC‑‑CGCAATCGA‑TGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCACCGGCGCCACCGAGCTGAGCGTGa         >  2:862284/1‑150 (MQ=255)
                                                                                                                                             ccACC‑‑CGCAATCGA‑TGCGGGTGGGCGTAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCCGCGGTTTCCAGCTGCGCGGCACCGGCGCCACCGAGCTGAGCg            >  2:458591/1‑146 (MQ=255)
                                                                                                                                             ccACC‑‑CGCAATCGA‑TGCGGGTGGGCGTAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCCGCGGTTTCCAGCTGCGCGGCACCGGCGCCACCGAGCTGAGCg            <  1:458591/146‑1 (MQ=255)
                                                                                                                                              cACC‑‑CGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCACCGGCGCCACCGAGCTGAGCGTGAAg       >  2:147835/1‑150 (MQ=255)
                                                                                                                                                cc‑‑cGCAATCGA‑TGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTgg                                                                        <  1:829253/83‑1 (MQ=39)
                                                                                                                                                cc‑‑cGCAATCGA‑TGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTgg                                                                        >  2:829253/1‑83 (MQ=39)
                                                                                                                                                     gC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCACCGGCGCCACCGAGCTGAGCGTGAAGGAAAt  >  1:753116/1‑150 (MQ=255)
                                                                                                                                                       |                                                                                                                                                    
GCCGCTGCCAGCCGCGAAGAAGTCGAAGCCGCTGCCCGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACC‑‑CGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCACCGGCGCCACCGAGCTGAGCGTGAAGGAAAT  >  NC_002947/5458792‑5459088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A