Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F71 I1 R1
|
31 |
24.1 |
564604 |
94.3% |
532421 |
140.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
USA300TCH1516_ALE |
1,996,168 |
A→G |
noncoding (47/75 nt) |
USA300TCH1516_01859 ← |
tRNA‑Gly |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | USA300TCH1516_ALE | 1,996,168 | 0 | A | G | 100.0%
| 49.7
/ NA
| 19 | noncoding (47/75 nt) | USA300TCH1516_01859 | tRNA‑Gly |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (6/13); total (6/13) |
CGCAAATATTAAATTATGGAGCGGAAGATAGGATTTACACCTATACCTCATTCCAGGAAGGAATGTATTCTAAGAGTTGAAATACTCCCGCATTATTATTAAATTATGGAGCGGAAGATAGGATTTGCACCTATACCTCGTTCCGGGAAGGAACGTGTTCTAAAAGTTGAACTACTCCCGCATAAACCTGGAGGCGGCAACCGGATTTGAACCGGTGATAAAGGTTTTGCAGACCTCTGCCTTACCACTTGGCTATGCCGCC > USA300TCH1516_ALE/1996034‑1996295
|
cGCAAATATTAAATTATGGAGCGGAAGATAGGATTTACACCTATACCTCATTCCAGGAAGGAATGTATTCTAAGAGTTGAAATACTCCCGCATTATTATTAAATTATGGAGCGGAAGATAGGATTTGCACCTATGCCTCGt > 1:164787/1‑141 (MQ=255)
cGCAAATATTAAATTATGGAGCGGAAGATAGGATTTACACCTATACCTCATTCCAGGAAGGAATGTATTCTAAGAGTTGAAATACTCCCGCATTATTATTAAATTATGGAGCGGAAGATAGGATTTGCACCTATGCCTCGt > 1:187968/1‑141 (MQ=255)
cGCAAATATTAAATTATGGAGCGGAAGATAGGATTTACACCTATACCTCATTCCAGGAACGAATGTATTCTAAGAGTTGAAATACTCCCGCATTATTATTAAATTATGGAGCGGAAGATAGGATTTGCACCTATGCCTCGt < 1:78095/141‑1 (MQ=255)
gCAAATATTAAATTATGGAGCGGAAGATAGGATTTACACCTATACCTCATTCCAGGAAGGAATGTATTCTAAGAGTTGAAATACTCCCGCATTATTATTAAATTATGGAGCGGAAGATAGGATTTGCACCTATGCCTCGtt > 1:97552/1‑141 (MQ=255)
aTGGAGCGTAAGATAGGAGTTACACCTATACCTCATTCCAGGAAGGAAAGTATTTTAAGGGTTGAAATACTCCCGCATTATTAGTAAATTATGGAGCGGAAGATAGGATTTGCACCTATGCCTCGTTCCGGGAAGGAACgt < 2:85065/141‑1 (MQ=18)
tACACCTATACCTCATTCCAGGAAGGAATGTATTCTAAGAGTTGAAATACTCCCGCATTATTCTTCAATTATGAACACGGCGAGAGAATTTGCACCTATGCCTCGTTCCGGCGACGAACGTGTTCTCAAAGTTGAaccact > 2:273829/1‑141 (MQ=255)
cacCTATACCCCATTGCAGAAAGGCATGTATTATAAGAGGTGAAATACACCCGCATTATTATTAAATTATGGAGCGGAAGATAGGATTTGCACCTATGCCTCGTTCCGGGAAGGAACGTGTTCTAAAAGTTGAACTACTcc < 2:79790/141‑1 (MQ=21)
ccTATACCTCATTCCAGGAAGGAATGTATTCTAAGAGTTGAAATACTCCCGCATTATTATTAAATTATGGAGCGGAAGATAGGATTTGCACCTATGCCTCGTTCCGGGAAGGAACGTGTTCTAAAAGTTGAACTACTCCCg < 1:170837/141‑1 (MQ=255)
aggaaggaATGTATTCTAAGAGTTGAAATACTCCCGCATTATTCTTAAATTATGGAGCGGACGATAGGATTTGCACCTATGCCTCGTTCCGGGAAGGAACGTGTTCTAAACGATCAACTACTGCCGCATCAACCTGCAgac > 2:193645/1‑139 (MQ=21)
aaaTGTATTCTAAGAGTTGAAATACTCCCGCATTATTATTAAATTATGGAGCGGAAGATAGGATTTGCACCTATGCCTCGTTCCGGGAAGGAACGTGTTCTAAAAGTTGAACTACTCCCGCATAAACCTGGAGGCGGCAAc < 1:140514/140‑1 (MQ=255)
gAAATACTCCCGCATTATTATTAAATTATGGAGCGGAAGATAGGATTTGCACCTATGCCTCGTTCCGGGAAGGAACGTGTTCTAAAAGTTGAACTACTCCCGCATAAACCTGGAGGCGGCAACCGGATTTGAACCGGTGAt < 1:156958/141‑1 (MQ=255)
aaaTACTCCCGCATTATGATTAAATTATGGAGCGGAAGATAGGATTTGCACCTATGCCTCGTTCCGGGAAGGAACGTGTTCTAAAAGTTGAACTACTCCCGCATAAACCTGGAGGCGGCAACCGGATTTGAACCGGTGATa < 1:86097/141‑1 (MQ=255)
ccGCATTATTATTAAATTATGGAGCGGAAGATAGGATTTGCACCTATGCCTCGTTCCGGGAAGGAACGTGTTCTAAAAGTTGAACTACTCCCGCATAAACCTGGAGGCGGCAACCGGATTTGAACCGGTGATAAAGGtttt < 2:279204/141‑1 (MQ=255)
ttaAATTATGGAGCGGAAGATAGGATTTGCACCTATGCCTCGTTCCGGGAAGGAACGTGTTCTAAAAGTTGAACTACTCCCGCATAAACCTGGAGGCGGCAACCGGATTTGAACCGGTGATAAAGGTTTTGCAGACCTCTg < 2:8985/141‑1 (MQ=255)
ggAGGGGAAGATAGGATTTGCACCTATGCCTCGTTCCGGGAAGGAACGTGTTCTAAAAGTTGAACTACTCCCGCATAAACCTGGAGGCGGCAACCGGATTTGAACCGGTGATAAAGGTTTTGCAGACCTCTGCCTTACCAc < 2:97552/141‑1 (MQ=255)
gATAGGATTTGCACCTATGCCTCGTTCCGCGAAGGAACGTGTTCTAAAAGTTGAACTACCCCCGCCTAAACCTGTAGCCCGCAACCGGATTTCAACCCCTCATAAACCTTTTACATACCTCTGCCCTCCCCCttccctaca > 2:33759/1‑134 (MQ=12)
tAGGATTTGCACCTATGCCTCGTTCCGGGAAGGAACGTGTTCTAAAAGTTGAACTACTCCCGCATAAACCTGGAGGCGGCAACCGGATTTGAACCGGTGATAAAGGTTTTGCAGACCTCTGCCTTACCACTTGGCTATgcc < 1:215311/141‑1 (MQ=255)
gATTTGCACCTATGCCTCGTTCCGGGAAGTAACGTGTTCTAAAAGTTGAACTACTCCCGCATAAACCTGGAGGCGGCAACCGGATTTGAACCGGTGATAAAGGTTTTGCGGACCTCTGCCTTACCACTTGGCTATgccgcc < 2:12478/141‑1 (MQ=255)
gATTTGCACCTATGCCTCGTTCCGGGAAGGAACGTGTTCTAAAAGTTGAACTACTCCCGCATAAACCTGGAGGCGGCAACCGGATTTGAACCGGTGATAAAGGTTTTGCAGACCTCTGCCTTACCACTTGGCTATgccgcc < 1:273829/141‑1 (MQ=255)
|
CGCAAATATTAAATTATGGAGCGGAAGATAGGATTTACACCTATACCTCATTCCAGGAAGGAATGTATTCTAAGAGTTGAAATACTCCCGCATTATTATTAAATTATGGAGCGGAAGATAGGATTTGCACCTATACCTCGTTCCGGGAAGGAACGTGTTCTAAAAGTTGAACTACTCCCGCATAAACCTGGAGGCGGCAACCGGATTTGAACCGGTGATAAAGGTTTTGCAGACCTCTGCCTTACCACTTGGCTATGCCGCC > USA300TCH1516_ALE/1996034‑1996295
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
