Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F83 I0 R1 969 96.3 4677704 94.6% 4425107 134.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,648,917 Δ1 bp 100% intergenic (‑154/+142) oprB‑II ← / ← PP_1446 carbohydrate‑selective porin/TonB‑dependent receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,648,9170G.97.0% 397.3 / 1.7 92intergenic (‑154/+142)oprB‑II/PP_1446carbohydrate‑selective porin/TonB‑dependent receptor
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base . (50/39);  total (52/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TCTTTTGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACACCGATCTTCGTCGCTTGTGGTTCA  >  NC_002947/1648776‑1649061
                                                                                                                                              |                                                                                                                                                  
tCTTTTGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGAtt                                                                                                                                           >  2:2068995/1‑150 (MQ=255)
  ttttGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAGAACAAATTGTC‑CAGAGGAttgt                                                                                                                                         >  2:1189420/1‑150 (MQ=255)
   tttGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGAttgtt                                                                                                                                        <  2:794040/150‑1 (MQ=255)
      gATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGAttgttgtt                                                                                                                                     <  1:2068995/150‑1 (MQ=255)
      gATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGAttgttgtt                                                                                                                                     >  1:1799586/1‑150 (MQ=255)
        ttattGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGa                                                                                                                                   >  1:1327795/1‑150 (MQ=255)
         tattGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGaa                                                                                                                                  >  1:1132316/1‑150 (MQ=255)
             caaaaaaaCGTCACGAGCATCGAAGGGCGGGCCCCTGAATGGATATGGCGACAAGATTGTTGGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGTAAGTTAGAAGAAAAAAGACAAATTGTC‑CACTGGATTGTGGTTGAACggg                                                                                                                              <  2:558123/149‑1 (MQ=255)
              aaaaaaaCGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGc                                                                                                                             <  2:699893/150‑1 (MQ=255)
                aaaaaCGGCAAGCGCATCGAAGGGCGGGCCCCTCACTGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATAGGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGc                                                                                                                             <  1:713803/150‑1 (MQ=255)
                 aaaaCGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAAc                                                                                                                          >  1:2336020/1‑150 (MQ=255)
                    aCGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAgt                                                                                                                       >  1:1338113/1‑150 (MQ=255)
                         aaGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGACAACTGAGGCGTGTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCa                                                                                                                  >  2:1736330/1‑150 (MQ=255)
                           gcgcATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGAGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCAtt                                                                                                                >  2:755157/1‑150 (MQ=255)
                              cATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAAt                                                                                                             >  1:339621/1‑150 (MQ=255)
                                 cGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑Ga                                                                                                        <  2:2331516/150‑1 (MQ=255)
                                 cGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGCC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑Ga                                                                                                        <  1:260897/150‑1 (MQ=255)
                                   aaGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAgt                                                                                                      >  2:721033/1‑149 (MQ=255)
                                   aaGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAgg                                                                                                      >  2:2306264/1‑150 (MQ=255)
                                   aaGGGCGGGCCCCCAAATGGATATGGCGAGAAGATTGTCAGAGGAAGCTTTTCAGCTTGCGGCGGGGAGGATATA‑TGGGAAGTTATAACAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAgg                                                                                                      <  1:22058/150‑1 (MQ=255)
                                      ggcgggcCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACg                                                                                                                                <  1:1854487/123‑1 (MQ=255)
                                      ggcgggcCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACg                                                                                                                                >  2:1854487/1‑123 (MQ=255)
                                      ggcgggcCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAg                                                                                                   >  2:824104/1‑150 (MQ=255)
                                      ggcgggcCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAg                                                                                                   >  2:1203523/1‑150 (MQ=255)
                                          ggcCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGgtgt                                                                                               <  1:2306264/150‑1 (MQ=255)
                                          ggcCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGgtgt                                                                                               <  1:2296313/150‑1 (MQ=255)
                                             cccTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGc                                                                                                                             <  2:1013009/119‑1 (MQ=255)
                                             cccTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGc                                                                                                                             >  1:1013009/1‑119 (MQ=255)
                                             cccTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAgg                                                                                            >  1:1762571/1‑150 (MQ=255)
                                               cTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAACAAGACGGATGGGC‑CGGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑CAGGCAGGTGTAGGAg                                                                                          >  2:72150/1‑150 (MQ=255)
                                               cTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACgg                                                                                                                               <  2:1203866/115‑1 (MQ=255)
                                               cTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACgg                                                                                                                               >  1:1203866/1‑115 (MQ=255)
                                                tcggaggATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGT‑ACAGAGGATTGTTGTTGAACGGGCCACAGTCGGCATTAATC‑‑GAGGCAGGTGTAGGAGc                                                                                         >  1:672040/6‑150 (MQ=255)
                                                tCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGgtgt                                                                                               >  1:2004037/1‑144 (MQ=255)
                                                tCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGgtgt                                                                                               <  2:2004037/144‑1 (MQ=255)
                                                tCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAGGAGc                                                                                         <  1:1128505/150‑1 (MQ=255)
                                                tCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAGGAGc                                                                                         <  2:1762571/150‑1 (MQ=255)
                                                  aaTGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAGGAGCgg                                                                                       >  1:1281495/1‑150 (MQ=255)
                                                  aaTGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAGGAGCgg                                                                                       <  2:1327795/150‑1 (MQ=255)
                                                  aaTGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAGGAGCgg                                                                                       >  1:78724/1‑150 (MQ=255)
                                                   aTGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAGGAGCggg                                                                                      >  2:866001/1‑150 (MQ=255)
                                                    tGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTAGAAGGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAGGAGCGGGt                                                                                     >  1:2006769/1‑150 (MQ=255)
                                                        tatGGGGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAGGAGCGGGTTCAc                                                                                 >  2:271254/1‑150 (MQ=255)
                                                          tGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAGGAGCGGGTTCAccc                                                                               >  1:173991/1‑150 (MQ=255)
                                                           ggCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAgtcgtc                                                                                                                   >  1:1682152/1‑115 (MQ=255)
                                                           ggCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAgtcgtc                                                                                                                   <  2:1682152/115‑1 (MQ=255)
                                                           ggCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAGGAGCGGGTTCACCcg                                                                              >  1:1153533/1‑150 (MQ=255)
                                                            gCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAGGAGCGGGTTCACCcgc                                                                             <  2:2336020/150‑1 (MQ=255)
                                                            gCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAGGAGCGGGTTCACCcgc                                                                             >  1:439425/1‑150 (MQ=255)
                                                              gACAAGATTGTTCGATGAAGGGTTTCAGCTTGCGGCGGGGAGGATATAGGGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAGGAGCGGGTTCACCcgc                                                                             >  2:96785/1‑150 (MQ=255)
                                                                       gTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCgg                                                                  >  2:1383658/1‑150 (MQ=255)
                                                                        ttCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAGGAGCggg                                                                                      <  2:874965/129‑1 (MQ=255)
                                                                        ttCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAGGAGCggg                                                                                      >  1:874965/1‑129 (MQ=255)
                                                                        ttCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGt                                                                 <  2:78724/150‑1 (MQ=255)
                                                                           gATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGGCGTCATTAATC‑‑GAGGCAGGTGTAGGAGCGGGTGCCACCGCGACTGCGCCGGGTga                                                              >  2:1731864/1‑149 (MQ=255)
                                                                              gAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCt                                                           >  1:1761497/1‑150 (MQ=255)
                                                                                aGCGTTTCAGCTTACGGCGGGGAGGAAATG‑GGGGAAGTGATAAGAAAAAAGACAAATTGGC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTAc                                                         >  2:89338/1‑150 (MQ=255)
                                                                                 gCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTGGAATGGCATCCAG‑CGT‑GTTGCTCAGGCGGTACGGGTAGGAGCGGGTTCACCCGCGAATGCGCTGGGGGCCCTAcc                                                        >  1:2026671/1‑150 (MQ=255)
                                                                                    tttCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATc                                                     <  2:1992286/150‑1 (MQ=255)
                                                                                        aGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTAt                                                 <  2:339621/150‑1 (MQ=255)
                                                                                          cTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTc                                               >  1:446781/1‑150 (MQ=255)
                                                                                           ttGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAGGAGc                                                                                         <  1:2097390/107‑1 (MQ=255)
                                                                                           ttGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAGGAGc                                                                                         >  2:2097390/1‑107 (MQ=255)
                                                                                              cggcggGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCgg                                           <  1:1580048/150‑1 (MQ=255)
                                                                                                     gaggaTATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAAcc                                    >  1:996927/1‑150 (MQ=255)
                                                                                                     gaggaTATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAAcc                                    >  2:2104266/1‑150 (MQ=255)
                                                                                                     gaggaTATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAAcc                                    >  2:406824/1‑150 (MQ=255)
                                                                                                        gaTATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGt                                 <  2:1281495/150‑1 (MQ=255)
                                                                                                         atatA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGtt                                <  2:1799586/150‑1 (MQ=255)
                                                                                                          tata‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTc                               <  1:1736330/150‑1 (MQ=255)
                                                                                                          tata‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTc                               <  2:238733/150‑1 (MQ=255)
                                                                                                          tata‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTc                               >  2:433131/1‑150 (MQ=255)
                                                                                                           ata‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTcc                              <  2:1761497/150‑1 (MQ=255)
                                                                                                             a‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTc                               >  2:765823/1‑147 (MQ=255)
                                                                                                             a‑GGGGAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTc                               <  1:765823/147‑1 (MQ=255)
                                                                                                                 ggAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTacac                         <  1:1122793/150‑1 (MQ=255)
                                                                                                                  gAAGTTATAAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAGGAGCGGGGTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCATTCCTACAcc                        >  2:1886515/1‑150 (MQ=255)
                                                                                                                       tataAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAgtc                                                                                                                      <  2:1961291/53‑1 (MQ=255)
                                                                                                                       tataAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAgtc                                                                                                                      >  1:1961291/1‑53 (MQ=255)
                                                                                                                         taAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAGGAGCGGGTTCACCCGCGTTAGACGCGGTGGGGCTACCATCGTATTCGCGGGTGAACCCGTGCCTACACCGATCTtc                 <  2:1153533/150‑1 (MQ=255)
                                                                                                                         taAGAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACACCGATCTtc                 >  2:2174096/1‑150 (MQ=255)
                                                                                                                         aaaGAAAAAAGACAACTTGTC‑TAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GCGGCGGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACACCGATCTtc                 <  2:439425/149‑1 (MQ=39)
                                                                                                                            gAAAAAAGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACACCGATCTtcgtc              >  2:203148/1‑150 (MQ=255)
                                                                                                                                aaaGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACACCGATCTTCGTCGCtt          <  1:824104/150‑1 (MQ=255)
                                                                                                                                aaaGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACACCGATCTTCGTCGCtt          <  1:1203523/150‑1 (MQ=255)
                                                                                                                                  aGACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACACCGATCTTCGTCGCTtgt        >  1:791598/1‑150 (MQ=255)
                                                                                                                                   gACAAATTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACACCGATCTTCGTCGCTtgtg       >  1:206882/1‑150 (MQ=255)
                                                                                                                                      aaaTTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACACCGATCTtc                 <  1:2015575/137‑1 (MQ=255)
                                                                                                                                      aaaTTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACACCGATCTtc                 >  2:2015575/1‑137 (MQ=255)
                                                                                                                                      aaaTTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACACCGATCTTCGTCGCTTGTGGt     >  2:817419/1‑149 (MQ=255)
                                                                                                                                      aaaTTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACACCGATCTTCGTCGCTTGTGGt     <  1:817419/149‑1 (MQ=255)
                                                                                                                                        aTTGTC‑CAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACACCGATCTTCGTCGCTTGTGGTTCa  <  1:203148/150‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
TCTTTTGATTATTGAAAAAAACGGCAAGCGCATCGAAGGGCGGGCCCCTCAATGGATATGGCGACAAGATTGTTCGATGAAGCGTTTCAGCTTGCGGCGGGGAGGATATA‑GGGGAAGTTATAAGAAAAAAGACAAATTGTCGCAGAGGATTGTTGTTGAACGGGCAACAGTCGTCATTAATC‑‑GAGGCAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACACCGATCTTCGTCGCTTGTGGTTCA  >  NC_002947/1648776‑1649061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A