Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,477,068 +C intergenic (‑178/‑242) b4256 ← / → yjgN hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,477,0681.C100.0% 747.7 / NA 226intergenic (‑178/‑242)b4256/yjgNhypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (55/171);  total (55/171)

Only 100 of 240 total aligned reads displayed.

GTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGCGAGGATTATCCTGCAGCGTCAGGCGACG‑ATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCT  >  NC_000913/4476976‑4477153
                                                                                             |                                                                                     
gtgtAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGcgacgc                                                                                       <  2:695097/93‑2 (MQ=255)
  gtAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGcgacgcat                                                                                     >  2:5755620/1‑90 (MQ=255)
  gtAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGcgacgcat                                                                                     >  2:3175644/1‑90 (MQ=255)
   tAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGcgacgcatt                                                                                    <  2:1808063/93‑5 (MQ=255)
   tAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGcgacgcatt                                                                                    <  1:1035625/93‑5 (MQ=255)
     gCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGGGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTAc                                                                                  >  2:3338528/1‑93 (MQ=255)
     gCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTAc                                                                                  <  2:5565802/93‑1 (MQ=255)
       cTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAg                                                                                <  1:6356401/93‑1 (MQ=255)
       cTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAg                                                                                >  2:501018/1‑93 (MQ=255)
       cTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAg                                                                                <  1:3044282/93‑1 (MQ=255)
         ttATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGt                                                                              <  1:2442616/93‑1 (MQ=255)
         ttATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGt                                                                              <  2:4618012/93‑1 (MQ=255)
          tATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTg                                                                             <  2:3143684/93‑1 (MQ=255)
          tATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTg                                                                             <  1:5343981/93‑1 (MQ=255)
            tCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGAt                                                                           <  2:3835308/93‑1 (MQ=255)
            tCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGAt                                                                           >  1:4175334/1‑93 (MQ=255)
            tCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGAt                                                                           <  2:869576/93‑1 (MQ=255)
               gCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTgc                                                                        >  2:3638282/1‑93 (MQ=255)
               gCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTgc                                                                        <  2:1203563/93‑1 (MQ=255)
                 ggTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTgcgg                                                                      >  2:1835482/1‑93 (MQ=255)
                 ggTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGGGATTgcgg                                                                      >  1:3856190/1‑93 (MQ=255)
                  gTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTgcggc                                                                     <  1:1209073/93‑1 (MQ=255)
                  gTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTgcggc                                                                     <  1:5302307/93‑1 (MQ=255)
                       aCCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAAt                                                                <  1:4055033/93‑1 (MQ=255)
                        ccGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATg                                                               <  2:5817714/93‑1 (MQ=255)
                        ccGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATg                                                               <  2:1369280/93‑1 (MQ=255)
                         cGTATTCGTCGGAGACCTGACGAATGACGCGGGCGATGGC‑AGGCGTATCCTGCAGCGTCAGGCGACGCATGACAGGTGATTGCGGCGCAATGt                                                              <  2:5442890/93‑1 (MQ=255)
                         cGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGt                                                              <  1:2328165/93‑1 (MQ=255)
                         cGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGt                                                              <  1:2846473/93‑1 (MQ=255)
                           tATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTa                                                            <  2:3733274/93‑1 (MQ=255)
                           tATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTa                                                            <  2:2855502/93‑1 (MQ=255)
                           tATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTa                                                            <  1:494732/93‑1 (MQ=255)
                              tCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTc                                                         <  1:3255196/93‑1 (MQ=255)
                                ggcggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCAt                                                       <  2:6568087/93‑1 (MQ=255)
                                 gcggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATa                                                      <  2:3124485/93‑1 (MQ=255)
                                 gcggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATa                                                      <  1:4247235/93‑1 (MQ=255)
                                   ggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGt                                                    >  1:1549295/1‑93 (MQ=255)
                                    gATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGtt                                                   >  1:5227405/1‑93 (MQ=255)
                                    gATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGtt                                                   >  2:5093037/1‑93 (MQ=255)
                                      tACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTa                                                 <  2:5219644/93‑1 (MQ=255)
                                      tACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTa                                                 >  2:1891290/1‑93 (MQ=255)
                                          tGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAAccc                                             <  1:5123400/93‑1 (MQ=255)
                                          tGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAAccc                                             <  2:4158127/93‑1 (MQ=255)
                                          tGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAAccc                                             <  2:2692812/93‑1 (MQ=255)
                                             cGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaat                                          <  1:333825/93‑1 (MQ=255)
                                             cGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaat                                          <  2:669057/93‑1 (MQ=255)
                                             cGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaat                                          <  1:5454599/93‑1 (MQ=255)
                                              gAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaata                                         <  2:3013134/93‑1 (MQ=255)
                                                aTGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaataaa                                       <  1:1649886/93‑1 (MQ=255)
                                                aTGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaataaa                                       <  1:3113838/93‑1 (MQ=255)
                                                 tGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaataaaa                                      <  2:4905681/93‑1 (MQ=255)
                                                 tGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaataaaa                                      >  1:4797548/1‑93 (MQ=255)
                                                 tGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaataaaa                                      <  1:3956457/93‑1 (MQ=255)
                                                  gACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaataaaaa                                     <  2:1016380/93‑1 (MQ=255)
                                                      cgGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaataaaaataaa                                 <  1:225480/93‑1 (MQ=255)
                                                      cgGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaataaaaataaa                                 <  1:373488/93‑1 (MQ=255)
                                                       gggCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAg                                <  2:4512332/93‑1 (MQ=255)
                                                        ggCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGt                               <  2:3215446/93‑1 (MQ=255)
                                                         gCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTa                              <  1:1943568/93‑1 (MQ=255)
                                                              tagcaGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATta                          <  1:919619/89‑1 (MQ=255)
                                                              tagcaGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATta                          <  2:6296891/89‑1 (MQ=255)
                                                              tagcaGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATta                          <  1:1460842/89‑1 (MQ=255)
                                                              tagcaGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATta                          >  1:599525/5‑93 (MQ=255)
                                                                gcaGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATtata                        <  2:338987/91‑1 (MQ=255)
                                                                gcaGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATtata                        >  1:4355166/3‑93 (MQ=255)
                                                                 caGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATAc                       <  1:1067821/92‑1 (MQ=255)
                                                                 caGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATAc                       <  1:2873044/92‑1 (MQ=255)
                                                                 caGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATAc                       <  1:5408134/92‑1 (MQ=255)
                                                                  aGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACa                      <  2:2598198/93‑1 (MQ=255)
                                                                  aGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACa                      <  2:3036303/93‑1 (MQ=255)
                                                                  aGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACa                      <  1:2940/93‑1 (MQ=255)
                                                                   ggATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACAt                     <  1:5061051/93‑1 (MQ=255)
                                                                    gATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACAtt                    <  2:5486282/93‑1 (MQ=255)
                                                                    gATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACAtt                    <  1:5718385/93‑1 (MQ=255)
                                                                     aTTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACAttt                   <  1:5572090/93‑1 (MQ=255)
                                                                      ttATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTg                  <  1:3461477/93‑1 (MQ=255)
                                                                       tATCCTGCAACGTCAGGGCACGCATTACAGGTTATTGCGGCACAATGTTAATCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGt                 <  1:6647409/93‑1 (MQ=255)
                                                                        aTCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTTACCCAATAAAAATAAAGTAATTATACATTTGtt                <  2:2543221/93‑1 (MQ=255)
                                                                         tCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGttt               <  2:4824284/93‑1 (MQ=255)
                                                                         tCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGttt               <  2:5891959/93‑1 (MQ=255)
                                                                          ccTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTa              <  2:4825370/93‑1 (MQ=255)
                                                                          ccTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTa              <  1:4531436/93‑1 (MQ=255)
                                                                          ccTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTa              <  1:5917454/93‑1 (MQ=255)
                                                                          ccTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTa              <  1:6280084/93‑1 (MQ=255)
                                                                           cTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTaa             <  2:1699725/93‑1 (MQ=255)
                                                                            tGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAAt            <  1:2519923/93‑1 (MQ=255)
                                                                            tGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAAt            <  2:4097288/93‑1 (MQ=255)
                                                                             gCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATa           <  2:2199898/93‑1 (MQ=255)
                                                                             gCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATa           <  1:6199625/93‑1 (MQ=255)
                                                                                gCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCa        >  1:4741448/1‑93 (MQ=255)
                                                                                gCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCa        <  2:5957464/93‑1 (MQ=255)
                                                                                 ggTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCAc       <  2:395749/92‑1 (MQ=255)
                                                                                 cGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCAc       <  2:5645111/93‑1 (MQ=255)
                                                                                 cGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCAc       <  2:837259/93‑1 (MQ=255)
                                                                                  gTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCAct      >  2:2089721/1‑93 (MQ=255)
                                                                                   tCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCActc     <  1:6586056/93‑1 (MQ=255)
                                                                                   tCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCActc     <  1:4473482/93‑1 (MQ=255)
                                                                                   tCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCActc     >  1:5086039/1‑93 (MQ=255)
                                                                                     aGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCActctc   >  1:2073647/1‑93 (MQ=255)
                                                                                      ggCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCActctct  >  1:3631706/1‑93 (MQ=255)
                                                                                             |                                                                                     
GTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGCGAGGATTATCCTGCAGCGTCAGGCGACG‑ATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCT  >  NC_000913/4476976‑4477153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGCGAGGATTATCCTGCAGCGTCAGGCGACG‑ATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTTA  >  NC_000913/4476977‑4477158
                                                                                           |                                                                                           
TGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGcat                                                                                          <  SRR4302536.3240824/94‑4 (MQ=60)
  TAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTA                                                                                        <  SRR4302536.1066354/94‑1 (MQ=60)
  TAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTA                                                                                        <  SRR4302536.2072746/94‑1 (MQ=60)
    GCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACA                                                                                      <  SRR4302536.296367/94‑1 (MQ=60)
     CCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAG                                                                                     <  SRR4302536.4382703/94‑1 (MQ=60)
      CTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGG                                                                                    <  SRR4302536.6545768/94‑1 (MQ=60)
      CTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGG                                                                                    <  SRR4302536.3133756/94‑1 (MQ=60)
       TTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGT                                                                                   >  SRR4302536.1177139/1‑94 (MQ=60)
       TTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGT                                                                                   >  SRR4302536.5636195/1‑94 (MQ=60)
       TTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGT                                                                                   <  SRR4302536.3610844/94‑1 (MQ=60)
        TTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTG                                                                                  <  SRR4302536.5936867/94‑1 (MQ=60)
        TTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTG                                                                                  <  SRR4302536.2516436/94‑1 (MQ=60)
         TATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGA                                                                                 <  SRR4302536.5503576/94‑1 (MQ=60)
         TATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGA                                                                                 <  SRR4302536.37245/94‑1 (MQ=60)
          ATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGAT                                                                                >  SRR4302536.4299374/1‑94 (MQ=60)
          ATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCGGCAGCGTCAGGCGACGCATTACAGGTGAT                                                                                >  SRR4302536.6796528/1‑94 (MQ=60)
           TCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATT                                                                               >  SRR4302536.4504190/1‑94 (MQ=60)
           TCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATT                                                                               <  SRR4302536.6485940/94‑1 (MQ=60)
            CAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTG                                                                              <  SRR4302536.6214554/94‑1 (MQ=60)
              GCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCG                                                                            <  SRR4302536.5098642/94‑1 (MQ=60)
              GCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCG                                                                            <  SRR4302536.2949189/94‑1 (MQ=60)
              GCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCG                                                                            <  SRR4302536.3524326/94‑1 (MQ=60)
               CGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGGGATTGCGG                                                                           >  SRR4302536.3970956/1‑94 (MQ=60)
               CGGGAAGACCGTATTCGGCGGATACCTGACGAATGCCGCGGGCGATAGC‑AGGATTATCCTGCCGCGTCAGGCGACGCATTACAGGTGATTGCGG                                                                           >  SRR4302536.5175229/1‑94 (MQ=60)
                GGTAAGACCGTATTCGGCGGCTACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGC                                                                          >  SRR4302536.6741476/1‑94 (MQ=60)
                GGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGC                                                                          <  SRR4302536.1540903/94‑1 (MQ=60)
                 GTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCG                                                                         <  SRR4302536.1245407/94‑1 (MQ=60)
                 GTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCG                                                                         <  SRR4302536.5460473/94‑1 (MQ=60)
                     GACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAAT                                                                     >  SRR4302536.1076606/1‑94 (MQ=60)
                     GACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAAT                                                                     <  SRR4302536.4287794/94‑1 (MQ=60)
                      ACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATG                                                                    <  SRR4302536.4371745/94‑1 (MQ=60)
                      ACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATG                                                                    <  SRR4302536.2939056/94‑1 (MQ=60)
                      ACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATG                                                                    <  SRR4302536.4175240/94‑1 (MQ=60)
                      ACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTACCCTGCAGCGTCAGGCGACGCATTACAGGTGATTTCGGCGCAATG                                                                    <  SRR4302536.3793283/94‑1 (MQ=60)
                       CCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGT                                                                   <  SRR4302536.4623756/94‑1 (MQ=60)
                       CCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGT                                                                   <  SRR4302536.495909/94‑1 (MQ=60)
                        CGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTT                                                                  <  SRR4302536.5220189/94‑1 (MQ=60)
                        CGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTT                                                                  <  SRR4302536.2398818/94‑1 (MQ=60)
                        CGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTT                                                                  <  SRR4302536.2930652/94‑1 (MQ=60)
                        CGTATTCGGCCGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTT                                                                  <  SRR4302536.2287446/94‑1 (MQ=60)
                         GTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTA                                                                 >  SRR4302536.5468971/1‑94 (MQ=60)
                          TATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTAT                                                                <  SRR4302536.507511/94‑1 (MQ=60)
                             TCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCA                                                             <  SRR4302536.3350627/94‑1 (MQ=60)
                             TCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCA                                                             <  SRR4302536.552162/94‑1 (MQ=60)
                              CGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCAT                                                            <  SRR4302536.6300760/94‑1 (MQ=60)
                              CGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCAT                                                            >  SRR4302536.2591760/1‑94 (MQ=60)
                                GCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAG                                                          <  SRR4302536.4373747/94‑1 (MQ=60)
                                 CGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGT                                                         >  SRR4302536.1594832/1‑94 (MQ=60)
                                  GGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTT                                                        >  SRR4302536.5383159/1‑94 (MQ=60)
                                    ATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTA                                                      >  SRR4302536.5752863/1‑94 (MQ=60)
                                    ATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTA                                                      <  SRR4302536.2515729/94‑1 (MQ=60)
                                      ACCTGGCGAATGAAGCGGACTATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAAC                                                    <  SRR4302536.1245513/94‑1 (MQ=60)
                                      ACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAAC                                                    <  SRR4302536.5604046/94‑1 (MQ=60)
                                        CTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCC                                                  >  SRR4302536.5495474/1‑94 (MQ=60)
                                         TGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCA                                                 <  SRR4302536.2740622/94‑1 (MQ=60)
                                         TGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCA                                                 <  SRR4302536.5276475/94‑1 (MQ=60)
                                          GACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAA                                                <  SRR4302536.3081703/94‑1 (MQ=60)
                                           ACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAAT                                               <  SRR4302536.4805720/94‑1 (MQ=60)
                                            CGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATA                                              <  SRR4302536.5617721/94‑1 (MQ=60)
                                            CGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATA                                              >  SRR4302536.5902689/1‑94 (MQ=60)
                                            CGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATA                                              <  SRR4302536.342684/94‑1 (MQ=60)
                                              AATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAA                                            >  SRR4302536.831745/1‑94 (MQ=60)
                                               ATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAA                                           <  SRR4302536.405965/94‑1 (MQ=60)
                                               ATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAA                                           <  SRR4302536.3204775/94‑1 (MQ=60)
                                               ATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAA                                           <  SRR4302536.1697839/94‑1 (MQ=60)
                                               ATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAA                                           >  SRR4302536.4941949/1‑94 (MQ=60)
                                                TGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAA                                          >  SRR4302536.5242081/1‑94 (MQ=60)
                                                TGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAA                                          <  SRR4302536.4073940/94‑1 (MQ=60)
                                                TGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAA                                          >  SRR4302536.2446324/1‑94 (MQ=60)
                                                   CGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAA                                       <  SRR4302536.3073374/94‑1 (MQ=60)
                                                     CGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAG                                     <  SRR4302536.86553/94‑1 (MQ=60)
                                                     CGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAG                                     <  SRR4302536.231476/94‑1 (MQ=60)
                                                     CGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAG                                     <  SRR4302536.383339/94‑1 (MQ=60)
                                                     CGGGCGATAGC‑AGCATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCTCAATGTTATTCATAGTTTAACCCAATAAAAATAAAG                                     <  SRR4302536.1646242/94‑1 (MQ=60)
                                                      GGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGT                                    <  SRR4302536.3911486/94‑1 (MQ=60)
                                                      GGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGT                                    >  SRR4302536.4930425/1‑94 (MQ=60)
                                                      GGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGA                                    >  SRR4302536.42391/1‑94 (MQ=60)
                                                       GGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTA                                   <  SRR4302536.4714707/94‑1 (MQ=60)
                                                       GGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTA                                   <  SRR4302536.6118087/94‑1 (MQ=60)
                                                       GGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTA                                   <  SRR4302536.2380087/94‑1 (MQ=60)
                                                       GGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTA                                   <  SRR4302536.1708234/94‑1 (MQ=60)
                                                       GGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCAGTACAGGTGATTGCGGCGAAATGTTATTCATAGTTTATCCCAACACAAATAAAGTA                                   <  SRR4302536.1020244/94‑1 (MQ=60)
                                                        GCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAA                                  <  SRR4302536.2002008/94‑1 (MQ=60)
                                                         CGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAAT                                 >  SRR4302536.6656717/1‑94 (MQ=60)
                                                          GATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATT                                >  SRR4302536.6450395/1‑94 (MQ=60)
                                                          GATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATT                                <  SRR4302536.4011965/94‑1 (MQ=60)
                                                           ATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTA                               >  SRR4302536.616717/1‑94 (MQ=60)
                                                           ATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTA                               <  SRR4302536.815743/94‑1 (MQ=60)
                                                            TAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTAT                              <  SRR4302536.946719/94‑1 (MQ=60)
                                                            TAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTAT                              <  SRR4302536.1504061/94‑1 (MQ=60)
                                                             AGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATA                             >  SRR4302536.4485432/1‑94 (MQ=60)
                                                                CAGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACA                           <  SRR4302536.2290530/94‑1 (MQ=60)
                                                                CAGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACA                           <  SRR4302536.2957873/94‑1 (MQ=60)
                                                                CAGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACA                           <  SRR4302536.1099743/94‑1 (MQ=60)
                                                                CAGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACA                           <  SRR4302536.5569736/94‑1 (MQ=60)
                                                                 AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACAT                          >  SRR4302536.5260111/1‑94 (MQ=60)
                                                                 AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACAT                          <  SRR4302536.3020/94‑1 (MQ=60)
                                                                  GGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATT                         <  SRR4302536.5212643/94‑1 (MQ=60)
                                                                   GATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTT                        <  SRR4302536.3390301/94‑1 (MQ=60)
                                                                   GATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTT                        <  SRR4302536.5320726/94‑1 (MQ=60)
                                                                   GATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTT                        <  SRR4302536.5888434/94‑1 (MQ=60)
                                                                    ATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTG                       <  SRR4302536.5737973/94‑1 (MQ=60)
                                                                     TTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGT                      >  SRR4302536.1641480/1‑94 (MQ=60)
                                                                     TTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGT                      <  SRR4302536.3564624/94‑1 (MQ=60)
                                                                      TATCCTGCAACGTCAGGGCACGCATTACAGGTTATTGCGGCACAATGTTAATCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTT                     <  SRR4302536.6845046/94‑1 (MQ=60)
                                                                       ATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTT                    <  SRR4302536.3359524/94‑1 (MQ=60)
                                                                         CCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAA                  <  SRR4302536.4667272/94‑1 (MQ=60)
                                                                         CCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAA                  <  SRR4302536.6467231/94‑1 (MQ=60)
                                                                         CCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAA                  <  SRR4302536.6093720/94‑1 (MQ=60)
                                                                          CTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAAT                 <  SRR4302536.3187602/94‑1 (MQ=60)
                                                                           TGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATA                <  SRR4302536.2595891/94‑1 (MQ=60)
                                                                            GCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATAC               >  SRR4302536.5126069/1‑94 (MQ=60)
                                                                            GCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATAC               <  SRR4302536.6384285/94‑1 (MQ=60)
                                                                            GCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATAC               <  SRR4302536.556285/94‑1 (MQ=60)
                                                                             CAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACC              >  SRR4302536.4559805/1‑94 (MQ=60)
                                                                              AGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCA             >  SRR4302536.4884118/1‑94 (MQ=60)
                                                                                CGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACT           <  SRR4302536.6429768/94‑1 (MQ=60)
                                                                                 GTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTC          <  SRR4302536.4127462/94‑1 (MQ=60)
                                                                                 GTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTC          >  SRR4302536.5238203/1‑94 (MQ=60)
                                                                                  TCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCT         <  SRR4302536.6781996/94‑1 (MQ=60)
                                                                                  TCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCT         <  SRR4302536.4607865/94‑1 (MQ=60)
                                                                                  TCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCT         <  SRR4302536.5415054/94‑1 (MQ=60)
                                                                                   CAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTC        >  SRR4302536.2136100/1‑94 (MQ=60)
                                                                                    AGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCT       >  SRR4302536.3740337/1‑94 (MQ=60)
                                                                                     GGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTT      <  SRR4302536.1268757/94‑1 (MQ=60)
                                                                                       CGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCT    <  SRR4302536.5169957/94‑1 (MQ=60)
                                                                                       CGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCT    <  SRR4302536.1693918/94‑1 (MQ=60)
                                                                                        GACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTT   <  SRR4302536.3717491/94‑1 (MQ=60)
                                                                                        GACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTT   <  SRR4302536.4055322/94‑1 (MQ=60)
                                                                                         ACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTTA  <  SRR4302536.3300382/94‑1 (MQ=60)
                                                                                           |                                                                                           
TGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGCGAGGATTATCCTGCAGCGTCAGGCGACG‑ATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTTA  >  NC_000913/4476977‑4477158

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑