Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F80 I0 R1 1105 125.4 6144758 94.6% 5812941 133.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,842,941 Δ1 bp 22.4% intergenic (+40/‑257) ccoP‑II → / → PP_4259 cbb3‑type cytochrome c oxidase subunit/iron‑sulfur cluster‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,842,9410C.22.4% 127.5 / 38.0 49intergenic (+40/‑257)ccoP‑II/PP_4259cbb3‑type cytochrome c oxidase subunit/iron‑sulfur cluster‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (17/21);  new base . (6/5);  total (23/26)
Fisher's exact test for biased strand distribution p-value = 7.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

GGGGCAGATGCCGGCCCAGGAACAACTGCAGGGCAACGACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGACTAAAGGTCGCAGTGCGAACCC  >  NC_002947/4842793‑4843088
                                                                                                                                                    |                                                                                                                                                    
ttggCAGATGCAGGCCCAGGAAAAACTGCCGGGCAAAGACAAGGTGCACTTGCTGGAGGTTTAGGTCTACTGCCTGTCGCATCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTAcaca                                                                                                                                                     <  2:2941173/148‑1 (MQ=255)
ggggCAGATGCCGGCCCAGGAACAACTGCAGGGCAACGACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTAcaca                                                                                                                                                     <  1:2866036/150‑1 (MQ=255)
ggggCAGATGCCGGCCCAGGAACAACTGCACGGCAACGACAAGTTGCACTTGCTGGCTGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTAcaca                                                                                                                                                     <  2:337902/150‑1 (MQ=255)
      gATGCCGGCCCAGGAACAACTGCAGGGCAACGACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGCGTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑AGGATCCt                                                                                                                                              <  2:221547/150‑1 (MQ=255)
        tGCCGGCCCAGGAACAACTGCAGGGCAACGACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCt‑t                                                                                                                                            <  1:1297100/150‑1 (MQ=255)
          ccGGCCCAGGAACAACTGCAGGGCAACGACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGt                                                                                                                                          <  2:171751/150‑1 (MQ=255)
           cGGCCCAGGAACAACTGCAGGGCAACGACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTa                                                                                                                                         <  2:255676/150‑1 (MQ=255)
            ggCCCAGGAACAACTGCAGGGCAACGACAAGGTGCACTTGCTGGCGGCCTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAg                                                                                                                                        <  2:2056887/150‑1 (MQ=255)
                  ggAACAACTGCAGGGCAACGACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGCCTTTTCCACCAGACGAAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑AGGATCCTGTGTAGGAGCgg                                                                                                                                  <  1:1135845/150‑1 (MQ=37)
                  ggAACAACTGCAGGGCAACGACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCgg                                                                                                                                  <  2:323026/150‑1 (MQ=255)
                  ggAACAACTGCAGGGCAACGACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑AGGATCCTGTGTAGGAGCgg                                                                                                                                  <  2:1454964/150‑1 (MQ=255)
                   gAACAACTGCAGGGCAACGACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCggg                                                                                                                                 <  2:2123682/150‑1 (MQ=255)
                   gAACAACTGCAGGGCAACGACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCggg                                                                                                                                 <  1:907685/150‑1 (MQ=255)
                          tGCAGGGCAACGACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTcccc                                                                                                                          <  2:1328685/150‑1 (MQ=255)
                          tGCAGGGCAACGACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTAccc                                                                                                                          <  2:2175000/150‑1 (MQ=255)
                               ggCAACGACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAACGATGCCCTGGGTAGGCCTCGCTGGTTGGTGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGaa                                                                                                                     <  1:2676429/150‑1 (MQ=25)
                                gCAACGACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGCTCCT‑TGTAGGAGCGGGTTTACCCGCGAag                                                                                                                    >  1:118141/1‑150 (MQ=255)
                                           gTGCACTTGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGaa                                                                                                                     <  2:95719/138‑1 (MQ=255)
                                           gTGCACTTGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGaa                                                                                                                     >  1:95719/1‑138 (MQ=255)
                                                 ttGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGccg                                                                                                                                  <  2:1946876/119‑3 (MQ=255)
                                                 ttGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGccg                                                                                                                                  >  1:1946876/1‑117 (MQ=255)
                                                 ttGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGc                                                                                                                                    >  1:1502188/1‑117 (MQ=255)
                                                 ttGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGc                                                                                                                                    <  2:1502188/117‑1 (MQ=255)
                                                     tGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAg                                                                                                                                        <  1:1201292/109‑1 (MQ=255)
                                                     tGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAg                                                                                                                                        >  2:1201292/1‑109 (MQ=255)
                                                     tGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTAc                                                                                                                            <  2:322155/121‑1 (MQ=255)
                                                     tGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTAc                                                                                                                            >  1:322155/1‑121 (MQ=255)
                                                            ttACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑AGGATCCTGTGTAGGAGc                                                                                                                                    >  2:2536404/1‑106 (MQ=25)
                                                            ttACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCCGGCCTCTTCGCGGGTAAACCCGCTCCTACA‑AGGATCCTGTGTAGGAGc                                                                                                                                    <  1:2536404/106‑1 (MQ=25)
                                                                   tACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGt                                                                                                                                <  2:3038799/103‑1 (MQ=255)
                                                                   tACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGt                                                                                                                                >  1:3038799/1‑103 (MQ=255)
                                                                                                                            ttCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAAtt                                                                  >  2:2577980/1‑108 (MQ=255)
                                                                                                                            ttCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAAtt                                                                  <  1:2577980/108‑1 (MQ=255)
                                                                                                                                  ggTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGACTAAAg                  >  2:1549225/1‑150 (MQ=255)
                                                                                                                                       aCCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTg                                                                                                        <  2:2598040/59‑1 (MQ=255)
                                                                                                                                       aCCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTg                                                                                                        >  1:2598040/1‑59 (MQ=255)
                                                                                                                                       aCCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGACTAAAGGTCGc             >  2:1752934/1‑150 (MQ=255)
                                                                                                                                         ccGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATaa                                                    >  2:1548677/1‑109 (MQ=255)
                                                                                                                                         ccGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATaa                                                    <  1:1548677/109‑1 (MQ=255)
                                                                                                                                         ccGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGACTAAAGGTCGCAg           >  1:693581/1‑150 (MQ=255)
                                                                                                                                         ccGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGACTAAAGGTCGCAg           >  2:2544275/1‑150 (MQ=255)
                                                                                                                                         ccGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGACTAAAGGTCGCAg           >  2:2935252/1‑150 (MQ=255)
                                                                                                                                         ccGCTCCTACA‑AGGATCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATAcc                                       <  1:1197766/122‑1 (MQ=255)
                                                                                                                                         ccGCTCCTACA‑AGGATCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATAcc                                       >  2:1197766/1‑122 (MQ=255)
                                                                                                                                         ccGCTCCTACA‑AGGATCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGACTAAAGGTCGCAg           >  1:2911687/1‑150 (MQ=255)
                                                                                                                                         ccGCTCCTACA‑AGGATCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGACTAAAGGTCGCAg           >  1:2549829/1‑150 (MQ=255)
                                                                                                                                           gCTCCTACA‑AGGATCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGACTAAAGGTCGCAGTg         >  1:2457296/1‑150 (MQ=255)
                                                                                                                                           gCTCCTACA‑AGGATCCTGTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGACTAAAGGTCGCAGTg         >  2:1451451/1‑150 (MQ=255)
                                                                                                                                               cTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCg                                                          <  1:1803389/97‑1 (MQ=255)
                                                                                                                                               cTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCg                                                          >  2:1803389/1‑97 (MQ=255)
                                                                                                                                                tACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCAttt                                                                                             >  2:1098026/1‑61 (MQ=255)
                                                                                                                                                tACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCAttt                                                                                             <  1:1098026/61‑1 (MQ=255)
                                                                                                                                                 acacAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGttt                                             <  2:2610742/108‑1 (MQ=255)
                                                                                                                                                 acacAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGttt                                             >  1:2610742/1‑108 (MQ=255)
                                                                                                                                                 acacAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGACTAAAGGTCGCAGTGCGAAcc   >  2:100049/1‑150 (MQ=255)
                                                                                                                                                  cacaGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGACTAAAGGTCGCAGTGCGAAccc  >  2:2094794/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GGGGCAGATGCCGGCCCAGGAACAACTGCAGGGCAACGACAAGGTGCACTTGCTGGCGGCTTACGTCTACAGCCTGTCGCACCAGGCTGAGCCGGCCAAGGCTGAGTAAGGCAGGCCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGATCCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACTGGCACAGCATTTCTCGCAGGCAAGTGACCTGGATCAATTGACACCCGCCATAACACGTTTCATACCACGAACCCAACGCGACTAAAGGTCGCAGTGCGAACCC  >  NC_002947/4842793‑4843088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A