Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F80 I0 R1	1148	65.4	3296958	93.8%	3092546	131.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,013,231	A→T	10.1%	intergenic (+56/+75)	prfC → / ← potF‑I	peptide chain release factor 3/putrescine‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,013,231	0	A	T	10.1%	177.6 / 8.7	59	intergenic (+56/+75)	prfC/potF‑I	peptide chain release factor 3/putrescine‑binding protein
Reads supporting (aligned to +/- strand): ref base A (31/22); new base T (4/2); total (35/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

CTGACCTACCTGGCGCCGACCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCA  >  NC_002947/1013083‑1013376

cTGACCTACCTGGCGCCGACCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGaa                                                                                                                                                  >  2:493734/1‑150 (MQ=255)

     ctacctGGCGCCGACCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCAcc                                                                                                                                             >  1:588541/1‑150 (MQ=255)

           ggCGCCGACCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCt                                                                                                                                       <  1:173463/150‑1 (MQ=255)

                   ccggggTCAACCTGGCGCTGATGGAAGAGCGCGGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCa                                                                                                                               <  2:707466/147‑1 (MQ=255)

                    ccGGGTCAACCTGGCGCTGATGGAAGAGCGCTGACCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCAt                                                                                                                              >  1:520314/1‑150 (MQ=255)

                     cGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCAtg                                                                                                                             <  1:1164618/150‑1 (MQ=255)

                      gggTCAACCTGGCGCTGATGGAAGAGCGCTGACCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCAtgt                                                                                                                            <  2:520314/150‑1 (MQ=255)

                            aCCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACACGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATAcc                                                                                                                      >  2:150969‑M2/1‑148 (MQ=255)

                             ccTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCAc                                                                                                                                              >  2:203913/1‑125 (MQ=255)

                             ccTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCAc                                                                                                                                              <  1:203913/125‑1 (MQ=255)

                                 gcgcTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCACACCTGGAGGTCATGTGATAcctgtgg                                                                                                                 >  1:493734‑M2/1‑143 (MQ=255)

                                   gcTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATAcctgtggga                                                                                                               >  2:304582‑M2/1‑141 (MQ=255)

                                       aTGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCCCATGACCTCCAGGTCTGGTGATTcctgtgggagcgg                                                                                                           >  1:436024‑M2/1‑137 (MQ=255)

                                       aTGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATAcctgtgggagcgg                                                                                                           >  1:9989‑M2/1‑137 (MQ=255)

                                                       cGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATAcctgtgggagcgggcgtgcccgcgaaggg                                                                                           <  2:1339088‑M2/150‑30 (MQ=255)

                                                            aTCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCAtg                                                                                                                             >  2:1030789/1‑111 (MQ=255)

                                                            aTCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCAtg                                                                                                                             <  1:1030789/111‑1 (MQ=255)

                                                            aTCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATAcctgtg                                                                                                                  <  2:694667‑M2/122‑7 (MQ=255)

                                                            aTCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATAcctgtg                                                                                                                  >  1:694667‑M2/1‑116 (MQ=255)

                                                               aaGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCAtgt                                                                                                                            >  1:281688/1‑109 (MQ=255)

                                                               aaGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCAtgt                                                                                                                            <  2:281688/109‑1 (MQ=255)

                                                                     cgcgCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGccc                                                                             <  2:588541/150‑1 (MQ=255)

                                                                               gcgAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATAcctgtgggagcggg                                                                                                          <  2:136260‑M2/111‑15 (MQ=255)

                                                                               gcgAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATAcctgtgggagcggg                                                                                                          >  1:136260‑M2/1‑97 (MQ=255)

                                                                                     aCCATTAAGGCCTGAAATCGGGGCTTCTTTTCAACCCTTCGCGGGCACTCCCGCTCCCACCGGAATAACCAGACCTTGAGGTCATGGGATACCTGTGAGTGCCGGAGTGCCCGCGAAGGGCCGCTAGGCGGGCCCATTTCAATTTCCGGc                                                             >  2:764319/1‑150 (MQ=255)

                                                                                     aCCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTTATAcctgtgggagcctcc                                                                                                         >  2:1330351‑M2/1‑91 (MQ=255)

                                                                                     aCCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTTATAcctgtgggagcctcc                                                                                                         <  1:1330351‑M2/106‑16 (MQ=255)

                                                                                      gcATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGACCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCt                                                            >  2:1126196/2‑150 (MQ=255)

                                                                                             ggCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCAtgt                                                                                                                            <  1:594033/79‑1 (MQ=255)

                                                                                             ggCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCAtgt                                                                                                                            >  2:594033/1‑79 (MQ=255)

                                                                                                            ctgctTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTcgcg                                      >  1:1522438/1‑150 (MQ=255)

                                                                                                            ctgatTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGGGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGACACTGGTCCAGATTcgcg                                      >  2:482372/1‑150 (MQ=255)

                                                                                                             tgctTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCAt                                                                                                                              >  2:1097330/1‑61 (MQ=255)

                                                                                                             tgctTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCAt                                                                                                                              <  1:1097330/61‑1 (MQ=255)

                                                                                                                    cAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAGTa                              >  1:1641052/1‑150 (MQ=255)

                                                                                                                      gCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATAcctgtg                                                                                                                  >  2:1129869‑M2/1‑58 (MQ=255)

                                                                                                                      gCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATAcctgtg                                                                                                                  <  1:1129869‑M2/64‑7 (MQ=255)

                                                                                                                               gggCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCtt                   >  2:1581436/1‑150 (MQ=18)

                                                                                                                                      cccGCTCCCACAGGTATCACCAGACCTGGAGGTCATGTGa                                                                                                                          >  2:1148951/1‑40 (MQ=37)

                                                                                                                                      cccGCTCCCACAGGTATCACCAGACCTGGAGGTCATGTGa                                                                                                                          <  1:1148951/40‑1 (MQ=255)

                                                                                                                                       ccGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAg                                                                                    >  1:1348098/1‑77 (MQ=255)

                                                                                                                                       ccGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAg                                                                                    <  2:1348098/77‑1 (MQ=255)

                                                                                                                                            cccACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGcc      <  1:1339088/150‑1 (MQ=255)

                                                                                                                                             ccACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCt     >  1:958947/1‑150 (MQ=255)

                                                                                                                                             ccACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCt     >  2:1315189/1‑150 (MQ=255)

                                                                                                                                              cacaGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGAt                      >  2:883788/1‑132 (MQ=18)

                                                                                                                                              cacaGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGAt                      <  1:883788/132‑1 (MQ=18)

                                                                                                                                              cacaGGAATCACCAGACCTGGAGGTCATGTGATAcctgtgggagcgggcgtgcccgcgaagggctgcaaagcagccccgatttcaggccttaatggtgctcgcgggtagcgcggaacttgatgtccggccagcgctcttccatcagcgcc    <  2:735602‑M2/150‑117 (MQ=255)

                                                                                                                                               acaGGAATCACCAGACCTGGAGGTCATGTGATAcctgtgggagcgggcgtgcccgcgaagggctgcaaagcagccccgatttcaggccttaatggtgctcgcgggtagcgcggaacttgatgtccggccagcgctcttccatcagcgcca   <  2:921065‑M2/150‑118 (MQ=255)

                                                                                                                                               acaGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGt                                                 <  2:1240866/104‑1 (MQ=255)

                                                                                                                                               acaGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGt                                                 >  1:1240866/1‑104 (MQ=255)

                                                                                                                                               acaGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTcc   >  1:468039/1‑150 (MQ=255)

                                                                                                                                               acaGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTcc   >  1:1134314/1‑150 (MQ=255)

                                                                                                                                                caGGAATCACCAGACCTGGAGGTCATGTGATAcctgtgggagcgggcgtgcccgcgaagggctgcaaagcagccccgatttcaggccttaatggtgctcgcgggtagcgcggaacttgatgtccggccagcgctcttccatcagcgccag  >  1:921065‑M2/1‑32 (MQ=255)

                                                                                                                                                caGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATCCGGGCGATCTTCGCCGGCATCGCCTCCa  >  1:1072665/1‑150 (MQ=255)

                                                                                                                                                caGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCa  >  1:1602463/1‑150 (MQ=255)

                                                                                                                                                 aGGAATCACCAGACCTGGAGGTCATGTGATAcctgtgggagcgggcgtgcccgcgaagggctgcaaagcagccccgatttcaggccttaatggtgctcgcgggtagcgcggaacttgatgtccggccagcgctcttc              <  2:1143618‑M2/137‑107 (MQ=255)

                                                                                                                                                 aGGAATCACCAGACCTGGAGGTCATGTGATAcctgtgggagcgggcgtgcccgcgaagggctgcaaagcagccccgatttcaggccttaatggtgctcgcgggtagcgcggaacttgatgtccggccagcgctcttc              >  1:1143618‑M2/1‑31 (MQ=255)

                                                                                                                                                   gAATCACCAGACCTGGAGGTCATGTGATAcctgtgggagcgggcgtgcccgcgaagggctgcaaagcagccccgatttcaggccttaatggtgctcgcgggtagc                                            >  1:874585‑M2/1‑29 (MQ=255)

                                                                                                                                                   gAATCACCAGACCTGGAGGTCATGTGATAcctgtgggagcgggcgtgcccgcgaagggctgcaaagcagccccgatttcaggccttaatggtgctcgcgggtagc                                            <  2:874585‑M2/105‑77 (MQ=255)

                                                                                                                                                    aaTCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAAt                               >  2:463408/1‑117 (MQ=255)

                                                                                                                                                    aaTCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAAt                               <  1:463408/117‑1 (MQ=255)

CTGACCTACCTGGCGCCGACCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCA  >  NC_002947/1013083‑1013376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A