Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,128,799 T→C V462A (GTA→GCA)  metL → aspartokinase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,128,7990TC98.8% 525.6 / ‑3.2 164V462A (GTA→GCA) metLaspartokinase II
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (82/80);  total (83/81)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.95e-01

Only 100 of 168 total aligned reads displayed.

TGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGT  >  NC_000913/4128709‑4128888
                                                                                          |                                                                                         
tggtggCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCAt                                                                                         <  2:2228588/93‑1 (MQ=255)
tggtggCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCAt                                                                                         <  2:2228589/93‑1 (MQ=255)
tggtggCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCAt                                                                                         <  1:2084481/93‑1 (MQ=255)
tggtggAAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTACGACTTCCGCGCAGAACAAAGCATCGGCCTGGCAt                                                                                         >  2:4591795/1‑93 (MQ=255)
 ggtggCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCAtt                                                                                        <  1:860979/93‑1 (MQ=255)
 ggtggCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCAtt                                                                                        <  2:700980/93‑1 (MQ=255)
 ggtggCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCAtt                                                                                        <  2:3848886/93‑1 (MQ=255)
      cAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCg                                                                                   <  1:1088624/93‑1 (MQ=255)
       aGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCgg                                                                                  <  2:4381303/93‑1 (MQ=255)
       aGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCgg                                                                                  >  1:4614237/1‑93 (MQ=255)
        gTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGt                                                                                 <  2:1024337/93‑1 (MQ=255)
        gTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGt                                                                                 <  2:1024336/93‑1 (MQ=255)
         tACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTa                                                                                >  2:4729572/1‑93 (MQ=255)
          aCTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTaa                                                                               <  1:4611431/93‑1 (MQ=255)
          aCTGCGCACCGGCCAGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTaa                                                                               <  1:692052/93‑1 (MQ=255)
            tGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAgg                                                                             <  2:3604108/93‑1 (MQ=255)
             gcgcACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAggg                                                                            >  2:476076/1‑93 (MQ=255)
             gcgcACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAggg                                                                            <  2:1813280/93‑1 (MQ=255)
             gcgcACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAggg                                                                            <  2:1280742/93‑1 (MQ=255)
               gcACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCa                                                                          >  2:3770289/1‑93 (MQ=255)
               gcACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCa                                                                          >  2:939560/1‑93 (MQ=255)
               gcACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCa                                                                          <  1:4682720/93‑1 (MQ=255)
                 aCCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAat                                                                        >  1:920818/1‑93 (MQ=255)
                  ccGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAata                                                                       <  1:4187717/93‑1 (MQ=255)
                  ccGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAata                                                                       <  1:403929/93‑1 (MQ=255)
                   cGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAatat                                                                      <  1:500322/93‑1 (MQ=255)
                    ggCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCGTCGGCCTGGCATTGTTCGGTAAGGGCAATATc                                                                     <  1:4107708/93‑1 (MQ=255)
                    ggCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATc                                                                     >  2:1556318/1‑93 (MQ=255)
                    agCCCGACCGAAAGCCTGATTCAGGCGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACCTATCGGCCTGGCATTGTTCGGTAAGGGCAATATc                                                                     <  2:1782381/92‑1 (MQ=255)
                         gaccgaAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTc                                                                >  1:4511402/1‑93 (MQ=255)
                         gaccgaAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTc                                                                >  2:1181678/1‑93 (MQ=255)
                          accgaAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTcc                                                               >  1:4083841/1‑93 (MQ=255)
                          accgaAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGATTcc                                                               >  1:4452771/1‑93 (MQ=255)
                            cgaAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGGTCCCg                                                             >  2:2004472/1‑93 (MQ=255)
                             gaAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGt                                                            >  1:3684545/1‑93 (MQ=255)
                             gaAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGt                                                            <  1:3679186/93‑1 (MQ=255)
                             gaAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGt                                                            <  2:348210/93‑1 (MQ=255)
                             gaAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGt                                                            <  1:949362/93‑1 (MQ=255)
                             gaAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGt                                                            <  1:1378277/93‑1 (MQ=255)
                               aaGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGCCAATATCGGTTCCCGCTg                                                          >  1:2117153/1‑93 (MQ=255)
                                aGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTgg                                                         >  1:3762346/1‑93 (MQ=255)
                                 gCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGc                                                        >  1:426679/1‑93 (MQ=255)
                                 gCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGc                                                        >  2:4365646/1‑93 (MQ=255)
                                   cTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTg                                                      <  2:2380199/93‑1 (MQ=255)
                                   cTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTg                                                      >  2:2415610/1‑93 (MQ=255)
                                   cTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTg                                                      <  1:982523/93‑1 (MQ=255)
                                    tGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGCAAGGGCAATATCGGTTCCCGTTGGCTgg                                                     >  1:1002532/1‑93 (MQ=255)
                                      aTTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGaa                                                   >  1:333920/1‑93 (MQ=255)
                                       ttCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATAGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAAc                                                  <  2:2157269/93‑1 (MQ=255)
                                           ggggCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGtt                                              >  1:2051836/1‑93 (MQ=255)
                                            gggCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTc                                             <  2:2047264/93‑1 (MQ=255)
                                              gCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGc                                           <  2:367090/93‑1 (MQ=255)
                                                tGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGccc                                         >  1:2767024/1‑93 (MQ=255)
                                                tGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGccc                                         >  2:4010372/1‑93 (MQ=255)
                                                tGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGccc                                         >  1:2012651/1‑93 (MQ=255)
                                                 gCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCg                                        <  1:3844969/93‑1 (MQ=255)
                                                 gCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCg                                        >  2:2963431/1‑93 (MQ=255)
                                                 gCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCg                                        <  1:3434733/93‑1 (MQ=255)
                                                  cATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGt                                       <  1:2898337/93‑1 (MQ=255)
                                                  cATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGt                                       >  2:1875188/1‑93 (MQ=255)
                                                   aTCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTg                                      >  2:1469506/1‑93 (MQ=255)
                                                   aTCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTg                                      >  2:2321576/1‑93 (MQ=255)
                                                    tCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCACTATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTga                                     >  1:2282487/1‑93 (MQ=255)
                                                    tCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTga                                     >  1:2597902/1‑93 (MQ=255)
                                                    tCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTga                                     >  1:2282486/1‑93 (MQ=255)
                                                       gTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTgagca                                  >  1:2412814/1‑93 (MQ=255)
                                                        tCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTgagcag                                 >  1:279878/1‑93 (MQ=255)
                                                         ccGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTgagcaga                                >  1:4251269/1‑93 (MQ=255)
                                                           gTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTgagcagagc                              >  1:2731811/1‑93 (MQ=255)
                                                            tCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTgagcagagca                             <  1:219438/93‑1 (MQ=255)
                                                            tCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTgagcagagca                             >  2:1279209/1‑93 (MQ=255)
                                                              ttCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACg                           >  2:779951/1‑93 (MQ=255)
                                                              ttCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACg                           >  2:2922498/1‑93 (MQ=255)
                                                               tCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGc                          <  1:2518506/93‑1 (MQ=255)
                                                                 cgcgCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCtt                        <  1:30325/93‑1 (MQ=255)
                                                                  gcgcAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGGACTGTTCGCCCGTGAGCAGAGCACGCttt                       >  1:3820821/1‑93 (MQ=255)
                                                                  gcgcAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCttt                       <  1:656503/93‑1 (MQ=255)
                                                                  gcgcAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCttt                       <  2:3211800/93‑1 (MQ=255)
                                                                  gcgcAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCttt                       <  1:445634/93‑1 (MQ=255)
                                                                  gcgcAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTACCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCttt                       >  2:2584147/1‑93 (MQ=255)
                                                                   cgcAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTc                      <  1:4422925/93‑1 (MQ=255)
                                                                   cgcAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTc                      <  1:1941879/93‑1 (MQ=255)
                                                                     cAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCgg                    >  1:123290/1‑93 (MQ=255)
                                                                      aGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGc                   >  1:251913/1‑93 (MQ=255)
                                                                         aaaaCGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACg                >  2:580779/1‑93 (MQ=255)
                                                                          aaaCGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGt               >  1:2864238/1‑93 (MQ=255)
                                                                            aCGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTAc             >  2:268955/1‑93 (MQ=255)
                                                                             cGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTAcc            <  2:1427820/93‑1 (MQ=255)
                                                                               cATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCgg          <  2:3987865/93‑1 (MQ=255)
                                                                                aTCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGc         <  2:4440755/93‑1 (MQ=255)
                                                                                aTCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGc         >  1:1817829/1‑93 (MQ=255)
                                                                                aTCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGc         >  2:630885/1‑93 (MQ=255)
                                                                                  cGGCCTTGGATTGTTCGGTAAGGGCCATATCCGTTCCCGTTGGCTGGAACTGTTCTCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCtt       <  1:1543977/93‑1 (MQ=255)
                                                                                  cGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCtt       >  2:480559/1‑93 (MQ=255)
                                                                                    gCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTGGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTg     >  1:3197306/1‑93 (MQ=255)
                                                                                    gCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCTGCTTTg     >  2:3458310/1‑93 (MQ=255)
                                                                                    gCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTg     >  2:4642525/1‑93 (MQ=255)
                                                                                      cTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAg   >  2:1391952/1‑93 (MQ=255)
                                                                                      cTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAg   >  2:4315117/1‑93 (MQ=255)
                                                                                       tGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGt  >  1:3373555/1‑93 (MQ=255)
                                                                                          |                                                                                         
TGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGT  >  NC_000913/4128709‑4128888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCCTGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGT  >  NC_000913/4128706‑4128892
                                                                                             |                                                                                             
GCCTGGTGGCAGTACTGCGCACCGGCCCGGCCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGC                                                                                               <  SRR4302544.1279844/94‑1 (MQ=60)
 CCTGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCA                                                                                              >  SRR4302544.3369601/1‑94 (MQ=60)
  CTGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCAT                                                                                             >  SRR4302544.4026631/1‑94 (MQ=60)
   TGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATT                                                                                            <  SRR4302544.2104531/94‑1 (MQ=60)
   TGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATT                                                                                            <  SRR4302544.495746/94‑1 (MQ=60)
   TGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATT                                                                                            <  SRR4302544.184097/94‑1 (MQ=60)
    GGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTG                                                                                           <  SRR4302544.869497/94‑1 (MQ=60)
         CAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGG                                                                                      <  SRR4302544.1099449/94‑1 (MQ=60)
         CAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGG                                                                                      >  SRR4302544.4660045/1‑94 (MQ=60)
             ACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAG                                                                                  <  SRR4302544.4657226/94‑1 (MQ=60)
             ACTGCGCACCGGCCAGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAG                                                                                  <  SRR4302544.698947/94‑1 (MQ=60)
               TGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGG                                                                                <  SRR4302544.4541319/94‑1 (MQ=60)
                 CGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCA                                                                              >  SRR4302544.2083955/1‑94 (MQ=60)
                  GCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAA                                                                             <  SRR4302544.4729009/94‑1 (MQ=60)
                   CACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAAT                                                                            >  SRR4302544.929903/1‑94 (MQ=60)
                   CACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAAT                                                                            <  SRR4302544.1243021/94‑1 (MQ=60)
                     CCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATAT                                                                          <  SRR4302544.408031/94‑1 (MQ=60)
                     CCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATAT                                                                          <  SRR4302544.4229197/94‑1 (MQ=60)
                      CGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATC                                                                         <  SRR4302544.505238/94‑1 (MQ=60)
                       GGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCGTCGGCCTGGCATTGTTCGGTAAGGGCAATATCG                                                                        <  SRR4302544.4148547/94‑1 (MQ=60)
                       GGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCG                                                                        <  SRR4302544.670732/94‑1 (MQ=60)
                       GGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCG                                                                        <  SRR4302544.4189959/94‑1 (MQ=60)
                        GCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGG                                                                       >  SRR4302544.2047913/1‑94 (MQ=60)
                        GCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGG                                                                       <  SRR4302544.4478818/94‑1 (MQ=60)
                        GCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGG                                                                       <  SRR4302544.3788527/94‑1 (MQ=60)
                           CGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTC                                                                    >  SRR4302544.4556615/1‑94 (MQ=60)
                            GACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCC                                                                   >  SRR4302544.4124463/1‑94 (MQ=60)
                            GACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCC                                                                   >  SRR4302544.345324/1‑94 (MQ=60)
                            GACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGATTCC                                                                   >  SRR4302544.4497623/1‑94 (MQ=60)
                            GACCGAAAGCCTGATTCAGGGGCTGCATCAGTCAGTCTTACGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCC                                                                   <  SRR4302544.2583406/94‑1 (MQ=60)
                             ACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCC                                                                  >  SRR4302544.884019/1‑94 (MQ=60)
                              CCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCG                                                                 >  SRR4302544.1669689/1‑94 (MQ=60)
                               CGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGT                                                                >  SRR4302544.3721282/1‑94 (MQ=60)
                                GAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTT                                                               >  SRR4302544.2819146/1‑94 (MQ=60)
                                GAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTT                                                               <  SRR4302544.1392030/94‑1 (MQ=60)
                                GAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTT                                                               <  SRR4302544.958701/94‑1 (MQ=60)
                                GAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTT                                                               <  SRR4302544.3715883/94‑1 (MQ=60)
                                 AAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGCCAATATCGGTTCCCGCTG                                                              >  SRR4302544.2137487/1‑94 (MQ=60)
                                  AAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGG                                                             >  SRR4302544.3799682/1‑94 (MQ=60)
                                   AGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGC                                                            >  SRR4302544.4271147/1‑94 (MQ=60)
                                   AGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGC                                                            >  SRR4302544.431016/1‑94 (MQ=60)
                                   AGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGC                                                            <  SRR4302544.2378704/94‑1 (MQ=60)
                                      CTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGG                                                         <  SRR4302544.992154/94‑1 (MQ=60)
                                      CTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGG                                                         <  SRR4302544.2849456/94‑1 (MQ=60)
                                      CTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGCAAGGGCAATATCGGTTCCCGTTGGCTGG                                                         >  SRR4302544.1012487/1‑94 (MQ=60)
                                      CTGATTCAGGGGCTGCATCAGACCGTGTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGG                                                         <  SRR4302544.992155/94‑1 (MQ=60)
                                        GATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAA                                                       >  SRR4302544.337387/1‑94 (MQ=60)
                                          TTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACT                                                     <  SRR4302544.3244500/94‑1 (MQ=60)
                                           TCAGGGGCTCCATCAGTCCGTCTCCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTG                                                    <  SRR4302544.965073/94‑1 (MQ=60)
                                             AGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTT                                                  >  SRR4302544.2071589/1‑94 (MQ=60)
                                              GGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTC                                                 <  SRR4302544.3549518/94‑1 (MQ=60)
                                               GGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCG                                                <  SRR4302544.4598827/94‑1 (MQ=60)
                                               GGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCG                                                <  SRR4302544.4408901/94‑1 (MQ=60)
                                                GGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGC                                               <  SRR4302544.2180215/94‑1 (MQ=60)
                                                  CTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCC                                             >  SRR4302544.2793633/1‑94 (MQ=60)
                                                  CTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCC                                             >  SRR4302544.2032212/1‑94 (MQ=60)
                                                    GCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGT                                           <  SRR4302544.3883115/94‑1 (MQ=60)
                                                    GCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGT                                           >  SRR4302544.2522829/1‑94 (MQ=60)
                                                    GCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGT                                           <  SRR4302544.3467567/94‑1 (MQ=60)
                                                     CATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTG                                          <  SRR4302544.2926252/94‑1 (MQ=60)
                                                      ATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCACTATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGA                                         >  SRR4302544.2303992/1‑94 (MQ=60)
                                                      ATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGA                                         >  SRR4302544.2303991/1‑94 (MQ=60)
                                                      ATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGA                                         >  SRR4302544.2622877/1‑94 (MQ=60)
                                                         AGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCA                                      >  SRR4302544.2435761/1‑94 (MQ=60)
                                                          GTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAG                                     >  SRR4302544.282803/1‑94 (MQ=60)
                                                           TCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGA                                    >  SRR4302544.4294611/1‑94 (MQ=60)
                                                             CGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGC                                  >  SRR4302544.4696129/1‑94 (MQ=60)
                                                             CGTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGC                                  >  SRR4302544.2758143/1‑94 (MQ=60)
                                                              GTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCA                                 >  SRR4302544.826757/1‑94 (MQ=60)
                                                              GTCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGAAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCA                                 <  SRR4302544.1291032/94‑1 (MQ=60)
                                                               TCTTCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCAC                                <  SRR4302544.221707/94‑1 (MQ=60)
                                                                  TCCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCT                             <  SRR4302544.2542697/94‑1 (MQ=60)
                                                                   CCGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTT                            >  SRR4302544.2705288/1‑94 (MQ=60)
                                                                    CGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGGACTGTTCGCCCGTGAGCAGAGCACGCTTT                           >  SRR4302544.3858696/1‑94 (MQ=60)
                                                                    CGCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTT                           <  SRR4302544.30612/94‑1 (MQ=60)
                                                                     GCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTC                          <  SRR4302544.450082/94‑1 (MQ=60)
                                                                     GCGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTC                          <  SRR4302544.663000/94‑1 (MQ=60)
                                                                      CGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCG                         <  SRR4302544.1960672/94‑1 (MQ=60)
                                                                      CGCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCG                         <  SRR4302544.4467547/94‑1 (MQ=60)
                                                                       GCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGG                        <  SRR4302544.3844294/94‑1 (MQ=60)
                                                                       GCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGG                        >  SRR4302544.3076455/1‑94 (MQ=60)
                                                                       GCAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGG                        >  SRR4302544.124420/1‑94 (MQ=60)
                                                                        CAGAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGC                       >  SRR4302544.254558/1‑94 (MQ=60)
                                                                          GAAAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCAC                     >  SRR4302544.2963470/1‑94 (MQ=60)
                                                                            AAAACGCATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGT                   >  SRR4302544.2891890/1‑94 (MQ=60)
                                                                              AACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTAC                 >  SRR4302544.4080207/1‑94 (MQ=60)
                                                                                  CATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGC             >  SRR4302544.2299030/1‑94 (MQ=60)
                                                                                  CATCGGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGC             >  SRR4302544.1835564/1‑94 (MQ=60)
                                                                                     CGGCCTTGGATTGTTCGGTAAGGGCCATATCCGTTCCCGTTGGCTGGAACTGTTCTCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTT          <  SRR4302544.1559347/94‑1 (MQ=60)
                                                                                      GGCCTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTGGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTG         >  SRR4302544.3227781/1‑94 (MQ=60)
                                                                                         CTGGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGT      >  SRR4302544.3405763/1‑94 (MQ=60)
                                                                                           GGCATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTT    <  SRR4302544.1809167/94‑1 (MQ=60)
                                                                                             CATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGT  <  SRR4302544.2094643/94‑1 (MQ=60)
                                                                                             CATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGT  >  SRR4302544.2013400/1‑94 (MQ=60)
                                                                                             |                                                                                             
GCCTGGTGGCAGTACTGCGCACCGGCCCGACCGAAAGCCTGATTCAGGGGCTGCATCAGTCCGTCTTCCGCGCAGAAAAACGCATCGGCCTGGTATTGTTCGGTAAGGGCAATATCGGTTCCCGTTGGCTGGAACTGTTCGCCCGTGAGCAGAGCACGCTTTCGGCACGTACCGGCTTTGAGTTTGT  >  NC_000913/4128706‑4128892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑