Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,540,000 T→C V653A (GTG→GCG)  feoB → ferrous iron transport protein B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,540,0000TC99.3% 946.9 / ‑5.4 294V653A (GTG→GCG) feoBferrous iron transport protein B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (130/162);  minor base G (2/0);  total (132/162)
Fisher's exact test for biased strand distribution p-value = 2.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 307 total aligned reads displayed.

GCCTGAAAGACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGTGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGG  >  NC_000913/3539910‑3540089
                                                                                          |                                                                                         
 ccTGAAAGACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGAt                                                                                        <  1:5548089/93‑1 (MQ=255)
 ccTGAAAGACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGAt                                                                                        <  1:6431389/93‑1 (MQ=255)
  cTGAAAGACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATg                                                                                       <  1:1935312/93‑1 (MQ=255)
  cTGAAAGACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATg                                                                                       <  2:1593070/93‑1 (MQ=255)
     aaaGACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGAt                                                                                    >  2:4487429/1‑93 (MQ=255)
      aaGACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATc                                                                                   >  1:37322/1‑93 (MQ=255)
       aGACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCa                                                                                  >  1:1238923/1‑93 (MQ=255)
        gACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAg                                                                                 <  1:2123066/93‑1 (MQ=255)
         acacCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGa                                                                                >  1:1544356/1‑93 (MQ=255)
            ccTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAAt                                                                             <  2:2746438/93‑1 (MQ=255)
            ccTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAAt                                                                             <  2:1827422/93‑1 (MQ=255)
             cTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAAtt                                                                            <  1:5719569/93‑1 (MQ=255)
               tCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGCGTACCGGCGCGATTGGCGCGCTGGGTCACAAATTCg                                                                          >  1:5660298/1‑93 (MQ=255)
                 aGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGt                                                                        <  2:653627/93‑1 (MQ=255)
                  gCCTTCGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAGTGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTa                                                                       <  2:4723881/93‑1 (MQ=255)
                  gCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTa                                                                       <  1:6220616/93‑1 (MQ=255)
                  gCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTa                                                                       >  2:406464/1‑93 (MQ=255)
                     ttAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAgcg                                                                    >  1:4283170/1‑93 (MQ=255)
                     ttAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAgcg                                                                    >  1:4624328/1‑93 (MQ=255)
                      tAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAgcgc                                                                   >  2:118579/1‑93 (MQ=255)
                       aGCGTACTGATGAACCCCATTGAAGCCAGCATAGGCGACGGCGAAATGGGTACTGGGGCGATGGGCGCGATGGATAAGAAATTCGGTAGCgca                                                                  >  2:3219833/1‑93 (MQ=255)
                       aGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCgca                                                                  <  1:4985712/93‑1 (MQ=255)
                         cGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCgcagc                                                                <  2:5276543/93‑1 (MQ=255)
                              tgatgaACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGcagcagcag                                                           <  2:4640221/93‑1 (MQ=255)
                              tgatgaACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGcagcagcag                                                           >  1:2188915/1‑93 (MQ=255)
                              tgatgaACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGcagcagcag                                                           >  2:4459898/1‑93 (MQ=255)
                                atgaACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCt                                                         <  1:115218/93‑1 (MQ=255)
                                 tgaACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCtt                                                        <  2:3520494/93‑1 (MQ=255)
                                  gaACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTa                                                       >  1:3097936/1‑93 (MQ=255)
                                   aaCCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTAc                                                      <  1:2805434/93‑1 (MQ=255)
                                   aaCCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTAc                                                      <  2:4956199/93‑1 (MQ=255)
                                    aCCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACa                                                     <  1:1640696/93‑1 (MQ=255)
                                      cccATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGc                                                   >  1:3051992/1‑93 (MQ=255)
                                         aTTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTAc                                                >  2:4007439/1‑93 (MQ=255)
                                           tGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCt                                              <  1:1094369/93‑1 (MQ=255)
                                           tGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCt                                              <  2:6587738/93‑1 (MQ=255)
                                            gAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTg                                             >  1:4866584/1‑93 (MQ=255)
                                            gAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTg                                             <  2:5483367/93‑1 (MQ=255)
                                             aaGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGa                                            >  2:4127902/1‑93 (MQ=255)
                                             aaGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGa                                            <  2:3140170/93‑1 (MQ=255)
                                              aGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGAt                                           >  1:711056/1‑93 (MQ=255)
                                               gCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGAtt                                          >  1:5777968/1‑93 (MQ=255)
                                               gCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGAtt                                          <  1:5948544/93‑1 (MQ=255)
                                               gCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGAtt                                          >  1:1005709/1‑93 (MQ=255)
                                               gCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGAtt                                          <  1:3968944/93‑1 (MQ=255)
                                                ccAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGAttt                                         >  2:1052745/1‑93 (MQ=255)
                                                 cAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGAtttt                                        >  1:3842609/1‑93 (MQ=255)
                                                   gCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGGTCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCg                                      >  2:108176/1‑93 (MQ=255)
                                                     aaaGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTc                                    >  1:926147/1‑93 (MQ=255)
                                                     aaaGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTc                                    <  2:5906505/93‑1 (MQ=255)
                                                      aaGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTcc                                   <  1:174578/93‑1 (MQ=255)
                                                      aaGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTcc                                   >  1:3649854/1‑93 (MQ=255)
                                                       aGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCct                                  <  1:2374308/93‑1 (MQ=255)
                                                       aGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCct                                  <  2:1135890/93‑1 (MQ=255)
                                                       aGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCct                                  >  1:4413418/1‑93 (MQ=255)
                                                       aGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCct                                  >  1:6442865/1‑93 (MQ=255)
                                                       aGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCct                                  <  1:3207396/93‑1 (MQ=255)
                                                       aGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCct                                  <  2:6234240/93‑1 (MQ=255)
                                                         gctaCGGAGAAATGGGTACCGGGGCTATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCctgc                                <  1:2614544/90‑1 (MQ=255)
                                                         gCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTCGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCctgc                                <  1:3494287/93‑1 (MQ=255)
                                                         gCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCctgc                                >  1:3141466/1‑93 (MQ=255)
                                                         gCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCctgc                                <  1:938027/93‑1 (MQ=255)
                                                           gACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCctgctg                              >  1:328352/1‑93 (MQ=255)
                                                           gACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCctgctg                              <  1:5402732/93‑1 (MQ=255)
                                                            aCGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCtgt                             <  2:3709273/93‑1 (MQ=255)
                                                             cGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCtgta                            <  2:678923/93‑1 (MQ=255)
                                                              ggCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCtgtat                           <  2:5571923/93‑1 (MQ=255)
                                                                  aaaTGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTAc                       >  2:4313286/1‑93 (MQ=255)
                                                                   aaTGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTCACAGCTACCTGATTTTCGTCCTGCTGTATGTAcc                      >  2:1318089/1‑93 (MQ=255)
                                                                    aTGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCa                     <  2:581863/93‑1 (MQ=255)
                                                                      gggTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATg                   >  2:3339629/1‑93 (MQ=255)
                                                                       ggTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGt                  <  2:296260/93‑1 (MQ=255)
                                                                       ggTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGt                  <  2:4898630/93‑1 (MQ=255)
                                                                        gTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTa                 <  2:462912/93‑1 (MQ=255)
                                                                         tACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTAt                <  2:2003449/93‑1 (MQ=255)
                                                                          aCCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTAtc               <  2:3177719/93‑1 (MQ=255)
                                                                          aCCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTAtc               <  1:5174480/93‑1 (MQ=255)
                                                                           ccGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTAtct              <  2:280354/93‑1 (MQ=255)
                                                                           ccGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTAtct              >  2:1509353/1‑93 (MQ=255)
                                                                            cGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTAtctc             <  2:57533/93‑1 (MQ=255)
                                                                            cGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTAtctc             <  2:2309383/93‑1 (MQ=255)
                                                                             ggggCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCg            >  2:1973531/1‑93 (MQ=255)
                                                                              gggCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCgg           >  2:1700541/1‑93 (MQ=255)
                                                                              gggCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCgg           >  2:2618303/1‑93 (MQ=255)
                                                                              gggCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCgg           <  2:6128034/93‑1 (MQ=255)
                                                                              gggCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCgg           >  2:6502629/1‑93 (MQ=255)
                                                                               ggCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGt          <  2:4408494/93‑1 (MQ=255)
                                                                                gCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTg         <  2:3403191/93‑1 (MQ=255)
                                                                                  gATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGAt       <  2:6042938/93‑1 (MQ=255)
                                                                                   aTGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATg      <  2:3152469/93‑1 (MQ=255)
                                                                                   aTGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATg      >  1:5276160/1‑93 (MQ=255)
                                                                                   aTGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATg      >  2:3949544/1‑93 (MQ=255)
                                                                                    tGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATgg     <  2:2071972/93‑1 (MQ=255)
                                                                                    tGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATgg     <  1:1816681/93‑1 (MQ=255)
                                                                                    tGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATgg     >  1:5816685/1‑93 (MQ=255)
                                                                                     gggCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATggg    >  1:4557643/1‑93 (MQ=255)
                                                                                      ggCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATgggg   <  2:780733/93‑1 (MQ=255)
                                                                                      ggCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATgggg   <  1:2463526/93‑1 (MQ=255)
                                                                                      ggCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATgggg   >  2:5139257/1‑93 (MQ=255)
                                                                                      ggCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATCTACCATGTATCTCGGTGCTgggg   >  2:2148757/1‑93 (MQ=255)
                                                                                          |                                                                                         
GCCTGAAAGACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGTGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGG  >  NC_000913/3539910‑3540089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGAGCCTGAAAGACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGTGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTAT  >  NC_000913/3539907‑3540093
                                                                                             |                                                                                             
AGAGCCTGAAAGACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGC                                                                                               >  SRR4302536.5852177/1‑94 (MQ=60)
    CCTGAAAGACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATG                                                                                           >  SRR4302536.2757537/1‑94 (MQ=60)
    CCTGAAAGACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATG                                                                                           >  SRR4302536.2486352/1‑94 (MQ=60)
    CCTGAAAGACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATG                                                                                           <  SRR4302536.5713386/94‑1 (MQ=60)
    CCTGAAAGACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATG                                                                                           <  SRR4302536.6623613/94‑1 (MQ=60)
     CTGAAAGACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGG                                                                                          <  SRR4302536.1993513/94‑1 (MQ=60)
     CTGAAAGACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGG                                                                                          >  SRR4302536.963349/1‑94 (MQ=60)
        AAAGACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATC                                                                                       >  SRR4302536.960299/1‑94 (MQ=60)
        AAAGACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATC                                                                                       <  SRR4302536.4817979/94‑1 (MQ=60)
        AAAGACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATC                                                                                       >  SRR4302536.38368/1‑94 (MQ=60)
        AAAGACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGCGCGCGATGGATC                                                                                       >  SRR4302536.4732760/1‑94 (MQ=60)
         AAGACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCA                                                                                      >  SRR4302536.1329231/1‑94 (MQ=60)
         AAGACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCA                                                                                      >  SRR4302536.1276134/1‑94 (MQ=60)
         AAGACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCA                                                                                      >  SRR4302536.460223/1‑94 (MQ=60)
          AGACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAG                                                                                     >  SRR4302536.2201735/1‑94 (MQ=60)
           GACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGA                                                                                    <  SRR4302536.2186853/94‑1 (MQ=60)
           GACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGA                                                                                    >  SRR4302536.1589740/1‑94 (MQ=60)
           GACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGA                                                                                    <  SRR4302536.3272793/94‑1 (MQ=60)
           GACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGA                                                                                    <  SRR4302536.6393906/94‑1 (MQ=60)
            ACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCcgca                                                                                   >  SRR4302536.1868124/1‑90 (MQ=60)
             CACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAA                                                                                  >  SRR4302536.5858057/1‑94 (MQ=60)
             CACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGAGGGATCAGAAA                                                                                  >  SRR4302536.1067399/1‑94 (MQ=60)
              gcgTTCAGCATTAGCGAACGGATGAACCCCATTGGAGCCAGCAAGGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAAT                                                                                 <  SRR4302536.6897471/91‑1 (MQ=60)
              ACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAAT                                                                                 >  SRR4302536.997607/1‑94 (MQ=60)
                CTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTC                                                                               <  SRR4302536.5889655/94‑1 (MQ=60)
                 TTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGCGTACCGGCGCGATTGGCGCGCTGGGTCACAAATTCG                                                                              >  SRR4302536.5828522/1‑94 (MQ=60)
                   CAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGT                                                                            <  SRR4302536.4092968/94‑1 (MQ=60)
                    aggcttagccgcgTGATGAACCCCATTGAAGCCAACAAAAGCGACGGCAAAATGGGTTCCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTA                                                                           <  SRR4302536.2887056/81‑1 (MQ=60)
                     GCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAG                                                                          <  SRR4302536.6405973/94‑1 (MQ=60)
                     GCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAG                                                                          <  SRR4302536.3506458/94‑1 (MQ=60)
                      CCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGTAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGC                                                                         >  SRR4302536.5543782/1‑94 (MQ=60)
                       CTTAGCTTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCG                                                                        <  SRR4302536.705059/94‑1 (MQ=60)
                       CTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCG                                                                        <  SRR4302536.5869589/94‑1 (MQ=60)
                       CTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCG                                                                        >  SRR4302536.4763292/1‑94 (MQ=60)
                       CTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCG                                                                        >  SRR4302536.4411039/1‑94 (MQ=60)
                         TAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCA                                                                      >  SRR4302536.315408/1‑94 (MQ=60)
                          AGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAG                                                                     <  SRR4302536.5135411/94‑1 (MQ=60)
                           GCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGC                                                                    >  SRR4302536.336405/1‑94 (MQ=60)
                            CGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGGGCGATGGATCAGAAATTCGGTAGGGGAGCA                                                                   >  SRR4302536.2421531/1‑94 (MQ=60)
                            CCTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCA                                                                   <  SRR4302536.1731338/94‑1 (MQ=60)
                             GTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAG                                                                  >  SRR4302536.3301114/1‑94 (MQ=60)
                             GTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAG                                                                  >  SRR4302536.3825031/1‑94 (MQ=60)
                              TACTGATGAACCCCATTGAAGCCAGCAAAGGCAACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGC                                                                 <  SRR4302536.6456625/94‑1 (MQ=60)
                              TACTGATGAACCCCATCGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGC                                                                 <  SRR4302536.4049776/94‑1 (MQ=60)
                               ACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCA                                                                <  SRR4302536.3945905/94‑1 (MQ=60)
                                CTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAG                                                               >  SRR4302536.2254928/1‑94 (MQ=60)
                                CTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGAAG                                                               >  SRR4302536.2783383/1‑94 (MQ=60)
                                 TGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGC                                                              <  SRR4302536.735555/94‑1 (MQ=60)
                                  GATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCT                                                             <  SRR4302536.5592220/94‑1 (MQ=60)
                                  GATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCT                                                             <  SRR4302536.1233599/94‑1 (MQ=60)
                                  GATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCT                                                             <  SRR4302536.193907/94‑1 (MQ=60)
                                   ATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTT                                                            <  SRR4302536.118274/94‑1 (MQ=60)
                                    TGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTA                                                           >  SRR4302536.3188414/1‑94 (MQ=60)
                                    TGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTA                                                           <  SRR4302536.4319983/94‑1 (MQ=60)
                                    TGAACCCCATTGAAGCCAGCAAAGGCGACCGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTA                                                           >  SRR4302536.1833008/1‑94 (MQ=60)
                                     GAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTAC                                                          >  SRR4302536.3149925/1‑94 (MQ=60)
                                     GAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTAC                                                          >  SRR4302536.160220/1‑94 (MQ=60)
                                     GAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTAC                                                          <  SRR4302536.5214121/94‑1 (MQ=60)
                                      AACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACA                                                         >  SRR4302536.3661558/1‑94 (MQ=60)
                                      AACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACA                                                         <  SRR4302536.2888630/94‑1 (MQ=60)
                                      AACCCCATAGAAGCCAGAAAAGGCGACGGCGAAATGGGTACCGGGGCGATTGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACA                                                         <  SRR4302536.41283/94‑1 (MQ=60)
                                       ACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAG                                                        <  SRR4302536.1688453/94‑1 (MQ=60)
                                       ACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAG                                                        <  SRR4302536.4801116/94‑1 (MQ=60)
                                        CCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGC                                                       >  SRR4302536.3141631/1‑94 (MQ=60)
                                          CCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTA                                                     <  SRR4302536.5967505/94‑1 (MQ=60)
                                            ATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGATATTCGGTAGCGCAGCAGCAGCTTACATCTACC                                                   >  SRR4302536.4170138/1‑94 (MQ=60)
                                            ATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACC                                                   >  SRR4302536.106193/1‑94 (MQ=60)
                                            ATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACC                                                   <  SRR4302536.5082921/94‑1 (MQ=60)
                                            ATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACC                                                   >  SRR4302536.2961907/1‑94 (MQ=60)
                                              TGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGGAGCGTACAGCTACCTT                                                 >  SRR4302536.5254468/1‑94 (MQ=60)
                                              TGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTG                                                 >  SRR4302536.5013033/1‑94 (MQ=60)
                                              TGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTG                                                 <  SRR4302536.1127202/94‑1 (MQ=60)
                                              TGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTG                                                 <  SRR4302536.5427587/94‑1 (MQ=60)
                                              TGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTG                                                 <  SRR4302536.2199453/94‑1 (MQ=60)
                                                AAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGAT                                               >  SRR4302536.731836/1‑94 (MQ=60)
                                                AAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGAT                                               <  SRR4302536.3892120/94‑1 (MQ=60)
                                                AAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGAT                                               >  SRR4302536.5707189/1‑94 (MQ=60)
                                                AAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGAT                                               <  SRR4302536.5775337/94‑1 (MQ=60)
                                                 AGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATT                                              >  SRR4302536.1035378/1‑94 (MQ=60)
                                                 AGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATT                                              >  SRR4302536.5409455/1‑94 (MQ=60)
                                                 AGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATT                                              >  SRR4302536.5949900/1‑94 (MQ=60)
                                                  GCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTT                                             <  SRR4302536.6125758/94‑1 (MQ=60)
                                                  GCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTT                                             <  SRR4302536.4086772/94‑1 (MQ=60)
                                                  GCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTT                                             <  SRR4302536.4436882/94‑1 (MQ=60)
                                                  GCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTT                                             <  SRR4302536.6877346/94‑1 (MQ=60)
                                                  GCCAGCAAAGGCGACGGCGAAATGGGCACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTT                                             <  SRR4302536.6081899/94‑1 (MQ=60)
                                                   CCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGGAGCAGCTTACAGCTACCTGATTTT                                            >  SRR4302536.5454166/1‑94 (MQ=60)
                                                   CCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTT                                            >  SRR4302536.3957029/1‑94 (MQ=60)
                                                    CAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTC                                           <  SRR4302536.2468362/94‑1 (MQ=60)
                                                    CAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCACATTACAGCTACCTGATTTTC                                           >  SRR4302536.1580931/1‑94 (MQ=60)
                                                     AGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCG                                          >  SRR4302536.4063666/1‑94 (MQ=60)
                                                     AGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCG                                          >  SRR4302536.6656646/1‑94 (MQ=60)
                                                       CAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTC                                        >  SRR4302536.953430/1‑94 (MQ=60)
                                                        AAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCC                                       >  SRR4302536.3758974/1‑94 (MQ=60)
                                                        AAAGGAGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGAAGCTTATAGCTACCTGATTTTCGTCC                                       >  SRR4302536.2795416/1‑94 (MQ=60)
                                                         AAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGGGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCT                                      >  SRR4302536.5988360/1‑94 (MQ=60)
                                                         AAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCT                                      <  SRR4302536.179144/94‑1 (MQ=60)
                                                         AAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCT                                      <  SRR4302536.5778920/94‑1 (MQ=60)
                                                         AAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCT                                      >  SRR4302536.4545498/1‑94 (MQ=60)
                                                         AAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCT                                      >  SRR4302536.6635365/1‑94 (MQ=60)
                                                          AGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGGGATGGATCAGAAATTCGGTAGCGCAGCAGCAGATTACAGCTACCTGATTTTCGTACTG                                     >  SRR4302536.3329644/1‑94 (MQ=60)
                                                          AGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTTCTG                                     >  SRR4302536.6117748/1‑94 (MQ=60)
                                                          AGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTG                                     <  SRR4302536.116710/94‑1 (MQ=60)
                                                          AGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTG                                     >  SRR4302536.2540263/1‑94 (MQ=60)
                                                          AGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTG                                     <  SRR4302536.2446234/94‑1 (MQ=60)
                                                          AGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTG                                     <  SRR4302536.6264863/94‑1 (MQ=60)
                                                          AGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTG                                     <  SRR4302536.3301474/94‑1 (MQ=60)
                                                          AGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTG                                     >  SRR4302536.1194156/1‑94 (MQ=60)
                                                           GGCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGC                                    >  SRR4302536.3233440/1‑94 (MQ=60)
                                                            GCTACGGAGAAATGGGTACCGGGGCTATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCT                                   <  SRR4302536.2692991/94‑1 (MQ=60)
                                                            GCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTCGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCT                                   <  SRR4302536.3598540/94‑1 (MQ=60)
                                                            GCGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCT                                   <  SRR4302536.965679/94‑1 (MQ=60)
                                                             CGACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTG                                  >  SRR4302536.337071/1‑94 (MQ=60)
                                                              GACGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGT                                 <  SRR4302536.5564122/94‑1 (MQ=60)
                                                                CGGCGAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTAT                               <  SRR4302536.259425/94‑1 (MQ=60)
                                                                    GAAATGGGTACCGGGGCGCTGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGCTTTTCGTCCTGCTGTATGTAC                           >  SRR4302536.1171847/1‑94 (MQ=60)
                                                                    GAAATGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTAC                           >  SRR4302536.4781271/1‑94 (MQ=60)
                                                                        TGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATG                       <  SRR4302536.47392/94‑1 (MQ=60)
                                                                        TGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATG                       >  SRR4302536.2015565/1‑94 (MQ=60)
                                                                        AGGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATG                       <  SRR4302536.2350776/94‑1 (MQ=60)
                                                                         GGGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGT                      >  SRR4302536.3702694/1‑94 (MQ=60)
                                                                          GGTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTA                     <  SRR4302536.987382/94‑1 (MQ=60)
                                                                           GTACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTAT                    <  SRR4302536.186627/94‑1 (MQ=60)
                                                                            TACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATC                   >  SRR4302536.3482916/1‑94 (MQ=60)
                                                                             ACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCT                  <  SRR4302536.94958/94‑1 (MQ=60)
                                                                             ACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCT                  <  SRR4302536.5328858/94‑1 (MQ=60)
                                                                             ACCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCT                  >  SRR4302536.4531755/1‑94 (MQ=60)
                                                                              CCGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTC                 <  SRR4302536.3834497/94‑1 (MQ=60)
                                                                               CGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCG                <  SRR4302536.5136246/94‑1 (MQ=60)
                                                                               CGGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCG                <  SRR4302536.3201545/94‑1 (MQ=60)
                                                                                GGGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGG               <  SRR4302536.1785269/94‑1 (MQ=60)
                                                                                 GGGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGT              <  SRR4302536.20562/94‑1 (MQ=60)
                                                                                  GGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTG             >  SRR4302536.588682/1‑94 (MQ=60)
                                                                                  GGCGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTG             <  SRR4302536.662319/94‑1 (MQ=60)
                                                                                    CGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGAT           >  SRR4302536.5511696/1‑94 (MQ=60)
                                                                                    CGATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGAT           <  SRR4302536.1623531/94‑1 (MQ=60)
                                                                                     GATGGTCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATG          <  SRR4302536.6626995/94‑1 (MQ=60)
                                                                                     GATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATG          <  SRR4302536.2942903/94‑1 (MQ=60)
                                                                                     GATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATG          >  SRR4302536.5433405/1‑94 (MQ=60)
                                                                                      ATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGG         >  SRR4302536.5989953/1‑94 (MQ=60)
                                                                                      ATGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGG         >  SRR4302536.4670570/1‑94 (MQ=60)
                                                                                       TGGGCGCGATGGATCAGTAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGG        <  SRR4302536.2929316/94‑1 (MQ=60)
                                                                                       TGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGG        <  SRR4302536.1871492/94‑1 (MQ=60)
                                                                                       TGGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGG        >  SRR4302536.4694371/1‑94 (MQ=60)
                                                                                       TAGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCGGCTGTATGTACCATGTATCTGGGTGATGGG        <  SRR4302536.5935547/94‑1 (MQ=60)
                                                                                        TGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGG       >  SRR4302536.3223503/1‑94 (MQ=60)
                                                                                        GGGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGG       <  SRR4302536.6488051/94‑1 (MQ=60)
                                                                                         GGCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGG      <  SRR4302536.2537917/94‑1 (MQ=60)
                                                                                          GCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGG     >  SRR4302536.1512939/1‑94 (MQ=60)
                                                                                          GCGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGC     <  SRR4302536.5059675/94‑1 (MQ=60)
                                                                                           CGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCT    <  SRR4302536.6017973/94‑1 (MQ=60)
                                                                                           CGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCT    <  SRR4302536.5181407/94‑1 (MQ=60)
                                                                                           CGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCT    <  SRR4302536.1481097/94‑1 (MQ=60)
                                                                                           CGCGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCT    <  SRR4302536.1111893/94‑1 (MQ=60)
                                                                                             CGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTAT  >  SRR4302536.4172906/1‑94 (MQ=60)
                                                                                             |                                                                                             
AGAGCCTGAAAGACACCTTCAGCCTTAGCGTACTGATGAACCCCATTGAAGCCAGCAAAGGCGACGGCGAAATGGGTACCGGGGCGATGGGCGTGATGGATCAGAAATTCGGTAGCGCAGCAGCAGCTTACAGCTACCTGATTTTCGTCCTGCTGTATGTACCATGTATCTCGGTGATGGGGGCTAT  >  NC_000913/3539907‑3540093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑