Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F80 I0 R1	1148	65.4	3296958	93.8%	3092546	131.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,851,994	G→T	100%	E346D (GAG→GAT)	relA →	ATP:GTP 3'‑pyrophosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,851,994	0	G	T	100.0%	152.9 / ‑6.8	47	E346D (GAG→GAT)	relA	ATP:GTP 3'‑pyrophosphotransferase
Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (26/20); minor base A (1/0); total (27/20)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGG  >  NC_002947/1851853‑1852135

cTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGAt                                                                                                                                       <  2:1480957/150‑1 (MQ=255)

      cacCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTAc                                                                                                                                 >  2:604318/1‑150 (MQ=255)

                 gCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGc                                                                                                                      <  2:768393/150‑1 (MQ=255)

                   aTCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCAc                                                                                                                    >  2:1405191/1‑150 (MQ=255)

                    tCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACg                                                                                                                   >  1:347634/1‑150 (MQ=255)

                            acCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGcc                                                                                                           >  1:1528878/1‑150 (MQ=255)

                                               ccAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGaa                                                                                                                 <  1:173495/125‑1 (MQ=255)

                                               ccAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGaa                                                                                                                 >  2:173495/1‑125 (MQ=255)

                                                    gAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGCACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTgg                                                                                   >  1:671450/1‑150 (MQ=255)

                                                     aGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGCACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGgc                                                                                  <  2:671450/150‑1 (MQ=255)

                                                            cgacTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACaa                                                                           >  2:1195479/1‑150 (MQ=255)

                                                              acTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAgg                                                                         <  2:1528878/150‑1 (MQ=255)

                                                              acTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAgg                                                                         <  2:300176/150‑1 (MQ=255)

                                                              acTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAgg                                                                         <  1:349501/150‑1 (MQ=255)

                                                               cTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGCGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAggg                                                                        >  2:1364024/1‑150 (MQ=255)

                                                                        cAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATgcgc                                                                                          >  2:602096/1‑123 (MQ=255)

                                                                        cAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATgcgc                                                                                          <  1:602096/123‑1 (MQ=255)

                                                                           ccccAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCaa                                                            <  1:1195479/150‑1 (MQ=255)

                                                                            cccAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGgc                                                                                  <  1:158289/127‑1 (MQ=255)

                                                                            cccAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGgc                                                                                  >  2:158289/1‑127 (MQ=255)

                                                                             ccAAGGAGAACGGCTACCGCTGGTGGCAGACTGCGGGAATCGGCCCCGAGGGCAAGGTGCTGGGTGTGCAGAGCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGc                                                          >  2:919999/1‑150 (MQ=255)

                                                                                aGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGc                                                                                                  <  1:1430687/107‑1 (MQ=255)

                                                                                aGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGc                                                                                                  >  2:1430687/1‑107 (MQ=255)

                                                                                aGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCa                                                       >  2:918754/1‑150 (MQ=255)

                                                                                     aaCGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTcc                                                  >  1:1449916/1‑150 (MQ=255)

                                                                                       cGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAagag                                                                                                              >  2:1623644/1‑88 (MQ=255)

                                                                                       cGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAagag                                                                                                              <  1:1623644/88‑1 (MQ=255)

                                                                                           tACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGAGACCACTGAAGCTGTGAGGGTATCGACGGCAAGCCCAGCTCCAACCAc                                            >  2:14701/1‑150 (MQ=255)

                                                                                              cGCTCGATGCACACTGCGGTAATCGGCCCCGAGGGCAAGGAGATGGAATGGCGGGTCCTGACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAAGGCACCGACTGCAAGCCGAGCTCCAACCACTAc                                         >  2:639273/1‑150 (MQ=255)

                                                                                               gCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACg                                        >  1:1272451/1‑150 (MQ=255)

                                                                                                          acTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGAtt                             >  1:72280/1‑150 (MQ=255)

                                                                                                            tGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAgg                                                                         >  1:179460/1‑104 (MQ=255)

                                                                                                            tGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAgg                                                                         <  2:179460/104‑1 (MQ=255)

                                                                                                            tGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTc                           >  1:1153788/1‑150 (MQ=255)

                                                                                                              cGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGaaaa                                 >  2:468727/1‑142 (MQ=255)

                                                                                                              cGGTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGaaaa                                 <  1:468727/142‑1 (MQ=255)

                                                                                                               ggTAATCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTg                        <  2:1272451/150‑1 (MQ=255)

                                                                                                                    tCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGgcgc                                                                                >  2:1569478/1‑89 (MQ=255)

                                                                                                                    tCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGgcgc                                                                                <  1:1569478/89‑1 (MQ=255)

                                                                                                                    tCGGCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGc                   >  2:1386939/1‑150 (MQ=255)

                                                                                                                       gCCCCGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTc                <  2:1449916/150‑1 (MQ=255)

                                                                                                                         cccGAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGCACTTGACATATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAg              <  1:604318/150‑1 (MQ=255)

                                                                                                                            gAGGGCAAGGTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCTAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTg           <  1:378333/150‑1 (MQ=255)

                                                                                                                                    ggTGCTGTATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTg   >  2:1100509/1‑150 (MQ=255)

                                                                                                                                     gTGCTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTgg  >  2:1554729/1‑150 (MQ=255)

                                                                                                                                        cTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTg                        >  2:656063/1‑125 (MQ=255)

                                                                                                                                        cTGGATGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTg                        <  1:656063/125‑1 (MQ=255)

CTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGG  >  NC_002947/1851853‑1852135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A