Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F91 I0 R1	918	53.4	2573728	94.2%	2424451	136.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,327,127	C→T	55.4%	intergenic (+97/‑196)	PP_16SE → / → PP_23SE	16S ribosomal RNA/23S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,327,127	0	C	T	55.4%	1.1 / 86.8	74	intergenic (+97/‑196)	PP_16SE/PP_23SE	16S ribosomal RNA/23S ribosomal RNA
Reads supporting (aligned to +/- strand): ref base C (15/18); new base T (26/15); total (41/33)
Fisher's exact test for biased strand distribution p-value = 1.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.71e-01

TCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGT  >  NC_002947/1326983‑1327233

tCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACAACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGa                                                                                                       >  1:268870/1‑150 (MQ=17)

   tAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATg                                                                                                    <  1:321946/150‑1 (MQ=17)

        aGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGAt                                                                                               >  2:735853/1‑150 (MQ=32)

            aGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCt                                                                                           >  1:510482/1‑150 (MQ=17)

             gCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTg                                                                                          <  2:956037/150‑1 (MQ=17)

                gTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCt                                                                                       >  2:696059/1‑150 (MQ=17)

                   ggggAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCtttt                                                                                    <  1:735853/150‑1 (MQ=32)

                    gggAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTg                                                                                   <  1:632494/150‑1 (MQ=32)

                         ccTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGa                                                                              <  1:1239704/150‑1 (MQ=32)

                          cTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGAt                                                                             >  2:317740/1‑150 (MQ=17)

                               gCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTc                                                                        <  2:1083761/150‑1 (MQ=32)

                                   gATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTa                                                                    >  2:1254587/1‑150 (MQ=17)

                                     tCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTaaa                                                                  >  1:424389/1‑150 (MQ=17)

                                       aCCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTaaaaa                                                                >  2:1022025/1‑150 (MQ=17)

                                         ctcctTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGtt                                                                         >  1:1024313/1‑139 (MQ=18)

                                         ctcctTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGtt                                                                         <  2:1024313/139‑1 (MQ=18)

                                          tcctTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGa                                                                              <  2:821094/133‑1 (MQ=33)

                                          tcctTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGa                                                                              >  1:821094/1‑133 (MQ=33)

                                            ctTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCgg                                                           >  2:687672/1‑150 (MQ=32)

                                             ttAATCGACGACATCAGGCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGa                                                          <  2:420084/150‑1 (MQ=17)

                                             ttAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGa                                                          >  1:387850/1‑150 (MQ=17)

                                             ttAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGa                                                          >  1:961631/1‑150 (MQ=17)

                                              tAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGat                                                         <  2:96884/150‑1 (MQ=32)

                                                 tCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATAtg                                                      >  2:1064385/1‑150 (MQ=17)

                                                  cgacgaCATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATAtgt                                                     <  2:1169911/150‑1 (MQ=17)

                                                    acgacATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTga                                                   >  2:1143999/1‑150 (MQ=17)

                                                     gctcATCAGCAGGCTGATGGGCTCCCACACGAATTGCTTGATTCATTGTCGCAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTgat                                                  >  1:801872/4‑150 (MQ=11)

                                                      gacATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTgata                                                 <  2:1123930/150‑1 (MQ=17)

                                                      gacATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTgata                                                 <  2:430787/150‑1 (MQ=32)

                                                            aGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGa                                                          >  1:183441/1‑135 (MQ=18)

                                                            aGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGa                                                          <  2:183441/135‑1 (MQ=17)

                                                              ccTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGa                                         <  2:850463/150‑1 (MQ=32)

                                                              ccTGCTGATGAGCCCCCACACGAATTGCTTGATTCATTGGCGAAGACGTTTTTCCTGGTCAGAACCTGTGAATGTGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGa                                         <  1:862791/150‑1 (MQ=11)

                                                                tgctgATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATCAGTGATATATATAGACt                                       >  2:996726/1‑150 (MQ=255)

                                                                   tgatgaGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGaa                                    >  1:916052/1‑150 (MQ=32)

                                                                    gatgaGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAac                                   >  2:513114/1‑150 (MQ=32)

                                                                    gatgaGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCATGGTCAGAACCTGGAAATGAGCATTCGTATAGAATGTTGATTTCTGGCTTTTGTCAGATCGTTATTTAAAAATTCGGGTATGTGATAGATATAGACTGAac                                   >  1:579498/1‑150 (MQ=11)

                                                                     atgaGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTc                                                             <  1:1272257/123‑1 (MQ=33)

                                                                     atgaGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTc                                                             >  2:1272257/1‑123 (MQ=33)

                                                                     atgaGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTg                                      <  2:557208/146‑1 (MQ=32)

                                                                     atgaGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTg                                      >  1:557208/1‑146 (MQ=32)

                                                                      tgaGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAacac                                 <  2:58433/150‑1 (MQ=32)

                                                                          cTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGa                                         <  2:764051/138‑1 (MQ=18)

                                                                          cTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGa                                         >  1:764051/1‑138 (MQ=18)

                                                                           tCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACTTCAGtt                            >  2:1056874/1‑150 (MQ=12)

                                                                             ccACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTc                          >  2:872577/1‑150 (MQ=32)

                                                                              cacaCGAATTGCTTGATTCATTGTCGAAGTCGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCa                         <  1:1237278/150‑1 (MQ=17)

                                                                               acacGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTgata                                                 >  1:502799/1‑125 (MQ=18)

                                                                               acacGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTgata                                                 <  2:502799/125‑1 (MQ=18)

                                                                               acacGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTATGGCTTTTGCCAGATTGATTTTTAAAAGAGCTGATATGTGATAGATATAGACTGAATATCAGTTTCAc                        >  2:280872/1‑150 (MQ=11)

                                                                                      ttGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCtggt                 >  2:155662/1‑150 (MQ=17)

                                                                                      ttGCTTGATTAGTTGTAGAAGACGTATTTCCGGGGCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCtggt                 <  2:916052/150‑1 (MQ=11)

                                                                                        gCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATAtgt                                                     >  1:583615/1‑112 (MQ=33)

                                                                                        gCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATAtgt                                                     <  2:583615/112‑1 (MQ=33)

                                                                                         cTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCttt                                                                                     <  2:1192726/79‑1 (MQ=19)

                                                                                         cTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCttt                                                                                     >  1:1192726/1‑79 (MQ=25)

                                                                                         cTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGa              <  2:424389/150‑1 (MQ=17)

                                                                                         cTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGa              <  1:317740/150‑1 (MQ=17)

                                                                                         cTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGa              >  1:586692/1‑150 (MQ=32)

                                                                                          ttGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATAtgt                                                     <  2:422370/110‑1 (MQ=18)

                                                                                          ttGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATAtgt                                                     >  1:422370/1‑110 (MQ=18)

                                                                                            gATTCATTGTCGAAGACGTTTTTCTTTTTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCa           >  2:1249267/1‑150 (MQ=12)

                                                                                            gATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCa           <  2:387850/150‑1 (MQ=17)

                                                                                            gATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCa           >  1:527696/1‑150 (MQ=32)

                                                                                            gATTCATTGTCGAAGACGTTTCTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCa                         <  1:825930/136‑1 (MQ=12)

                                                                                            gATTCATTGTCGAAGACGTTTCTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCa                         >  2:825930/1‑136 (MQ=12)

                                                                                             aTTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAg          >  2:361309/1‑150 (MQ=17)

                                                                                               tCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTgg                                                                                         >  1:1167420/1‑69 (MQ=37)

                                                                                               tCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTgg                                                                                         <  2:1167420/69‑1 (MQ=35)

                                                                                                 aTTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTa      >  1:374472/1‑150 (MQ=17)

                                                                                                 aTTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATAtgt                                                     <  2:925207/103‑1 (MQ=33)

                                                                                                 aTTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATAtgt                                                     >  1:925207/1‑103 (MQ=33)

                                                                                                    gTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGAGATAGACTGAACTCCAGTTTCACTGCTGGTGGATCAGGCTAAgg   <  2:268870/150‑1 (MQ=12)

                                                                                                     tCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGt  >  1:509309/1‑150 (MQ=17)

TCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGT  >  NC_002947/1326983‑1327233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A