Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,429,527 Δ1 bp coding (40/510 nt) yrdD ← putative DNA topoisomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,429,5260C.100.0% 1083.5 / NA 233coding (41/510 nt)yrdDputative DNA topoisomerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (112/121);  total (112/121)

Only 100 of 254 total aligned reads displayed.

CGATATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATCCGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTG  >  NC_000913/3429434‑3429614
                                                                                            |                                                                                        
cGATATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATc                                                                                          >  2:5578669/1‑93 (MQ=255)
 gATATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATcg                                                                                         <  2:5152994/93‑2 (MQ=255)
  atatGTCCATCCGCTGAAGCTTTAAGAGGACGGACGTAGTCACACTCCGGATACTGTTAGCATCCAAGAAACGGACCGTGTTTCCCGGATcga                                                                                        >  2:946410/1‑91 (MQ=255)
  atatGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATcga                                                                                        >  1:5843246/1‑91 (MQ=255)
   tatGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATcgaa                                                                                       >  1:6734654/1‑90 (MQ=255)
    atGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATcgaat                                                                                      <  1:6459889/93‑5 (MQ=255)
      gTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATaa                                                                                   <  2:3105383/93‑1 (MQ=255)
       tCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAAc                                                                                  >  1:367000/1‑93 (MQ=255)
       tCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAAc                                                                                  >  1:3220386/1‑93 (MQ=255)
        ccATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAAcc                                                                                 <  2:4048041/93‑1 (MQ=255)
        ccATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAAcc                                                                                 <  1:4568386/93‑1 (MQ=255)
         cATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCa                                                                                <  1:6685295/93‑1 (MQ=255)
         cATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCa                                                                                <  2:5275498/93‑1 (MQ=255)
            ccGCGGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGtt                                                                             <  1:820662/93‑1 (MQ=255)
             cGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTc                                                                            <  2:5827259/93‑1 (MQ=255)
              gCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCa                                                                           <  1:4527857/93‑1 (MQ=255)
                 gAAGATTTCAGAGGGCGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGcc                                                                        >  2:2784794/1‑93 (MQ=255)
                 gAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGcc                                                                        <  2:6305070/93‑1 (MQ=255)
                     aTTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGc                                                                    <  2:1019616/93‑1 (MQ=255)
                      tttCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCa                                                                   >  2:6475145/1‑93 (MQ=255)
                       ttCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCAc                                                                  <  1:1222570/93‑1 (MQ=255)
                        tCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACt                                                                 >  1:1551047/1‑93 (MQ=255)
                        tCAGAGGACGGACGTAGCCCCACGCCGGATACTGTGAGCATCCAAGAAACGGACTGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACt                                                                 <  1:3930704/93‑1 (MQ=255)
                         cAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACtt                                                                <  1:3157527/93‑1 (MQ=255)
                         cAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACtt                                                                >  1:4175713/1‑93 (MQ=255)
                          agagGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACttt                                                               <  2:1990045/93‑1 (MQ=255)
                           gagGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTg                                                              <  1:5801171/93‑1 (MQ=255)
                            agGACGGACGTAGTCGCACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTgg                                                             <  2:2212482/93‑1 (MQ=255)
                            agGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTgg                                                             <  1:4766854/93‑1 (MQ=255)
                             ggacggacGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTggg                                                            <  1:5646495/93‑1 (MQ=255)
                              gacggacgTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGc                                                           <  1:1884592/93‑1 (MQ=255)
                               acggacgTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCa                                                          <  1:5420637/93‑1 (MQ=255)
                               acggacgTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCa                                                          >  2:3806100/1‑93 (MQ=255)
                                 ggacgTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAgg                                                        <  1:4287471/93‑1 (MQ=255)
                                   acgTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGAc                                                      <  2:147588/93‑1 (MQ=255)
                                   acgTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGAc                                                      >  1:1758629/1‑93 (MQ=255)
                                      tAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCa                                                   <  1:3853224/93‑1 (MQ=255)
                                      tAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCa                                                   <  1:5498258/93‑1 (MQ=255)
                                       aGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCat                                                  <  1:1471125/93‑1 (MQ=255)
                                        gTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCatt                                                 >  2:4337800/1‑93 (MQ=255)
                                         tCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCatta                                                <  1:240026/93‑1 (MQ=255)
                                          cacaCGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCattat                                               >  1:6270793/1‑93 (MQ=255)
                                           acacGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCattatt                                              <  1:2679542/93‑1 (MQ=255)
                                           acacGCCGGATACTGCGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTAAGCGCCCCACTTTGGGCAGGACTCattatt                                              >  1:3455477/1‑93 (MQ=255)
                                            cacGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCAttatta                                             <  1:5236610/93‑1 (MQ=255)
                                             acGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTAc                                            <  2:1676717/93‑1 (MQ=255)
                                             acGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTAc                                            >  2:5186055/1‑93 (MQ=255)
                                             acGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTAc                                            >  2:3855850/1‑93 (MQ=255)
                                                 cGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCAc                                        >  2:1626448/1‑93 (MQ=255)
                                                  ggATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCAcc                                       <  1:4915551/93‑1 (MQ=255)
                                                  ggATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCAcc                                       <  1:2043008/93‑1 (MQ=255)
                                                      aCTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGa                                   <  2:1577810/93‑1 (MQ=255)
                                                        tgtgAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAAc                                 >  1:3895647/1‑93 (MQ=255)
                                                         gtgAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACa                                <  2:735390/93‑1 (MQ=255)
                                                         gtgAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACa                                <  1:1025361/93‑1 (MQ=255)
                                                          tgAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAg                               >  2:4721323/1‑93 (MQ=255)
                                                          tgAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAg                               >  2:4983891/1‑93 (MQ=255)
                                                          tgAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAg                               >  1:2168874/1‑93 (MQ=255)
                                                          tgAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAg                               <  2:3430146/93‑1 (MQ=255)
                                                           gAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGt                              >  2:4173430/1‑93 (MQ=255)
                                                           gAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGt                              <  1:2931015/93‑1 (MQ=255)
                                                            aGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTg                             <  1:896978/93‑1 (MQ=255)
                                                             gCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGc                            >  2:1215247/1‑93 (MQ=255)
                                                             gCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGc                            >  2:6387759/1‑93 (MQ=255)
                                                             gCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGc                            >  2:2522837/1‑93 (MQ=255)
                                                                tCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGa                         >  1:4448216/1‑93 (MQ=255)
                                                                tCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGa                         >  1:2605472/1‑93 (MQ=255)
                                                                 ccAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGAt                        <  1:2539321/93‑1 (MQ=255)
                                                                 ccAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGAt                        <  2:2600902/93‑1 (MQ=255)
                                                                   aaGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGAttt                      >  2:4530914/1‑93 (MQ=255)
                                                                    aGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTATGCACCGTGAACAGTGCTGATTTc                     >  1:5507805/1‑93 (MQ=255)
                                                                     gAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCg                    <  1:4037747/93‑1 (MQ=255)
                                                                     gAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCg                    <  2:5755008/93‑1 (MQ=255)
                                                                       aaCGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGcc                  >  2:599888/1‑93 (MQ=255)
                                                                       aaCGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGcc                  >  1:1687322/1‑93 (MQ=255)
                                                                       aaCGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGcc                  >  2:6722378/1‑93 (MQ=255)
                                                                        aCGGACCGTGTTTCCCGGAT‑CGAATAATCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCa                 <  2:660544/93‑1 (MQ=255)
                                                                        aCGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCa                 >  1:4607224/1‑93 (MQ=255)
                                                                         cGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCAt                >  2:460557/1‑93 (MQ=255)
                                                                          ggACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATa               >  2:6101281/1‑93 (MQ=255)
                                                                          ggACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATa               >  2:4044612/1‑93 (MQ=255)
                                                                          ggACCGTGGTTCCCGGAT‑CGAGTAACCAGTTCAGCTCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATa               <  1:1050671/93‑1 (MQ=255)
                                                                           gACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATaa              <  1:5629733/93‑1 (MQ=255)
                                                                           gACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATaa              >  1:297764/1‑93 (MQ=255)
                                                                           gACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATaa              <  2:5370799/93‑1 (MQ=255)
                                                                             ccGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATaaca            >  1:4996337/1‑93 (MQ=255)
                                                                              cGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATaacaa           <  2:3279060/93‑1 (MQ=255)
                                                                               gtgtTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATaacaac          <  1:2344533/93‑1 (MQ=255)
                                                                               gtgtTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATaacaac          >  1:6026338/1‑93 (MQ=255)
                                                                               gtgtTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATaacaac          <  2:186920/93‑1 (MQ=255)
                                                                               gtgtTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATaacaac          >  1:115169/1‑93 (MQ=255)
                                                                                tgtTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACt         >  1:2507173/1‑93 (MQ=255)
                                                                                  tttCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTa       <  1:3310900/93‑1 (MQ=255)
                                                                                  tttCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTa       <  2:4731351/93‑1 (MQ=255)
                                                                                    tCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATg     >  2:3356827/1‑93 (MQ=255)
                                                                                     cccGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGc    >  1:5335729/1‑93 (MQ=255)
                                                                                     cccGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGc    >  2:4469704/1‑93 (MQ=255)
                                                                                     cccGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGc    <  2:2877203/93‑1 (MQ=255)
                                                                                      ccGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCt   <  1:5126006/93‑1 (MQ=255)
                                                                                       cGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTg  >  2:4893653/1‑93 (MQ=255)
                                                                                            |                                                                                        
CGATATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATCCGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTG  >  NC_000913/3429434‑3429614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATCCGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAATTAA  >  NC_000913/3429436‑3429620
                                                                                           |                                                                                             
ATATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAA                                                                                            >  SRR4302536.6935057/1‑94 (MQ=60)
 TATGTCCATCCGCTGAAGATTGCAGAGAACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAAT                                                                                           <  SRR4302536.2115979/94‑1 (MQ=60)
  ATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATA                                                                                          <  SRR4302536.6652748/94‑1 (MQ=60)
  ATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATA                                                                                          >  SRR4302536.2540001/1‑94 (MQ=60)
  ATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATA                                                                                          >  SRR4302536.4706205/1‑94 (MQ=60)
    GTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAAC                                                                                        >  SRR4302536.3314820/1‑94 (MQ=60)
    GTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAAC                                                                                        >  SRR4302536.376700/1‑94 (MQ=60)
     TCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACC                                                                                       >  SRR4302536.3824098/1‑94 (MQ=60)
      CCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCA                                                                                      <  SRR4302536.2470937/94‑1 (MQ=60)
      CCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCA                                                                                      <  SRR4302536.4705476/94‑1 (MQ=60)
       CATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAG                                                                                     <  SRR4302536.6883852/94‑1 (MQ=60)
          CCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTC                                                                                  >  SRR4302536.4056495/1‑94 (MQ=60)
          CCGCGGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTC                                                                                  <  SRR4302536.844811/94‑1 (MQ=60)
           CGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCA                                                                                 >  SRR4302536.3991683/1‑94 (MQ=60)
           CGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCA                                                                                 <  SRR4302536.342708/94‑1 (MQ=60)
           CGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCA                                                                                 >  SRR4302536.2520438/1‑94 (MQ=60)
            GCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAG                                                                                <  SRR4302536.4663606/94‑1 (MQ=60)
                 AGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCG                                                                           >  SRR4302536.4028836/1‑94 (MQ=60)
                     TTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACT                                                                       <  SRR4302536.1549601/94‑1 (MQ=60)
                     TTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACT                                                                       >  SRR4302536.1596631/1‑94 (MQ=60)
                     TTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACT                                                                       <  SRR4302536.1259310/94‑1 (MQ=60)
                     TTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACT                                                                       <  SRR4302536.3041854/94‑1 (MQ=60)
                      TCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTT                                                                      >  SRR4302536.4299767/1‑94 (MQ=60)
                      TCAGAGGACGGACGTAGCCCCACGCCGGATACTGTGAGCATCCAAGAAACGGACTGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTT                                                                      <  SRR4302536.4047500/94‑1 (MQ=60)
                       CAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTT                                                                     <  SRR4302536.3250014/94‑1 (MQ=60)
                        AGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTG                                                                    <  SRR4302536.5807513/94‑1 (MQ=60)
                         GAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGG                                                                   <  SRR4302536.5973899/94‑1 (MQ=60)
                          AGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGG                                                                  <  SRR4302536.4910413/94‑1 (MQ=60)
                          AGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCACGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGG                                                                  >  SRR4302536.969279/1‑94 (MQ=60)
                           GGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGC                                                                 <  SRR4302536.5814279/94‑1 (MQ=60)
                            GACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCA                                                                <  SRR4302536.1941285/94‑1 (MQ=60)
                             ACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAG                                                               <  SRR4302536.1187878/94‑1 (MQ=60)
                             ACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAG                                                               <  SRR4302536.5582667/94‑1 (MQ=60)
                               GGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGA                                                             >  SRR4302536.477352/1‑94 (MQ=60)
                               GGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGA                                                             <  SRR4302536.4415548/94‑1 (MQ=60)
                                GACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGAC                                                            >  SRR4302536.1811253/1‑94 (MQ=60)
                                GACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGAC                                                            >  SRR4302536.1899300/1‑94 (MQ=60)
                                   GTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCA                                                         <  SRR4302536.1597130/94‑1 (MQ=60)
                                   GTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCA                                                         <  SRR4302536.1107880/94‑1 (MQ=60)
                                   GTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCA                                                         <  SRR4302536.261497/94‑1 (MQ=60)
                                    TAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCAT                                                        <  SRR4302536.3967923/94‑1 (MQ=60)
                                    TAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCAT                                                        <  SRR4302536.5662317/94‑1 (MQ=60)
                                    TAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCAT                                                        <  SRR4302536.5768199/94‑1 (MQ=60)
                                    TAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCAT                                                        <  SRR4302536.3371226/94‑1 (MQ=60)
                                    TAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCAT                                                        <  SRR4302536.929043/94‑1 (MQ=60)
                                     AGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATT                                                       <  SRR4302536.1514644/94‑1 (MQ=60)
                                       TCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTAT                                                     <  SRR4302536.2286468/94‑1 (MQ=60)
                                       TCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTAT                                                     >  SRR4302536.4928389/1‑94 (MQ=60)
                                       TCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTAT                                                     <  SRR4302536.246418/94‑1 (MQ=60)
                                       TCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTAT                                                     >  SRR4302536.6457628/1‑94 (MQ=60)
                                        CACACGCCGGATACTGCGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTAAGCGCCCCACTTTGGGCAGGACTCATTATT                                                    >  SRR4302536.3558401/1‑94 (MQ=60)
                                         ACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTA                                                   <  SRR4302536.2759620/94‑1 (MQ=60)
                                         ACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTA                                                   <  SRR4302536.1656318/94‑1 (MQ=60)
                                          CACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTAC                                                  <  SRR4302536.3216347/94‑1 (MQ=60)
                                          CACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTAC                                                  <  SRR4302536.5392630/94‑1 (MQ=60)
                                           ACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACG                                                 >  SRR4302536.5355890/1‑94 (MQ=60)
                                               CGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACC                                             <  SRR4302536.1889988/94‑1 (MQ=60)
                                                GGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCG                                            <  SRR4302536.2104518/94‑1 (MQ=60)
                                                GGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCG                                            <  SRR4302536.5063290/94‑1 (MQ=60)
                                                GGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCG                                            <  SRR4302536.5631439/94‑1 (MQ=60)
                                                    ACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAA                                        >  SRR4302536.3721624/1‑94 (MQ=60)
                                                    ACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAA                                        <  SRR4302536.2418306/94‑1 (MQ=60)
                                                     CTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTTAGCCCCGCACTCTGGGCAGGACTCATTATTACGCACCGTGAAC                                       >  SRR4302536.3695783/1‑94 (MQ=60)
                                                     CTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAAC                                       >  SRR4302536.4011475/1‑94 (MQ=60)
                                                     CTGTGAACATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACTCTCCGTGAAC                                       <  SRR4302536.1924225/94‑1 (MQ=60)
                                                      TGTTAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAAGAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACA                                      <  SRR4302536.4019674/94‑1 (MQ=60)
                                                      TGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACA                                      <  SRR4302536.4899815/94‑1 (MQ=60)
                                                       GTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAG                                     <  SRR4302536.4307789/94‑1 (MQ=60)
                                                       GTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAG                                     >  SRR4302536.2234161/1‑94 (MQ=60)
                                                       GTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAG                                     <  SRR4302536.1055724/94‑1 (MQ=60)
                                                       GTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAG                                     <  SRR4302536.2151304/94‑1 (MQ=60)
                                                        TGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGT                                    <  SRR4302536.688685/94‑1 (MQ=60)
                                                        TGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCACGCACTTTGGGCAGGACTCATTATTGCGCACCGTGAACAGT                                    >  SRR4302536.5063900/1‑94 (MQ=60)
                                                         GAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTG                                   <  SRR4302536.3017501/94‑1 (MQ=60)
                                                          AGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGC                                  >  SRR4302536.5418363/1‑94 (MQ=60)
                                                          AGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGC                                  >  SRR4302536.6659983/1‑94 (MQ=60)
                                                          AGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGC                                  <  SRR4302536.923411/94‑1 (MQ=60)
                                                          AGCATCCAAGAAACAGAACGTGTTTCCAGGATCCG‑ATGACCAGTTCAGGCCCGCACTTAGGGCAGGACTCATTATTACGCACCGTGCACAGTGC                                  >  SRR4302536.1097968/1‑94 (MQ=59)
                                                           GCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCT                                 <  SRR4302536.5647639/94‑1 (MQ=60)
                                                           GCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCT                                 >  SRR4302536.1285468/1‑94 (MQ=60)
                                                           GCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCA                                 <  SRR4302536.1179739/94‑1 (MQ=60)
                                                           GCATCCAAGAAACCGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCT                                 <  SRR4302536.5667124/94‑1 (MQ=60)
                                                            CATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATCACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTG                                <  SRR4302536.1741868/94‑1 (MQ=60)
                                                            CATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCATTTCCGCACTTTGGGCAGGCCTCATTATTACGCCCCGTGCACAGTGCTG                                >  SRR4302536.6503404/1‑94 (MQ=60)
                                                             ATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGA                               >  SRR4302536.4581800/1‑94 (MQ=60)
                                                             ATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGA                               >  SRR4302536.3248740/1‑94 (MQ=60)
                                                             ATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGA                               >  SRR4302536.2683676/1‑94 (MQ=60)
                                                             ATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGA                               >  SRR4302536.5285806/1‑94 (MQ=60)
                                                              TCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGAT                              <  SRR4302536.827186/94‑1 (MQ=60)
                                                               CCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATT                             >  SRR4302536.6526486/1‑94 (MQ=60)
                                                               CCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATT                             >  SRR4302536.6824213/1‑94 (MQ=60)
                                                               CCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATT                             >  SRR4302536.4518905/1‑94 (MQ=60)
                                                               CCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATT                             <  SRR4302536.2615825/94‑1 (MQ=60)
                                                                CAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTT                            <  SRR4302536.723509/94‑1 (MQ=60)
                                                                 AAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTATGCACCGTGAACAGTGCTGATTTC                           >  SRR4302536.5672109/1‑94 (MQ=60)
                                                                 AAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTC                           <  SRR4302536.2607437/94‑1 (MQ=60)
                                                                  AGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCG                          >  SRR4302536.5983876/1‑94 (MQ=60)
                                                                  AGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATGTCG                          >  SRR4302536.5161978/1‑94 (MQ=60)
                                                                   GAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGC                         <  SRR4302536.4157466/94‑1 (MQ=60)
                                                                    AAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCTCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGCTTTCGCC                        >  SRR4302536.2862059/1‑94 (MQ=60)
                                                                    AAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCC                        >  SRR4302536.1736207/1‑94 (MQ=60)
                                                                    AAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCC                        <  SRR4302536.5704269/94‑1 (MQ=60)
                                                                    AAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCC                        >  SRR4302536.592304/1‑94 (MQ=60)
                                                                    AAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCC                        >  SRR4302536.5110955/1‑94 (MQ=60)
                                                                    AAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCC                        >  SRR4302536.3337512/1‑94 (MQ=60)
                                                                     AACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCA                       >  SRR4302536.4745605/1‑94 (MQ=60)
                                                                        GGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAA                    <  SRR4302536.5449092/94‑1 (MQ=60)
                                                                        GGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAA                    >  SRR4302536.305674/1‑94 (MQ=60)
                                                                        GGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAA                    >  SRR4302536.4286797/1‑94 (MQ=60)
                                                                        GGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAA                    >  SRR4302536.1429349/1‑94 (MQ=60)
                                                                        GGACCGTGGTTCCCGGAT‑CGAGTAACCAGTTCAGCTCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAA                    <  SRR4302536.1082026/94‑1 (MQ=60)
                                                                         GACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAAC                   <  SRR4302536.2732702/94‑1 (MQ=60)
                                                                         GACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAAC                   >  SRR4302536.6439719/1‑94 (MQ=60)
                                                                         GACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAAC                   <  SRR4302536.6144630/94‑1 (MQ=60)
                                                                         GACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAAC                   <  SRR4302536.5796976/94‑1 (MQ=60)
                                                                          ACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACA                  >  SRR4302536.5146290/1‑94 (MQ=60)
                                                                            CGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAAC                <  SRR4302536.6074778/94‑1 (MQ=60)
                                                                            CGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAAC                >  SRR4302536.118223/1‑94 (MQ=60)
                                                                            CGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAAC                >  SRR4302536.465550/1‑94 (MQ=60)
                                                                            CGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAAC                >  SRR4302536.6206015/1‑94 (MQ=60)
                                                                             GTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACT               <  SRR4302536.2415636/94‑1 (MQ=60)
                                                                             GTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACT               >  SRR4302536.3975691/1‑94 (MQ=60)
                                                                             GTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACT               >  SRR4302536.2582767/1‑94 (MQ=60)
                                                                             GTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACT               <  SRR4302536.2887006/94‑1 (MQ=60)
                                                                               GTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTA             <  SRR4302536.3280815/94‑1 (MQ=60)
                                                                               GTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTA             <  SRR4302536.4166385/94‑1 (MQ=60)
                                                                                TTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTAT            <  SRR4302536.3408713/94‑1 (MQ=60)
                                                                                TTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTAT            <  SRR4302536.194374/94‑1 (MQ=60)
                                                                                 TTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATG           <  SRR4302536.5495552/94‑1 (MQ=60)
                                                                                 TTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATG           >  SRR4302536.1064721/1‑94 (MQ=60)
                                                                                  TCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGC          >  SRR4302536.5495162/1‑94 (MQ=60)
                                                                                  TCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGC          <  SRR4302536.4428350/94‑1 (MQ=60)
                                                                                   CCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCT         <  SRR4302536.862117/94‑1 (MQ=60)
                                                                                    CCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTG        <  SRR4302536.5279159/94‑1 (MQ=60)
                                                                                    CCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTG        <  SRR4302536.2638486/94‑1 (MQ=60)
                                                                                    CCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTG        <  SRR4302536.4688197/94‑1 (MQ=60)
                                                                                    CCGGAT‑CGAATAACCAGTCGAGCCCCGCACTTAGGACAGGACTGATTATTACGCACCGTGAACAGTGCTGATTTCGCCAGAACATCTTATGCTG        <  SRR4302536.4633549/94‑1 (MQ=60)
                                                                                     CGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGA       <  SRR4302536.5941461/94‑1 (MQ=60)
                                                                                      GGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAA      <  SRR4302536.1255994/94‑1 (MQ=60)
                                                                                         atCGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAATT    <  SRR4302536.6046263/92‑1 (MQ=60)
                                                                                           CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAATTAA  >  SRR4302536.1249505/1‑94 (MQ=60)
                                                                                           CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAATTAA  >  SRR4302536.2666426/1‑94 (MQ=60)
                                                                                           CGAATAACCAGTCCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAATTAA  >  SRR4302536.3793426/1‑94 (MQ=60)
                                                                                           |                                                                                             
ATATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATCCGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAATTAA  >  NC_000913/3429436‑3429620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑