Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F80 I0 R1	1148	65.4	3296958	93.8%	3092546	131.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,796,672	C→T	9.9%	intergenic (‑185/+33)	pvdH ← / ← PP_4224	diaminobutyrate‑2‑oxoglutarate transaminase/sensor histidine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,796,672	0	C	T	9.9%	147.4 / 8.2	61	intergenic (‑185/+33)	pvdH/PP_4224	diaminobutyrate‑2‑oxoglutarate transaminase/sensor histidine kinase
Reads supporting (aligned to +/- strand): ref base C (26/28); new base T (3/3); total (29/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.63e-01

GACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATC  >  NC_002947/4796525‑4796810

gACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCAc                                                                                                                                           <  2:641847/150‑1 (MQ=255)

   aTGGGTACATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGACCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCcg                                                                                                                                        >  2:1302880/1‑150 (MQ=255)

   aTGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCcg                                                                                                                                        >  1:351439/1‑150 (MQ=255)

    tGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACGGGCGGGTTTACCcgc                                                                                                                                       <  2:1406468/150‑1 (MQ=38)

         aaaTCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGCTGTTACGCGATTTTATTTTTGTCTGTGCTTGCCTCTTCGCTGGTAAACCCTCCCGTAGGAGCTGCTTCACCCCCCAaga                                                                                                                                  >  2:165979/1‑150 (MQ=255)

            tCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGc                                                                                                                               <  1:1641027/150‑1 (MQ=255)

            tCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTGCACCCGCGAAGAGGc                                                                                                                               >  2:1141255/1‑150 (MQ=255)

               tCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAg                                                                                                                            >  2:518715/1‑150 (MQ=255)

                   gAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGcaca                                                                                                                        <  2:1468394/150‑1 (MQ=255)

                     gCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAgg                                                                                                                      <  1:750722/150‑1 (MQ=255)

                     gCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGGTCACCCGCGAAGAGGCCAGCACAgg                                                                                                                      >  1:155425/1‑150 (MQ=255)

                       aaGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAAGCCGCCGGTAGAAGCGGGTGCACCCGCGAAGAGGCCAGCACAGG‑Cg                                                                                                                   >  2:1334187/1‑150 (MQ=255)

                               ggggggTTTGCGCTTTTGAAACGCTTGAGTGGGGAGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAAACAt                                                                                                           <  2:723019/150‑1 (MQ=38)

                                    gTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATg                                                                                                      <  1:782208/150‑1 (MQ=255)

                                       aTCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACCCAGCGATATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGccc                                                                                                   <  1:1334187/150‑1 (MQ=38)

                                            tttGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCAccc                                                                                                                                         >  2:764454/1‑108 (MQ=255)

                                            tttGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCAccc                                                                                                                                         <  1:764454/108‑1 (MQ=255)

                                            tttGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGaa                                                                                                                                    <  1:1370239/113‑1 (MQ=255)

                                            tttGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGaa                                                                                                                                    >  2:1370239/1‑113 (MQ=255)

                                                gAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGATTCCAGCACAGG‑CAATAAACATGAATGCCCAGGAagcgg                                                                                          >  2:1319209/1‑149 (MQ=38)

                                                       ttGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTAcc                                                                                   >  1:1425134/1‑150 (MQ=255)

                                                        tGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGCGGGTTTACCCGCGAaga                                                                                                                                  >  1:459368/1‑103 (MQ=17)

                                                        tGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTGAACCCGCTCCTACGGGCGGGTTTACCCGCGAaga                                                                                                                                  <  2:459368/103‑1 (MQ=17)

                                                         gAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTg                                                                                 >  2:846889/1‑150 (MQ=255)

                                                            tGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGcaa                                                                              <  1:1048081/150‑1 (MQ=255)

                                                                   gggaTTTAGCGTTTGGTAGGCGATTTTATTCTTGTCTGTCCGGGCCTCTTCGCGAGTAAACCCGCGCGTAGGAGCGGGGTCACCCGAGAAGCGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAAcc                                                                       <  1:1410694/149‑1 (MQ=37)

                                                                   aggaTTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCAc                                                                                                                                           <  1:873073/83‑1 (MQ=255)

                                                                   aggaTTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCAc                                                                                                                                           >  2:873073/1‑83 (MQ=255)

                                                                       tttAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCCCAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCa                                                                   >  2:300639/1‑150 (MQ=255)

                                                                        ttAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCaa                                                                  <  1:846889/150‑1 (MQ=255)

                                                                           gCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGcc                                                               <  2:474524/150‑1 (MQ=255)

                                                                              gttgttACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAgg                                                                                                                      <  2:304293/93‑1 (MQ=255)

                                                                              gttgttACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAgg                                                                                                                      >  1:304293/1‑93 (MQ=255)

                                                                                    aCGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACg                                                      >  1:1287322/1‑150 (MQ=255)

                                                                                        gATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCGCGTAGGAGCGGGTTCACCCGCGAAGGGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTg                                                  <  1:1141255/150‑1 (MQ=255)

                                                                                         attttattttTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGt                                                 <  1:518715/150‑1 (MQ=255)

                                                                                          ttttattttTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAAACa                                                                                                            >  2:93046/1‑90 (MQ=255)

                                                                                          ttttattttTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAAACa                                                                                                            <  1:93046/90‑1 (MQ=255)

                                                                                                  ttGTCTGTGCTGCCCTCTGCGCGGGTAAACCCGCGCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCa                                        <  2:608077/150‑1 (MQ=255)

                                                                                                     tCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGc                                     >  1:1564006/1‑150 (MQ=255)

                                                                                                         tgCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTAcc                                 >  1:544274/1‑150 (MQ=255)

                                                                                                            tGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGc                              >  1:62032/1‑150 (MQ=255)

                                                                                                                ctctTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCacac                          <  2:1459294/150‑1 (MQ=255)

                                                                                                                 tctTCGCGGGTGAACCCGCTCCTACGGGCGGGTTTACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACAcc                         >  2:1071353/1‑150 (MQ=37)

                                                                                                                   ttCGCGGGTGAACCCGCTCCTACGGGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCg                                                              <  2:1467925/111‑1 (MQ=21)

                                                                                                                   ttCGCGGGTGAACCCGCTCCTACGGGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCg                                                              >  1:1467925/1‑111 (MQ=21)

                                                                                                                         ggTGAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACg                 >  2:1309492/1‑150 (MQ=255)

                                                                                                                           tgaaCCCGCTCCTACGGGCGGGTTTACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAACCCCAGCCTACCGCCCCACCGCTGCACGAt               >  2:253871/3‑150 (MQ=37)

                                                                                                                           tAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGAt               >  2:652893/1‑150 (MQ=255)

                                                                                                                             aaCCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGt                                                 >  2:1646989/1‑114 (MQ=255)

                                                                                                                             aaCCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGt                                                 <  1:1646989/114‑1 (MQ=255)

                                                                                                                              aCCCGCCCGTAGGAGCGGGTTCACCCGCGAAGTGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGc            <  2:544274/150‑1 (MQ=255)

                                                                                                                               cccgcccgTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGcc           <  2:155425/150‑1 (MQ=255)

                                                                                                                                ccgcccgTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAgc                                           <  2:419814/117‑1 (MQ=255)

                                                                                                                                ccgcccgTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAgc                                           >  1:419814/1‑117 (MQ=255)

                                                                                                                                ccgcccgTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCt          >  2:1415134/1‑150 (MQ=255)

                                                                                                                                  gcccgTAGGAGCGGGTTAACCCGCGAAGAGGCCAGC‑CACGCCGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGc        <  1:1319209/150‑1 (MQ=255)

                                                                                                                                   cccgTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGca       <  2:1564006/150‑1 (MQ=255)

                                                                                                                                        acgggCGGGTTTACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATc  <  1:1071353/146‑1 (MQ=255)

                                                                                                                                        aGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATc  <  2:62032/150‑1 (MQ=255)

                                                                                                                                        aGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATc  <  1:1309492/150‑1 (MQ=255)

GACATGGGTAAATCCTCGCGAGCAAGCGGATGGGCGGTTATCGCTTTGGAAACGCTTGAGTGGGGGGAGGATTTAGCGGTTGTTACGCGATTTTATTTTTGTCTGTGCTGGCCTCTTCGCGGGTAAACCCGCCCGTAGGAGCGGGTTCACCCGCGAAGAGGCCAGCACAGG‑CGATAAACATCAATGCCCAGGACGCGCCGGTACCTGCAACAAAACCCGCAAGCCGTCACTACGGCTGTCAAAGCGCAGGCTACCGGCACACCGCTGCACGATCGCCTGCACAATC  >  NC_002947/4796525‑4796810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A