Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F91 I5 R1 6 62.9 3170796 93.7% 2971035 131.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 4,964,714 C→G R230P (CGT→CCT)  fleQ ← transcriptional regulator FleQ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,964,7140CG98.6% 241.4 / NA 70R230P (CGT→CCT) fleQtranscriptional regulator FleQ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (39/30);  minor base T (0/1);  total (39/31)
Fisher's exact test for biased strand distribution p-value = 4.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCACGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCGGGATCGCGCCACAGTTGACCGGCACGAACGGCGCTTCGCGGCGCTTGGAATGGTAGTGCAGGTTGCGCGCTACC  >  NC_002947/4964570‑4964857
                                                                                                                                                |                                                                                                                                                
aTGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCACCGATTTCGTCGAGGAAAAGGGTACCGCCGTTGGCCGGTTCGAAACGCCGGGCAGGGcgg                                                                                                                                             >  1:850756/1‑148 (MQ=255)
 tGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCAGGGCTgg                                                                                                                                            <  2:551629/150‑1 (MQ=255)
 tGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCAGGGCTgg                                                                                                                                            >  1:260784/1‑150 (MQ=255)
 tGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCAGGGCTgg                                                                                                                                            >  1:551629/1‑150 (MQ=255)
   cTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGGACCTGCTGATGCTGGGGCAGCTTGACCTGTATGGGTAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCAGGGCTGGTg                                                                                                                                          >  1:30023/1‑150 (MQ=25)
          tCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCAGGGCTGGTGATGGccc                                                                                                                                   <  1:150790/150‑1 (MQ=255)
            ttgttgCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGAGTAGCGCCGTTGGCCAGTTCGAAACGCCCGGCAGGGCTGGTGATGGCCCCg                                                                                                                                 >  1:1532619/1‑150 (MQ=255)
              gttgCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCAGGGCGGGTGATGGCCCCGGt                                                                                                                               >  1:489282/1‑150 (MQ=255)
                      ccACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGGATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCAGGGCTGGTGATGGCCCCGGT‑AAAGgcgc                                                                                                                      <  1:440427/150‑1 (MQ=255)
                      ccACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCATGGCTGGTGATGGCCCCGGT‑AAAGgcgc                                                                                                                      <  1:1110708/150‑1 (MQ=255)
                              tCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCg                                                                                                              >  2:227018/1‑150 (MQ=255)
                                      aCGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCGTGGCCGaa                                                                                                      <  2:439179/150‑1 (MQ=255)
                                        gCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCGTGGCCGAACa                                                                                                    >  2:229637/1‑150 (MQ=255)
                                        gCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCGTGGCCGAACa                                                                                                    >  1:584839/1‑150 (MQ=255)
                                           ccTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCGt                                                                                                             <  2:45160/138‑1 (MQ=255)
                                           ccTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCGt                                                                                                             >  1:45160/1‑138 (MQ=255)
                                             tGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCGTGGCCGAACAGTTCg                                                                                               >  2:1412234/1‑150 (MQ=255)
                                                accacaCGCAGCAGCGTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCCCCGTTGGCTAGTTCGAAACGCCCGTCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCtt                                                                                            <  1:1288432/148‑1 (MQ=39)
                                                  aacgctcagcagCTTTTCCTTTAACGGCAAGGGGATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCAGGGCTGGTGATGGCCCTGGT‑AAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTc                                                                                          <  2:260784/144‑1 (MQ=255)
                                                   acacGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTtc                                                                                                                 <  2:1074385/126‑1 (MQ=255)
                                                   acacGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTtc                                                                                                                 >  1:1074385/1‑126 (MQ=255)
                                                     acGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCcag                                                                                       >  1:201245/1‑150 (MQ=255)
                                                     acGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCcag                                                                                       >  2:1253553/1‑150 (MQ=255)
                                                     acGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCcag                                                                                       <  2:1039112/150‑1 (MQ=255)
                                                      cGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCcagc                                                                                      <  1:1412234/150‑1 (MQ=255)
                                                         agcagcTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCTGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTcc                                                                                         <  2:974200/144‑1 (MQ=255)
                                                         agcagcTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTcc                                                                                         >  1:974200/1‑144 (MQ=255)
                                                         agcagcTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCagcagc                                                                                   <  1:229637/150‑1 (MQ=255)
                                                          gcagcTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCGTGGCCGAACa                                                                                                    >  2:729740/1‑132 (MQ=255)
                                                          gcagcTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCGTGGCCGAACa                                                                                                    <  1:729740/132‑1 (MQ=255)
                                                             gcTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCgg                                                                               >  1:138689/1‑150 (MQ=255)
                                                                    cTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCGGGAt                                                                        >  1:1498108/1‑150 (MQ=255)
                                                                       cATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCGGGATcgc                                                                     <  1:227018/150‑1 (MQ=255)
                                                                         tCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACCCCCGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCGGGCTCgcgc                                                                   >  1:163003/1‑150 (MQ=255)
                                                                          cGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTtctc                                                                                                               >  1:289453/1‑105 (MQ=255)
                                                                          cGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTtctc                                                                                                               <  2:289453/105‑1 (MQ=255)
                                                                                             aTTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCGGGATCGCGCCACAGTTGACCGGCACTAAc                                               <  2:1498108/150‑1 (MQ=255)
                                                                                                  gtcgAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCGGGATCGCGCCACAGTTGACCGGCACGAACGgcgc                                          <  2:584839/150‑1 (MQ=255)
                                                                                                  gtcgAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCGGGATCGCGCCACAGTTGACCGGCACGAACGgcgc                                          >  2:1205330/1‑150 (MQ=255)
                                                                                                     gAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCGGGATCGCGCCACAGTTGACCGGCACGAACGGCGCTTc                                       >  2:1548840/1‑150 (MQ=255)
                                                                                                         aaCAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCcagca                                                                                     <  1:1480526/100‑1 (MQ=255)
                                                                                                         aaCAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCcagca                                                                                     >  2:1480526/1‑100 (MQ=255)
                                                                                                            aGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCGGGATCGCGCCACAGTTGACCGGCACGAACGGCGCTTCGCGgagc                                >  2:1467016/1‑150 (MQ=255)
                                                                                                              ggTACCGCCGTTGGCCAGTTCGAAACGCCCGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCGGGATCGCGCCACAGTTGACCGGCACGaa                                                >  1:882770/1‑132 (MQ=255)
                                                                                                              ggTACCGCCGTTGGCCAGTTCGAAACGCCCGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCGGGATCGCGCCACAGTTGACCGGCACGaa                                                <  2:882770/132‑1 (MQ=255)
                                                                                                              ggTACCGCCGTTGGCCAGTTCGAAACGCCCGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCGGGATCGCGCCACAGTTGACCGGCACGAACGGCGCTTCGCGGCGCtt                              <  1:1205330/150‑1 (MQ=255)
                                                                                                                   cgccgTTGGCCAGTTCGAAACGCCCGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCGGGATCGCGCCACAGTTGACCGGCACGAACGGCGCTTCGCGGCGCTTGGAAt                         <  1:1548840/150‑1 (MQ=255)
                                                                                                                   cgccgTTGGCCAGTTCGAAACGCCCGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCGTGGCCGAAAAGTTCGCTTTCCAGCAGCTCGGCCGGGATCGCGCCACAGTTGACCGGCACGAACGGCGCTTCGCGGCGCTTGGAAt                         >  1:1002439/1‑150 (MQ=255)
                                                                                                                     ccgTTGGCCCGTTCGAAACGCCCGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCGGGATCGCGCCACAGTTGACCGGCACGAACGGCGCTTCGCGGCGCTTGGAATgg                       <  2:281639/150‑1 (MQ=255)
                                                                                                                        ttGGCCAGTTCGAAACGCCCGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCGGGATCGCGCCACAGTTGACCGGCACGAACGGCGCTTCGCGGCGCTTGGAATGGTAg                    >  1:1298295/1‑150 (MQ=255)
                                                                                                                        ttGGCCAGTTCGAAACGCCCGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCGGGATCGCGCCACAGTTGACCGGCACGAACGGCGCTTCGCGGCGCTTGGAATGGTAg                    <  2:489282/150‑1 (MQ=255)
                                                                                                                            ccAGTTCGAAACGCCCGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCGGGATCGCGCCACAGTTGACCGGCACGAACGGCGCTTCGCGGCGCTTGGAATGGTAGTGCa                <  1:1253553/150‑1 (MQ=255)
                                                                                                                             cAGTTCGAAACGCCCGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCGGGATCGCGCCACAGTTGACCGGCACGAACGGCGCTTCGCGGCGCTTGGAATGGTAGTGCAg               >  1:588784/1‑150 (MQ=255)
                                                                                                                              aGTTCGAAACGCCCGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCGGGATCGCGCCACAGTTGACCGGCACGAACGGCGCTTCGCGGCGCTTGGAATGGTAGTGCAgg              >  1:527430/1‑150 (MQ=255)
                                                                                                                              aGTTCGAAACGCCCGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCGGGATCGCGCCACAGTTGACCGGCACGAACGGCGCTTCGCGGCGCTTGGAATGGTAGTGCAgg              >  2:784070/1‑150 (MQ=255)
                                                                                                                               gTTCGAAACGCCCGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAAATCGGCCGGGATCGCGCCACAGTTGACCGGCACGAACGGCGCTTCGCGGCGCTTGTAATGTGAGTGCAGGt             >  1:1139914/1‑150 (MQ=255)
                                                                                                                                ttCGAAACGCCCGGCAGGGCTGGTGATGGCCCCGGTAAAAGGCGCCCTTCTCGTGGc                                                                                                          >  2:1118197/1‑57 (MQ=38)
                                                                                                                                ttCGAAACGCCCGGCAGGGCTGGTGATGGCCCCGGTAAAAGGCGCCCTTCTCGTGGc                                                                                                          <  1:1118197/57‑1 (MQ=38)
                                                                                                                                   gAAACGCCCGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCGGGATCGCGCCACAGTTGACCGGCACGAACGGCGCTTCGCGGCGCTTGGAATGGTAGTGCAGGTTgcg         >  2:1274968/1‑150 (MQ=255)
                                                                                                                                    aaaCGCCCGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCGGGATCGCGCCACAGTTGACCGGCACGAACGGCGCTTCGCGGCGCTTGGAATGGTAGTGCAGGTTgcgg        >  2:1395163/1‑149 (MQ=255)
                                                                                                                                    aaaCGCCCGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCGGGATCGCGCCACAGTTGACCGGCACGAACGGCGCTTCGCGGCGCTTGGAATGGTAGTGCAGGTTgcgc        <  2:527430/150‑1 (MQ=255)
                                                                                                                                    aaaCGCCCGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCGGGATCGCGCCACAGTTGACCGGCACGAACGGCGCTTCGCGGCGCTTGAAATGGTAGTGCAGGTTgcgc        >  2:1394403/1‑150 (MQ=255)
                                                                                                                                    aaaCGCCCGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCGGGATCCCGCCACACAGTACCTTCACGAACGGCGCTTCGCGGCGCTTGGAATGGTAGTGCAGGTTgcgc        <  2:1329592/150‑1 (MQ=255)
                                                                                                                                    aaaCGCCCGGCAGGGCTGGTGATGCCACCGGTCACAGGC‑TCGGCCTCGTAAGTGGACAGTTCGCTTTCCCGCAGCGCGGCCGGGATCGCGCCACAGTTGACCGGCACGAACGGCGCTTCGCGGCGCTTGGAATGGTAGTGCAGGTTgcgc        <  1:355104/150‑1 (MQ=255)
                                                                                                                                     aaCGCCCGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCGGGATcgc                                                                     >  2:49257/1‑88 (MQ=255)
                                                                                                                                     aaCGCCCGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCGGGATcgc                                                                     <  1:49257/88‑1 (MQ=255)
                                                                                                                                          ccGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCGGGATCGCGCCACAGTTGACCGGCACGAACGGCGCTTCGCGGCGCTTGGAATGGTAGTGCAGGTTGCGCGCTa    >  1:1190976/1‑148 (MQ=255)
                                                                                                                                          ccGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCGGGATCGCGCCACAGTTGACCGGCACGAACGGCGCTTCGCGGCGCTTGGAATGGTAGTGCAGGTTGCGCGCTa    <  2:1190976/148‑1 (MQ=255)
                                                                                                                                          ccGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCGGGATCGCGCCACAGTTGACCGGCACGAACGGCGCTTCGCGGCGCTTGGAATGGTAGTGCAGGTTGCGCGCTAcc  >  1:788111/1‑150 (MQ=255)
                                                                                                                                          ccGGCAGGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCACGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCGGGATCGCGCCACAGTTGACCGGCACGAACGGCGCTTCGCGGCGCTTGGAATGGTAGTGCAGGTTGCGCGCTAcc  >  2:690104/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                
ATGCTTTGGGTCTTGTTGCTACCCACGCGCTCGAAGGTACGCTCCTGCAGCACACGCAGCAGCTTGACCTGCATCGGCAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCACGGCTGGTGATGGCCCCGGT‑AAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCGGGATCGCGCCACAGTTGACCGGCACGAACGGCGCTTCGCGGCGCTTGGAATGGTAGTGCAGGTTGCGCGCTACC  >  NC_002947/4964570‑4964857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A