Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F35 I1 R1	5	55.4	2668064	94.0%	2507980	137.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_002947	1,851,823	(TGCG)_2→3	coding (867/2241 nt)	relA →	ATP:GTP 3'‑pyrophosphotransferase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_002947	= 1851813	5 (0.100)	37 (0.690) +CGTG	29/244	0.9	88.0%	coding (857/2241 nt)	relA	ATP:GTP 3'‑pyrophosphotransferase
*	?	NC_002947	1851814 =	5 (0.090)	37 (0.690) +CGTG	29/244	0.9	88.0%	coding (858/2241 nt)	relA	ATP:GTP 3'‑pyrophosphotransferase

CTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_002947/1851670‑1851813

‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCA  >  NC_002947/1851814‑1851957

                                                                                                                                                ||||

CTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCG                                                                                                                                                >  1:413416/1‑150

 TTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGT                                                                                                                                               >  2:1003764/1‑150

 TTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGT                                                                                                                                               >  2:131476/1‑150

  TCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTG                                                                                                                                              <  1:919395/150‑1

   CATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGC                                                                                                                                             >  2:832361/1‑150

     TCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTCGCTCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGT                                                                                                                                           <  1:14388/150‑1

     TCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGT                                                                                                                                           >  1:361135/1‑150

                  GAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGTGCGGTGCGCGTGC                                                                                                                              <  2:361135/150‑1

                  GAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAC                                                                                                                                                     <  1:356172/127‑1

                  GAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAC                                                                                                                                                     >  2:356172/1‑127

                                   AGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGC                                                                                                             <  1:131476/150‑1

                                        CTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGTGCTGTGCGCGTGCTTGTGCCGGAAATCCGCGACTG                                                                                                        >  2:218279/1‑150

                                         TCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTCGGTCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGTCGTGCGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGC                                                                                                       <  1:1167477/150‑1

                                             CACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACA                                                                                                   >  2:1092542/1‑150

                                                TGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCG                                                                                                >  2:499714/1‑150

                                                      GAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACA                                                                                                   <  1:1312508/141‑1

                                                      GAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACA                                                                                                   >  2:1312508/1‑141

                                                      GAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGAAAGATCTACGACGTGCGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGG                                                                                          <  1:1092542/150‑1

                                                         GGCCGACATCAGCGGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCA                                                                                       <  2:579567/150‑1

                                                          GCCGACATCAGCGGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCAT                                                                                      >  2:23084/1‑150

                                                            CGACATCAGCGGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCG                                                                                    >  1:898867/1‑150

                                                                  CAGCGGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAATTCAGCCAGATCTACGACGTGCGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACA                                                                              >  1:1017501/1‑150

                                                                   AGCGGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACAC                                                                             <  2:17934/150‑1

                                                                      GGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTT                                                                          <  1:816673/150‑1

                                                                       GCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTG                                                                         <  1:1003764/150‑1

                                                                              GAAACATACCTATTCCATCTGGCTCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGC                                                                  <  2:413416/150‑1

                                                                                 ACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATA                                                               <  1:23084/150‑1

                                                                                        TATTCGATCTGGCGCAAGATGCAGCGCAAAGGCATGCTTCTCAGCCAGATCTACGACGTGCGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAA                                                        <  1:1068404/150‑1

                                                                                         ATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGC                                                                                                             >  2:945303/1‑96

                                                                                         ATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGC                                                                                                             <  1:945303/96‑1

                                                                                          TTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTG                                                                                           <  1:8883/113‑1

                                                                                          TTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTG                                                                                           >  2:8883/1‑113

                                                                                                  GGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGAC                                              <  1:832361/150‑1

                                                                                                  GGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTACACACCTTGTGGCGGCATATTCCCAAGGAGTTCGAC                                              <  1:499714/150‑1

                                                                                                     GCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGAC                                           >  2:498951/1‑150

                                                                                                                   AAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAAGCCGCGGCTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAATCC                             <  2:371340/150‑1

                                                                                                                                 CAGCCAGATCTACGACGTGCGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCT               <  2:1017501/150‑1

                                                                                                                                              GACGTGCGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCA  >  1:482932/1‑150

                                                                                                                                                ||||

CTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_002947/1851670‑1851813

‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCA  >  NC_002947/1851814‑1851957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.

GATK/CNVnator alignment

N/A