Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1475	266.7	13469680	98.2%	13227225	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,115,180	T→C	Q416R (CAG→CGG)	wcaK ←	putative galactokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,115,180	0	T	C	99.6%	819.3 / NA	251	Q416R (CAG→CGG)	wcaK	putative galactokinase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (157/93); minor base A (1/0); total (158/93)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 256 total aligned reads displayed.

GTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCA  >  NC_000913/2115090‑2115269

gTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGc                                                                                         >  2:2994388/1‑93 (MQ=255)

 tcaaGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCa                                                                                        >  1:6618385/3‑93 (MQ=255)

  tAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCAc                                                                                       <  2:3770348/93‑1 (MQ=255)

   aaGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCAcc                                                                                      <  1:5888679/93‑1 (MQ=255)

   aaGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCAcc                                                                                      >  1:289829/1‑93 (MQ=255)

   aaGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCAcc                                                                                      <  1:2284621/93‑1 (MQ=255)

     ggACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCAt                                                                                    <  1:3424048/93‑1 (MQ=255)

     ggACGAAGGTTTCTGACGACAGCGGGAATTTCAGAAAAAAGAACCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACACACCGCACCAt                                                                                    >  1:2942599/1‑93 (MQ=255)

       aCGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCt                                                                                  <  1:5658934/93‑1 (MQ=255)

       aCGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCt                                                                                  >  1:1462022/1‑93 (MQ=255)

       aCGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCt                                                                                  >  1:2512251/1‑93 (MQ=255)

          aaGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCa                                                                               >  1:3334319/1‑93 (MQ=255)

           aGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCAt                                                                              >  2:1124618/1‑93 (MQ=255)

           aGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCAt                                                                              >  1:138201/1‑93 (MQ=255)

           aGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCAt                                                                              >  1:4287987/1‑93 (MQ=255)

            ggTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCAtt                                                                             >  2:770883/1‑93 (MQ=255)

              tttCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTcc                                                                           >  1:6080839/1‑93 (MQ=255)

                tCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTg                                                                         <  1:2171829/93‑1 (MQ=255)

                    acgacAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTg                                                                     <  2:5745397/93‑1 (MQ=255)

                     cgacAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGa                                                                    >  1:2589381/1‑93 (MQ=255)

                     cgacAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGa                                                                    <  2:171008/93‑1 (MQ=255)

                     cgacAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGa                                                                    >  2:4883888/1‑93 (MQ=255)

                      gacAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGAc                                                                   <  1:4073675/93‑1 (MQ=255)

                        cAGCGGTAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGc                                                                 <  1:5465098/93‑1 (MQ=255)

                         aGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCt                                                                <  1:3045132/93‑1 (MQ=255)

                         aGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCt                                                                <  2:662592/93‑1 (MQ=255)

                          gCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTc                                                               >  1:6236032/1‑93 (MQ=255)

                          gCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTc                                                               <  1:1069685/93‑1 (MQ=255)

                           cGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCa                                                              >  2:5148229/1‑93 (MQ=255)

                            gggAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCAc                                                             >  1:1830027/1‑93 (MQ=255)

                            gggAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCAc                                                             >  1:3370490/1‑93 (MQ=255)

                             ggAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACg                                                            >  2:3908926/1‑93 (MQ=255)

                               aaTTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGAc                                                          <  1:2751166/93‑1 (MQ=255)

                                aTTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACt                                                         >  2:551385/1‑93 (MQ=255)

                                aTTTCAGTAAAAAGAAGACGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACt                                                         >  2:4426547/1‑93 (MQ=255)

                                 tttCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTg                                                        >  1:6702368/1‑93 (MQ=255)

                                 tttCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTg                                                        <  2:2388548/93‑1 (MQ=255)

                                 tttCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTg                                                        >  1:2456435/1‑93 (MQ=255)

                                   tCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGAc                                                      >  1:3749545/1‑93 (MQ=255)

                                      gTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGc                                                   >  1:4963814/1‑93 (MQ=255)

                                      gTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGc                                                   <  1:2078235/93‑1 (MQ=255)

                                       tAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCt                                                  <  2:5575021/93‑1 (MQ=255)

                                        aaaaaGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCtt                                                 <  1:4710594/93‑1 (MQ=255)

                                         aaaaGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTc                                                >  2:8410/1‑93 (MQ=255)

                                           aagaagCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCAc                                              >  1:5073254/1‑93 (MQ=255)

                                            agaagCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACt                                             >  2:6417369/1‑93 (MQ=255)

                                             gaagCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTa                                            >  2:492805/1‑93 (MQ=255)

                                              aagCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTaa                                           >  1:5311340/1‑93 (MQ=255)

                                              aagCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTaa                                           <  2:1528070/93‑1 (MQ=255)

                                               agCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAg                                          <  1:361455/93‑1 (MQ=255)

                                               agCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAg                                          >  2:3304933/1‑93 (MQ=255)

                                               agCCGACCTTCATTTCACCTCCCCGATGCGATCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGCCGGCTTCACTAAg                                          >  2:4230264/1‑93 (MQ=255)

                                                gCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGt                                         >  2:3601586/1‑93 (MQ=255)

                                                gCCGACCGTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGt                                         >  1:5402255/1‑93 (MQ=255)

                                                  cGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCCTTCCTGTCTGACGCTCACGACCGACGGCGTCACCAAGttg                                       >  1:1160577/1‑91 (MQ=255)

                                                  cGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTcg                                       >  1:3848063/1‑93 (MQ=255)

                                                   gACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTcgc                                      >  2:2263278/1‑93 (MQ=255)

                                                   gACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTcgc                                      >  2:1963776/1‑93 (MQ=255)

                                                    aCCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTcgcg                                     <  1:5808785/93‑1 (MQ=255)

                                                    aCCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTcgcg                                     >  1:1605167/1‑93 (MQ=255)

                                                    aCCTTCATATCACCTCCCCGGTGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTcgcg                                     >  1:6570079/1‑93 (MQ=255)

                                                     ccTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCgcgc                                    >  1:976224/1‑93 (MQ=255)

                                                     ccTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCgcgc                                    >  1:5517099/1‑93 (MQ=255)

                                                     ccTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCgcgc                                    >  2:5068277/1‑93 (MQ=255)

                                                      cTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCa                                   >  2:3135636/1‑93 (MQ=255)

                                                       ttCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCAt                                  >  1:6367223/1‑93 (MQ=255)

                                                       ttCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCAt                                  >  1:513878/1‑93 (MQ=255)

                                                        tCATTTCCCCTCCCCGATGCGCTCAAGCCCAGGCCGCAACGTCTGCATTTCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCAtt                                 <  2:3517304/93‑1 (MQ=255)

                                                         cATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTc                                >  1:2822389/1‑93 (MQ=255)

                                                          aTTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCa                               <  2:2593578/93‑1 (MQ=255)

                                                          aTTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCa                               <  1:5216127/93‑1 (MQ=255)

                                                          aTTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCa                               >  1:2634888/1‑93 (MQ=255)

                                                            ttCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAgc                             <  2:2462431/93‑1 (MQ=255)

                                                            ttCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAgc                             >  2:2183662/1‑93 (MQ=255)

                                                            ttCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAgc                             <  1:6369792/93‑1 (MQ=255)

                                                             tCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAgcg                            >  2:2081807/1‑93 (MQ=255)

                                                             tCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAgcg                            >  2:1761114/1‑93 (MQ=255)

                                                              cACCCCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAgcgc                           >  1:697665/1‑93 (MQ=255)

                                                               cccTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGcc                          <  1:4601085/92‑1 (MQ=255)

                                                               aCCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGcc                          >  2:6065565/1‑93 (MQ=255)

                                                               aCCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGcc                          >  2:5961432/1‑93 (MQ=255)

                                                                 cTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCgg                        >  2:2768660/1‑93 (MQ=255)

                                                                  tcaccGATGCGTTCAAGCACAAACCGCACCATCTGCATTCCTGTCTTACGCTCACGACTGGCGGGTTCACTAAGTCGCGGATTCAGGGCCGGa                       >  1:5852072/4‑93 (MQ=255)

                                                                   ccccGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGaa                      >  2:2526159/1‑93 (MQ=255)

                                                                     ccGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGc                    >  1:4454585/1‑93 (MQ=255)

                                                                        aTGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTgg                 >  1:632041/1‑93 (MQ=255)

                                                                         tGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGc                >  1:3089227/1‑93 (MQ=255)

                                                                           cgcTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGGCt              >  1:3307458/1‑93 (MQ=255)

                                                                           cgcTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTCGCCt              >  2:1196689/1‑93 (MQ=255)

                                                                             cTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTaa            <  2:4292881/93‑1 (MQ=255)

                                                                               cAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAgg          >  1:889515/1‑93 (MQ=255)

                                                                                aaGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGt         >  2:4729705/1‑93 (MQ=255)

                                                                                aaGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGt         >  2:4592439/1‑93 (MQ=255)

                                                                                aaGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGt         <  1:4740819/93‑1 (MQ=255)

                                                                                aaGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGt         >  2:6258671/1‑93 (MQ=255)

                                                                                aaGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGt         >  2:1915224/1‑93 (MQ=255)

                                                                                 aGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTa        >  2:4009036/1‑93 (MQ=255)

                                                                                 aGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTa        >  1:17457/1‑93 (MQ=255)

                                                                                 aGCACAGACCGCACCACCCGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTa        <  1:1796050/93‑1 (MQ=255)

                                                                                      aGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGc   <  1:6109538/93‑1 (MQ=255)

GTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCA  >  NC_000913/2115090‑2115269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAACCA  >  NC_000913/2115088‑2115273

TGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCG                                                                                              >  SRR4302536.735364/1‑94 (MQ=60)

 GGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCACCGATGCGCTCAAGCACAGACCGC                                                                                             >  SRR4302536.6510235/1‑94 (MQ=60)

  GTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCA                                                                                            >  SRR4302536.1831169/1‑94 (MQ=60)

  GTCAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCA                                                                                            >  SRR4302536.6815155/1‑94 (MQ=60)

   TTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCAC                                                                                           <  SRR4302536.1117067/94‑1 (MQ=60)

    TAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACC                                                                                          >  SRR4302536.297531/1‑94 (MQ=60)

     AAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCA                                                                                         <  SRR4302536.2353901/94‑1 (MQ=60)

     AAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCA                                                                                         <  SRR4302536.6064050/94‑1 (MQ=60)

      AGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGAAAAAAGAACCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACACACCGCACCAT                                                                                        >  SRR4302536.3029360/1‑94 (MQ=60)

       GGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATC                                                                                       <  SRR4302536.1634391/94‑1 (MQ=60)

       GGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATC                                                                                       <  SRR4302536.3525963/94‑1 (MQ=60)

        GACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCT                                                                                      >  SRR4302536.1505278/1‑94 (MQ=60)

        GACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCT                                                                                      >  SRR4302536.2588002/1‑94 (MQ=60)

         ACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTG                                                                                     <  SRR4302536.5827120/94‑1 (MQ=60)

         ACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTG                                                                                     >  SRR4302536.2094085/1‑94 (MQ=60)

          CGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGC                                                                                    >  SRR4302536.5645813/1‑94 (MQ=60)

           GAAGGTTTCTGACGACAGCGGGAATTTCAGTTAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGTACCATCTGCA                                                                                   >  SRR4302536.602180/1‑94 (MQ=60)

           GAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCA                                                                                   >  SRR4302536.3953576/1‑94 (MQ=60)

           GAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCA                                                                                   >  SRR4302536.3433048/1‑94 (MQ=60)

           GAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGAGCTCAAGCACAGACCGCACCATCTGCA                                                                                   >  SRR4302536.3120792/1‑94 (MQ=60)

           GAAGGTTTCTGACGACAGCGGGAAGTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGCTGCGCTCTAGCACATACCGCACCATCTGCA                                                                                   >  SRR4302536.2526727/1‑94 (MQ=60)

            AAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCAT                                                                                  >  SRR4302536.141810/1‑94 (MQ=60)

            AAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCAT                                                                                  >  SRR4302536.4416093/1‑94 (MQ=60)

               GTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCC                                                                               >  SRR4302536.2550448/1‑94 (MQ=60)

               GTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCC                                                                               >  SRR4302536.6262227/1‑94 (MQ=60)

               GTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCC                                                                               <  SRR4302536.338749/94‑1 (MQ=60)

                  TCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGT                                                                            <  SRR4302536.2237228/94‑1 (MQ=60)

                   CTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCCCAAGCACAGACCGCACCCTCTGCATTCCTtgc                                                                           >  SRR4302536.1646381/1‑91 (MQ=60)

                    TGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCT                                                                          >  SRR4302536.4737314/1‑94 (MQ=60)

                    TGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCT                                                                          <  SRR4302536.655768/94‑1 (MQ=60)

                    TGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCT                                                                          <  SRR4302536.1773375/94‑1 (MQ=60)

                    TGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCT                                                                          <  SRR4302536.2648026/94‑1 (MQ=60)

                    TGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGACT                                                                          <  SRR4302536.4328962/94‑1 (MQ=60)

                      GCGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCCCCATCTGCAATCCTGTCTGA                                                                        <  SRR4302536.805505/94‑1 (MQ=60)

                      ACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGA                                                                        >  SRR4302536.2667151/1‑94 (MQ=60)

                      ACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGA                                                                        >  SRR4302536.4866183/1‑94 (MQ=60)

                       CGACAGCGGGAATTTCAATAAAAAGAAGCCGCCCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGAC                                                                       <  SRR4302536.2691794/94‑1 (MQ=60)

                        GACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACG                                                                      <  SRR4302536.4194411/94‑1 (MQ=60)

                         ACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGC                                                                     <  SRR4302536.4558180/94‑1 (MQ=60)

                          CAGCGGTAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCT                                                                    <  SRR4302536.5628428/94‑1 (MQ=60)

                          CAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCT                                                                    <  SRR4302536.1022688/94‑1 (MQ=60)

                           AGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTTACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTC                                                                   <  SRR4302536.3134617/94‑1 (MQ=60)

                           AGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTC                                                                   <  SRR4302536.2483253/94‑1 (MQ=60)

                           AGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTC                                                                   >  SRR4302536.5994348/1‑94 (MQ=60)

                           AGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTC                                                                   >  SRR4302536.6421815/1‑94 (MQ=60)

                           AGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTC                                                                   <  SRR4302536.3134616/94‑1 (MQ=60)

                            GCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCA                                                                  <  SRR4302536.1101671/94‑1 (MQ=60)

                            GCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCA                                                                  >  SRR4302536.5413459/1‑94 (MQ=60)

                             CGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCCCCTCCCCGATGCGCTCAAGCACAGACCGCCCCATCTGCATTCCTGTCTGACGCCCAC                                                                 <  SRR4302536.5292912/94‑1 (MQ=60)

                             CGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCAC                                                                 >  SRR4302536.3470614/1‑94 (MQ=60)

                             CGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCAC                                                                 >  SRR4302536.1885200/1‑94 (MQ=60)

                              GGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACG                                                                >  SRR4302536.2997787/1‑94 (MQ=60)

                              GGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACG                                                                >  SRR4302536.2312398/1‑94 (MQ=60)

                                 AATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACT                                                             >  SRR4302536.664904/1‑94 (MQ=60)

                                 AATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACT                                                             <  SRR4302536.2833069/94‑1 (MQ=60)

                                 AATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACT                                                             <  SRR4302536.2651037/94‑1 (MQ=60)

                                  ATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTG                                                            >  SRR4302536.6546242/1‑94 (MQ=60)

                                  ATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTG                                                            >  SRR4302536.6901430/1‑94 (MQ=60)

                                  ATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTG                                                            >  SRR4302536.1262970/1‑94 (MQ=60)

                                  ATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTG                                                            >  SRR4302536.2530607/1‑94 (MQ=60)

                                   TTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGA                                                           >  SRR4302536.6777407/1‑94 (MQ=60)

                                    TTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGAC                                                          >  SRR4302536.3861619/1‑94 (MQ=60)

                                     TCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACG                                                         >  SRR4302536.999886/1‑94 (MQ=60)

                                      CAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGG                                                        >  SRR4302536.4827212/1‑94 (MQ=60)

                                      CAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGG                                                        >  SRR4302536.432885/1‑94 (MQ=60)

                                      CAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGG                                                        >  SRR4302536.6884821/1‑94 (MQ=60)

                                       AGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGC                                                       >  SRR4302536.5112929/1‑94 (MQ=60)

                                        GTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCT                                                      <  SRR4302536.2140816/94‑1 (MQ=60)

                                        GTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCT                                                      <  SRR4302536.3503024/94‑1 (MQ=60)

                                        GTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACAGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGAT                                                      >  SRR4302536.6842310/1‑94 (MQ=60)

                                         TAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTT                                                     <  SRR4302536.585005/94‑1 (MQ=60)

                                          AAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTC                                                    <  SRR4302536.3536480/94‑1 (MQ=60)

                                          AAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTC                                                    <  SRR4302536.4852284/94‑1 (MQ=60)

                                          AAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTC                                                    <  SRR4302536.2172042/94‑1 (MQ=60)

                                           AAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCA                                                   <  SRR4302536.5970325/94‑1 (MQ=60)

                                            AAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGTACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCAC                                                  <  SRR4302536.5247271/94‑1 (MQ=60)

                                            AAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCAC                                                  <  SRR4302536.6263447/94‑1 (MQ=60)

                                            AAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCAC                                                  >  SRR4302536.5225130/1‑94 (MQ=60)

                                               GAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAA                                               >  SRR4302536.342250/1‑94 (MQ=60)

                                               GAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAA                                               >  SRR4302536.5469900/1‑94 (MQ=60)

                                                AAGCTGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAG                                              >  SRR4302536.716464/1‑94 (MQ=60)

                                                AAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAG                                              <  SRR4302536.1924901/94‑1 (MQ=60)

                                                AAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAG                                              >  SRR4302536.1267619/1‑94 (MQ=60)

                                                AAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAG                                              >  SRR4302536.72105/1‑94 (MQ=60)

                                                 AGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGT                                             >  SRR4302536.3160266/1‑94 (MQ=60)

                                                 AGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGT                                             <  SRR4302536.371015/94‑1 (MQ=60)

                                                 AGCCGACCGTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGT                                             >  SRR4302536.5563630/1‑94 (MQ=60)

                                                   CCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCCTTCCTGTCTGACGCTCACGACCGACGGCGTCACCAAGTTG                                           >  SRR4302536.1195519/1‑94 (MQ=60)

                                                   CCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCG                                           >  SRR4302536.4618984/1‑94 (MQ=60)

                                                   CCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCG                                           <  SRR4302536.5551441/94‑1 (MQ=60)

                                                   CCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCG                                           >  SRR4302536.3962621/1‑94 (MQ=60)

                                                     GACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCG                                         >  SRR4302536.1652006/1‑94 (MQ=60)

                                                     GACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCG                                         >  SRR4302536.518909/1‑94 (MQ=60)

                                                     GACCTTCATATCACCTCCCCGGTGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCG                                         >  SRR4302536.6765601/1‑94 (MQ=60)

                                                      ACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGC                                        >  SRR4302536.1005035/1‑94 (MQ=60)

                                                      ACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGC                                        >  SRR4302536.5681632/1‑94 (MQ=60)

                                                      ACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGC                                        <  SRR4302536.5981802/94‑1 (MQ=60)

                                                       CCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCA                                       >  SRR4302536.2725781/1‑94 (MQ=60)

                                                        CTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCAT                                      >  SRR4302536.527062/1‑94 (MQ=60)

                                                        CTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCAT                                      <  SRR4302536.1849677/94‑1 (MQ=60)

                                                        CTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCAT                                      >  SRR4302536.6556959/1‑94 (MQ=60)

                                                         TTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATT                                     <  SRR4302536.2751830/94‑1 (MQ=60)

                                                          TCATTTCGCCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTC                                    <  SRR4302536.381566/94‑1 (MQ=60)

                                                          TCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTC                                    >  SRR4302536.2905970/1‑94 (MQ=60)

                                                           CATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCA                                   <  SRR4302536.6492285/94‑1 (MQ=60)

                                                           CATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCA                                   <  SRR4302536.5279161/94‑1 (MQ=60)

                                                           CATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCA                                   >  SRR4302536.4041674/1‑94 (MQ=60)

                                                           CATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCA                                   >  SRR4302536.2713906/1‑94 (MQ=60)

                                                            ATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAG                                  <  SRR4302536.2949429/94‑1 (MQ=60)

                                                            ATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAG                                  <  SRR4302536.5371572/94‑1 (MQ=60)

                                                              TTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCG                                <  SRR4302536.6559615/94‑1 (MQ=60)

                                                               TCACCCCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGC                               >  SRR4302536.718044/1‑94 (MQ=60)

                                                                CACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCC                              >  SRR4302536.600848/1‑94 (MQ=60)

                                                                 CCCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCG                             <  SRR4302536.4739263/94‑1 (MQ=60)

                                                                 CCCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCG                             <  SRR4302536.5754590/94‑1 (MQ=60)

                                                                  CCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGG                            <  SRR4302536.5770554/94‑1 (MQ=60)

                                                                  CCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGG                            >  SRR4302536.2861940/1‑94 (MQ=60)

                                                                   CTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGA                           >  SRR4302536.5052584/1‑94 (MQ=60)

                                                                   CTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGA                           >  SRR4302536.3466247/1‑94 (MQ=60)

                                                                   CTCACCGATGCGTTCAAGCACAAACCGCACCATCTGCATTCCTGTCTTACGCTCACGACTGGCGGGTTCACTAAGTCGCGGATTCAGGGCCGGA                           >  SRR4302536.6026425/1‑94 (MQ=55)

                                                                     CCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAG                         >  SRR4302536.4899647/1‑94 (MQ=60)

                                                                     CCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAG                         >  SRR4302536.3871305/1‑94 (MQ=60)

                                                                      CCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGC                        >  SRR4302536.1867262/1‑94 (MQ=60)

                                                                      CCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGC                        >  SRR4302536.4588406/1‑94 (MQ=60)

                                                                      CCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGC                        <  SRR4302536.6368996/94‑1 (MQ=60)

                                                                       CCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCT                       <  SRR4302536.2178278/94‑1 (MQ=60)

                                                                        CGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTG                      >  SRR4302536.5571463/1‑94 (MQ=60)

                                                                        CGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTG                      <  SRR4302536.2945889/94‑1 (MQ=60)

                                                                        CGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTGCGCTAAGGCGAGCATTCAGCGCCCGACGCTG                      >  SRR4302536.2245810/1‑94 (MQ=60)

                                                                         GATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGG                     >  SRR4302536.650746/1‑94 (MQ=60)

                                                                         GATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGG                     <  SRR4302536.3022236/94‑1 (MQ=60)

                                                                          ATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGC                    >  SRR4302536.1459503/1‑94 (MQ=60)

                                                                          ATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGC                    >  SRR4302536.3179554/1‑94 (MQ=60)

                                                                          ATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGC                    >  SRR4302536.485521/1‑94 (MQ=60)

                                                                           TGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCC                   >  SRR4302536.6618688/1‑94 (MQ=60)

                                                                            GCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGGCT                  >  SRR4302536.3405114/1‑94 (MQ=60)

                                                                              GCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAA                <  SRR4302536.55583/94‑1 (MQ=60)

                                                                               CTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAG               >  SRR4302536.6208843/1‑94 (MQ=60)

                                                                                TCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGG              >  SRR4302536.3636869/1‑94 (MQ=60)

                                                                                TCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGG              >  SRR4302536.915714/1‑94 (MQ=60)

                                                                                TCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGG              >  SRR4302536.542917/1‑94 (MQ=60)

                                                                                 CAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGT             <  SRR4302536.3473706/94‑1 (MQ=60)

                                                                                 CAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGT             >  SRR4302536.6267334/1‑94 (MQ=60)

                                                                                  AAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTA            <  SRR4302536.4883472/94‑1 (MQ=60)

                                                                                  AAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTA            >  SRR4302536.17941/1‑94 (MQ=60)

                                                                                   AGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTAT           <  SRR4302536.6485134/94‑1 (MQ=60)

                                                                                   AGCACAGACCGCACCACCCGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTAT           <  SRR4302536.1850272/94‑1 (MQ=60)

                                                                                        AGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCA      >  SRR4302536.3416497/1‑94 (MQ=60)

                                                                                        AGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCA      >  SRR4302536.1006869/1‑94 (MQ=60)

                                                                                        AGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCA      <  SRR4302536.6291663/94‑1 (MQ=60)

                                                                                          ACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAAC    >  SRR4302536.5919787/1‑94 (MQ=60)

                                                                                           CCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAACC   >  SRR4302536.3748540/1‑94 (MQ=60)

                                                                                            CGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAACCA  >  SRR4302536.6871548/1‑94 (MQ=60)

                                                                                            CGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAACCA  <  SRR4302536.3173563/94‑1 (MQ=60)

TGGTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCAACCA  >  NC_000913/2115088‑2115273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`