Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F80 I0 R1
|
1105 |
125.4 |
6144758 |
94.6% |
5812941 |
133.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_002947 |
5,453,177 |
G→A |
27.7% |
intergenic (+142/‑213) |
lnt → / → PP_4791 |
apolipoprotein N‑acyltransferase/transposase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_002947 | 5,453,177 | 0 | G | A | 27.7%
| 46.8
/ 20.2
| 36 | intergenic (+142/‑213) | lnt/PP_4791 | apolipoprotein N‑acyltransferase/transposase |
| Reads supporting (aligned to +/- strand): ref base G (13/13); new base A (5/5); total (18/18) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GTCTGATTGACGATACGCGACCACTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCGGTTGGCCCGGTAGGGCCAGCCGCCTTTTGCTTTGTTAGGGTTCGTGTGCGCGGGACAGAGATCGTAGCCAGCACTGGTGTGTGTCTGCACACCCGCGTAGGAGAGTGATCCGCACGGTATCGGCGTGAAG > NC_002947/5453030‑5453288
|
gTCTGATTGACGATACGCGACCACTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCGGTTGGCCCGGTAGGGCCAgcc > 1:770806/1‑150 (MQ=255)
tCTGATTGACGATACGCGACCACTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCGGTTGGCCCGGTAGGGCCAgccg > 1:1511397/1‑150 (MQ=255)
tCTGATTGACGATACGCGACCACTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCGGTTGGCCCGGTAGGGCCAgccg > 2:1178265/1‑150 (MQ=255)
ttGACGATACGCGACCACTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCGGTTGGCCCGGTAGGGCCAGCCGCCttt > 2:439592/1‑150 (MQ=255)
ttGACGATACGCGACCACTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCGGTTGGCCCGGTAGGGCCAGCCGCCttt > 2:477757/1‑150 (MQ=255)
cGATACGCGACCACTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCGGTTGGCCCGGTAGGGCCAGCCGCCTTTTGCt < 2:543847/150‑1 (MQ=40)
aTACGCGACCACTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCGGTTGGCCCGGTAGGGCCAGCCGCCTTTTGCttt < 2:1685386/150‑1 (MQ=38)
tACGCGACCACTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCGGTTGGCCCGGTAGGGCCAGCCGCCTTTTGCTTTg < 1:2568887/150‑1 (MQ=38)
gcgACCACTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCGGTTGGCCCGGTAGGGCCAg > 2:2022977/1‑132 (MQ=35)
gcgACCACTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCGGTTGGCCCGGTAGGGCCAg < 1:2022977/132‑1 (MQ=35)
aCCACTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCGGTTGGCCCGGTAGGGCCAGCCGCCTTTTGCTTTGTTAggg < 1:2913268/150‑1 (MQ=32)
cACTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCGGTTGGCCCGGTAGGGCCAGCCGCCTTTTGCTTTGTTAGGGtt > 2:2666328/1‑150 (MQ=31)
cACTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCGGTTGGCCCGGTAGGGCCAGCCGCCTTTTGCTTTGTTAGGGtt > 2:2265045/1‑150 (MQ=31)
aCTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCGGTTGGCCCGGTAGGGCCAGCCGCCTTTTGCTTTGTTAGGGTTc < 2:641931/150‑1 (MQ=11)
aCTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCGGTTGGCCCGGTAGGGCCAGCCGCCTTTTGCTTTGTTAGGGTTc > 1:206178/1‑150 (MQ=11)
gcgcAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCGGCTGGCCCTACCGGGCCAACCGCCTTTTctttttcaaaatgtcagc < 1:627598‑M1/114‑19 (MQ=255)
gcgcAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCGGCTGGCCCTACCGGGCCAACCGCCTTTTctttttcaaaatgtcagc > 2:627598‑M1/1‑96 (MQ=255)
ggCGGTTGGCCCGGTAGGGCCAGCCGCCTTTTctttttcaaaatgtcagcgtcgctgcggaactgaagccgtacacctcatgtttgctgcgcgacagcgcagcccggaagggctgg > 2:1221705‑M1/1‑32 (MQ=255)
ggCGGTTGGCCCGGTAGGGCCAGCCGCCTTTTctttttcaaaatgtcagcgtcgctgcggaactgaagccgtacacctcatgtttgctgcgcgacagcgcagcccggaagggctgg < 1:1221705‑M1/116‑85 (MQ=255)
gCGGTTGGCCCGGTAGGGCCAGCCGCCTTTTctttttcaaaatgtcagcgtcgctgcggaactgaagccgtacacctcatgtttgctgcgcgacagcgcagcccggaagggctgggtgatctcctacggggat < 2:1582282‑M1/133‑103 (MQ=255)
gCGGTTGGCCCGGTAGGGCCAGCCGCCTTTTctttttcaaaatgtcagcgtcgctgcggaactgaagccgtacacctcatgtttgctgcgcgacagcgcagcccggaagggctgggtgatctcctacggggat > 1:1582282‑M1/1‑31 (MQ=255)
gCGGTTGGCCCGGTAGGGCCAGCCGCCTTTTctttttcaaaatgtcagcgtcgctgcggaactgaagccgtacacctcatgtttgctgcgcgacagcgcagcccggaagggctgggtgatctcctacgg < 2:1858300‑M1/129‑99 (MQ=255)
gCGGTTGGCCCGGTAGGGCCAGCCGCCTTTTctttttcaaaatgtcagcgtcgctgcggaactgaagccgtacacctcatgtttgctgcgcgacagcgcagcccggaagggctgggtgatctcctacgg > 1:1858300‑M1/1‑31 (MQ=255)
gCGGTTGGCCCGGTAGGGCCAGCCGCCTTTTctttttcaaaatgtcagcgtcgctgcggaactgaagccgtacacctcatgtttgctgcgcgacagcgcagcccggaagggctgggtgatctcctaca < 1:2818628‑M1/128‑98 (MQ=255)
gCGGTTGGCCCGGTAGGGCCAGCCGCCTTTTctttttcaaaatgtcagcgtcgctgcggaactgaagccgtacacctcatgtttgctgcgcgacagcgcagcccggaagggctgggtgatctcctaca > 2:2818628‑M1/1‑31 (MQ=255)
gCGGTTGGCCCGGTAGGGCCAGCCGCCTTTTctttttcaaaatgtcagcgtcgctgcggaactgaagccgtacacctcatgtttgctgcgcgacagcgcagcccggaagggctgggt > 1:2074665‑M1/1‑31 (MQ=255)
gCGGTTGGCCCGGTAGGGCCAGCCGCCTTTTctttttcaaaatgtcagcgtcgctgcggaactgaagccgtacacctcatgtttgctgcgcgacagcgcagcccggaagggctgggt < 2:2074665‑M1/117‑87 (MQ=255)
cGGTTGGCCCGGTAGGGCCAGCCGCCTTTTctttttcaaaatgtcagcgtcgctgcggaactgaagccgtacacctcatgtttgctgcg < 1:2918261‑M1/89‑60 (MQ=255)
cGGTTGGCCCGGTAGGGCCAGCCGCCTTTTctttttcaaaatgtcagcgtcgctgcggaactgaagccgtacacctcatgtttgctgcg > 2:2918261‑M1/1‑30 (MQ=255)
ggTTGGCCCGGTAGGGCCAACCGCCTTTTctttttcaaaatgtcagcgtcgctgcggaactgaagccgtacacctcatgtttgctgcgcgacagcgca > 1:2043326‑M1/1‑29 (MQ=255)
ggTTGGCCCGGTAGGGCCAACCGCCTTTTctttttcaaaatgtcagcgtcgctgcggaactgaagccgtacacctcatgtttgctgcgcgacagcgca < 2:2043326‑M1/98‑70 (MQ=255)
ggTTGGCCCGGTAGGGCCAACCGCCTTTTctttttcaaaatgtcagcgtcgctgcggaactgaagccgtacacctcat < 1:1341690‑M1/78‑50 (MQ=255)
ggTTGGCCCGGTAGGGCCAACCGCCTTTTctttttcaaaatgtcagcgtcgctgcggaactgaagccgtacacctcat > 2:1341690‑M1/1‑29 (MQ=255)
gTTGGCCCGGTAGGGCCAGCCGCCTTTTctttttcaaaatgtcagcgtcgctgcggaactgaagccgtacacctcatgtttgctgcgcgacagcgcagcccggaagggctgggtgatc > 2:2552080‑M1/1‑28 (MQ=255)
gTTGGCCCGGTAGGGCCAGCCGCCTTTTctttttcaaaatgtcagcgtcgctgcggaactgaagccgtacacctcatgtttgctgcgcgacagcgcagcccggaagggctgggtgatc < 1:2552080‑M1/118‑91 (MQ=255)
ttGGCCCGGTAGGGCCAGCCGCCTTTTctttttcaaaatgtcagcgtcgctgcggaactgaagccgtacacctcatgtttgctgcgcgacagcgcagcccggaagggctgggtgat < 2:2780661‑M1/116‑90 (MQ=255)
ttGGCCCGGTAGGGCCAGCCGCCTTTTctttttcaaaatgtcagcgtcgctgcggaactgaagccgtacacctcatgtttgctgcgcgacagcgcagcccggaagggctgggtgat > 1:2780661‑M1/1‑27 (MQ=255)
ttGGCCCGGTAGGGCCAACCGCCTTTTctttttcaaaatgtcagcgtcgctgcggaactgaagccgtacacctcatgtttgctgcgcg > 2:1489535‑M1/1‑27 (MQ=255)
ttGGCCCGGTAGGGCCAACCGCCTTTTctttttcaaaatgtcagcgtcgctgcggaactgaagccgtacacctcatgtttgctgcgcg < 1:1489535‑M1/88‑62 (MQ=255)
ggTAGGGCCAACCGCCTTTTctttttcaaaatgtcagcgtcgctgcggaactgaagccgtacac < 1:991784‑M1/64‑45 (MQ=255)
ggTAGGGCCAACCGCCTTTTctttttcaaaatgtcagcgtcgctgcggaactgaagccgtacac > 2:991784‑M1/1‑20 (MQ=255)
|
GTCTGATTGACGATACGCGACCACTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCGGTTGGCCCGGTAGGGCCAGCCGCCTTTTGCTTTGTTAGGGTTCGTGTGCGCGGGACAGAGATCGTAGCCAGCACTGGTGTGTGTCTGCACACCCGCGTAGGAGAGTGATCCGCACGGTATCGGCGTGAAG > NC_002947/5453030‑5453288
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
