Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1475	266.7	13469680	98.2%	13227225	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,953,902	T→C	Q377R (CAG→CGG)	b1872 ←	biotin sulfoxide reductase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,953,902	0	T	C	99.5%	665.9 / ‑5.7	205	Q377R (CAG→CGG)	b1872	biotin sulfoxide reductase 2
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (112/92); total (112/93)
Fisher's exact test for biased strand distribution p-value = 4.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 210 total aligned reads displayed.

ATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATT  >  NC_000913/1953812‑1953991

aTTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGc                                                                                         >  1:1754818/1‑93 (MQ=255)

aTTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGc                                                                                         >  1:2611047/1‑93 (MQ=255)

 tttCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGcc                                                                                        >  1:6316671/1‑93 (MQ=255)

  ttCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGccc                                                                                       <  2:2351171/93‑1 (MQ=255)

  ttCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGccc                                                                                       <  1:4454726/93‑1 (MQ=255)

   tCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCt                                                                                      <  1:1675677/93‑1 (MQ=255)

    ccGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTa                                                                                     <  2:3742429/93‑1 (MQ=255)

     cGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTaa                                                                                    <  2:2969291/93‑1 (MQ=255)

       gcagcaCGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACa                                                                                  >  1:2961024/1‑93 (MQ=255)

       gcagcaCGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACa                                                                                  >  1:2758439/1‑93 (MQ=255)

        cagcaCGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACAt                                                                                 <  2:3635665/93‑1 (MQ=255)

         agcaCGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATc                                                                                >  1:704016/1‑93 (MQ=255)

         agcaCGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATc                                                                                >  2:5643667/1‑93 (MQ=255)

          gcaCGCCGCCAACACGCGTCGGGTTGCGACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCCAACATcc                                                                               >  1:4617864/1‑92 (MQ=255)

          gcaCGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATcg                                                                               >  2:6694891/1‑93 (MQ=255)

          gcaCGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATcg                                                                               >  2:4102368/1‑93 (MQ=255)

               ccgccAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCgccgcc                                                                          >  2:3715230/1‑93 (MQ=255)

               ccgccAACACGCGTCGGGTTACCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCgccgcc                                                                          <  1:456084/93‑1 (MQ=255)

                   cAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCg                                                                      >  2:6402101/1‑93 (MQ=255)

                     acacGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTc                                                                    >  2:2192968/1‑93 (MQ=255)

                     acacGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTc                                                                    <  2:2694745/93‑1 (MQ=255)

                      cacGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCa                                                                   >  1:2806094/1‑93 (MQ=255)

                       acGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCAc                                                                  >  1:505114/1‑93 (MQ=255)

                       acGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCAc                                                                  <  1:5675677/93‑1 (MQ=255)

                         gcgTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCa                                                                <  2:2256377/93‑1 (MQ=255)

                         gcgTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCa                                                                >  2:1921967/1‑93 (MQ=255)

                             cGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCAt                                                            >  2:1237762/1‑93 (MQ=255)

                              gggTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATc                                                           >  2:1780934/1‑93 (MQ=255)

                                gTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCa                                                         >  2:3676112/1‑93 (MQ=255)

                                gTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCa                                                         <  1:5143409/93‑1 (MQ=255)

                                  tGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAgt                                                       <  2:281676/93‑1 (MQ=255)

                                   gCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAgtg                                                      >  2:5950451/1‑93 (MQ=255)

                                   gCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAgtg                                                      >  2:5559027/1‑93 (MQ=255)

                                    ccACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAgtgt                                                     <  2:4464819/93‑1 (MQ=255)

                                    ccACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAgtgt                                                     >  2:6453339/1‑93 (MQ=255)

                                     cACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGtt                                                    >  1:3922138/1‑93 (MQ=255)

                                     cACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGtt                                                    <  1:2225434/93‑1 (MQ=255)

                                         gTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTg                                                >  2:520090/1‑93 (MQ=255)

                                         gTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTg                                                <  2:2520034/93‑1 (MQ=255)

                                         gTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTg                                                <  2:4539520/93‑1 (MQ=255)

                                           tGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGtt                                              >  2:6277524/1‑93 (MQ=255)

                                           tGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGtt                                              <  1:2107999/93‑1 (MQ=255)

                                               gTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCAcc                                          <  2:612846/93‑1 (MQ=255)

                                               gTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCAcc                                          >  2:504398/1‑93 (MQ=255)

                                               gTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCAcc                                          <  1:1410942/93‑1 (MQ=255)

                                               gTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCAcc                                          <  1:3011140/93‑1 (MQ=255)

                                                tAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCa                                         <  2:3428956/93‑1 (MQ=255)

                                                tAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCa                                         >  1:5336585/1‑93 (MQ=255)

                                                tAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCa                                         >  2:5086892/1‑93 (MQ=255)

                                                 aaTGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCat                                        >  1:5114359/1‑93 (MQ=255)

                                                  aTGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCata                                       >  1:5224231/1‑93 (MQ=255)

                                                   tGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCatat                                      >  2:5301466/1‑93 (MQ=255)

                                                   tGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCatat                                      <  2:1499920/93‑1 (MQ=255)

                                                   tGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCatat                                      >  1:5427092/1‑93 (MQ=255)

                                                    gATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATAtt                                     <  2:76377/93‑1 (MQ=255)

                                                    gATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATAtt                                     <  1:3706194/93‑1 (MQ=255)

                                                    gATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATAtt                                     <  1:978414/93‑1 (MQ=255)

                                                    gATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATAtt                                     <  2:1441160/93‑1 (MQ=255)

                                                     aTAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTg                                    >  2:3926445/1‑93 (MQ=255)

                                                      tAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGc                                   >  2:3536509/1‑93 (MQ=255)

                                                        agagAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTACCATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTg                                 <  1:3261712/93‑1 (MQ=255)

                                                        agagAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTg                                 <  2:6219884/93‑1 (MQ=255)

                                                        agagAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTg                                 <  1:2388088/93‑1 (MQ=255)

                                                         gagaAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTgg                                >  1:1630658/1‑93 (MQ=255)

                                                         gagaAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTgg                                >  1:3377587/1‑93 (MQ=255)

                                                         gagaAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTgg                                <  1:858157/93‑1 (MQ=255)

                                                         gagaAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTgg                                >  1:5153881/1‑93 (MQ=255)

                                                            aaaCCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGgcgc                             >  1:1971863/1‑93 (MQ=255)

                                                            aaaCCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGgcgc                             <  2:5683294/93‑1 (MQ=255)

                                                                cAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGaa                         >  2:724572/1‑93 (MQ=255)

                                                                cAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGaa                         <  2:1624635/93‑1 (MQ=255)

                                                                  aaaCCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGACtt                       >  1:1001082/1‑93 (MQ=255)

                                                                  aaaCCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAAtt                       >  1:2196526/1‑93 (MQ=255)

                                                                  aaaCCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAAtt                       >  1:2359625/1‑93 (MQ=255)

                                                                  aaaCCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAAtt                       >  1:6153444/1‑93 (MQ=255)

                                                                    aCCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAAtttc                     >  1:1790549/1‑91 (MQ=255)

                                                                    aCCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTcc                     <  1:2713610/93‑1 (MQ=255)

                                                                     ccgccgCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTccc                    >  1:4351543/1‑93 (MQ=255)

                                                                     ccgccgCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTccc                    <  2:2898162/93‑1 (MQ=255)

                                                                      cgccgcCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTcccc                   <  2:4627517/93‑1 (MQ=255)

                                                                      cgccgcCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTcccc                   <  2:6338356/93‑1 (MQ=255)

                                                                      cgccgcCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTcccc                   >  1:891410/1‑93 (MQ=255)

                                                                       gccgccCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCa                  >  1:5931248/1‑93 (MQ=255)

                                                                       gccgccCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGGGTTTCTGTTCACCATATTGTTGGCGCTGAATTCCCCa                  >  2:3262415/1‑93 (MQ=255)

                                                                            ccGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTg             >  2:3301599/1‑93 (MQ=255)

                                                                             cGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTGACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGc            >  2:4897425/1‑93 (MQ=255)

                                                                             cGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGc            >  1:382371/1‑93 (MQ=255)

                                                                              ggTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGcc           <  1:3358193/93‑1 (MQ=255)

                                                                               gtgtACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCa          >  1:654926/1‑93 (MQ=255)

                                                                                tgtACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCAt         >  1:1420499/1‑93 (MQ=255)

                                                                                tgtACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCAt         >  2:959634/1‑93 (MQ=255)

                                                                                 gtACCAATCCGCCCTAACATCGCCGCCAGCGTCCCCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATc        >  1:4702053/1‑93 (MQ=255)

                                                                                  tACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCa       <  2:1272036/93‑1 (MQ=255)

                                                                                  tACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCa       <  1:3733623/93‑1 (MQ=255)

                                                                                    ccAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGc     <  1:4670070/93‑1 (MQ=255)

                                                                                      aaTCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCAt   <  1:191449/93‑1 (MQ=255)

                                                                                      aaTCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCAt   <  2:4565851/93‑1 (MQ=255)

                                                                                      aaTCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATT‑CCCAGCCTGCCATCAGCAtt  <  2:1097213/93‑1 (MQ=255)

                                                                                       aTCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCAtt  <  2:4704852/93‑1 (MQ=255)

                                                                                       aTCCGCCCTAAAGTTGCCGGCAGCGTCTTCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCCCTGAATTCCCCAGCCTGCCATCAGCAtt  <  2:5315329/93‑1 (MQ=21)

ATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATT  >  NC_000913/1953812‑1953991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTG  >  NC_000913/1953809‑1953994

GACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCC                                                                                              >  SRR4302536.2997975/1‑94 (MQ=60)

  CATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGC                                                                                            >  SRR4302536.2689390/1‑94 (MQ=60)

  CATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGC                                                                                            >  SRR4302536.1806923/1‑94 (MQ=60)

   ATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCC                                                                                           >  SRR4302536.6504829/1‑94 (MQ=60)

     TTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCT                                                                                         <  SRR4302536.4588553/94‑1 (MQ=60)

      TCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTA                                                                                        <  SRR4302536.1724268/94‑1 (MQ=60)

         GGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACA                                                                                     >  SRR4302536.5068913/1‑94 (MQ=60)

         GGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACA                                                                                     >  SRR4302536.3048249/1‑94 (MQ=60)

         GGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACA                                                                                     <  SRR4302536.4035192/94‑1 (MQ=60)

         GGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACA                                                                                     >  SRR4302536.2840521/1‑94 (MQ=60)

           CAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATC                                                                                   >  SRR4302536.724577/1‑94 (MQ=60)

            AGCACGCCGCCAACACGCGTCGGGTTGCGACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCCAACATCC                                                                                  >  SRR4302536.4756580/1‑94 (MQ=60)

            AGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCG                                                                                  <  SRR4302536.1819865/94‑1 (MQ=60)

              CACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCC                                                                                >  SRR4302536.4821009/1‑94 (MQ=60)

              CACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCC                                                                                <  SRR4302536.2906104/94‑1 (MQ=60)

              CACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCC                                                                                >  SRR4302536.3881815/1‑94 (MQ=60)

                  CCGCCAACACGCGTCGGGTTACCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCA                                                                            <  SRR4302536.468041/94‑1 (MQ=60)

                    GCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGC                                                                          >  SRR4302536.6020114/1‑94 (MQ=60)

                     CCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCG                                                                         >  SRR4302536.986559/1‑94 (MQ=60)

                       AACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTC                                                                       >  SRR4302536.204712/1‑94 (MQ=60)

                        ACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCA                                                                      >  SRR4302536.2889304/1‑94 (MQ=60)

                        ACACGCGTCGGGTTGCCACCGTGGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCA                                                                      <  SRR4302536.13599/94‑1 (MQ=60)

                         CACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCAC                                                                     >  SRR4302536.518084/1‑94 (MQ=60)

                          ACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACC                                                                    <  SRR4302536.5844418/94‑1 (MQ=60)

                           CGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCA                                                                   >  SRR4302536.3257927/1‑94 (MQ=60)

                            GCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAG                                                                  <  SRR4302536.1708468/94‑1 (MQ=60)

                             CGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGC                                                                 >  SRR4302536.2744266/1‑94 (MQ=60)

                             CGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGC                                                                 >  SRR4302536.356051/1‑94 (MQ=60)

                              GTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCA                                                                >  SRR4302536.3444281/1‑94 (MQ=60)

                               TCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCAT                                                               <  SRR4302536.1780232/94‑1 (MQ=60)

                                 GGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCC                                                             >  SRR4302536.382080/1‑94 (MQ=60)

                                   GTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAG                                                           <  SRR4302536.5296925/94‑1 (MQ=60)

                                   GTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAG                                                           >  SRR4302536.2263967/1‑94 (MQ=60)

                                       CCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTT                                                       >  SRR4302536.4038694/1‑94 (MQ=60)

                                        CACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTT                                                      <  SRR4302536.2292751/94‑1 (MQ=60)

                                        CACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTT                                                      >  SRR4302536.2253889/1‑94 (MQ=60)

                                        CACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGAATCCAGTGTTT                                                      >  SRR4302536.5276106/1‑94 (MQ=60)

                                        CACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCAAGCGTCACCAGCATCCAGTGTTT                                                      >  SRR4302536.3056882/1‑94 (MQ=60)

                                         ACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTC                                                     >  SRR4302536.742406/1‑94 (MQ=60)

                                          CCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCT                                                    <  SRR4302536.5374710/94‑1 (MQ=60)

                                          CCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCT                                                    <  SRR4302536.54899/94‑1 (MQ=60)

                                              TGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTC                                                <  SRR4302536.2171336/94‑1 (MQ=60)

                                                GAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCAC                                              >  SRR4302536.2346483/1‑94 (MQ=60)

                                                 AGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACC                                             <  SRR4302536.5728775/94‑1 (MQ=60)

                                                 AGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACC                                             >  SRR4302536.1599705/1‑94 (MQ=60)

                                                 AGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACC                                             >  SRR4302536.1453887/1‑94 (MQ=60)

                                                  GTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCA                                            <  SRR4302536.3099651/94‑1 (MQ=60)

                                                  GTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCA                                            <  SRR4302536.1452905/94‑1 (MQ=60)

                                                  GTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCA                                            >  SRR4302536.5496034/1‑94 (MQ=60)

                                                   TAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCAT                                           >  SRR4302536.5267198/1‑94 (MQ=60)

                                                    AATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATA                                          >  SRR4302536.2870606/1‑94 (MQ=60)

                                                    AATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATA                                          >  SRR4302536.5379900/1‑94 (MQ=60)

                                                     ATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATAT                                         >  SRR4302536.5589349/1‑94 (MQ=60)

                                                       GATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTG                                       <  SRR4302536.6544477/94‑1 (MQ=60)

                                                       GATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTG                                       <  SRR4302536.3817000/94‑1 (MQ=60)

                                                       GATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTG                                       <  SRR4302536.1007285/94‑1 (MQ=60)

                                                         TAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCT                                     <  SRR4302536.6802563/94‑1 (MQ=60)

                                                           AGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTACCATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGG                                   <  SRR4302536.3357377/94‑1 (MQ=60)

                                                           AGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGG                                   >  SRR4302536.3477956/1‑94 (MQ=60)

                                                           AGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGG                                   >  SRR4302536.1678125/1‑94 (MQ=60)

                                                           AGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGG                                   <  SRR4302536.2460395/94‑1 (MQ=60)

                                                           AGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGG                                   >  SRR4302536.5307647/1‑94 (MQ=60)

                                                            GAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGC                                  <  SRR4302536.913298/94‑1 (MQ=60)

                                                            GAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGC                                  <  SRR4302536.883379/94‑1 (MQ=60)

                                                              GAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGC                                >  SRR4302536.2031156/1‑94 (MQ=60)

                                                              GAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGC                                <  SRR4302536.5298530/94‑1 (MQ=60)

                                                                AACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTG                              <  SRR4302536.4639467/94‑1 (MQ=60)

                                                                AACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTG                              <  SRR4302536.3312039/94‑1 (MQ=60)

                                                                   CAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAAT                           <  SRR4302536.2814281/94‑1 (MQ=60)

                                                                    AAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGACTT                          >  SRR4302536.1030618/1‑94 (MQ=60)

                                                                    AAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGACTT                          >  SRR4302536.5257740/1‑94 (MQ=60)

                                                                    AAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATT                          >  SRR4302536.2262830/1‑94 (MQ=60)

                                                                    AAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATT                          >  SRR4302536.2431148/1‑94 (MQ=60)

                                                                    AAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATT                          >  SRR4302536.6336768/1‑94 (MQ=60)

                                                                     AAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTC                         >  SRR4302536.5634740/1‑94 (MQ=60)

                                                                     AAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTC                         <  SRR4302536.5415219/94‑1 (MQ=60)

                                                                      AACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTTC                        >  SRR4302536.1844631/1‑94 (MQ=60)

                                                                       ACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCC                       >  SRR4302536.1946045/1‑94 (MQ=60)

                                                                       ACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCC                       >  SRR4302536.2591993/1‑94 (MQ=60)

                                                                       ACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCC                       <  SRR4302536.2794552/94‑1 (MQ=60)

                                                                       ACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCC                       >  SRR4302536.4481734/1‑94 (MQ=60)

                                                                        CCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCC                      >  SRR4302536.917665/1‑94 (MQ=60)

                                                                         CGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCA                     <  SRR4302536.4751551/94‑1 (MQ=60)

                                                                         CGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCA                     <  SRR4302536.2188480/94‑1 (MQ=60)

                                                                         CGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCA                     >  SRR4302536.6107921/1‑94 (MQ=60)

                                                                          GCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAG                    >  SRR4302536.3468988/1‑94 (MQ=60)

                                                                          GCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAG                    <  SRR4302536.6238749/94‑1 (MQ=60)

                                                                            CGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCC                  >  SRR4302536.4791254/1‑94 (MQ=60)

                                                                              CCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTG                >  SRR4302536.4743250/1‑94 (MQ=60)

                                                                               CCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGC               >  SRR4302536.392448/1‑94 (MQ=60)

                                                                                 GGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCA             >  SRR4302536.674124/1‑94 (MQ=60)

                                                                                 GGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCA             >  SRR4302536.2008766/1‑94 (MQ=60)

                                                                                 GGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCA             <  SRR4302536.3457860/94‑1 (MQ=60)

                                                                                  GTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCAT            >  SRR4302536.1462723/1‑94 (MQ=60)

                                                                                   TGTACCAATCCGCCCTAACATCGCCGCCAGCGTCCCCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATC           >  SRR4302536.4843439/1‑94 (MQ=60)

                                                                                   TGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATC           >  SRR4302536.4568646/1‑94 (MQ=60)

                                                                                     TACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAG         <  SRR4302536.3845216/94‑1 (MQ=60)

                                                                                      ACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGC        >  SRR4302536.2431524/1‑94 (MQ=60)

                                                                                       CCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCA       <  SRR4302536.4810489/94‑1 (MQ=60)

                                                                                       CCAACCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCA       <  SRR4302536.4224074/94‑1 (MQ=60)

                                                                                        CAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCAT      >  SRR4302536.6475195/1‑94 (MQ=60)

                                                                                         AATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATT     <  SRR4302536.196501/94‑1 (MQ=60)

                                                                                          ATCCGCCCTAACATCGCCGGCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGTGCTGAATTACCCAGACTGCGATCAGCATTG    >  SRR4302536.2538400/1‑94 (MQ=60)

                                                                                            CCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTG  <  SRR4302536.3264020/94‑1 (MQ=60)

GACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTG  >  NC_000913/1953809‑1953994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`