Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,049,149 G→A G75S (GGC→AGC)  yihI → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,049,1490GA98.5% 937.4 / ‑0.6 266G75S (GGC→AGC) yihIhypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (3/1);  new base A (130/132);  total (133/133)
Fisher's exact test for biased strand distribution p-value = 6.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.42e-01

Only 100 of 269 total aligned reads displayed.

CGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTATTCCATTGGGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACG  >  NC_000913/4049059‑4049238
                                                                                          |                                                                                         
  cgcAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGt                                                                                       <  2:190113/93‑1 (MQ=255)
  cgcAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGACCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGt                                                                                       <  1:485020/93‑1 (MQ=255)
   gcAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTg                                                                                      >  2:312123/1‑93 (MQ=255)
    cAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGa                                                                                     >  1:624011/1‑93 (MQ=255)
     aGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGAc                                                                                    <  1:2755814/93‑1 (MQ=255)
     aGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGAc                                                                                    <  1:3903873/93‑1 (MQ=255)
      gcgggcggAAACACCACGTCAGGCAGCAAAGGCCTGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACt                                                                                   <  1:3809782/93‑1 (MQ=255)
      gcgggcggAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTATTCCATTGGGCGTGACt                                                                                   >  2:3894321/1‑93 (MQ=255)
        ggtcggAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGa                                                                                 <  1:2329539/90‑1 (MQ=255)
        gggcggAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGa                                                                                 >  2:3925015/1‑93 (MQ=255)
         ggcggAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGCATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGaa                                                                                >  1:977740/1‑93 (MQ=255)
          gcggAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGaaa                                                                               <  1:1972564/93‑1 (MQ=255)
          gcggAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGaaa                                                                               <  2:1280244/93‑1 (MQ=255)
           cggAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGaaaa                                                                              <  1:2990866/93‑1 (MQ=255)
            ggAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGaaaaa                                                                             >  2:2430490/1‑93 (MQ=255)
             gAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAg                                                                            >  2:1954058/1‑93 (MQ=255)
               aaCACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTc                                                                          >  1:363786/1‑93 (MQ=255)
               aaCACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTc                                                                          >  2:4107056/1‑93 (MQ=255)
               aaCACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTc                                                                          >  2:1214247/1‑93 (MQ=255)
                  accacGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCAcc                                                                       <  2:4094470/93‑1 (MQ=255)
                  accacGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCAcc                                                                       <  1:2401322/93‑1 (MQ=255)
                       gTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACa                                                                  <  2:2747781/93‑1 (MQ=255)
                        tCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAg                                                                 >  2:1684143/1‑93 (MQ=255)
                        tCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAg                                                                 <  1:631282/93‑1 (MQ=255)
                         cAGGCAGCAAAGGCCAGAACGCATCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGc                                                                <  2:2580365/93‑1 (MQ=255)
                           ggCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGcac                                                              >  1:306110/1‑93 (MQ=255)
                           ggCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGcac                                                              >  2:1424569/1‑93 (MQ=255)
                                     gCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAaga                                                    <  1:3950068/93‑1 (MQ=255)
                                      ccAGAACGCACCAAAAGATCCACGTCTTGGCAGTAAAACACCTGTTCCATTGAGCGTGACTGCAAAAGTCACCAAACAGCACCCACAGAagag                                                   >  2:1156256/1‑93 (MQ=255)
                                       cAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGt                                                  >  1:3158329/1‑93 (MQ=255)
                                       cAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGt                                                  <  1:3552091/93‑1 (MQ=255)
                                       cAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGt                                                  >  1:4188974/1‑93 (MQ=255)
                                        aGAACGCACCAAAAGATCCGCGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAagagcg                                                 >  1:1728400/1‑91 (MQ=255)
                                        aGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGACAAAGTCACCAAACAGCCCCAACCGAagaggg                                                 >  1:1355952/1‑91 (MQ=255)
                                        aGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTg                                                 >  1:1279293/1‑93 (MQ=255)
                                        aGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTg                                                 >  1:2298939/1‑93 (MQ=255)
                                         gAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCATCAAACAGCACAAACCGAAGAGTga                                                >  2:4338148/1‑93 (MQ=255)
                                         gAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTga                                                <  1:2312438/93‑1 (MQ=255)
                                         gAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTga                                                >  2:3434341/1‑93 (MQ=255)
                                          aaCGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTgag                                               >  1:2018786/1‑93 (MQ=255)
                                             gCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGaaa                                            >  2:4555093/1‑93 (MQ=255)
                                              cACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAAc                                           <  1:952741/93‑1 (MQ=255)
                                               aCCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAAcc                                          <  2:3000215/93‑1 (MQ=255)
                                               aCCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAAcc                                          >  1:2255014/1‑93 (MQ=255)
                                               aCCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAAcc                                          >  1:2502773/1‑93 (MQ=255)
                                               aCCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAAcc                                          >  2:3413158/1‑93 (MQ=255)
                                               aCCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAAcc                                          >  2:3522933/1‑93 (MQ=255)
                                               aCCAAAAGATCCACGTATTGGCAGTAAAACCCCTATTCCATTGGGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAAcc                                          >  1:1591582/1‑93 (MQ=255)
                                                ccAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCt                                         >  1:3686985/1‑93 (MQ=255)
                                                ccAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCt                                         <  1:4542222/93‑1 (MQ=255)
                                                ccAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCt                                         <  1:1224256/93‑1 (MQ=255)
                                                ccAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCt                                         >  1:3332857/1‑93 (MQ=255)
                                                ccAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCt                                         >  1:1809380/1‑93 (MQ=255)
                                                 cAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTa                                        >  1:3776505/1‑93 (MQ=255)
                                                 cAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTa                                        >  2:524725/1‑93 (MQ=255)
                                                  aaaaGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTAt                                       <  1:4048549/93‑1 (MQ=255)
                                                  aaaaGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTAt                                       <  1:4046791/93‑1 (MQ=255)
                                                   aaaGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATg                                      <  1:2948106/93‑1 (MQ=255)
                                                   aaaGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATg                                      <  1:2828535/93‑1 (MQ=255)
                                                    aaGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGc                                     <  2:1581769/93‑1 (MQ=255)
                                                    aaGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGGCACCAAACAGCACAAAACGAAGAGTGAGAACCCTACGc                                     >  1:1110746/1‑93 (MQ=255)
                                                     aGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCt                                    <  2:3832125/93‑1 (MQ=255)
                                                     aGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCt                                    <  1:2105326/93‑1 (MQ=255)
                                                      gATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCtt                                   <  1:1432529/93‑1 (MQ=255)
                                                         ccACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCa                                >  2:3488538/1‑93 (MQ=255)
                                                         ccACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCa                                >  2:4445335/1‑93 (MQ=255)
                                                         ccACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCa                                <  2:1067120/93‑1 (MQ=255)
                                                         ccACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCa                                <  2:3610428/93‑1 (MQ=255)
                                                             gTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGc                            <  2:4542039/93‑1 (MQ=255)
                                                             gTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGc                            >  2:85492/1‑93 (MQ=255)
                                                              tATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCa                           >  1:1078517/1‑93 (MQ=255)
                                                              tATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCa                           <  1:4635548/93‑1 (MQ=255)
                                                                ttGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAgg                         >  1:2148999/1‑93 (MQ=255)
                                                                ttGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAgg                         >  1:575203/1‑93 (MQ=255)
                                                                ttGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAgg                         >  1:4398310/1‑93 (MQ=255)
                                                                 tGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGc                        >  2:3069504/1‑93 (MQ=255)
                                                                  ggCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCg                       <  1:3271772/93‑1 (MQ=255)
                                                                   gCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCgg                      <  2:4796/93‑1 (MQ=255)
                                                                     aGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAg                    >  2:4189632/1‑93 (MQ=255)
                                                                     aGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAg                    >  1:434157/1‑93 (MQ=255)
                                                                      gTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGt                   <  1:2539842/93‑1 (MQ=255)
                                                                       tAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGtt                  >  1:4131178/1‑93 (MQ=255)
                                                                          aaCCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGa               >  2:2069984/1‑93 (MQ=255)
                                                                          aaCCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGa               >  2:401793/1‑93 (MQ=255)
                                                                           aCCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGaa              >  2:1541715/1‑93 (MQ=255)
                                                                           aCCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGaa              <  1:1255256/93‑1 (MQ=255)
                                                                            ccccTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAAt             >  2:4661455/1‑93 (MQ=255)
                                                                             cccTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAAtt            <  1:2629329/93‑1 (MQ=255)
                                                                             cccTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAAtt            >  2:1821428/1‑93 (MQ=255)
                                                                             cccTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAAtt            >  2:62854/1‑93 (MQ=255)
                                                                              ccTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTa           >  1:470545/1‑93 (MQ=255)
                                                                              ccTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTa           >  1:898202/1‑93 (MQ=255)
                                                                              ccTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTa           <  1:2675875/93‑1 (MQ=255)
                                                                              ccTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGGTGGAATTa           >  1:1009462/1‑93 (MQ=255)
                                                                                  ttCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTgg       <  1:2836050/93‑1 (MQ=255)
                                                                                    ccATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGaa     <  2:3979084/93‑1 (MQ=255)
                                                                                    ccATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGaa     <  2:2947786/93‑1 (MQ=255)
                                                                                     cATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGaaa    <  2:4503479/93‑1 (MQ=255)
                                                                                     cATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGaaa    <  2:1861794/93‑1 (MQ=255)
                                                                                       ttGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGCTGGAATTACTGGAAACg  >  2:3757922/1‑93 (MQ=255)
                                                                                          |                                                                                         
CGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTATTCCATTGGGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACG  >  NC_000913/4049059‑4049238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTATTCCATTGGGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACGGAT  >  NC_000913/4049056‑4049241
                                                                                             |                                                                                            
AGCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCCACAGATCCACGTATTGGCAGTACAACCCCTGTTCCATTGC                                                                                              >  SRR4302544.1997625/1‑94 (MQ=60)
AGCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGA                                                                                              <  SRR4302544.2288692/94‑1 (MQ=60)
AGCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGA                                                                                              <  SRR4302544.1928009/94‑1 (MQ=60)
 GCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAG                                                                                             <  SRR4302544.4463713/94‑1 (MQ=60)
 GCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAG                                                                                             <  SRR4302544.385113/94‑1 (MQ=60)
 GCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAG                                                                                             <  SRR4302544.896788/94‑1 (MQ=60)
 GCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAG                                                                                             <  SRR4302544.1867219/94‑1 (MQ=60)
  CCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGC                                                                                            <  SRR4302544.788647/94‑1 (MQ=60)
   CGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCG                                                                                           <  SRR4302544.1301745/94‑1 (MQ=60)
     CGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCATAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTG                                                                                         <  SRR4302544.1971076/94‑1 (MQ=60)
     CGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTG                                                                                         <  SRR4302544.2605632/94‑1 (MQ=60)
     CGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGACCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTG                                                                                         <  SRR4302544.489768/94‑1 (MQ=60)
      GCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGC                                                                                        >  SRR4302544.1728799/1‑94 (MQ=60)
      GCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGA                                                                                        >  SRR4302544.630138/1‑94 (MQ=60)
       CAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGAC                                                                                       <  SRR4302544.2151697/94‑1 (MQ=60)
        AGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACT                                                                                      <  SRR4302544.2854740/94‑1 (MQ=60)
        AGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACT                                                                                      <  SRR4302544.3942657/94‑1 (MQ=60)
        AGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACT                                                                                      <  SRR4302544.2782338/94‑1 (MQ=60)
         GCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCTGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTG                                                                                     <  SRR4302544.3847536/94‑1 (MQ=60)
         GCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTG                                                                                     >  SRR4302544.2489952/1‑94 (MQ=60)
           GGTCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAA                                                                                   <  SRR4302544.2351625/94‑1 (MQ=60)
           GGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAA                                                                                   >  SRR4302544.987321/1‑94 (MQ=60)
           GGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAA                                                                                   >  SRR4302544.3979095/1‑94 (MQ=60)
           GGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGCATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAA                                                                                   >  SRR4302544.987322/1‑94 (MQ=60)
            GGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAA                                                                                  <  SRR4302544.4194795/94‑1 (MQ=60)
             GCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAA                                                                                 <  SRR4302544.1991734/94‑1 (MQ=60)
              CGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAA                                                                                <  SRR4302544.3019545/94‑1 (MQ=60)
                 AAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTC                                                                             >  SRR4302544.367509/1‑94 (MQ=60)
                  AACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCA                                                                            >  SRR4302544.2305407/1‑94 (MQ=60)
                     ACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCA                                                                         <  SRR4302544.2424106/94‑1 (MQ=60)
                       CACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAA                                                                       >  SRR4302544.2005309/1‑94 (MQ=60)
                         CGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCCCGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACA                                                                     <  SRR4302544.427804/94‑1 (MQ=60)
                           TCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGC                                                                   >  SRR4302544.4088263/1‑94 (MQ=60)
                           TCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGC                                                                   <  SRR4302544.637501/94‑1 (MQ=60)
                           TCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGC                                                                   >  SRR4302544.4088264/1‑94 (MQ=60)
                             AGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCAC                                                                 <  SRR4302544.524978/94‑1 (MQ=60)
                             AGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCAC                                                                 >  SRR4302544.309283/1‑94 (MQ=60)
                             AGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCAA                                                                 <  SRR4302544.1162360/94‑1 (MQ=60)
                                CAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAA                                                              >  SRR4302544.1240329/1‑94 (MQ=60)
                                   CAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCG                                                           <  SRR4302544.476908/94‑1 (MQ=60)
                                   CAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCG                                                           >  SRR4302544.4622996/1‑94 (MQ=60)
                                    AAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGA                                                          >  SRR4302544.2403094/1‑94 (MQ=60)
                                    AAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGA                                                          <  SRR4302544.1668469/94‑1 (MQ=60)
                                     AAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAA                                                         <  SRR4302544.3645963/94‑1 (MQ=60)
                                      AGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAG                                                        <  SRR4302544.1112661/94‑1 (MQ=60)
                                       GGCCAGAACGCACCAAAAGAGCCACGTATTGGCAGTAAAACGCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGA                                                       <  SRR4302544.3739440/94‑1 (MQ=60)
                                        GCCAGAACGCACCTACAGATCCGCGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAG                                                      <  SRR4302544.4268657/94‑1 (MQ=60)
                                        GCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAG                                                      <  SRR4302544.3989324/94‑1 (MQ=60)
                                        GCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAG                                                      <  SRR4302544.2810357/94‑1 (MQ=60)
                                         CCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGT                                                     <  SRR4302544.3105123/94‑1 (MQ=60)
                                         CCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGT                                                     >  SRR4302544.3188368/1‑94 (MQ=60)
                                         CCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGT                                                     >  SRR4302544.4230465/1‑94 (MQ=60)
                                          CAGAACGCACCAAAAGATCCGCGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGCG                                                    >  SRR4302544.1745347/1‑94 (MQ=60)
                                          CAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGACAAAGTCACCAAACAGCCCCAACCGAAGAGGG                                                    >  SRR4302544.1369402/1‑94 (MQ=60)
                                          CAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTG                                                    <  SRR4302544.3586111/94‑1 (MQ=60)
                                          CAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTG                                                    >  SRR4302544.2320635/1‑94 (MQ=60)
                                          CAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTG                                                    <  SRR4302544.1602698/94‑1 (MQ=60)
                                          CAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTG                                                    >  SRR4302544.1291896/1‑94 (MQ=60)
                                           CGAACGCACCAAAAGATCCACGTATTGGCAATAAAACCCCTGTGCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGA                                                   <  SRR4302544.58662/94‑1 (MQ=60)
                                           AGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGA                                                   >  SRR4302544.407985/1‑94 (MQ=60)
                                           AGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGA                                                   <  SRR4302544.3336307/94‑1 (MQ=60)
                                           AGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGA                                                   <  SRR4302544.2547033/94‑1 (MQ=60)
                                           AGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGA                                                   <  SRR4302544.1283278/94‑1 (MQ=60)
                                           AGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGA                                                   <  SRR4302544.1943614/94‑1 (MQ=60)
                                            GAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAG                                                  <  SRR4302544.2334311/94‑1 (MQ=60)
                                            GAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAG                                                  <  SRR4302544.1572488/94‑1 (MQ=60)
                                            GAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAG                                                  >  SRR4302544.2038385/1‑94 (MQ=60)
                                               CGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAA                                               >  SRR4302544.1124807/1‑94 (MQ=60)
                                               CGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAA                                               >  SRR4302544.2152130/1‑94 (MQ=60)
                                               CGCACCAAAAGATCCACGTATTGGC‑GTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAAC                                              <  SRR4302544.3069439/94‑1 (MQ=60)
                                                GCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAAC                                              <  SRR4302544.3069438/94‑1 (MQ=60)
                                                 CACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACC                                             >  SRR4302544.2276223/1‑94 (MQ=60)
                                                 CACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACC                                             <  SRR4302544.549536/94‑1 (MQ=60)
                                                 CACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACC                                             >  SRR4302544.2276224/1‑94 (MQ=60)
                                                 CACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACC                                             <  SRR4302544.962111/94‑1 (MQ=60)
                                                 CACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACC                                             >  SRR4302544.2526770/1‑94 (MQ=60)
                                                 CACCAAAAGATCCACGTATTGGCAGTAAAACCCCTATTCCATTGGGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACC                                             >  SRR4302544.1607272/1‑94 (MQ=60)
                                                  ACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCT                                            >  SRR4302544.1827059/1‑94 (MQ=60)
                                                  ACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCT                                            >  SRR4302544.3723740/1‑94 (MQ=60)
                                                  ACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCT                                            <  SRR4302544.2322815/94‑1 (MQ=60)
                                                  ACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCT                                            >  SRR4302544.1392297/1‑94 (MQ=60)
                                                  ACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCT                                            >  SRR4302544.3364597/1‑94 (MQ=60)
                                                   CCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTA                                           <  SRR4302544.1236366/94‑1 (MQ=60)
                                                   CCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTA                                           >  SRR4302544.3813929/1‑94 (MQ=60)
                                                   CCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTA                                           <  SRR4302544.4587611/94‑1 (MQ=60)
                                                    CAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTAT                                          >  SRR4302544.727620/1‑94 (MQ=60)
                                                     AAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATG                                         <  SRR4302544.4088873/94‑1 (MQ=60)
                                                     AAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATG                                         <  SRR4302544.4087101/94‑1 (MQ=60)
                                                     AAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATG                                         <  SRR4302544.102509/94‑1 (MQ=60)
                                                      AAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGC                                        <  SRR4302544.2855861/94‑1 (MQ=60)
                                                      AAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGC                                        <  SRR4302544.2976422/94‑1 (MQ=60)
                                                      AAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGGCACCAAACAGCACAAAACGAAGAGTGAGAACCCTACGC                                        >  SRR4302544.1121823/1‑94 (MQ=60)
                                                       AAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAGGAGTGAGAAACCTATGCT                                       >  SRR4302544.2701952/1‑94 (MQ=60)
                                                       AAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCT                                       >  SRR4302544.4211084/1‑94 (MQ=60)
                                                        AGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTT                                      <  SRR4302544.2921333/94‑1 (MQ=60)
                                                        AGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTT                                      <  SRR4302544.2125584/94‑1 (MQ=60)
                                                        AGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTT                                      >  SRR4302544.3207736/1‑94 (MQ=60)
                                                         GATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTT                                     <  SRR4302544.1446784/94‑1 (MQ=60)
                                                         GATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTT                                     <  SRR4302544.3832721/94‑1 (MQ=60)
                                                          ATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTC                                    >  SRR4302544.3934536/1‑94 (MQ=60)
                                                          ATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTC                                    <  SRR4302544.4392661/94‑1 (MQ=60)
                                                           TCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCA                                   <  SRR4302544.4120860/94‑1 (MQ=60)
                                                           TCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCA                                   >  SRR4302544.4058659/1‑94 (MQ=60)
                                                           TCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCA                                   <  SRR4302544.2314989/94‑1 (MQ=60)
                                                            CCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCAC                                  >  SRR4302544.3011815/1‑94 (MQ=60)
                                                               CGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGC                               >  SRR4302544.4390346/1‑94 (MQ=60)
                                                               CGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGC                               >  SRR4302544.721503/1‑94 (MQ=60)
                                                                GTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCA                              >  SRR4302544.953383/1‑94 (MQ=60)
                                                                GTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCA                              >  SRR4302544.1089240/1‑94 (MQ=60)
                                                                 TATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAG                             <  SRR4302544.4681481/94‑1 (MQ=60)
                                                                 TATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAG                             >  SRR4302544.2707560/1‑94 (MQ=60)
                                                                 TATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGAATGAAAAAGTCACCAAACAGCAAAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAG                             <  SRR4302544.3696296/94‑1 (MQ=60)
                                                                  ATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGG                            >  SRR4302544.580826/1‑94 (MQ=60)
                                                                  ATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGG                            >  SRR4302544.4442773/1‑94 (MQ=60)
                                                                  ATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGG                            >  SRR4302544.2169462/1‑94 (MQ=60)
                                                                  ATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGG                            <  SRR4302544.2025725/94‑1 (MQ=60)
                                                                   atgggaggtAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGC                           <  SRR4302544.4790783/85‑1 (MQ=60)
                                                                   TTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGC                           <  SRR4302544.3420647/94‑1 (MQ=60)
                                                                    TGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCG                          >  SRR4302544.3827944/1‑94 (MQ=60)
                                                                     GGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGG                         <  SRR4302544.3302973/94‑1 (MQ=60)
                                                                     GGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGG                         >  SRR4302544.17940/1‑94 (MQ=60)
                                                                     GGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGG                         >  SRR4302544.3197511/1‑94 (MQ=60)
                                                                      GCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGA                        <  SRR4302544.2090154/94‑1 (MQ=60)
                                                                       CAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAG                       >  SRR4302544.2537679/1‑94 (MQ=60)
                                                                       CAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAG                       >  SRR4302544.438536/1‑94 (MQ=60)
                                                                       CAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAG                       <  SRR4302544.2878381/94‑1 (MQ=60)
                                                                        AGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGT                      <  SRR4302544.62168/94‑1 (MQ=60)
                                                                        AGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGT                      <  SRR4302544.3600269/94‑1 (MQ=60)
                                                                        AGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGT                      >  SRR4302544.47291/1‑94 (MQ=60)
                                                                         GTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTT                     >  SRR4302544.4172129/1‑94 (MQ=60)
                                                                         GTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTT                     <  SRR4302544.2564232/94‑1 (MQ=60)
                                                                           AAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGG                   <  SRR4302544.3885729/94‑1 (MQ=60)
                                                                              ACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAAT                <  SRR4302544.1267605/94‑1 (MQ=60)
                                                                               CCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATT               <  SRR4302544.1311291/94‑1 (MQ=60)
                                                                               CCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATT               <  SRR4302544.3715774/94‑1 (MQ=60)
                                                                               CCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATT               <  SRR4302544.637045/94‑1 (MQ=60)
                                                                                CCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTA              >  SRR4302544.907085/1‑94 (MQ=60)
                                                                                CCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTA              <  SRR4302544.455420/94‑1 (MQ=60)
                                                                                CCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTA              >  SRR4302544.475147/1‑94 (MQ=60)
                                                                                CCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTA              <  SRR4302544.2654533/94‑1 (MQ=60)
                                                                                CCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTA              <  SRR4302544.2654532/94‑1 (MQ=60)
                                                                                CCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGGTGGAATTA              >  SRR4302544.1019510/1‑94 (MQ=60)
                                                                                 CCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTAC             <  SRR4302544.2701522/94‑1 (MQ=60)
                                                                                 CCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTAC             >  SRR4302544.945818/1‑94 (MQ=60)
                                                                                  CTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACT            <  SRR4302544.2658969/94‑1 (MQ=60)
                                                                                  CTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACT            >  SRR4302544.2859376/1‑94 (MQ=60)
                                                                                   TGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTG           >  SRR4302544.3019118/1‑94 (MQ=60)
                                                                                    GTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGG          <  SRR4302544.3311868/94‑1 (MQ=60)
                                                                                    GTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGG          <  SRR4302544.2342234/94‑1 (MQ=60)
                                                                                    GTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTGTCACCGCAGGCGGAGTTGGAATTACTGG          <  SRR4302544.2342235/94‑1 (MQ=60)
                                                                                     TTCCATTGGGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGGTGGAATTACTGGA         >  SRR4302544.2356964/1‑94 (MQ=60)
                                                                                     TTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGA         <  SRR4302544.2863444/94‑1 (MQ=60)
                                                                                      TCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAA        <  SRR4302544.2210136/94‑1 (MQ=60)
                                                                                      TCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAA        >  SRR4302544.1100092/1‑94 (MQ=60)
                                                                                       CCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAA       >  SRR4302544.4697010/1‑94 (MQ=60)
                                                                                       CCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAA       >  SRR4302544.46602/1‑94 (MQ=60)
                                                                                         ATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACG     <  SRR4302544.1285750/94‑1 (MQ=60)
                                                                                           TGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACGGA   >  SRR4302544.2030553/1‑94 (MQ=60)
                                                                                            GAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACGGAT  <  SRR4302544.2831982/94‑1 (MQ=60)
                                                                                            GAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACGGAT  >  SRR4302544.2775293/1‑94 (MQ=60)
                                                                                            GAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACGGAT  >  SRR4302544.762554/1‑94 (MQ=60)
                                                                                             |                                                                                            
AGCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTATTCCATTGGGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACGGAT  >  NC_000913/4049056‑4049241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑