Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F91 I0 R1
|
918 |
53.4 |
2573728 |
94.2% |
2424451 |
136.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_002947 |
1,852,271 |
T→C |
23.7% |
Y439H (TAC→CAC) |
relA → |
ATP:GTP 3'‑pyrophosphotransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_002947 | 1,852,271 | 0 | T | C | 23.7%
| 91.7
/ 27.1
| 55 | Y439H (TAC→CAC) | relA | ATP:GTP 3'‑pyrophosphotransferase |
| Reads supporting (aligned to +/- strand): ref base T (22/20); new base C (7/6); total (29/26) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01 |
AGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCAGCCGTGACTGGTTGAACTC > NC_002947/1852122‑1852419
|
aGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTCCTTGGCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCt < 1:978962/150‑1 (MQ=255)
tGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTAcc < 1:659225/150‑1 (MQ=255)
tGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTAcc > 1:1286468/1‑150 (MQ=255)
ggAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGt < 1:969212/150‑1 (MQ=255)
gCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAATGCGCCACGCCATTGGACTTCGTCTACCGCGTGCacac > 2:527681/1‑150 (MQ=255)
gCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCCACCGCGTGCacac > 2:349838/1‑150 (MQ=255)
aCGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCg > 1:257224/1‑150 (MQ=255)
aCGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCg > 1:177929/1‑150 (MQ=255)
aCGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCCACCGCGTGCACACCg > 1:413461/1‑150 (MQ=255)
cTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGc < 2:132315/150‑1 (MQ=255)
cTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCCACCGCGTGCACACCGAGATCGGc < 1:131784/150‑1 (MQ=255)
gggCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCGCAGGCTCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCca < 1:732741/150‑1 (MQ=255)
aCATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACaatt < 2:1216293/150‑3 (MQ=255)
ctggccGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGcgcg > 1:62680/1‑150 (MQ=255)
cGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGAt > 1:737135/1‑150 (MQ=255)
gCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGAGCGAAGATCaa > 1:380131/1‑150 (MQ=255)
tGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGcc < 1:932991/150‑1 (MQ=255)
gTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCg < 2:117511/112‑1 (MQ=255)
gTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCg > 1:117511/1‑112 (MQ=255)
cATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGcc > 1:524742/1‑150 (MQ=255)
aTCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCCACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCg > 2:650507/1‑150 (MQ=255)
ccTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCCACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAAc < 1:575957/150‑1 (MQ=255)
cGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCCACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGc < 1:1193519/150‑1 (MQ=255)
ttATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCa < 2:62680/150‑1 (MQ=255)
tgtgTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGAc < 2:257224/150‑1 (MQ=255)
tgtTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTg > 2:239214/1‑150 (MQ=255)
ttCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGc < 2:380131/150‑1 (MQ=255)
ttCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCCACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGc < 1:650507/150‑1 (MQ=255)
cccccGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGCAGAGCAACGGCCGTATCGTCCCGCACAACTACAACCTCCACACTTGCCAGc > 2:437930/1‑150 (MQ=255)
ccccGGCGGCCACGCCATCGACCTGCCCAAAGGCGCCCCGCCATTGGACTTCGCCCACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCCCGACCAGCAACGGCCGTCTCGTGCCCCCGCACCACAGCCTGCACCCTGTCGAGcg > 2:381158/1‑149 (MQ=255)
ccccGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCa > 2:221347/1‑150 (MQ=255)
ccGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCa < 1:13815/148‑1 (MQ=255)
ccGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCa > 2:13815/1‑148 (MQ=255)
ccGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCCACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAgg > 2:580375/1‑150 (MQ=255)
cGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGt < 1:239214/150‑1 (MQ=255)
ccACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATc > 2:1146866/1‑106 (MQ=255)
ccACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATc < 1:1146866/106‑1 (MQ=255)
gCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCCACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGatcatc > 2:507769/1‑150 (MQ=255)
cATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCAc > 2:415159/1‑150 (MQ=255)
aCCTGCCCAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCagca > 2:1155053/1‑150 (MQ=255)
cAAAGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATAGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGATATCATCACCAGCAAGCACgg < 1:712715/150‑1 (MQ=255)
aaaGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCGGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGc > 1:80467/1‑150 (MQ=255)
aaaGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTg > 2:876533/1‑90 (MQ=255)
aaaGGCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTg < 1:876533/90‑1 (MQ=255)
ggCGCCACGCCATTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAAc < 2:737135/150‑1 (MQ=255)
gccacgccaTTGGACTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAAcccc > 2:1062472/1‑150 (MQ=255)
aTTGGACTTCGCCCACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCAGCCGTGa > 1:960445/1‑150 (MQ=255)
ggACTTCGCCCACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCAGCCGTGACTg < 1:507769/150‑1 (MQ=255)
cTTCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCAGCCGTGACTGGtt < 1:527681/150‑1 (MQ=255)
ttCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAg < 2:1273196/99‑1 (MQ=255)
ttCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAg > 1:1273196/1‑99 (MQ=255)
ttCGTCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCAGCCGTGACTGGTTg > 1:662382/1‑150 (MQ=255)
cGCCCACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAACAGCTGATGACGTTCACCAGCAAGCACGGCAACCCCAGCCGTGACTGGTTGaa < 1:349838/150‑1 (MQ=255)
gtcTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCAGc < 2:349898/133‑1 (MQ=255)
gtcTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCAGc > 1:349898/3‑135 (MQ=255)
cTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCAGCCGTGACTGGTTGAACTc < 2:80467/150‑1 (MQ=255)
|
AGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCAGCCGTGACTGGTTGAACTC > NC_002947/1852122‑1852419
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
