Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F91 I0 R1 918 53.4 2573728 94.2% 2424451 136.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,162,148 T→A 11.5% intergenic (‑48/+29) PP_3663 ← / ← pssA GGDEF domain‑containing protein/CDP‑diacylglycerol‑‑serine O‑phosphatidyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,162,1480TA11.5% 148.1 / 11.3 52intergenic (‑48/+29)PP_3663/pssAGGDEF domain‑containing protein/CDP‑diacylglycerol‑‑serine O‑phosphatidyltransferase
Reads supporting (aligned to +/- strand):  ref base T (20/26);  new base A (3/3);  total (23/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.66e-01

CCGACTGTATGCGATCGTGATCACGTTCAGAGGGGCCTTGCACTGTTGCCTGCACATTGTAATCCAGCCCCTGACAAAGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGATCAACGGCTCCAACCGGCTATAGCGCAACCGACGCAGAAACTTGCGCACTTGCTTGGGATGTTCGGCCTTCTCCCCCAGCGCCTCTGCCGAACCGATTCTCGG  >  NC_002947/4162001‑4162296
                                                                                                                                                   |                                                                                                                                                    
ccGACTGTATGCGATCGTGATCACGTTCAGAGGGGCCTTGCACTGTTGCCTGCACATTGTAATCCAGCCCCTGACAAAGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGc                                                                                                                                                    >  1:54198/1‑150 (MQ=255)
   aCTGTATGCGATCGTGATCACGTTCAGAGGGGCCTTGCACTGTTGCCTGCACATTGTAATCCAGCCCCTGACAAAGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCgg                                                                                                                                                 <  2:924020/150‑1 (MQ=255)
      gTATGCGATCGTGATCACGTTCAGAGGGGCCTTGCACTGTTGCCTGCACATTGTAATCCAGCCCCTGACAAAGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGa                                                                                                                                              <  1:985719/150‑1 (MQ=255)
       tATGCGATCGTGATCACGTTCAGAGGGGCCTTGCACTGTTGCCTGCACATTGTAATCCAGCCCCTGACAAAGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGAc                                                                                                                                             >  1:1014069/1‑150 (MQ=255)
       tATGCGATCGTAATAAAGCTCAGAGGGGCCCTGCTATGTTGCCTACACATAGTAATCCAGCCCCTGACAAAAGGCCGTGGGCAGCAATATCATAATGCCCTCAATGACCCGTGCGCACCACTCTGCCCCTGCCCATGACTTGCCGGCGaa                                                                                                                                             >  2:903380/1‑149 (MQ=255)
         tGCGATCGTGATCACGTTCAGAGGGGCCTTGCACTGTTGCCTGCACATTGTAATCCAGCCCCTGACAAAGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACGCTGACCCTGTCGCCGGCAAGCCAGCGACAg                                                                                                                                           <  1:247315/150‑1 (MQ=255)
          gCGATCGTGATCACGTTCAGAGGGGCCTTGCACTGTTGCCTGCACATTGTAATCCAGCACCTGACAAAGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAgg                                                                                                                                          <  2:1003748/150‑1 (MQ=255)
                 tGATCACGTTCAGAGGGGCCTTGCACTGTTGCCTGCACATTGTAATCCAGCCCCTGACAAAGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACa                                                                                                                                            <  2:159609/141‑1 (MQ=255)
                 tGATCACGTTCAGAGGGGCCTTGCACTGTTGCCTGCACATTGTAATCCAGCCCCTGACAAAGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACa                                                                                                                                            >  1:159609/1‑141 (MQ=255)
                     cACGTTCAGAGGGGCCTTGCACTGTTGCCTGCACATTGTAATCCAGGCCCTGACAAAGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAgg                                                                                                                               <  1:277044/150‑1 (MQ=255)
                            agagGGGCCTTGCACTGTTGCCTGCACATTGTAATCCAGCCCCTGACAAAGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTc                                                                                                                        >  2:614079/1‑150 (MQ=255)
                               ggggCCTTGCACTGTTGCCTGCACATTGTAATCCAGCCCCTGACAAAGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCaga                                                                                                                     <  2:1067862/150‑1 (MQ=255)
                                         aCTGTTGCCTGCACATTGTAATCCAGCCCCTGACAAAGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCtt                                                                                                           <  1:1143351/150‑1 (MQ=255)
                                          cTGTTGCCTGCACATTGTAATCCAGCCCCTGACAAAGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTg                                                                                                          >  1:553040/1‑150 (MQ=255)
                                           tGTTGCCTGCACATTGTAATCCAGCCCCTGACAAAGGGCTGTAGGCAGCAATATCATAATGACATAAATGCGCGGTGCGCACCACTCTGGACCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGa                                                                                                         <  1:237202/150‑1 (MQ=255)
                                           tGTTGCCTGCACATTGTAATCCAGCCCCTGACAAAGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGa                                                                                                         <  2:952523/150‑1 (MQ=255)
                                            gTTGCCTGCACATTGTAATCCAGCCCCTGACAAAGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCaga                                                                                                                     <  2:1270062/137‑1 (MQ=255)
                                            gTTGCCTGCACATTGTAATCCAGCCCCTGACAAAGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCaga                                                                                                                     >  1:1270062/1‑137 (MQ=255)
                                             ttGCCTGCACATTGTAATCCAGCCCCTGACAAAGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCAATGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGATc                                                                                                       <  1:1122231/150‑1 (MQ=255)
                                                 cTGCACATTGTAATCCAGCCCCTGACAAAGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGATTGCCGGAAAATGGGACAGTAAAGGCGACGTGAGAGCACCCGCTGGATCAACg                                                                                                   >  2:1105175/1‑150 (MQ=255)
                                                  tGCACATTGTAATCCAGCCCCTGACAAAGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCCGGCAAGCCAGCGACAGGGCCAga                                                                                                                                    >  2:917185/1‑115 (MQ=38)
                                                  tGCACATTGTAATCCAGCCCCTGACAAAGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCCGGCAAGCCAGCGACAGGGCCAga                                                                                                                                    <  1:917185/116‑2 (MQ=38)
                                                    cacaTTGTAATCCAGCCCCTGACAAAGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGATCAACGGCt                                                                                                <  1:1105175/150‑1 (MQ=255)
                                                      caTTGTAATCCAGCCCCTGACAAAGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGATCAACGGCTcc                                                                                              <  1:576220/150‑1 (MQ=255)
                                                            aaTCCAGCCCCTGACAAAGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGATCAACGGCTCCAACCgg                                                                                        <  2:702432/150‑1 (MQ=255)
                                                             aTCCAGCCCCTGACAAAGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGATCAACGGCTCCAACCGGc                                                                                       >  1:322647/1‑150 (MQ=255)
                                                              tCCAGCCCCTGACAAAGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGATCaa                                                                                                     >  1:402634/1‑135 (MQ=255)
                                                              tCCAGACCCTGACAAAGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGATCaa                                                                                                     <  2:402634/135‑1 (MQ=255)
                                                                 aGCCCCTGACAAAGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGATCAACGGCTCCACCCGGCtata                                                                                   <  2:1014069/150‑1 (MQ=255)
                                                                        gACAAAGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGATCAACGGCTCCAACCGGCTATAGCGCAAc                                                                            <  2:553040/150‑1 (MQ=255)
                                                                           aaaGGGCCGTGGGCAGCAATATCATAGTGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGATCAACGGCTCCAACCGGCTATAGCGCAACCGa                                                                         >  1:355681/1‑150 (MQ=255)
                                                                           aaaGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGTGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGATCAACGGCTCCAACCGGCTATAGCGCAACCGa                                                                         >  1:258808/1‑150 (MQ=255)
                                                                           aaaGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAg                                                                                                                                <  1:1051795/95‑1 (MQ=255)
                                                                           aaaGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAg                                                                                                                                >  2:1051795/1‑95 (MQ=255)
                                                                            aaGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGTGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGATCAACGGCTCCAACCGGCTATAGCGCAACCGAc                                                                        <  2:258808/150‑1 (MQ=255)
                                                                                         agcaATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCg                                                                                                                                                  >  1:560438/1‑63 (MQ=255)
                                                                                         agcaATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCg                                                                                                                                                  <  2:560438/63‑1 (MQ=255)
                                                                                                tCATATTGATATCAGTGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGCCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGATCAACGGCTCCAACCGGCTATAGCGCAACCGACGCAGAAACTTGCGCActtgc                                                    <  2:585329/150‑1 (MQ=255)
                                                                                                tCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGATCAACGGCTCCAACCGGCTATAGCGCAACCGACGCAGAAACTTGCGCActtgc                                                    >  2:867047/1‑150 (MQ=255)
                                                                                                          aTCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGATCAACGGCTCCAACCGGCTATAGCGCAACCGACGCAGAAACTTGCGCACTTGCTTGGGATGtt                                          >  2:1000878/1‑150 (MQ=255)
                                                                                                            cAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGATCAACGGCTCCAACCGGCTATAGCGCAACCGACGCAGAAACTTGCGCACTTGCTTGGGATGTTCg                                        <  1:614079/150‑1 (MQ=255)
                                                                                                                         gcACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGATCAACGGCTCCAACCGGCTATAGCGCAACCGACGCAGAAACTTGCGCACTTGCTTGGGATGTTCGGCCTTCTCCCCCa                           >  1:1239703/1‑150 (MQ=255)
                                                                                                                           accacTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGATCAACGGCTCCAACCGGCTATAGCGCAACCGACGCAGAAACTTGCGCACTTGCTTGGGATGTTCGGCCTTCTCCCCCTgc                         <  1:1285445/150‑1 (MQ=255)
                                                                                                                              acTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGATCAACGGCTCCAACCGGCTATAGCGCAACCGACGCAGAAACTTGCGCACTTGCTTGGGATGTTCGGCCTTCTCCCCCAGCGcc                      >  2:383065/1‑150 (MQ=255)
                                                                                                                                tctGGCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGATCAACGGCTCCAACCGGCTATAGCGCAACCGACGCAGAAACTTGCGCACTTGCTTGGGATGTTCGGCCTTCTCCCCCAGCGCctc                    >  1:1136405/1‑150 (MQ=255)
                                                                                                                                   gacccTGTCGCCGGCAAGCCAGCGACAGGTTCAGTACAGGCGATGTCAGAGCACCCGCTTGATCAACGGCTCCAACCGGCTATAGCGCAACCGAc                                                                        <  2:1218546/93‑1 (MQ=37)
                                                                                                                                   gacccTGTCGCCGGCAAGCCAGCGACAGGTTCAGTACAGGCGATGTCAGAGCACCCGCTTGATCAACGGCTCCAACCGGCTATAGCGCAACCGAc                                                                        >  1:1218546/3‑95 (MQ=37)
                                                                                                                                       cTGTCGCCGGCAAGCCAGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGATCAACGGCTCCAACCGGCTATAGCGCAACCGACGCAGAAACTTGCGCACTTGCTTGGGATGTTCGGCCTTCTCCCCCAGCGCATCTGCCGaa             >  1:54982/1‑150 (MQ=255)
                                                                                                                                        tGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGATCAACGGCTCCAACCGGCTATAGCGCAACCGACGCAGAAACTTGCGCACTTGCTTGGGATGTTCGGCCTTCTCCCCCAGCGCCTCTGCCGAAc            <  1:893109/150‑1 (MQ=255)
                                                                                                                                                gCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGATCAACGGCTCCAACCGGCTATAGCGCAACCGACGCAGAAACTTGCGCACTTGCTTGGGATGtt                                          <  1:1277944/112‑1 (MQ=255)
                                                                                                                                                gCTTGCCGGCGAAAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGATCAACGGCTCCAACCGGCTATAGCGCAACCGACGCAGAAACTTGCGCACTTGCTTGGGATGtt                                          >  2:1277944/1‑112 (MQ=255)
                                                                                                                                                 cTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGATCAACGGCTCCAACCGGCTATAGCGCAACCGACGCAGAAACTTGCGCACTTGCTTGGGATGTTCGGCCTTCTCCCCCAGCGCCTCTGCCGAACCGATTCTCg   <  1:867047/150‑1 (MQ=255)
                                                                                                                                                  ttGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGATCAACGGCTCCAACCGGCTATAGCGCAACCGACGCAGAAACTTGCGCACTTGCTTGGGATGTTCGGCCTTCTCCCCCAGCGCCTCTGCCGAAc            <  2:923300/140‑1 (MQ=255)
                                                                                                                                                  ttGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGATCAACGGCTCCAACCGGCTATAGCGCAACCGACGCAGAAACTTGCGCACTTGCTTGGGATGTTCGGCCTTCTCCCCCAGCGCCTCTGCCGAAc            >  1:923300/1‑140 (MQ=255)
                                                                                                                                                  ttGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGATCAACGGCTCCAACCGGCTATAGCGCAACCGACGCAGAAACTTGCGCACTTGCTTGGGATGTTCGGCCTTCTCCCCCAGCGCCTCTGCCGAACCGATTCTCgg  <  2:322647/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CCGACTGTATGCGATCGTGATCACGTTCAGAGGGGCCTTGCACTGTTGCCTGCACATTGTAATCCAGCCCCTGACAAAGGGCCGTGGGCAGCAATATCATAATGACATCAATGCGCGGTGCGCACCACTCTGGCCCTGTCGCTGGCTTGCCGGCGACAGGGTCAGTACAGGCGATGTCAGAGCACCCGCTTGATCAACGGCTCCAACCGGCTATAGCGCAACCGACGCAGAAACTTGCGCACTTGCTTGGGATGTTCGGCCTTCTCCCCCAGCGCCTCTGCCGAACCGATTCTCGG  >  NC_002947/4162001‑4162296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A