Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,787,068 G→A A204T (GCC→ACC)  aroH → 3‑deoxy‑D‑arabinoheptulosonate‑7‑phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,787,0680GA99.5% 682.9 / NA 197A204T (GCC→ACC) aroH3‑deoxy‑D‑arabinoheptulosonate‑7‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (102/93);  total (102/95)
Fisher's exact test for biased strand distribution p-value = 4.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 225 total aligned reads displayed.

ATGGCTTCGGCACTCTCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCGCCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTATTATGCGT  >  NC_000913/1786978‑1787157
                                                                                          |                                                                                         
aTGGCTTCGGCACTCTCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCacc                                                                                         <  2:6402318/93‑1 (MQ=255)
     ttCGGCACTCTCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCGGCGCAGCCCGCaccagcca                                                                                    <  1:6543459/93‑1 (MQ=255)
     ttCGGCACTCTCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCaccagcca                                                                                    >  2:6002855/1‑93 (MQ=255)
     ttCGGCACTCTCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCaccagcca                                                                                    >  1:1947834/1‑93 (MQ=255)
       cGGCACTCTCCTGTCCGTTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCata                                                                                  <  2:3261694/93‑1 (MQ=255)
       cGGCACTCTCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCata                                                                                  <  2:100560/93‑1 (MQ=255)
       cGGCACTCTCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCata                                                                                  <  2:27627/93‑1 (MQ=255)
       cGGCACTCTCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCata                                                                                  <  2:6049916/93‑1 (MQ=255)
        ggCGCTCTCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCatat                                                                                 <  2:5330256/93‑1 (MQ=255)
         gCACTCTCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATg                                                                                <  1:3179658/93‑1 (MQ=255)
         gCACTCTCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATg                                                                                <  2:2579019/93‑1 (MQ=255)
          cACTCTCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGt                                                                               <  1:3606281/93‑1 (MQ=255)
           aCTCTCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGtt                                                                              >  2:2437068/1‑93 (MQ=255)
           aCTCTCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGtt                                                                              >  1:5832392/1‑93 (MQ=255)
             tctcCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTcc                                                                            <  1:2630955/93‑1 (MQ=255)
             tctcCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTcc                                                                            <  2:3200073/93‑1 (MQ=255)
                ccTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCCCCAGCCATATGTTCctct                                                                         <  1:5059206/93‑1 (MQ=255)
                   gTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGc                                                                      >  2:6307508/1‑93 (MQ=255)
                    tCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGcc                                                                     >  2:3366272/1‑93 (MQ=255)
                    tCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGcc                                                                     >  2:4534023/1‑93 (MQ=255)
                     ccGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCa                                                                    >  1:5283956/1‑93 (MQ=255)
                       ggTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGa                                                                  >  1:6510223/1‑93 (MQ=255)
                        gTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGAc                                                                 >  2:4277427/1‑93 (MQ=255)
                        gTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGAc                                                                 >  2:4672765/1‑93 (MQ=255)
                         tAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACa                                                                <  1:1471069/93‑1 (MQ=255)
                         tAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACa                                                                >  2:2092064/1‑93 (MQ=255)
                          aGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACaa                                                               >  1:4943440/1‑93 (MQ=255)
                          aGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGCTCCTCTCGCCAGACaa                                                               >  1:2599754/1‑93 (MQ=255)
                           ggTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACaaa                                                              <  2:5535824/93‑1 (MQ=255)
                            gTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACaaaa                                                             >  2:3005438/1‑93 (MQ=255)
                            gTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACaaaa                                                             <  2:3910199/93‑1 (MQ=255)
                            gTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACaaaa                                                             >  2:6523933/1‑93 (MQ=255)
                            gTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACaaaa                                                             <  1:4417153/93‑1 (MQ=255)
                            gTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACaaaa                                                             >  2:3466170/1‑93 (MQ=255)
                             ttttAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACaaaaa                                                            <  1:818211/93‑1 (MQ=255)
                             ttttAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACaaaaa                                                            >  2:147671/1‑93 (MQ=255)
                             ttttAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACaaaaa                                                            <  1:2531934/93‑1 (MQ=255)
                              tttAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAAt                                                           <  2:443031/93‑1 (MQ=255)
                               ttAAAAATGGTACCGATGGCAATACTCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTTGCCAGACAAAAATg                                                          >  1:6731142/1‑93 (MQ=255)
                               ttAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATg                                                          >  1:4825861/1‑93 (MQ=255)
                                 aaaaaTGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGt                                                        >  1:2449515/1‑93 (MQ=255)
                                  aaaaTGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTc                                                       >  2:6632061/1‑93 (MQ=255)
                                   aaaTGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATTGTCa                                                      <  2:4029679/93‑1 (MQ=255)
                                   aaaTGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCa                                                      >  2:5830905/1‑93 (MQ=255)
                                   aaaTGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCa                                                      >  2:5098943/1‑93 (MQ=255)
                                   aaaTGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCa                                                      >  1:1519447/1‑93 (MQ=255)
                                     aTGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGa                                                    >  2:1778487/1‑93 (MQ=255)
                                     aTGGTACCGATGGCAATACGCGGATTGCGGTGGATGCTACCCGGGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGa                                                    <  2:4963107/93‑1 (MQ=255)
                                       ggTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATg                                                  >  2:2732926/1‑93 (MQ=255)
                                       ggTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATg                                                  >  1:5707626/1‑93 (MQ=255)
                                        gTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGa                                                 <  2:1336726/93‑1 (MQ=255)
                                            cGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATAGTCAGATGACCAt                                             >  1:1074360/1‑93 (MQ=255)
                                             gATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATc                                            <  1:3525285/93‑1 (MQ=255)
                                              aTGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCCCCAGACAAAAATGGTCAGAGGACCATCt                                           >  1:6381293/1‑93 (MQ=255)
                                               tGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTa                                          >  1:6163838/1‑93 (MQ=255)
                                                 gCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGTTGACCATCTATc                                        <  2:3591421/93‑1 (MQ=255)
                                                   aaTACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAg                                      >  2:841168/1‑93 (MQ=255)
                                                    agaCGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGa                                     >  1:4203593/3‑93 (MQ=255)
                                                    aTACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGa                                     >  1:3065379/1‑93 (MQ=255)
                                                     tACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGAc                                    >  1:935607/1‑93 (MQ=255)
                                                     tACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGAc                                    >  1:1675990/1‑93 (MQ=255)
                                                      aCGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGAcc                                   >  2:2881525/1‑93 (MQ=255)
                                                       cgcgGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCa                                  >  1:3686106/1‑93 (MQ=255)
                                                       cgcgGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCa                                  <  1:2217711/93‑1 (MQ=255)
                                                       cgcgGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCa                                  <  1:4920905/93‑1 (MQ=255)
                                                        gcgGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAg                                 <  2:6218866/93‑1 (MQ=255)
                                                        ccgGATTGCTGTGGATGCTATCCGCCCAGCCCGCCCCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAg                                 <  1:5592615/92‑1 (MQ=255)
                                                         cgGATTGCTGTGGATGCTACCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGACGACCATCTATCAGACCAGc                                <  1:3278968/93‑1 (MQ=255)
                                                          ggATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCg                               <  2:3121578/93‑1 (MQ=255)
                                                            aTTGCTGTGGATCCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGc                             <  1:6267038/93‑1 (MQ=255)
                                                                cTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAAcc                         <  1:5441752/93‑1 (MQ=255)
                                                                 tgtgGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAAccc                        >  2:4742832/1‑93 (MQ=255)
                                                                 tgtgGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAAccc                        >  2:1138268/1‑93 (MQ=255)
                                                                     gATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTAt                    <  2:6046027/93‑1 (MQ=255)
                                                                     gATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTAt                    <  1:1135118/93‑1 (MQ=255)
                                                                      aTGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATg                   <  2:3980657/93‑1 (MQ=255)
                                                                        gCTACCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGc                 <  2:861644/93‑1 (MQ=255)
                                                                         cTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGcc                <  1:202725/93‑1 (MQ=255)
                                                                           acccGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCcac              <  1:3438300/91‑1 (MQ=255)
                                                                            tCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCcaca             >  1:467873/1‑93 (MQ=255)
                                                                             ccGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACat            <  2:407645/93‑1 (MQ=255)
                                                                             ccGCGCACCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACat            <  2:2833034/93‑1 (MQ=255)
                                                                               gcgcAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACatta          >  1:6637132/1‑93 (MQ=255)
                                                                               gcgcAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACatta          >  2:6267018/1‑93 (MQ=255)
                                                                               gcgcAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACatta          >  1:2869219/1‑93 (MQ=255)
                                                                                cgcAGCCCTCACCAGCCATATTTTCCTTTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGGAACCAGTATGGCCACattat         <  1:116273/93‑1 (MQ=255)
                                                                                cgcAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACattat         >  2:230900/1‑93 (MQ=255)
                                                                                cgcAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACattat         <  2:5013054/93‑1 (MQ=255)
                                                                                 gcAGCCCGCGCCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACattatt        <  1:1236539/93‑1 (MQ=255)
                                                                                 ccAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACattatt        <  2:2489488/92‑1 (MQ=255)
                                                                                  cAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACAttatta       <  2:4572921/93‑1 (MQ=255)
                                                                                  cAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACAttatta       <  1:3421925/93‑1 (MQ=255)
                                                                                   aGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATtattat      >  2:4883545/1‑93 (MQ=255)
                                                                                   aGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATtattat      <  1:5372023/93‑1 (MQ=255)
                                                                                   aGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATtattat      >  2:1933549/1‑93 (MQ=255)
                                                                                     cccGCGCCAGTCATAAGTTCCTCTCGCCAGCCAAAAATGGTCAGATGACCATCTATAAGACCAGCGGAAACCAGTATGGCCACATTATTATGc    <  2:2612694/93‑1 (MQ=255)
                                                                                     cccGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTATTATGc    >  1:4290907/1‑93 (MQ=255)
                                                                                      ccGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTATTATGCg   >  1:2101253/1‑93 (MQ=255)
                                                                                      ccGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTATTATGCg   <  1:1355497/93‑1 (MQ=255)
                                                                                      ccGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTATTATGCg   <  2:3792003/93‑1 (MQ=255)
                                                                                          |                                                                                         
ATGGCTTCGGCACTCTCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCGCCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTATTATGCGT  >  NC_000913/1786978‑1787157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAAATGGCTTCGGCACTCTCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCGCCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTATTATGCGT  >  NC_000913/1786975‑1787157
                                                                                             |                                                                                         
GAAATGGCTTCGGCACTCTCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGGTATCCGCGCAGCCCGCC                                                                                           >  SRR4302536.3638342/1‑94 (MQ=60)
GAAATGGCTTCGGCACTCTCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCA                                                                                           <  SRR4302536.3143825/94‑1 (MQ=60)
 AAATGGCTTCGGCACTCTCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCAC                                                                                          >  SRR4302536.105533/1‑94 (MQ=60)
   ATGGTTTCGACACTCTCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATCCGCGGATTGCTGTGGATGCTATCGGCGCAGCCCGCACCA                                                                                        <  SRR4302536.813386/94‑1 (MQ=60)
      GCTTCGGCACTCTCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCC                                                                                     <  SRR4302536.2623812/94‑1 (MQ=60)
      GCTTCGGCACTCTCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCC                                                                                     <  SRR4302536.1335922/94‑1 (MQ=60)
       CTTCGGCACTCTCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCA                                                                                    <  SRR4302536.966849/94‑1 (MQ=60)
       CTTCGGCACTCTCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCA                                                                                    >  SRR4302536.5713139/1‑94 (MQ=60)
       CTTCGGCACTCTCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCA                                                                                    <  SRR4302536.3477162/94‑1 (MQ=60)
       CTTCGGCACTCTCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCA                                                                                    >  SRR4302536.2006412/1‑94 (MQ=60)
        TTCGGCACTCTCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCGGCGCAGCCCGCACCAGCCAT                                                                                   <  SRR4302536.6738322/94‑1 (MQ=60)
         TCGGCACTCTCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATA                                                                                  <  SRR4302536.1428006/94‑1 (MQ=60)
          CGGCACTCTCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATAT                                                                                 <  SRR4302536.5419141/94‑1 (MQ=60)
            GCACTCTCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGT                                                                               <  SRR4302536.3272886/94‑1 (MQ=60)
             CACTCTCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTT                                                                              >  SRR4302536.6006164/1‑94 (MQ=60)
             CACTCTCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTT                                                                              >  SRR4302536.5199098/1‑94 (MQ=60)
             CACTCTCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTT                                                                              >  SRR4302536.1610083/1‑94 (MQ=60)
             CACTCTCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTT                                                                              <  SRR4302536.3714118/94‑1 (MQ=60)
                TCTCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCT                                                                           <  SRR4302536.2709839/94‑1 (MQ=60)
                TCTCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCT                                                                           >  SRR4302536.13734/1‑94 (MQ=60)
                TCTCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCT                                                                           <  SRR4302536.6450563/94‑1 (MQ=60)
                TATCCAGTCCGGTAGGTTTTAAAAATGGTACTGATGGCAATCCGGGGATTGCTGTGGATGCTATCCGCGCAGCCCGACCCAGGCATATGTTCCT                                                                           <  SRR4302536.3853800/94‑1 (MQ=60)
                 CTCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTC                                                                          >  SRR4302536.2279737/1‑94 (MQ=60)
                  TCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCT                                                                         >  SRR4302536.4452745/1‑94 (MQ=60)
                   CCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCCCCAGCCATATGTTCCTCTC                                                                        <  SRR4302536.5210751/94‑1 (MQ=60)
                    CTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCG                                                                       <  SRR4302536.2496652/94‑1 (MQ=60)
                     TGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGC                                                                      <  SRR4302536.5461547/94‑1 (MQ=60)
                       TCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCA                                                                    >  SRR4302536.3645383/1‑94 (MQ=60)
                       TCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCA                                                                    >  SRR4302536.5441470/1‑94 (MQ=60)
                        CCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAG                                                                   <  SRR4302536.711658/94‑1 (MQ=60)
                         CGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGA                                                                  >  SRR4302536.6704224/1‑94 (MQ=60)
                         CGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGA                                                                  >  SRR4302536.6365574/1‑94 (MQ=60)
                            TAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAA                                                               >  SRR4302536.5091994/1‑94 (MQ=60)
                            TAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAA                                                               >  SRR4302536.5765162/1‑94 (MQ=60)
                            TAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAA                                                               <  SRR4302536.1514587/94‑1 (MQ=60)
                            TAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGCTCCTCTCGCCAGACAA                                                               >  SRR4302536.2677826/1‑94 (MQ=60)
                             AGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAA                                                              >  SRR4302536.5893158/1‑94 (MQ=60)
                              GGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAA                                                             >  SRR4302536.4554858/1‑94 (MQ=60)
                               GTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAA                                                            <  SRR4302536.4549377/94‑1 (MQ=60)
                               GTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAA                                                            >  SRR4302536.3298989/1‑94 (MQ=60)
                                TTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAAT                                                           <  SRR4302536.2608234/94‑1 (MQ=60)
                                TTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAAT                                                           <  SRR4302536.842286/94‑1 (MQ=60)
                                 TTTAAAAATGGTACCGATGGCAATACTCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTTGCCAGACAAAAATG                                                          >  SRR4302536.6931246/1‑94 (MQ=60)
                                 TTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATG                                                          >  SRR4302536.1992704/1‑94 (MQ=60)
                                 TTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATG                                                          >  SRR4302536.4971099/1‑94 (MQ=60)
                                 TTTAAAAATGGTACCGATGGCAATACGCGCATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATG                                                          <  SRR4302536.6796181/94‑1 (MQ=60)
                                   TAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGT                                                        >  SRR4302536.2523510/1‑94 (MQ=60)
                                     AAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCA                                                      <  SRR4302536.6649075/94‑1 (MQ=60)
                                     AAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCA                                                      >  SRR4302536.1564172/1‑94 (MQ=60)
                                      AAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAG                                                     >  SRR4302536.4412420/1‑94 (MQ=60)
                                         TGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATG                                                  >  SRR4302536.5877363/1‑94 (MQ=60)
                                         TGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATG                                                  >  SRR4302536.4891/1‑94 (MQ=60)
                                         TGATACCGATGGGAATCCGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATG                                                  <  SRR4302536.3249565/94‑1 (MQ=60)
                                              CCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCAT                                             <  SRR4302536.2525922/94‑1 (MQ=60)
                                              CCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATAGTCAGATGACCAT                                             >  SRR4302536.1106522/1‑94 (MQ=60)
                                                GATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCT                                           <  SRR4302536.1178015/94‑1 (MQ=60)
                                                GATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCT                                           <  SRR4302536.3630612/94‑1 (MQ=60)
                                                GATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCT                                           <  SRR4302536.4068472/94‑1 (MQ=60)
                                                GATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCT                                           >  SRR4302536.6762586/1‑94 (MQ=60)
                                                GATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCCCCAGACAAAAATGGTCAGAGGACCATCT                                           >  SRR4302536.6571556/1‑94 (MQ=60)
                                                 ATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTA                                          >  SRR4302536.6347444/1‑94 (MQ=60)
                                                   GGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATC                                        <  SRR4302536.1146563/94‑1 (MQ=60)
                                                    GCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCA                                       <  SRR4302536.1240460/94‑1 (MQ=60)
                                                      AATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGA                                     >  SRR4302536.999454/1‑94 (MQ=60)
                                                      AATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGA                                     >  SRR4302536.3155259/1‑94 (MQ=60)
                                                      AAGACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGA                                     >  SRR4302536.4328545/1‑94 (MQ=60)
                                                       ATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGAC                                    >  SRR4302536.963185/1‑94 (MQ=60)
                                                       ATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGAC                                    >  SRR4302536.1724586/1‑94 (MQ=60)
                                                        TACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACC                                   <  SRR4302536.2712652/94‑1 (MQ=60)
                                                         ACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCA                                  >  SRR4302536.3796344/1‑94 (MQ=60)
                                                         ACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCA                                  <  SRR4302536.2044233/94‑1 (MQ=60)
                                                          CGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAG                                 <  SRR4302536.2284777/94‑1 (MQ=60)
                                                          CGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAG                                 <  SRR4302536.5068785/94‑1 (MQ=60)
                                                          CGCGGATGGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAG                                 <  SRR4302536.5070791/94‑1 (MQ=60)
                                                           CCGGATTGCTGTGGATGCTATCCGCCCAGCCCGCCCCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGC                                <  SRR4302536.5758907/94‑1 (MQ=60)
                                                            CGGATTGCTGTGGATGCTACCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGACGACCATCTATCAGACCAGCG                               <  SRR4302536.3375360/94‑1 (MQ=60)
                                                               ATTGCTGTGGATGCTATGCGCCCAGCCCTCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGTTGACCATCTATCAGACCAGCGGCA                            <  SRR4302536.5533619/94‑1 (MQ=60)
                                                               ATTGCTGTGGATCCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCA                            <  SRR4302536.6453750/94‑1 (MQ=60)
                                                                 TGCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAAC                          <  SRR4302536.941/94‑1 (MQ=60)
                                                                  GCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACC                         <  SRR4302536.6541112/94‑1 (MQ=60)
                                                                  GCTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACC                         >  SRR4302536.2766892/1‑94 (MQ=60)
                                                                   CTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCC                        <  SRR4302536.671253/94‑1 (MQ=60)
                                                                   CTGTGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCC                        <  SRR4302536.5604516/94‑1 (MQ=60)
                                                                      TGGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTA                     >  SRR4302536.4734123/1‑94 (MQ=60)
                                                                       GGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAGAATGGTCAGATGACCATCTATCAGACCAGCGGCAAACCGTAT                    >  SRR4302536.6900819/1‑94 (MQ=60)
                                                                       GGATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTAT                    <  SRR4302536.3540240/94‑1 (MQ=60)
                                                                        GATGCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATG                   <  SRR4302536.1169251/94‑1 (MQ=60)
                                                                         ATGCTATCCCCGCAGCCGTCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGG                  <  SRR4302536.3780641/94‑1 (MQ=60)
                                                                           GCTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCC                <  SRR4302536.2694422/94‑1 (MQ=60)
                                                                            CTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCA               >  SRR4302536.2998593/1‑94 (MQ=60)
                                                                            CTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCA               >  SRR4302536.3772576/1‑94 (MQ=60)
                                                                            CTATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCA               <  SRR4302536.208062/94‑1 (MQ=60)
                                                                              ATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACA             >  SRR4302536.480095/1‑94 (MQ=60)
                                                                              ATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACA             <  SRR4302536.1538902/94‑1 (MQ=60)
                                                                              ATCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACA             >  SRR4302536.5122001/1‑94 (MQ=60)
                                                                              ACCCGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACT             <  SRR4302536.3540687/94‑1 (MQ=60)
                                                                                 CGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTA          >  SRR4302536.2953955/1‑94 (MQ=60)
                                                                                 CGCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTA          >  SRR4302536.6834513/1‑94 (MQ=60)
                                                                                  cgGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTAT         <  SRR4302536.3578284/92‑1 (MQ=60)
                                                                                  GCGCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTAT         >  SRR4302536.3442832/1‑94 (MQ=60)
                                                                                   CGCAGCCCTCACCAGCCATATTTTCCTTTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGGAACCAGTATGGCCACATTATT        <  SRR4302536.119347/94‑1 (MQ=60)
                                                                                    GCAGCCCGCGCCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTATTA       <  SRR4302536.1273675/94‑1 (MQ=60)
                                                                                    GCAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTATTA       >  SRR4302536.1789895/1‑94 (MQ=60)
                                                                                     CAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTATTAT      <  SRR4302536.3523770/94‑1 (MQ=60)
                                                                                     CAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTATTAT      <  SRR4302536.5009536/94‑1 (MQ=60)
                                                                                     CAGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTATTAT      >  SRR4302536.5620884/1‑94 (MQ=60)
                                                                                      AGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTATTATG     >  SRR4302536.2106117/1‑94 (MQ=60)
                                                                                      AGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTATTATG     <  SRR4302536.5532279/94‑1 (MQ=60)
                                                                                      AGCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTATTATG     >  SRR4302536.6133861/1‑94 (MQ=60)
                                                                                       GCCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTATTATGC    >  SRR4302536.4419150/1‑94 (MQ=60)
                                                                                        CCCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTATTATGCG   >  SRR4302536.2164437/1‑94 (MQ=60)
                                                                                         CCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTATTATGCGT  <  SRR4302536.2441504/94‑1 (MQ=60)
                                                                                         CCGCACCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTATTATGCGT  <  SRR4302536.1395895/94‑1 (MQ=60)
                                                                                             |                                                                                         
GAAATGGCTTCGGCACTCTCCTGTCCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGCGCAGCCCGCGCCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTATCAGACCAGCGGCAACCCGTATGGCCACATTATTATGCGT  >  NC_000913/1786975‑1787157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑