Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F4 I1 R1
|
17 |
37.4 |
1246952 |
98.2% |
1224506 |
142.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,176,112 |
G→A |
P241L (CCC→CTC) |
cpdA ← |
3',5' cAMP phosphodiesterase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,176,112 | 0 | G | A | 100.0%
| 137.6
/ NA
| 42 | P241L (CCC→CTC) | cpdA | 3',5' cAMP phosphodiesterase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (21/21); total (21/21) |
CTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGG > NC_000913/3175972‑3176254
|
cTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGAGCGCGATg < 5:74403/149‑1 (MQ=255)
tGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGAGCGCGATgg > 1:29223/1‑149 (MQ=255)
taaataaaGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGAGCGCGATGGTATCCAGCGTaaa < 3:3650/149‑1 (MQ=255)
aataaaGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAGt > 1:176155/1‑149 (MQ=255)
aataaaGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAGt > 2:128257/1‑149 (MQ=255)
ataaaGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAGtt > 2:178448/1‑149 (MQ=255)
aaaGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGAGCGCGAt < 3:123861/129‑1 (MQ=255)
aaaGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGAGCGCGAt > 4:123861/1‑129 (MQ=255)
agaagCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAGTTGGaa > 6:18393/1‑149 (MQ=255)
gTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGc > 8:32900/1‑149 (MQ=255)
aGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTaaa < 7:101719/148‑1 (MQ=255)
aGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTaaa < 1:128257/148‑1 (MQ=255)
gTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACt > 7:41469/1‑149 (MQ=255)
gCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGca < 2:29223/149‑1 (MQ=255)
ttCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGcaca > 7:60278/1‑148 (MQ=255)
tGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAgg > 8:110811/1‑149 (MQ=255)
caGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTc > 7:33016/2‑149 (MQ=255)
aaaCGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCCGCCGAGCGCGATGGTATCCAGCGTAAAGTGGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCcagcag < 3:19599/149‑1 (MQ=255)
cAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTc < 1:95439/148‑1 (MQ=255)
cAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTc > 2:95439/1‑148 (MQ=255)
aTGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCga < 7:32900/149‑1 (MQ=255)
aTGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCga < 8:41469/149‑1 (MQ=255)
cACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTc > 4:24550/1‑144 (MQ=255)
ccTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATcc < 3:24550/149‑1 (MQ=255)
ggtggtCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAAc < 7:132353/91‑1 (MQ=255)
ggtggtCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAAc > 8:132353/1‑91 (MQ=255)
tggtCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTcc < 2:176155/149‑1 (MQ=255)
aGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCtgat < 1:178448/148‑1 (MQ=255)
aTCAGCATGTAACTCGAGAGTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGAc > 7:8690/1‑149 (MQ=255)
aTCAGCATGTAACTCGAGAGTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGAc < 5:18393/149‑1 (MQ=255)
aTCAGCATGTAACTCGAGAGTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGAc < 8:60278/149‑1 (MQ=255)
tGTAACTCGAGAGTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCtgatga < 2:61346/135‑1 (MQ=255)
tGTAACTCGAGAGTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCtgatga > 1:61346/1‑135 (MQ=255)
aCTCGAGAGTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCaa < 8:8690/148‑1 (MQ=255)
tCGAGAGTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTa > 7:85940/1‑149 (MQ=255)
tCGAGAGTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTa > 3:48366/1‑149 (MQ=255)
gagaGTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTAtt > 2:64927/1‑149 (MQ=255)
gagaGTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTAt < 8:85940/148‑1 (MQ=255)
agagTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTAttt < 4:48366/149‑1 (MQ=255)
gagTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTAttt > 7:2678/1‑148 (MQ=255)
gCCAGCCGAGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGc < 8:28164/149‑1 (MQ=255)
aGCCGAGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCgg > 3:1973/1‑148 (MQ=255)
|
CTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGG > NC_000913/3175972‑3176254
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
CGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGA > NC_000913/3175963‑3176255
|
CGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGAG < NB501016:228:HFK2FAFXY:3:21405:5245:5729/151‑1 (MQ=60)
GCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGAGCGCGATGG < NB501016:228:HFK2FAFXY:3:11609:20328:10701/151‑1 (MQ=60)
CTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGAGCGCGATGGT > NB501016:228:HFK2FAFXY:1:11112:8696:7822/1‑151 (MQ=60)
GTAAATAAAGACGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAG < NB501016:228:HFK2FAFXY:2:11102:5593:17355/151‑1 (MQ=60)
AAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAGTT > NB501016:228:HFK2FAFXY:1:21311:15275:10493/1‑151 (MQ=60)
TAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGAGCGCGATG < NB501016:228:HFK2FAFXY:2:21303:8756:20070/131‑1 (MQ=60)
CAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAAC < NB501016:228:HFK2FAFXY:1:21204:9451:2618/150‑1 (MQ=60)
CAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAAC < NB501016:228:HFK2FAFXY:4:21504:13235:3989/150‑1 (MQ=60)
AGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTG > NB501016:228:HFK2FAFXY:4:11509:6917:6418/1‑151 (MQ=60)
CTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACAC > NB501016:228:HFK2FAFXY:4:11607:10547:5953/1‑150 (MQ=60)
GCAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCG > NB501016:228:HFK2FAFXY:4:11504:5629:14791/1‑151 (MQ=60)
GAAACGGGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCCGCCGAGCGCGATGGTATCCAGCGTAAAGTGGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGG < NB501016:228:HFK2FAFXY:2:11111:25019:7727/151‑1 (MQ=60)
CCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCC < NB501016:228:HFK2FAFXY:1:21102:13033:17962/151‑1 (MQ=60)
GATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAG < NB501016:228:HFK2FAFXY:4:11504:11225:13548/151‑1 (MQ=60)
GATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAG < NB501016:228:HFK2FAFXY:2:11208:15183:3283/151‑1 (MQ=60)
ACCTCGGGGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCA < NB501016:228:HFK2FAFXY:2:11201:5707:19174/151‑1 (MQ=60)
CGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACT < NB501016:228:HFK2FAFXY:4:21608:20958:19335/93‑1 (MQ=60)
CAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATG < NB501016:228:HFK2FAFXY:1:21312:15134:8711/150‑1 (MQ=60)
CATCAGCATGTAACTCGAGAGTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACC < NB501016:228:HFK2FAFXY:3:11409:6954:9118/151‑1 (MQ=60)
CATCAGCATGTAACTCGAGAGTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACC > NB501016:228:HFK2FAFXY:4:11405:13070:7505/1‑151 (MQ=60)
ATGTAACTCGAGAGTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAA > NB501016:228:HFK2FAFXY:1:11301:23478:5119/1‑137 (MQ=60)
CTCGAGAGTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTAT > NB501016:228:HFK2FAFXY:2:11302:22457:3288/1‑151 (MQ=60)
CTCGAGAGTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTAT > NB501016:228:HFK2FAFXY:4:21408:11930:11052/1‑151 (MQ=60)
AGAGTACGCCAGCCGAGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTG > NB501016:228:HFK2FAFXY:4:11402:2505:7705/1‑150 (MQ=60)
CAGCCGAGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGA > NB501016:228:HFK2FAFXY:2:11102:21707:1063/1‑150 (MQ=60)
CGAGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCG < NB501016:228:HFK2FAFXY:3:11410:21962:17866/122‑1 (MQ=60)
|
CGCGGCGAGCTGTTGAAACCGTGTAAATAAAGAAGCGTAGACATCAGTAGCCTTCTGAAGCGGTATCAGGTTGGAAACGTGTGTCCGCCAGGCGATGCACCTCGGTGGTCAGCGTGCCATCAGCATGTAACTCGAGAGTACGCCAGCCGGGCGCGATGGTATCCAGCGTAAAGTTGGAACAGTGCGGCTTAAACTGCACACAGGTCGACGGCGTTGCCAGCAGGCGGCGACCATTCCAGTCGAGATCCAGCTCCTGATGAATATGACCGCACAGCAAGTATTTGACGTGCGGA > NC_000913/3175963‑3176255
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
