Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F80 I0 R1 1105 125.4 6144758 94.6% 5812941 133.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 699,725 A→G 51.6% intergenic (+115/‑129) PP_t06 → / → PP_23SD tRNA‑Ala/23S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947699,7250AG51.6% ‑5.4 / 156.3 119intergenic (+115/‑129)PP_t06/PP_23SDtRNA‑Ala/23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (31/26);  new base G (26/35);  total (58/61)
Fisher's exact test for biased strand distribution p-value = 2.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.90e-01

Only 100 of 119 total aligned reads displayed.

GAGGTCAGCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTC  >  NC_002947/699577‑699807
                                                                                                                                                      |                                                                                  
gaggTCAGCGGTTCGATCCCGACTGGATCCTCTTCCTATGTTGTACGTAGTTAGGTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGAc                                                                                   <  2:807612/150‑1 (MQ=11)
 aggTCAGCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGGCt                                                                                  <  1:2776884/150‑1 (MQ=38)
 aggTCAGCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGGCt                                                                                  <  1:682067/150‑1 (MQ=255)
  ggTCAGCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGGCTg                                                                                 <  2:2631498/150‑1 (MQ=38)
    tCAGCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGACTGaa                                                                               >  1:1438587/1‑150 (MQ=39)
    tCAGCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGACTGaa                                                                               >  1:1810267/1‑150 (MQ=38)
      aGCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGGCTGAaca                                                                             >  1:2526270/1‑150 (MQ=255)
      aGCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGCCTGaaac                                                                             >  2:2635042/1‑148 (MQ=255)
         ggTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGTACTTGGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTGCTTTAAAAATTCGGATATG‑TGATAGATATAGGCTGAACACCa                                                                          <  2:635170/150‑1 (MQ=255)
          gTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGGTCTTTAAAAATTCGGATATG‑TGATAGATATAGGCTGAACACCAg                                                                         >  2:2218299/1‑150 (MQ=255)
           ttCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTG‑AGCTCGAATGTTGATTTATGGCTTTTGTCAGATCTTTCTTTAAAAATTCGGATCTG‑TGATAGAAATAAGCTGCccaccatt                                                                        >  2:1315156/1‑148 (MQ=11)
           ttCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGACTGAACACCAGt                                                                        >  2:2989959/1‑150 (MQ=39)
             cGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGACTGAACACCAGttt                                                                      <  2:1402554/150‑1 (MQ=38)
             cGATCACGCTTGGTTCCACCACTTTCTCCGTACGCAGTTACTTGTCAGCCCATCTGATGAAACATTC‑AGCGCTAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATATATATAGGCTGAACACCAGttt                                                                      <  1:1896945/150‑1 (MQ=11)
                 cccGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGACTGAACACCAGTTTCAct                                                                  <  2:1137533/150‑1 (MQ=38)
                    gcgtGGCTCCACCACGTTCGCCGTACGCAGTTACTTGTTAGACCTTAGAAATGAATCCTT‑AGCTCTAATGTTGATTTCTGGCTTTTGTCCGATCGTTCTTTAACAATTCGGATATG‑TGATAGATATAGGCTGAACACCAGTTTCActgct                                                               <  1:1100807/147‑1 (MQ=11)
                      ttGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGACTGAACACCAGt                                                                        >  2:2765502/1‑139 (MQ=40)
                      ttGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGACTGAACACCAGt                                                                        <  1:2765502/139‑1 (MQ=38)
                      ttGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGACTGAACACCAGTTTCACTGCtgg                                                             <  2:2713468/150‑1 (MQ=38)
                       tGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGGCTGAACACCAGTTTCACTGCtggt                                                            <  1:22800/150‑1 (MQ=255)
                         gCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGACTGAACACCAGttt                                                                      <  1:1639693/138‑1 (MQ=40)
                         gCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGACTGAACACCAGttt                                                                      >  2:1639693/1‑138 (MQ=39)
                          cTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGa                                                         >  1:1259306/1‑150 (MQ=39)
                           tCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGAt                                                        >  1:1348447/1‑150 (MQ=38)
                           tCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGAt                                                        >  1:2552921/1‑150 (MQ=39)
                            ccaccaCTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATc                                                       <  1:2182775/150‑1 (MQ=39)
                             caccacTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCa                                                      <  1:2723841/150‑1 (MQ=255)
                             caccacTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGACTGAACACCAGTTTCa                                                                    <  1:2632935/136‑1 (MQ=37)
                             caccacTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCa                                                      >  1:2943868/1‑150 (MQ=39)
                              accacTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGAt                                                        <  1:2527035/147‑1 (MQ=38)
                              accacTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGAt                                                        >  2:2527035/1‑147 (MQ=255)
                               ccacTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAgg                                                    <  1:2754705/150‑1 (MQ=39)
                                 acTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCt                                                  >  1:654425/1‑150 (MQ=255)
                                   tttCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGCACG‑TAAATCCACATTCGTTTGGGATGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTaa                                                >  1:362386/1‑150 (MQ=11)
                                   tttCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGGCTGAac                                                                              <  1:2467952/120‑1 (MQ=255)
                                   tttCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGGCTGAac                                                                              >  2:2467952/1‑120 (MQ=255)
                                   tttCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTaa                                                >  2:2744952/1‑150 (MQ=38)
                                    ttCGCCGTACGCATTTAGTTGTCAGAACTTAGAAATGAACATTC‑ATCTCGAATTTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAATAATTCGGATATG‑TGATAGATATAGGCTGAAAACCAGTTTCACTGCTGGTGGATCAGGCTAAg                                               <  2:1262123/150‑1 (MQ=11)
                                    ttCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGGCTGAACACCAGTTTCACTGCTTGTGGATCAGGCTAAg                                               <  2:588481/150‑1 (MQ=255)
                                    ttCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAg                                               >  1:844034/1‑150 (MQ=255)
                                    ttCGCCGTACGCAGTGACTTGTTAGAACTTATAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAg                                               <  2:955012/150‑1 (MQ=255)
                                             cGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAAttt                                      <  2:2668465/150‑1 (MQ=255)
                                             cGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGACTGAacac                                                                            <  2:2697879/112‑1 (MQ=40)
                                             cGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGACTGAacac                                                                            >  1:2697879/1‑112 (MQ=40)
                                              tcAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAg                                               <  1:21739/139‑1 (MQ=255)
                                              tcAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAg                                               >  2:21739/2‑140 (MQ=255)
                                              gCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTtg                                     >  2:1864768/1‑150 (MQ=38)
                                               cAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTtgt                                    >  2:957358/1‑150 (MQ=38)
                                               cAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTtgt                                    <  1:706462/150‑1 (MQ=255)
                                                aGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGACt                                                                                  <  1:300565/103‑1 (MQ=255)
                                                aGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGACt                                                                                  >  2:300565/1‑103 (MQ=255)
                                                  ttACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAg                                 >  2:3004174/1‑150 (MQ=37)
                                                    aCTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTtgt                                    >  2:1664023/1‑145 (MQ=25)
                                                    aCTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTtgt                                    <  1:1664023/145‑1 (MQ=25)
                                                      ttGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCt                             >  2:1981644/1‑150 (MQ=35)
                                                      ttGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTAGGATATG‑TGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCt                             >  1:2366957/1‑150 (MQ=25)
                                                       tGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTg                            <  2:2526270/150‑1 (MQ=25)
                                                        ttCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGc                           <  2:332079/149‑1 (MQ=21)
                                                        gTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGc                           <  2:1451967/150‑1 (MQ=21)
                                                         tCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTCCGGTAACCTTTGTGAGTTCTGcc                          >  2:1684914/1‑149 (MQ=11)
                                                         tCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCt                          >  2:2973103/1‑150 (MQ=25)
                                                         tCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGACTGAACACCAGTTTCActgct                                                               >  1:958478/1‑113 (MQ=38)
                                                         tCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCt                          >  2:1657108/1‑150 (MQ=35)
                                                          cAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTc                         <  1:662936/150‑1 (MQ=21)
                                                             aaCTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGaa                      >  2:721209/1‑150 (MQ=25)
                                                             aaCTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGaa                      <  2:601249/150‑1 (MQ=25)
                                                               cTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGACt                                                                                  >  2:1216509/1‑88 (MQ=40)
                                                               cTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGACt                                                                                  <  1:1216509/88‑1 (MQ=38)
                                                               cTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATGTGTGAGTTCTGCTCGAAag                    <  2:1810267/150‑1 (MQ=21)
                                                               cTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGACTGAACACCAGTTTCACTGCTAGTGGATCAGGCTAAGTTAAAAGTTGTGAGTTCTCCTCGaaaa                    >  1:1996076/1‑149 (MQ=255)
                                                                ttAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTtgt                                    <  2:2024688/133‑1 (MQ=37)
                                                                ttAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAaga                   <  2:210618/150‑1 (MQ=25)
                                                                ttAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGACt                                                                                  <  2:1579438/87‑1 (MQ=40)
                                                                ttAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGACt                                                                                  >  1:1579438/1‑87 (MQ=40)
                                                                ttAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCgagaga                   >  1:2049758/1‑150 (MQ=25)
                                                                ttAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAaga                   >  1:507056/1‑150 (MQ=35)
                                                                ttAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGAACGTTATTTAAAAATTCGGATATG‑TGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAaga                   <  2:2254559/150‑1 (MQ=255)
                                                                 tAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTctttcgag                  <  2:1707026/150‑8 (MQ=255)
                                                                 tAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAagag                  <  2:507056/150‑1 (MQ=35)
                                                                 tAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAagag                  <  1:2989959/150‑1 (MQ=35)
                                                                  aGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTtgt                                    <  2:1676050/131‑1 (MQ=37)
                                                                  aGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTtgt                                    >  1:1676050/1‑131 (MQ=37)
                                                                    aaaTGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCaa               <  2:2484414/150‑1 (MQ=25)
                                                                      atcaaCATTCGAGCT‑GAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACg             >  2:757466/4‑150 (MQ=11)
                                                                      aTGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACg             >  2:2328442/1‑150 (MQ=25)
                                                                       tcaaCATTCGAGCT‑GAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGt            <  1:757466/148‑1 (MQ=11)
                                                                        gAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTa           <  1:214285/150‑1 (MQ=25)
                                                                        gAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTa           <  2:1603471/150‑1 (MQ=25)
                                                                         aaCATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTAc          >  1:2951400/1‑150 (MQ=35)
                                                                            aTTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGaa       >  2:2172144/1‑150 (MQ=35)
                                                                                 aGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGACTGACCACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAAtttt   <  1:986280/150‑1 (MQ=17)
                                                                                  gCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGGCTGaa                                                                               >  1:1009382/1‑73 (MQ=17)
                                                                                  gCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTtgt                                    <  2:1413091/116‑1 (MQ=31)
                                                                                  gCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTc  <  1:1844362/150‑1 (MQ=31)
                                                                                  gCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTc  >  1:1794232/1‑150 (MQ=31)
                                                                                  gCTAGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGGCTGaa                                                                               <  2:1009382/73‑1 (MQ=9)
                                                                                   cTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGACTGAACACCAGTTTCACTGCtgg                                                             <  1:1329075/90‑1 (MQ=31)
                                                                                   cTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGACTGAACACCAGTTTCACTGCtgg                                                             >  2:1329075/1‑90 (MQ=31)
                                                                                   cTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAaga                   >  1:2980992/1‑132 (MQ=11)
                                                                                   cTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAaga                   <  2:2980992/132‑1 (MQ=11)
                                                                                                                                                      |                                                                                  
GAGGTCAGCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATG‑TGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTC  >  NC_002947/699577‑699807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A