Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F83 I0 R1
|
969 |
96.3 |
4677704 |
94.6% |
4425107 |
134.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_002947 |
955,155 |
T→A |
20.9% |
intergenic (+106/‑207) |
cyoE → / → alaC |
protoheme IX farnesyltransferase/aminotransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_002947 | 955,155 | 0 | T | A | 20.9%
| 106.6
/ 19.2
| 47 | intergenic (+106/‑207) | cyoE/alaC | protoheme IX farnesyltransferase/aminotransferase |
| Reads supporting (aligned to +/- strand): ref base T (20/17); new base A (6/4); total (26/21) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCT > NC_002947/955016‑955290
|
cGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTAGGa < 1:645400/150‑1 (MQ=255)
cGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGAACCT‑TGTAGGAGCggg < 1:229824/150‑1 (MQ=37)
ggATGTGCTGATGACTTACGCACGCTGAAACTGCTGCCTGTTTCACGAACACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGCGGGt < 1:981969/150‑1 (MQ=255)
aTGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTa < 2:2213358/139‑1 (MQ=255)
aTGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTa > 1:2213358/1‑139 (MQ=255)
tgatgaCCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGAACCT‑TGTAGGAGCGGGTTTACCcgc > 2:1157984/1‑150 (MQ=37)
tgaCCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTAC‑AGGAACCTTGTAGGAGCGGGTTTACCCGCGaa < 1:1157984/150‑1 (MQ=25)
cTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGt > 1:1546805/1‑125 (MQ=255)
cTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGt < 2:1546805/125‑1 (MQ=255)
tACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGCgg > 2:394219/1‑132 (MQ=255)
tACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGCgg < 1:394219/132‑1 (MQ=255)
gcacgcTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGAACCT‑TGTAGGAGCgg > 2:2141530/1‑129 (MQ=25)
cTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGCGGGt > 2:718947/1‑127 (MQ=255)
cTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGCGGGt < 1:718947/127‑1 (MQ=255)
cTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAg > 1:463698/1‑150 (MQ=255)
tgctgcCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGAACCT‑TGTAGGAGCggg > 2:1343629/1‑118 (MQ=25)
tgctgcCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTAC‑AGGAACCTTGTAGGAGCggg < 1:1343629/118‑1 (MQ=25)
tgcCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGaaaaataaaa < 2:463698/150‑1 (MQ=255)
ccTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGCgg < 1:1665262/112‑1 (MQ=255)
ccTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGCgg > 2:1665262/1‑112 (MQ=255)
cGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTATTACTGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGCGGGGTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGAttt > 1:1918220/1‑150 (MQ=38)
cccGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGc > 2:629269/1‑114 (MQ=255)
cccGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGc < 1:629269/114‑1 (MQ=255)
ccGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑Tg > 2:2271086/1‑86 (MQ=255)
ccGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑Tg < 1:2271086/86‑1 (MQ=255)
ccGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAaga < 1:745957/110‑1 (MQ=255)
ccGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAaga > 2:745957/1‑110 (MQ=255)
ccGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCaaaaaa > 1:327087/1‑150 (MQ=255)
gTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGaaa < 2:327087/150‑1 (MQ=255)
gtgCCGGCTTCCTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTAc < 2:1918220/150‑1 (MQ=255)
gCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACaa > 2:108243/1‑149 (MQ=255)
ttCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGAt < 1:1084614/102‑1 (MQ=255)
ttCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAAGAGAt > 2:1084614/1‑102 (MQ=255)
gggTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTAc < 1:1634651/134‑1 (MQ=255)
gggTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTAc > 2:1634651/1‑134 (MQ=255)
cccGCTCCTAC‑AGGAACCTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtc > 2:1658615/1‑150 (MQ=37)
cccGCTCCTAC‑AGGAACCTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtc > 1:1473133/1‑150 (MQ=37)
ccGCTCCTACAGGAACCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAAtt > 2:1625279/1‑117 (MQ=25)
ccGCTCCTACAGGAACCT‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAAtt < 1:1625279/117‑1 (MQ=25)
ccGCTCCTACAAGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtct > 2:1987084/1‑150 (MQ=255)
ccGCTCCTACAAGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtct > 1:497872/1‑150 (MQ=255)
ccGCTCCTACAAGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtct > 2:1179136/1‑150 (MQ=255)
ccGCTCCTACAAGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtct > 1:1789683/1‑150 (MQ=255)
aCAAGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTc < 2:1172793/92‑1 (MQ=255)
aCAAGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTc > 1:1172793/1‑92 (MQ=255)
aaGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAg > 1:876665/1‑118 (MQ=255)
aaGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAg < 2:876665/118‑1 (MQ=255)
|
CGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCC‑TGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCT > NC_002947/955016‑955290
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
