Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F80 I0 R1	1148	65.4	3296958	93.8%	3092546	131.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	643,952	C→G	100%	M19I (ATG→ATC)	acoB ←	acetoin:2,6‑dichlorophenolindophenol oxidoreductase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	643,952	0	C	G	100.0%	257.2 / ‑6.8	77	M19I (ATG→ATC)	acoB	acetoin:2,6‑dichlorophenolindophenol oxidoreductase subunit beta
Reads supporting (aligned to +/- strand): ref base C (0/1); major base G (34/41); minor base A (1/0); total (35/42)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCGTCAAGCACGCGGCCGGGGAATTGGTGATACAGGCCTTTGGTCACGCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGCATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTG  >  NC_002947/643812‑644093

gcgTCAAGCACGCGGCCGGGGAATTGGTGATACAGGCCTTTGGTCACGCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTgg                                                                                                                                      <  2:108968/150‑1 (MQ=255)

      aGCACGCGGCCGGGGAATTGGTGATACAGGCCTTTGGTCACGCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAgg                                                                                                                                <  2:333117/150‑1 (MQ=255)

              gCCGGGGAATTGGTGATACAGGCCTTTGGTCACGCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGtt                                                                                                                        <  2:236728/150‑1 (MQ=255)

                 ggggAATTGGTGATACAGGCCTTTGGTCACGCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTcaaccag                                                                                                                     <  2:1605647/150‑7 (MQ=255)

                  gggAATTGGTGATACAGGCCTTTGGTCACGCCCCACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATg                                                                                                                    <  2:1364875/150‑1 (MQ=255)

                     aaTTGGTGATACAGGCCTTTGGTCATGCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGtt                                                                                                                        >  2:987523/1‑143 (MQ=255)

                     aaTTGGTGATACAGGCCTTTGGTCATGCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGtt                                                                                                                        <  1:987523/143‑1 (MQ=255)

                     aaTTGGTGATACAGGCCTTTGGTCACGCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATTGTGAATACTGTCCGGTCGCTGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGcc                                                                                                                 <  2:1291212/150‑1 (MQ=255)

                            gATACAGGCCTTTGGTCACGCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGTCCAGGGCCTCGTTGATGGCTTGCTGGt                                                                                                          <  2:459856/150‑1 (MQ=255)

                             aTACAGGCCTTTGGTCACGCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGCATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTa                                                                                                         <  1:1015121/150‑1 (MQ=255)

                                cAGGCCTTTGGTCACGCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGc                                                                                                       <  1:1239389/149‑1 (MQ=255)

                                cAGGCCTTTGGTCACGCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGc                                                                                                       >  2:1239389/1‑149 (MQ=255)

                                 aGGCCTTTGGTCACGCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTg                                                                                                     >  1:894486/1‑150 (MQ=255)

                                 aGGCCTTTGGTCACGCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTg                                                                                                     >  1:1617675/1‑150 (MQ=255)

                                   gCCTTTGGTCACGCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGAt                                                                                                   >  1:1002280/1‑150 (MQ=255)

                                     cTTTGGTCACGCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGAtct                                                                                                 >  2:1537704/1‑150 (MQ=255)

                                         ggTCACGCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGAtctttct                                                                                             <  2:1242792/150‑1 (MQ=255)

                                             aCGCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGcc                                                                                         >  2:787370/1‑150 (MQ=255)

                                               gCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGc                                                                                                                  >  1:294176/1‑123 (MQ=255)

                                               gCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGc                                                                                                                  <  2:294176/123‑1 (MQ=255)

                                               gCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCAt                                                                                       >  1:989940/1‑150 (MQ=255)

                                                 ccAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCctgct                                                                                                             <  2:1451010/126‑1 (MQ=255)

                                                 ccAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCctgct                                                                                                             >  1:1451010/1‑126 (MQ=255)

                                                  cAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCAtggt                                                                                    >  1:172340/1‑150 (MQ=255)

                                                  cAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCAtggt                                                                                    >  1:1425019/1‑150 (MQ=255)

                                                   aaCACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCAtggtg                                                                                   <  2:405744/150‑1 (MQ=255)

                                                   aaCACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCAtggtg                                                                                   >  1:405744/1‑150 (MQ=255)

                                                   aaCACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCAtggtg                                                                                   >  2:933557/1‑150 (MQ=255)

                                                    acacGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCAtggtgg                                                                                  <  2:990932/150‑1 (MQ=255)

                                                     cacGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATggtggt                                                                                 >  1:555583/1‑150 (MQ=255)

                                                        gCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTATCACGATGAAGGGCCTTGGCCAGGTCCTCGTTGATGGCATGACGCTAGCGGATGCTTCGACCCATGGTGGTtct                                                                              >  1:29248/1‑150 (MQ=255)

                                                         ccACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTtctc                                                                             <  2:555583/150‑1 (MQ=255)

                                                         ccACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATCACATCCGCGAGCGCATCGTGGAGGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTtctc                                                                             <  2:56294/150‑1 (MQ=255)

                                                          cACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGt                                                                                                                         <  1:1272927/105‑1 (MQ=255)

                                                          cACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGt                                                                                                                         >  2:1272927/1‑105 (MQ=255)

                                                          cACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCAtggtgg                                                                                  <  2:1393922/144‑1 (MQ=255)

                                                          cACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCAtggtgg                                                                                  >  1:1393922/1‑144 (MQ=255)

                                                           aCCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCt                                                                           <  2:1002280/150‑1 (MQ=255)

                                                              ccAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCtgtt                                                                        <  2:989940/150‑1 (MQ=255)

                                                               cAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCtgttg                                                                       >  2:733362/1‑150 (MQ=255)

                                                                    gTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCtt                                                                  <  2:849429/150‑1 (MQ=255)

                                                                    gTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCtt                                                                  <  1:764433/150‑1 (MQ=255)

                                                                       aTCCTCGCCGGGCGCACCGGCACAGCCGGCGACGTCTGCGCCCATGCCGACTAGTCTGCTGTCGCGGCGGATTTCCTGGGGCAGGGCCTCGTTGATGGCCAGGTGGTAGCTGATATTTCTTGCCATGGTGGTTGTCGTGTTGTTCTTGTa                                                               >  2:479755/1‑150 (MQ=255)

                                                                        tCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCt                                                                   >  2:934456/1‑145 (MQ=255)

                                                                        tCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCATGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCt                                                                   <  1:934456/145‑1 (MQ=255)

                                                                             gCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCATGTTGTTCTTGTAATcctc                                                         >  2:860351/1‑148 (MQ=255)

                                                                                    gcaccggcaccgCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAg                                                  <  1:933557/150‑1 (MQ=255)

                                                                                      accggcaccgCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAggg                                                >  1:939245/1‑150 (MQ=255)

                                                                                          gcaccgCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAg                                            <  2:894486/150‑1 (MQ=255)

                                                                                              cgccgGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGa                                        >  2:1225198/1‑150 (MQ=255)

                                                                                                 cgGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGGGACGt                                     >  1:749165/1‑150 (MQ=255)

                                                                                                 cgGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGt                                     <  1:1537704/150‑1 (MQ=255)

                                                                                                   gCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAg                                   <  2:172340/150‑1 (MQ=255)

                                                                                                      aCGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGAtcttt                                                                                               >  1:1362308/1‑87 (MQ=255)

                                                                                                      aCGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGAtcttt                                                                                               <  2:1362308/87‑1 (MQ=255)

                                                                                                      aCGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCAGCGGCTCAGGGGTAGGCGACGTAGACg                                >  2:155525/1‑150 (MQ=255)

                                                                                                           ttCGCCAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGt                           <  1:393806/150‑1 (MQ=255)

                                                                                                               ccAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAACCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGc                       <  2:947965/150‑1 (MQ=255)

                                                                                                                cAATGATGAATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCa                      <  1:1449948/150‑1 (MQ=255)

                                                                                                                      tggaTACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGAtctttctt                                                                                            >  1:1644791/4‑74 (MQ=255)

                                                                                                                      tgaATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGAtctttctt                                                                                            <  2:1644791/74‑1 (MQ=255)

                                                                                                                      tgaATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAg                                            >  2:309939/1‑122 (MQ=255)

                                                                                                                      tgaATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAg                                            <  1:309939/122‑1 (MQ=255)

                                                                                                                       gaATACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTcggc               >  2:1281966/1‑150 (MQ=255)

                                                                                                                        aaTACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTcggcg              >  2:1522554/1‑150 (MQ=255)

                                                                                                                         aTACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTcggcgg             <  1:1225198/150‑1 (MQ=255)

                                                                                                                          tACTGTGCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTTATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCggg            <  1:312680/150‑1 (MQ=255)

                                                                                                                             tgtgCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCtg         >  2:1409220/1‑150 (MQ=255)

                                                                                                                              gtgCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCtgt        <  1:1243468/150‑1 (MQ=255)

                                                                                                                              gtgCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCtgt        <  2:671791/150‑1 (MQ=255)

                                                                                                                                gCTGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTgg      <  1:733362/150‑1 (MQ=255)

                                                                                                                                  tGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCt    >  2:317991/1‑150 (MQ=255)

                                                                                                                                  tGTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCt    <  1:1409220/150‑1 (MQ=255)

                                                                                                                                   gTCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCtt   >  2:1318487/1‑150 (MQ=255)

                                                                                                                                    tCGCGGCGGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTg  <  1:1318487/150‑1 (MQ=255)

                                                                                                                                        ggcgGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAg                                                  >  2:588859/1‑98 (MQ=255)

                                                                                                                                        ggcgGATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAg                                                  <  1:588859/98‑1 (MQ=255)

GCGTCAAGCACGCGGCCGGGGAATTGGTGATACAGGCCTTTGGTCACGCCCAACACGCCACCCCAGGCGTCATCCTCGCCGGGCGCACCGGCACCGCCGGCGACGTCTTCGCCAATGATGAATACTGTGCTGTCGCGGCGCATTTCCTGGGCCAGGGCCTCGTTGATGGCCTGCTGGTAGCTGATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTG  >  NC_002947/643812‑644093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A