Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F36 I1 R1 22 37.6 1496218 97.1% 1452827 127.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,964,929 C→T E234E (GAG→GAA)  rhlB ← ATP‑dependent RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,964,9290CT100.0% 158.5 / NA 45E234E (GAG→GAA) rhlBATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/24);  total (21/24)

ACGATGACCATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGCTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCA  >  NC_000913/3964785‑3965074
                                                                                                                                                |                                                                                                                                                 
acGATGACCATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTtctt                                                                                                                                               <  1:116864/149‑1 (MQ=255)
  gATGACCATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTtcttct                                                                                                                                             <  1:69213/149‑1 (MQ=255)
  gATGACCATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTtcttct                                                                                                                                             >  2:69213/1‑149 (MQ=255)
  gATGACCATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTtcttct                                                                                                                                             >  3:64819/1‑149 (MQ=255)
                   aGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACACATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGt                                                                                                                            >  8:112575/1‑149 (MQ=255)
                              gATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCg                                                                                                                 <  4:146294/149‑1 (MQ=255)
                                      cacaACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGCGGCCCGTTTTCTGTTCCGGTTCCACt                                                                                                         >  2:152822/1‑149 (MQ=255)
                                           cGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAat                                                                                                    <  4:64819/149‑1 (MQ=255)
                                                       ttGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGCGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCAtt                                                                                        <  1:152822/149‑1 (MQ=255)
                                                            gAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCa                                                                                   >  4:87343/1‑149 (MQ=255)
                                                               aataatCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCt                                                                                >  8:58996/1‑149 (MQ=255)
                                                                       ctctGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAAc                                                                        >  6:76223/1‑149 (MQ=255)
                                                                               ggCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGtt                                                                 >  1:45937/1‑148 (MQ=255)
                                                                                gCCACTCTTCTTCGATCAGCGTTTGCAGCAAACACATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGtt                                                                 <  7:112575/147‑1 (MQ=255)
                                                                                 ccACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCAc                                                              <  1:100231/149‑1 (MQ=255)
                                                                                  cACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACg                                                             <  5:76223/149‑1 (MQ=255)
                                                                                       ttcttcGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTc                                                                            <  6:175523/129‑1 (MQ=255)
                                                                                       ttcttcGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTc                                                                            >  5:175523/1‑129 (MQ=255)
                                                                                       ttcttcGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCAc                                                              <  6:80881/143‑1 (MQ=255)
                                                                                       ttcttcGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCAc                                                              >  5:80881/1‑143 (MQ=255)
                                                                                         cttcGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCgg                                                      >  7:78941/1‑149 (MQ=255)
                                                                                          ttcGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTc                                                                <  7:168932/138‑1 (MQ=255)
                                                                                          ttcGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTc                                                                >  8:168932/1‑138 (MQ=255)
                                                                                          ttcGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCgg                                                      >  2:166563/1‑148 (MQ=255)
                                                                                             gATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCTTTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACg                                                  <  4:142938/149‑1 (MQ=255)
                                                                                                  gCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGc                                             <  5:122026/149‑1 (MQ=255)
                                                                                                  gCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGc                                             >  6:122026/1‑149 (MQ=255)
                                                                                                  gCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGc                                             >  2:51470/1‑149 (MQ=255)
                                                                                                   cGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCg                                            <  1:166563/149‑1 (MQ=255)
                                                                                                      ttGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTgg                                         <  1:51470/149‑1 (MQ=255)
                                                                                                          agcaAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGt                                                     <  4:131792/133‑1 (MQ=255)
                                                                                                          agcaAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGt                                                     >  3:131792/1‑133 (MQ=255)
                                                                                                          agcaAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGga                                     <  3:87343/149‑1 (MQ=255)
                                                                                                          agcaAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGga                                     <  2:45937/149‑1 (MQ=255)
                                                                                                               aCGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACa                                <  7:58996/149‑1 (MQ=255)
                                                                                                                     tttttCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGt                          >  5:163303/1‑149 (MQ=255)
                                                                                                                         tcttcGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCg                                            >  4:53669/1‑127 (MQ=255)
                                                                                                                         tcttcGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCg                                            <  3:53669/127‑1 (MQ=255)
                                                                                                                           ttcGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTc                                                                                                                        <  5:139019/49‑1 (MQ=255)
                                                                                                                           ttcGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTc                                                                                                                        >  6:139019/1‑49 (MQ=255)
                                                                                                                              gTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGgcgc                  <  8:78941/148‑1 (MQ=255)
                                                                                                                                       gTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAg        <  6:163303/149‑1 (MQ=255)
                                                                                                                                           aaaaGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGaaa                                               <  8:147897/106‑1 (MQ=255)
                                                                                                                                           aaaaGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGaaa                                               >  7:147897/1‑106 (MQ=255)
                                                                                                                                             aaGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCa  >  7:141519/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
ACGATGACCATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGCTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCA  >  NC_000913/3964785‑3965074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CACGATGACCATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGCTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCAT  >  NC_000913/3964784‑3965075
                                                                                                                                                 |                                                                                                                                                  
CACGATGACCATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTC                                                                                                                                               <  NB501016:228:HFK2FAFXY:1:21104:23277:3640/151‑1 (MQ=60)
  CGATGACCATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTT                                                                                                                                             <  NB501016:228:HFK2FAFXY:1:11212:14673:3081/151‑1 (MQ=60)
  CGATGACCATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTT                                                                                                                                             >  NB501016:228:HFK2FAFXY:2:11304:9839:10841/1‑151 (MQ=60)
                                                       GTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGCGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTG                                                                                        <  NB501016:228:HFK2FAFXY:1:21204:8853:8847/151‑1 (MQ=60)
                                                                              CTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTC                                                                                                                                                  <  NB501016:228:HFK2FAFXY:4:11603:14912:14058/70‑1 (MQ=60)
                                                                               TGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTC                                                                 >  NB501016:228:HFK2FAFXY:1:11204:3462:4690/1‑150 (MQ=60)
                                                                                GGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACACATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTC                                                                 <  NB501016:228:HFK2FAFXY:4:21411:25530:13005/149‑1 (MQ=60)
                                                                                 GCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACG                                                              <  NB501016:228:HFK2FAFXY:1:11310:11825:13192/151‑1 (MQ=60)
                                                                                  CCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGT                                                             <  NB501016:228:HFK2FAFXY:3:11604:18565:16013/151‑1 (MQ=60)
                                                                                       CTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACG                                                              >  NB501016:228:HFK2FAFXY:3:11606:23085:9539/1‑145 (MQ=60)
                                                                                       CTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCG                                                                            >  NB501016:228:HFK2FAFXY:3:21605:14690:18714/1‑131 (MQ=60)
                                                                                         TCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGT                                                      >  NB501016:228:HFK2FAFXY:4:11609:7568:18798/1‑151 (MQ=60)
                                                                                          CTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCA                                                                <  NB501016:228:HFK2FAFXY:4:21612:8750:12894/140‑1 (MQ=60)
                                                                                                  AGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCG                                             <  NB501016:228:HFK2FAFXY:3:21409:23278:14750/151‑1 (MQ=60)
                                                                                                   GCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGT                                            <  NB501016:228:HFK2FAFXY:1:21209:10967:2681/151‑1 (MQ=60)
                                                                                                      GTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGC                                         <  NB501016:228:HFK2FAFXY:1:11206:4505:1474/151‑1 (MQ=60)
                                                                                                          CAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAG                                     <  NB501016:228:HFK2FAFXY:2:21101:20450:4501/151‑1 (MQ=60)
                                                                                                          CAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTA                                                     >  NB501016:228:HFK2FAFXY:2:21209:15122:1494/1‑135 (MQ=60)
                                                                                                               AACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAG                                <  NB501016:228:HFK2FAFXY:4:11601:15035:4489/151‑1 (MQ=60)
                                                                                                                     TTTTTTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTT                          >  NB501016:228:HFK2FAFXY:3:21601:15688:11299/1‑151 (MQ=60)
                                                                                                                         TTCTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGT                                            <  NB501016:228:HFK2FAFXY:2:11211:6537:20329/129‑1 (MQ=60)
                                                                                                                           CTTCGTTAGAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCT                                                                                                                        <  NB501016:228:HFK2FAFXY:3:21504:10957:5110/51‑1 (MQ=60)
                                                                                                                                           GAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAG                                               >  NB501016:228:HFK2FAFXY:4:21603:7865:4498/1‑108 (MQ=60)
                                                                                                                                             AAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCAT  >  NB501016:228:HFK2FAFXY:4:21512:9155:9573/1‑151 (MQ=60)
                                                                                                                                                 |                                                                                                                                                  
CACGATGACCATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAGGGTAGAAAAGCTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCAT  >  NC_000913/3964784‑3965075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑