Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F44 I1 R1	12	24.7	574166	94.6%	543161	140.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	2,372,751	A→T	L342* (TTA→TAA)	pbuG ←	Guanine/hypoxanthine permease PbuG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,372,751	0	A	T	100.0%	54.4 / NA	19	L342* (TTA→TAA)	pbuG	Guanine/hypoxanthine permease PbuG
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (5/14); total (5/14)

AGGCTGGCACTGCAACTTCAAAACTTTTCCAGTTTATCTCAGCAAAGTTTGCAGCCATTAATACACCTACCACCACTAAGGCTGGTGTCGTAACTGCACTTGTTACAACAGCGATTAACGGACTAAAGAACAGCGCTAATAGGAAACAACAACCTGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTG  >  USA300TCH1516_ALE/2372614‑2372886

aGGCTGGCACTGCAACTTCAAAACTTTTCCAGTTTATCTCAGCAAAGTTTGCAGCCATTAATACACCTACCACCACTAAGGCTGGTGTCGTAACTGCACTTGTTACAACAGCGATTAACGGACTAAAGAACAGCGCTtata                                                                                                                                      >  1:221084/1‑141 (MQ=255)

    tGGCACTGCAACTTCAAAACTTTTCCAGTTTATCTCAGCAAAGTTTGCAGCCATTAATACCCCTACCACCACTAAGGCTGGTGTCGAATCTTTACTTGTTACAACAGCGATTAACGGACTAAAGAACAGCGCTTATAGGaa                                                                                                                                  >  1:122986/1‑141 (MQ=255)

      gCACTGCAACTTCAAAACTTTTCCAGTTTATCTCAGCAAAGTTTGCAGCCATTAATACACCTACCACCACTAAGGCTGGTGTCGTAACTGCACTTGTTACAACAGCGATTAACGGACTAAAGAACAGCGCTTATAGGAaac                                                                                                                                <  1:281197/141‑1 (MQ=255)

        aCTGCAACTTCAAAACTTTTCCAGTTTATCTCAGCAAAGTTTGCAGCCATTAATACACCTACCACCACTAAGGCTGGTGTCGTAACTGCACTTGTTACAACAGCGATTAACGGACTAAAGAACAGCGCTTATAGGAaacaa                                                                                                                              <  2:264374/141‑1 (MQ=255)

                          ttCCAGTTTATCTCAGCAAGGTTTGCAGCCATTAATACACCTACCACCACTAAGGCTGGTGTCGTAACTGCACTTGTTACAACAGCGATTAACGGACTAAAGAACAGCGCTTATAGGAAACAACAACCTGTTACGATACTa                                                                                                            <  2:195068/141‑1 (MQ=255)

                          ttCCAGTTTATCTCAGCAAAGTTTGCAGCCATTAATACACCTACCACCACTAAGGCTGGTGTCGTAACTGCACTTGTTACAACAGCGATTAACGGACTAAAGAACAGCGCTTATAGGAAACAACAACCTGTTACGATACTa                                                                                                            <  2:54450/141‑1 (MQ=255)

                              aGTTTATCTCAGCAAAGTTTGCAGCCATTAATACACCTACCACCACTAAGGCTGGTGTCGTAACTGCACTTGTTACAACAGCGATTAACGGACTAAAGAACAGCGCTTAGAGGAAACAACAACCTGTTACGATACTAGCaa                                                                                                        <  1:80883/141‑1 (MQ=255)

                                  tATCTCAGCAAAGTTGGCAGCCATTAATACACCTACCACCACTAAGGCTTGTGTCGTAACTGCACTTGTTACAACAGCGATTAACGGACTAAAGAACAGCGCTTATAGGAAACAACAACCTGTTACGATACTAGCAAATcc                                                                                                    <  2:244493/141‑1 (MQ=255)

                                    tctcAGCAAAGTTTGCAGCCATTAATACACCTACCACCACTAAGGCTGGTGTCGTAACTGCACTTGTTACAACAGCGATTAACGGACTAAAGAACAGCGCTTATAGGAAACAACAACCTGTTACGATACTAGCAAATCCTg                                                                                                  <  1:135345/141‑1 (MQ=255)

                                                                acCTACCACCACTAGGGCTGGTGTCGTAACTGCACTTGTTACAACAGCGATTAACGGACTAAAGAACAGCGCTTATAGGAAACAACAACCTGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGt                                                                      <  2:224906/141‑1 (MQ=255)

                                                                       accacTAAGGCTGGTGTCGTAACTGCACTTGTTACAACAGCGATTAACGGACTAAAGAACAGCGCTTATAGGAAACAACAACCTGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCg                                                               <  1:193205/141‑1 (MQ=255)

                                                                        ccacTAAGGCTGGTGTCGTAACTGCACTTGTTACAACAGCGATTAACGGACTAAAGAACAGCGCTTATAGGAAACAACAACCTGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGa                                                              <  1:264135/141‑1 (MQ=255)

                                                                            tcaGGCTGGTGTCGTAACTGCACTTGTTACAACAGCGATTAACGGACTAAAGAACAGCGCTTATAGGAAACAACAACCTGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGAtata                                                          <  1:88717/139‑1 (MQ=255)

                                                                                       tCGTAACTGCACTTGTTACAACAGCGATTAACGGACTGAAGAACAGCGCTTATAGAAAACAACAACATGTTACGATACTAGCAAATCCTGTTACAACACCTACAGCAACACCTGAAGTTGATTCGATATAACAAtttgatt                                               >  2:98222/1‑138 (MQ=255)

                                                                                         gTAACTGCACTTGTTACAACAGCGATTAAAGGACTAAAGAACAGCGCTTATAGGAAACAACAACCTGTTACGATACTAGCAAATCCTGTTCTAACACCTACAGCAACACCTAAAATTTAATCCATATAAAAAGTTGTTTTa                                             >  2:1595/1‑141 (MQ=255)

                                                                                              tGCACTTGTTACAACAGCGATTAACGGACTAAAGAACAGCGCTTATAGGAAACAACAACCTGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAAAACATGAAGTTGATTCGATATAGTCAGTCGTTGTAgttgt                                        >  2:257339/1‑141 (MQ=255)

                                                                                                 aCTTGTTACAACAGCGATTAACGGACTAAAGAACAGCGCTTATAGGAAACAACAACCTGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTcc                                     <  1:116591/141‑1 (MQ=255)

                                                                                                                            aaaGAACAGCGCTTATAGGAAACAACAACCTGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTaa          <  2:221084/141‑1 (MQ=255)

                                                                                                                             aaGAACAGGGCTTATAGTGAACAACAGCCTTTTACGATATTAGCAAATCCTGTTCTAGCACCTACAGCAACATCTGAAGTTGACTCGATATAAGAATTTGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTaaa         <  2:34375/141‑1 (MQ=255)

                                                                                                                                    gcgcTTATAGGAAACAACAACCTGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTATAGTTCTTCCACAAACTGATCCTCCGATCGTAGCTAAAGAAtata  >  2:92883/1‑138 (MQ=255)

AGGCTGGCACTGCAACTTCAAAACTTTTCCAGTTTATCTCAGCAAAGTTTGCAGCCATTAATACACCTACCACCACTAAGGCTGGTGTCGTAACTGCACTTGTTACAACAGCGATTAACGGACTAAAGAACAGCGCTAATAGGAAACAACAACCTGTTACGATACTAGCAAATCCTGTTCTAGCACCTACAGCAACACCTGAAGTTGATTCGATATAAGAAGTTGTTGTAGTTGTTCCAAAAACTGATCCTACAATCGTAGCTAAAGAATCTG  >  USA300TCH1516_ALE/2372614‑2372886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A