Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F56 I1 R1	5	46.6	2293046	94.7%	2171514	133.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,647,612	G→A	D384D (GAC→GAT)	oprB‑II ←	carbohydrate‑selective porin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,647,612	0	G	A	100.0%	85.0 / NA	28	D384D (GAC→GAT)	oprB‑II	carbohydrate‑selective porin
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (14/14); total (14/14)

GGCGCCATCGACCTGCGACACCCCGCCCGGGTGGCGGATGTACTGCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAGTCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATCGAAAGGCCCTTTGAATACCAGTCCTGC  >  NC_002947/1647465‑1647746

ggCGCCATCGACCTGCGACACCCCGCCCGGGTGGCGGATGTACTGCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATc                                                                                                                                      >  1:1105838/1‑150 (MQ=255)

         gACCTGCGACACCCCGCCCGGGTGGCGGATGTACTGCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCGTGCACTGGCATGAAGCGCGGGTTGTCGTAATCATAGAggac                                                                                                                             >  2:187784/1‑148 (MQ=255)

                     cccgcccgGGTGGCGGATGTACTGCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCAGAATCATATAGGCCGGCGGCCTGGtt                                                                                                                 >  2:1123182/1‑150 (MQ=255)

                               tGGCGGATGTACTGCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAggcgg                                                                                                       <  1:1012732/150‑1 (MQ=255)

                                            gCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGAt                                                                                          >  1:189307/1‑150 (MQ=255)

                                                gTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGtt                                                                                                                 <  2:656670/123‑1 (MQ=255)

                                                gTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGtt                                                                                                                 >  1:656670/1‑123 (MQ=255)

                                                  tGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAg                                                                                    <  1:1123182/150‑1 (MQ=255)

                                                   ggggCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAgg                                                                                   <  1:187784/150‑1 (MQ=255)

                                                     ggCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGt                                                                                 <  2:596019/150‑1 (MQ=255)

                                                         tACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGAc                                                                             <  1:713255/150‑1 (MQ=255)

                                                                          ccAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGc                                                                                             <  1:1134701/117‑1 (MQ=255)

                                                                          ccAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGc                                                                                             >  2:1134701/1‑117 (MQ=255)

                                                                           cAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGc                                                           >  1:126100/1‑150 (MQ=255)

                                                                               tGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGaaa                                                       >  1:589366/1‑150 (MQ=255)

                                                                                 aaTGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAAcc                                                     <  2:189307/150‑1 (MQ=255)

                                                                                    gCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGAt                                                  <  1:30804/150‑1 (MQ=255)

                                                                                      cGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATgt                                                >  1:469986/1‑150 (MQ=255)

                                                                                         aaTACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCACGACGCACTGCTCGTGGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGTCCAGTGCGAAACCGATgtcgt                                             >  2:160949/1‑150 (MQ=255)

                                                                                          aTACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATgtcgtc                                            <  2:1105838/150‑1 (MQ=255)

                                                                                                           aCTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATCGa                           >  2:227143/1‑150 (MQ=255)

                                                                                                                   tCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATCGAAAGGCCCt                   >  2:917685/1‑150 (MQ=255)

                                                                                                                    ccTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATCGAAAGGCCCtt                  <  2:843036/150‑1 (MQ=255)

                                                                                                                     cTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATCGAAAGGCCCttt                 <  1:227143/150‑1 (MQ=255)

                                                                                                                      tGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATCGAAAGGCCCTTTg                <  2:469986/150‑1 (MQ=255)

                                                                                                                          cTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATCGAAAGGCCCTTTGAATa            <  1:346677/150‑1 (MQ=255)

                                                                                                                                    gCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATCGAAAGGCCCTTTGAATACCAGTcctgc  >  2:663525/1‑150 (MQ=255)

                                                                                                                                                gTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCgg                                                                   >  1:680761/1‑73 (MQ=255)

GGCGCCATCGACCTGCGACACCCCGCCCGGGTGGCGGATGTACTGCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAGTCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATCGAAAGGCCCTTTGAATACCAGTCCTGC  >  NC_002947/1647465‑1647746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A