Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1357	179.3	9500450	96.1%	9129932	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,738,433	T→C	F167L (TTT→CTT)	ydhC →	putative transport protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,738,433	0	T	C	98.4%	382.9 / ‑2.6	121	F167L (TTT→CTT)	ydhC	putative transport protein
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (70/49); total (71/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.52e-01

Only 100 of 123 total aligned reads displayed.

CCGCTGGTGGGTCTATCTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGTTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGGTCTGACCTTT  >  NC_000913/1738343‑1738522

ccGCTGGTGGGTCTATCTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCCCCCTGctt                                                                                         <  2:250957/93‑1 (MQ=255)

      gtggGTCTATCTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCAtt                                                                                   <  2:2827101/93‑1 (MQ=255)

       tggGTCTATCTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTa                                                                                  <  1:4744923/93‑1 (MQ=255)

          gTCTATCTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCg                                                                               <  1:4260162/93‑1 (MQ=255)

            cTATCTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCgtg                                                                             >  1:2778692/1‑93 (MQ=255)

             tATCTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCgtgg                                                                            <  2:1194350/93‑1 (MQ=255)

             tATCTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCgtgg                                                                            >  2:3275405/1‑93 (MQ=255)

             tATCTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCgtgg                                                                            >  2:3275404/1‑93 (MQ=255)

              aTCTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCgtggt                                                                           >  2:4018111/1‑93 (MQ=255)

              aTCTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCgtggt                                                                           <  1:4540621/93‑1 (MQ=255)

               tctcCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCgtggtg                                                                          >  2:4326207/1‑93 (MQ=255)

                ctcCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGc                                                                         <  1:694703/93‑1 (MQ=255)

                ctcCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGc                                                                         >  2:3164394/1‑93 (MQ=255)

                ctcCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGc                                                                         <  2:1064099/93‑1 (MQ=255)

                ctcCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGc                                                                         <  2:3080866/93‑1 (MQ=255)

                 tcCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGTTTGCCATTACCGTGGTGCt                                                                        >  2:4016544/1‑93 (MQ=255)

                 tcCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCt                                                                        >  2:676572/1‑93 (MQ=255)

                  ccGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTg                                                                       >  1:382669/1‑93 (MQ=255)

                    ggCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGAt                                                                     >  1:4052340/1‑93 (MQ=255)

                     gCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGAtt                                                                    >  1:294316/1‑93 (MQ=255)

                      cACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTc                                                                   <  2:2934221/93‑1 (MQ=255)

                       aCTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCt                                                                  >  1:791963/1‑93 (MQ=255)

                          ggCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGTTTGCCATTACCGTGGTGCTGATTCTGcc                                                               <  1:4043995/93‑1 (MQ=255)

                          ggCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGcc                                                               >  2:4380947/1‑93 (MQ=255)

                          ggCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGcc                                                               <  1:493069/93‑1 (MQ=255)

                          ggCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGcc                                                               <  1:3605603/93‑1 (MQ=255)

                               ctctGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTAttt                                                          >  2:1188133/1‑93 (MQ=255)

                                 ctGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGTCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTAttttt                                                        >  1:1578922/1‑92 (MQ=255)

                                 ctGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTc                                                        >  1:1578923/1‑93 (MQ=255)

                                  tGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCAATTTTCt                                                       <  1:741069/93‑1 (MQ=255)

                                     tAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGc                                                    >  2:1347264/1‑93 (MQ=255)

                                        gAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCa                                                 <  2:1029211/93‑1 (MQ=255)

                                        gAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCa                                                 >  1:67198/1‑93 (MQ=255)

                                        gAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCa                                                 >  1:2636588/1‑93 (MQ=255)

                                         aaGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCaa                                                >  1:3531747/1‑93 (MQ=255)

                                           gctggctgCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAAc                                              <  2:620113/93‑1 (MQ=255)

                                           gctggctgCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAAc                                              >  2:2773527/1‑93 (MQ=255)

                                              ggctgCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCa                                           >  1:941829/1‑93 (MQ=255)

                                              ggctgCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCa                                           <  2:2558726/93‑1 (MQ=255)

                                               gctgctGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCac                                          <  2:1909958/93‑1 (MQ=255)

                                                ctgctgGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCacg                                         <  2:473321/93‑1 (MQ=255)

                                                ctgctgGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCacg                                         <  2:473320/93‑1 (MQ=255)

                                                 tgctgGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCacga                                        <  1:1031781/93‑1 (MQ=255)

                                                 tgctgGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCacga                                        <  1:1667219/93‑1 (MQ=255)

                                                 tgctgGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCacga                                        >  2:1124356/1‑93 (MQ=255)

                                                 tgctgGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCacga                                        >  1:2709083/1‑93 (MQ=255)

                                                  gctgGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCacgac                                       >  1:4236815/1‑93 (MQ=255)

                                                   ctgGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCacgacg                                      <  2:3181341/93‑1 (MQ=255)

                                                    tgGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCAcgacga                                     >  1:4542774/1‑93 (MQ=255)

                                                     ggTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGaa                                    >  1:2098577/1‑93 (MQ=255)

                                                     ggTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCAACGACGaa                                    >  1:3617905/1‑93 (MQ=255)

                                                      gTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAg                                   >  1:2768875/1‑93 (MQ=255)

                                                       tCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAgg                                  <  1:965434/93‑1 (MQ=255)

                                                       gccATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAgg                                  <  1:701895/92‑1 (MQ=255)

                                                          aTTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGccc                               >  1:2632937/1‑93 (MQ=255)

                                                          aTTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGccc                               <  1:3566140/93‑1 (MQ=255)

                                                          aTTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGccc                               >  1:207843/1‑93 (MQ=255)

                                                           tttttCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGTCTCAAACCCACGACGAAGGCCCg                              >  2:2606657/1‑93 (MQ=255)

                                                           tttttCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCg                              <  2:1993434/93‑1 (MQ=255)

                                                           tttttCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCg                              <  1:4489755/93‑1 (MQ=255)

                                                           tttttCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCg                              <  2:1620795/93‑1 (MQ=255)

                                                            ttttCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGt                             >  2:3244449/1‑93 (MQ=255)

                                                              ttCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTaa                           <  1:1024971/93‑1 (MQ=255)

                                                               tCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAAc                          >  2:557403/1‑93 (MQ=255)

                                                               tCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAAc                          >  1:1951326/1‑93 (MQ=255)

                                                               tCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAAc                          >  2:4334215/1‑93 (MQ=255)

                                                               tCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGACCGCAAc                          >  1:1951325/1‑93 (MQ=255)

                                                                   ggCAGGCGATTTTCGCCACCCTGCTTGCGATTACCGTGTTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATa                      >  2:4722211/1‑93 (MQ=255)

                                                                   ggCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATa                      <  2:4673421/93‑1 (MQ=255)

                                                                     cAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAAGCATAGt                    >  1:1994956/1‑93 (MQ=255)

                                                                      aGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTc                   <  2:3216745/93‑1 (MQ=255)

                                                                      aGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTc                   >  2:1348553/1‑93 (MQ=255)

                                                                       ggCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCa                  >  2:3590960/1‑93 (MQ=255)

                                                                       ggCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCa                  <  2:1891286/93‑1 (MQ=255)

                                                                       ggCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCa                  <  2:4258791/93‑1 (MQ=255)

                                                                       ggCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCa                  >  1:66179/1‑93 (MQ=255)

                                                                       ggCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCa                  >  1:878244/1‑93 (MQ=255)

                                                                       ggCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCa                  <  1:1546834/93‑1 (MQ=255)

                                                                       ggCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCa                  >  2:1978535/1‑93 (MQ=255)

                                                                          gATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGa               <  2:4136515/93‑1 (MQ=255)

                                                                          gATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGa               >  2:2581045/1‑93 (MQ=255)

                                                                          gATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGa               >  1:2058741/1‑93 (MQ=255)

                                                                           aTTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGAt              >  1:3776601/1‑93 (MQ=255)

                                                                           aTTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGAt              >  1:3975157/1‑93 (MQ=255)

                                                                           aTTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGAt              >  2:3075631/1‑93 (MQ=255)

                                                                            ttttCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATg             <  1:1059618/93‑1 (MQ=255)

                                                                                cGCCACCCTTCTGGCCATGACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGGTCt         <  2:2433549/93‑1 (MQ=255)

                                                                                cGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGGTCt         <  2:1775957/93‑1 (MQ=255)

                                                                                 gCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGGTCTg        >  1:4089898/1‑93 (MQ=255)

                                                                                 gCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGGTCTg        >  2:1600891/1‑93 (MQ=255)

                                                                                  ccACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGGTCTGa       <  2:840044/93‑1 (MQ=255)

                                                                                   cccccTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGGTCTGAc      <  1:1016920/91‑1 (MQ=255)

                                                                                    aCCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGGTCTGAcc     >  1:4595328/1‑93 (MQ=255)

                                                                                    aCCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGGTCTGAcc     >  1:4200311/1‑93 (MQ=255)

                                                                                     cccTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGGTCTGACCt    >  2:1116303/1‑93 (MQ=255)

                                                                                     cccTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGGTCTGACCt    >  2:4270440/1‑93 (MQ=255)

                                                                                      ccTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGGTCTGACCtt   >  2:310004/1‑93 (MQ=255)

                                                                                       cTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGGTCTGACCttt  >  1:4609611/1‑93 (MQ=255)

                                                                                       cTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGGTCTGACCttt  <  1:1034208/93‑1 (MQ=255)

                                                                                       cTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGGTCTGACCttt  >  1:2915304/1‑93 (MQ=255)

CCGCTGGTGGGTCTATCTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGTTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGGTCTGACCTTT  >  NC_000913/1738343‑1738522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTGGTGGGTCTATCTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGTTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGGTCTGACCTTTACC  >  NC_000913/1738346‑1738525

CTGGTGGGTATATCTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCA                                                                                        <  SRR4302544.18169/94‑1 (MQ=60)

    TGGGTCTATCTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTAC                                                                                    <  SRR4302544.4791677/94‑1 (MQ=60)

       GTCTATCTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGT                                                                                 <  SRR4302544.4303605/94‑1 (MQ=60)

        TCTATCTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTG                                                                                >  SRR4302544.2805423/1‑94 (MQ=60)

           ATCTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTG                                                                             <  SRR4302544.4586002/94‑1 (MQ=60)

             CTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCT                                                                           <  SRR4302544.701627/94‑1 (MQ=60)

              TCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTG                                                                          >  SRR4302544.386563/1‑94 (MQ=60)

                CGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGAT                                                                        >  SRR4302544.4092693/1‑94 (MQ=60)

                CGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGAT                                                                        <  SRR4302544.471918/94‑1 (MQ=60)

                 GGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATT                                                                       >  SRR4302544.297362/1‑94 (MQ=60)

                   CACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCT                                                                     >  SRR4302544.799632/1‑94 (MQ=60)

                    ACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTG                                                                    <  SRR4302544.210632/94‑1 (MQ=60)

                       GGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGTTTGCCATTACCGTGGTGCTGATTCTGCCT                                                                 <  SRR4302544.4084275/94‑1 (MQ=60)

                       GGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCT                                                                 <  SRR4302544.3640609/94‑1 (MQ=60)

                       GGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCT                                                                 <  SRR4302544.497918/94‑1 (MQ=60)

                        NNNCNTCNGNTANGAAGNTGNNNNCTGGTCCATTTTTCCTGGCAGGCNATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTA                                                                <  SRR4302544.3462316/94‑1 (MQ=60)

                            CTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTT                                                            <  SRR4302544.336777/94‑1 (MQ=60)

                             TCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGTCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTT                                                           >  SRR4302544.1594529/1‑94 (MQ=60)

                             TCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTC                                                           >  SRR4302544.1594530/1‑94 (MQ=60)

                               TGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCAATTTTCTG                                                         <  SRR4302544.748322/94‑1 (MQ=60)

                                    GGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCA                                                    >  SRR4302544.2661861/1‑94 (MQ=60)

                                    GGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCA                                                    >  SRR4302544.67869/1‑94 (MQ=60)

                                     GAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAA                                                   >  SRR4302544.3565529/1‑94 (MQ=60)

                                          TGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCA                                              <  SRR4302544.4119574/94‑1 (MQ=60)

                                          TGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCA                                              >  SRR4302544.951103/1‑94 (MQ=60)

                                             CTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGTCATTACCGTGGTGCTGATTCTGCCTATTT‑‑TGGCTCAAACCCACTACG                                         >  SRR4302544.2735135/1‑94 (MQ=60)

                                             CTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGA                                           >  SRR4302544.2735134/1‑94 (MQ=60)

                                             CTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGA                                           >  SRR4302544.3821608/1‑94 (MQ=60)

                                              TGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGAC                                          >  SRR4302544.4279998/1‑94 (MQ=60)

                                              TGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGAC                                          <  SRR4302544.1683638/94‑1 (MQ=60)

                                              TGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGAC                                          <  SRR4302544.1042050/94‑1 (MQ=60)

                                               GCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACG                                         >  SRR4302544.1149480/1‑94 (MQ=60)

                                                CTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGA                                        >  SRR4302544.4588166/1‑94 (MQ=60)

                                                 TGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAA                                       >  SRR4302544.2118773/1‑94 (MQ=60)

                                                 TGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCAACGACGAA                                       >  SRR4302544.3653827/1‑94 (MQ=60)

                                                  GGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAG                                      >  SRR4302544.2795517/1‑94 (MQ=60)

                                                    TCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGC                                    >  SRR4302544.4409287/1‑94 (MQ=60)

                                                    TCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGC                                    <  SRR4302544.974905/94‑1 (MQ=60)

                                                    GCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGC                                    <  SRR4302544.708883/94‑1 (MQ=60)

                                                     CCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCC                                   >  SRR4302544.800041/1‑94 (MQ=60)

                                                      CATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCC                                  >  SRR4302544.2658173/1‑94 (MQ=60)

                                                      CATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCC                                  >  SRR4302544.209974/1‑94 (MQ=60)

                                                       ATTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCG                                 <  SRR4302544.3600330/94‑1 (MQ=60)

                                                        TTTTTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGT                                <  SRR4302544.4534834/94‑1 (MQ=60)

                                                           TTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAAC                             <  SRR4302544.1035204/94‑1 (MQ=60)

                                                           TTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAAC                             >  SRR4302544.1970244/1‑94 (MQ=60)

                                                           TTCCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGACCGCAAC                             >  SRR4302544.1970243/1‑94 (MQ=60)

                                                             CCTGGCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAA                           >  SRR4302544.696175/1‑94 (MQ=60)

                                                                 GCAGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAAGCATAGT                       >  SRR4302544.2014325/1‑94 (MQ=60)

                                                                   AGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCA                     >  SRR4302544.66841/1‑94 (MQ=60)

                                                                   AGGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCA                     >  SRR4302544.886954/1‑94 (MQ=60)

                                                                    GGCGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAG                    <  SRR4302544.1562238/94‑1 (MQ=60)

                                                                      CGATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGA                  >  SRR4302544.2078544/1‑94 (MQ=60)

                                                                       GATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGAT                 >  SRR4302544.3814025/1‑94 (MQ=60)

                                                                       GATTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGAT                 >  SRR4302544.4014713/1‑94 (MQ=60)

                                                                         TTTTCGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGG               <  SRR4302544.1070142/94‑1 (MQ=60)

                                                                             CGCCACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGGTCTG           >  SRR4302544.4130576/1‑94 (MQ=60)

                                                                                CCCCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGGTCTGACC        <  SRR4302544.1027067/94‑1 (MQ=60)

                                                                                CACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGGTCTGACC        >  SRR4302544.4641034/1‑94 (MQ=60)

                                                                                CACCCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGGTCTGACC        >  SRR4302544.4242235/1‑94 (MQ=60)

                                                                                   CCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGGTCTGACCTTT     >  SRR4302544.4655396/1‑94 (MQ=60)

                                                                                   CCTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGGTCTGACCTTT     >  SRR4302544.2943337/1‑94 (MQ=60)

                                                                                    CTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGGTCTGACCTTTA    <  SRR4302544.1044494/94‑1 (MQ=60)

                                                                                    CTGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGGTCTGACCTTTA    >  SRR4302544.3194550/1‑94 (MQ=60)

                                                                                     TGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGGTCTGACCTTTAC   >  SRR4302544.1194673/1‑94 (MQ=60)

                                                                                     TGCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGGTCTGACCTTTAC   >  SRR4302544.2096208/1‑94 (MQ=60)

                                                                                      GCTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGGTCTGACCTTTACC  <  SRR4302544.3879361/94‑1 (MQ=60)

CTGGTGGGTCTATCTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGTTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACGAAGGCCCGTAACAATAGTCAGGATGGTCTGACCTTTACC  >  NC_000913/1738346‑1738525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`