Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F71 I1 R1
|
31 |
24.1 |
564604 |
94.3% |
532421 |
140.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
USA300TCH1516_ALE |
2,247,097 |
(T)6→7 |
intergenic (‑118/‑306) |
bltD_2 ← / → coaW |
Spermine/spermidine acetyltransferase/Type II pantothenate kinase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | USA300TCH1516_ALE | 2,247,091 | 1 | . | T | 100.0%
| 37.0
/ NA
| 13 | intergenic (‑112/‑312) | bltD_2/coaW | Spermine/spermidine acetyltransferase/Type II pantothenate kinase |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base T (6/7); total (6/7) |
CCCCTATCTTTTTATATATGAAGCATGAATCCCCATATTATTTTACCTATTATTTCACTACGATACCATTATTTGCTCTAAGTTAAATAAATTACTTATTTATA‑TTTTTTAAATTTCAAAATTAATGTACTATTCCTCATTACTTATCATTTTAATCAGAGACTTACAAATACACTGCGTACGAACTACTTTTAATATTGCGTCACTAACTAACA > USA300TCH1516_ALE/2246988‑2247202
|
ccccTATCTTTTTATATATGCAGCATGAATAACCACATTAATGGACCTATTATTTCACTACGATACCATTATTTGCTCTAAGTTAAATAAATTACTTATTTATATTTTTTTAAATTTCAAAATTAATGTACTATTCCTCAt > 1:122464/1‑141 (MQ=255)
cccTATCTTTTTATATATGAAGCATGAATCCCCATATTATTTTACCTATTATTTCACTACGATACCATTATTTGCTCTAAGTTAAATAAATTACTTATTTATATTTTTTTAATTTTCAAAATTAATTTACTATTCTTCttt > 2:216580/1‑141 (MQ=255)
cTATCTTTTTATATATGAAGCATGAATCCCCATATTATTTTACCTATTATTTCACTACGATACCATTATTTGCTCTAAGTTAAATAAATTACTTATTTATATTTTTTTAAATTTCACAATTAATGTACTATTCCTCtttcc > 2:28205/1‑139 (MQ=255)
atatatGAAGCATGAATCCCCATATTATTTTACCTATTATTTCACTACGATACCATTATTTGCTCTAAGTTAAATAAATTACTTATTTATATTTTTTTAAATTTCAAAATTAATGTACTATTCCTAATTACTTATCAtttt > 1:238532/1‑141 (MQ=255)
gaTCCCCATATTATTTTACCTATTATTTCACTACGATACCATTATTTGCTCTAAGTTAAATAAATTACTTATTTATATTTTTTTAAATTTCAAAATTAATGGACTATTCCTCATTACTTATCATTTTAATCAGAGACTTAc < 2:96991/140‑1 (MQ=255)
aaTCCCCATATTATTTTACCTATTATTTCACTACGATACCATTATTTGCTCTAAGTTAAATAAATTACTTATTTATATTTTTTTAAATTTCAAAATTAATGTACTATTCCTCATTACTTATCATTTTAATCAGAGACTTAc < 2:52480/141‑1 (MQ=255)
tttACCTATTATTTCACTACGATACCATTATTTGCTCTAAGTTAAATAAATTACTTATTTATATTTTTTTAAATTTCAAAATTAATGTACTATTCCTCATTACTTATCATTTTAATCAGAGACTTACAAATACACTGCGTa < 1:211396/141‑1 (MQ=255)
ttCACTACGATACCATTATTTGCTCTAAGTTAAATAAATTACTTATTTATATTTTTTTAAATTTCAAAATTAATGTACTATTCCTCATTACTTATCATTTTAATCAGAGACTTACAAATACACTGCGTACGAACTACtttt > 1:135642/1‑141 (MQ=255)
aCTACGATACCATTATTTGCTCTAAGTTAAATAAATTACTTATTTATATTTTTTTAAATTTCAAAATTAATGTACTATTCCTCATTACTTATCATTTTAATCAGAGACTTACAAATACACTGCGTACGAACTACTTTTAat < 1:90480/141‑1 (MQ=255)
aCCATTATTTGCTCTAAGTTAAATAAATTACTTATTTATATTTTTTTAAATTTCAAAATTAATGTACTATTCCTCATTACTTATCATTTTAATCAGAGACTTACAAATACACTGCGTACGAACTACTTTTAATATTGCGTc < 2:252577/141‑1 (MQ=255)
ccATTATTTGCTCTAAGTTAAATAAATTACTTATTTATATTTTTTTAAATTTCAAAATTAATGTACTATTCCTCATTACTTATCATTTTAATCAGAGACTTACAAATACACTGCGTACGAACTACTTTTAATATTGCGTCa < 2:122464/141‑1 (MQ=255)
tattTGCTCTAAGTTAAATAAATTACTTATTTATATTTTTTTAAATTTCAAAATTAATGTACTATTCCTCATTACTTATCATTTTAATCAGAGACTTACAAATACACTGCGTACGAACTACTTTTAATATTGCGTCactaa < 1:36981/141‑1 (MQ=255)
ctctAAGTTAAATAAATTACTTATTTATATTTTTTTAAATTTCAAAATTAATGTACTATTCCTCATTACTTATCATTTTAATCAGAGACTTACAAATACACTGCGTACGAACTACTTTTAATATTGCGTCACTAACTAACa > 1:140943/1‑141 (MQ=255)
|
CCCCTATCTTTTTATATATGAAGCATGAATCCCCATATTATTTTACCTATTATTTCACTACGATACCATTATTTGCTCTAAGTTAAATAAATTACTTATTTATA‑TTTTTTAAATTTCAAAATTAATGTACTATTCCTCATTACTTATCATTTTAATCAGAGACTTACAAATACACTGCGTACGAACTACTTTTAATATTGCGTCACTAACTAACA > USA300TCH1516_ALE/2246988‑2247202
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
