Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,130,334 A→G V30V (GTT→GTC)  b2983 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,130,3340AG99.7% 937.9 / ‑5.2 286V30V (GTT→GTC) b2983hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (130/155);  total (131/155)
Fisher's exact test for biased strand distribution p-value = 4.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.34e-01

Only 100 of 327 total aligned reads displayed.

TACGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCC‑AACAGACCCAGCAAGGCGCTCACAACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAA  >  NC_000913/3130242‑3130423
                                                                                             |                                                                                         
  cGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCC‑AACAGAACCAGCAAGGCGCTCacgac                                                                                         >  1:3764906/1‑93 (MQ=255)
   gATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCC‑AACAGACCCAGCAAGGCGCTCacgaca                                                                                        <  2:2193824/93‑1 (MQ=255)
   gATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCC‑AACAGACCCACCAAGGCGCTCacgaca                                                                                        <  2:6708500/93‑1 (MQ=255)
   gATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCCTTAACGGCACACGAGAGCGCC‑CACAGACCCAGCCAAGCGCTCacgaca                                                                                        >  2:816391/1‑93 (MQ=255)
    aTTACACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCC‑AACAGACCCAGCAAGGCGCTCACGACAt                                                                                       <  2:3306361/93‑1 (MQ=255)
     ttGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCC‑AACAGACCCAGCAAGGCGCTCACGACAtt                                                                                      >  1:6228102/1‑93 (MQ=255)
     ttGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCC‑AACAGACCCAGCAAGGCGCTCACGACAtt                                                                                      <  2:6972399/93‑1 (MQ=255)
     ttGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCC‑AACAGACCCAGCAAGGCGCTCACGACAta                                                                                      <  1:4480031/93‑2 (MQ=255)
         acaATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTCCCGGCACACGAGAGCGCC‑AACAGACCCAGCAAGGCGCTCACGACATTACTg                                                                                  <  1:9393224/93‑1 (MQ=255)
          caATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCC‑AACAGACCCAGCAAGGCGCTCACGACATTACTgg                                                                                 >  2:6848306/1‑93 (MQ=255)
            aTCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCC‑AACAGACCCAGCAAGGCGCTCACGACATTACTGGAg                                                                               >  2:5905083/1‑93 (MQ=255)
              cACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCC‑AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGcc                                                                             >  1:7478614/1‑93 (MQ=255)
                  aGTACGCCAACCATGGCTGCCGTCATCCCTTTACCGGCACACGAGAGCGCC‑AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAAc                                                                         <  2:1195473/93‑1 (MQ=255)
                  aGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCC‑AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAAc                                                                         <  1:1024760/93‑1 (MQ=255)
                  aGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCC‑AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAAc                                                                         >  1:7952170/1‑93 (MQ=255)
                   gTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCC‑AACAGACCCAGCAAGGCGCTCACGACCTTACTGGAGCCTAAcc                                                                        >  1:9184359/1‑93 (MQ=255)
                    tACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCC‑AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCa                                                                       >  2:9650506/1‑93 (MQ=255)
                     acaccAAACATGGCGGGCGTCATCCCTTTCCCGGCACAGGACAGCCCA‑AACCGCCCCAGAAAGGCCCGCACGACATTACTGGAGCCTAACCAg                                                                      <  2:7567092/90‑1 (MQ=255)
                     aCGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCC‑AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAg                                                                      <  2:8775682/93‑1 (MQ=255)
                       gCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCC‑AACAGCCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGgc                                                                    <  2:3357657/93‑1 (MQ=255)
                         cAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCC‑AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGgcgc                                                                  >  1:9555684/1‑93 (MQ=255)
                          aaaCATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCC‑AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCt                                                                 >  2:3695495/1‑93 (MQ=255)
                            aCATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCC‑AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTAt                                                               <  1:6000465/93‑1 (MQ=255)
                             cATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCC‑AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTAtt                                                              <  2:1054810/93‑1 (MQ=255)
                              aTGGCGGGCGTCCTCCCCTTACCGGCACACGAGCGCGCC‑AACAGAACCAGCAAGGCGCTCACGCCATTACTGGAGCCTAACAAGGCGCTATTg                                                             >  2:7112332/1‑93 (MQ=255)
                               tGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCC‑AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGc                                                            <  1:3590792/93‑1 (MQ=255)
                                 gcgggcgTCATCCCTTTACCGGCACACGAGAGCGCC‑AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGa                                                          <  2:1434121/93‑1 (MQ=255)
                                    ggcgTCATCCCTTTACCGGCACACGAGAGCGCC‑AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGaata                                                       >  2:2484787/1‑93 (MQ=255)
                                     gcgTCATCCCTTTACCGGCACACGAGAGCGCC‑AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGaataa                                                      <  2:1706269/93‑1 (MQ=255)
                                     gcgTCATCCCTTTACCGGCACACGAGAGCGCC‑AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCCCTATTGCGaataa                                                      <  1:6721026/93‑1 (MQ=255)
                                      cttCATCCCTTTACCGGCACACGAGAGCGCC‑AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGaataat                                                     <  2:8095837/91‑1 (MQ=255)
                                      cgTCATCCCTTTCCCGGCAGACGAGAGCTCC‑AACAGACCCAGCAAGGGGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGaataat                                                     <  2:2659168/93‑1 (MQ=255)
                                      cgTCATCCCTTTACCGGCACACGAGAGCGCC‑AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGaataat                                                     <  2:4957076/93‑1 (MQ=255)
                                      cgTCATCCCTTTACCGGCACACGAGAGCGCC‑AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGaataat                                                     <  1:3027567/93‑1 (MQ=255)
                                       gTCATCCCTTTACCGGCACACGAGAGCGCC‑AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATCATg                                                    >  1:2408600/1‑93 (MQ=255)
                                       gTCATCCCTTTACCGGCACACGAGAGCGCC‑AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATg                                                    <  1:3422486/93‑1 (MQ=255)
                                       gTCATCCCTTTACCGGCACACGAGAGCGCC‑AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATg                                                    <  1:1539176/93‑1 (MQ=255)
                                       cccttCCTTTTCCCGGCACAGAAGACCCAA‑AACAGCCCAAGCAGGGCGCCCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATg                                                    <  1:3858089/89‑1 (MQ=255)
                                        tCATCCCTTTCCCGGCACACGAGAGCGCC‑AACAGACCCAGCAGGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATgg                                                   <  1:8834870/93‑1 (MQ=255)
                                        tCATCCCTTTACCGGCACACGAGAGCGCC‑AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATgg                                                   >  2:5468465/1‑93 (MQ=255)
                                         cATCCCTTTACCGGCACACGAGAGCGCC‑AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGt                                                  >  2:9329750/1‑93 (MQ=255)
                                         cATCCCTTTACCGGCACACGAGAGCGCC‑AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGt                                                  <  2:9903128/93‑1 (MQ=255)
                                           tCCCTTTACCGGCACACGAGAGCGCC‑AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGc                                                >  2:4050232/1‑93 (MQ=255)
                                           tCCCTTTACCGGCACACGAGAGCGCC‑AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGc                                                >  1:5704027/1‑93 (MQ=255)
                                           ccccTTTCCCGGCCCACG‑GAGCCCCAAACAGCCCCAGCAAGGCCCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGc                                                <  2:7758347/92‑1 (MQ=255)
                                            cccTTTACCGGCACACGAGAGCGCC‑AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCg                                               >  2:1762832/1‑93 (MQ=255)
                                             ccTTTACCGGCACACGAGAGCGCC‑AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGa                                              >  1:9703231/1‑93 (MQ=255)
                                             ccTTTACCGGCACACGAGAGCGCC‑AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGa                                              >  2:9741636/1‑93 (MQ=255)
                                               tttACCGGCACACGAGAGCGCC‑AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGaaa                                            >  1:3731005/1‑93 (MQ=255)
                                                ttACCGGCACACGAGAGCGCC‑AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAAc                                           >  2:1364304/1‑93 (MQ=255)
                                                  aCCGGCACACGAGAGCGCC‑AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAAcgc                                         <  2:9528886/93‑1 (MQ=255)
                                                    cGGCACACGAGAGCGCC‑AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACgcgc                                       <  2:6201966/93‑1 (MQ=255)
                                                     ggCACACGAGAGCGCC‑AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGcc                                      >  1:8401398/1‑93 (MQ=255)
                                                     ggCACACGAGAGCGCC‑AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGcc                                      <  2:7896307/93‑1 (MQ=255)
                                                       cacaCGAGAGCGCC‑AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCAt                                    >  2:6504409/1‑93 (MQ=255)
                                                         cacGAGAGCGCC‑AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCt                                  >  2:7500083/1‑93 (MQ=255)
                                                           cGAGAGCGCC‑AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGc                                >  1:8224164/1‑93 (MQ=255)
                                                            gagaGCGCC‑AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCa                               >  1:1092182/1‑93 (MQ=255)
                                                             agagCGCC‑AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCaa                              >  2:8721848/1‑93 (MQ=255)
                                                             agagCGCC‑AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCaa                              >  2:5175830/1‑93 (MQ=255)
                                                             agagCGCC‑AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCaa                              <  1:3501828/93‑1 (MQ=255)
                                                              gagCGCC‑AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCaaa                             >  2:2823975/1‑93 (MQ=255)
                                                              gagCGCC‑AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCaaa                             >  1:1984056/1‑93 (MQ=255)
                                                                 cgcC‑AACAGCCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCa                          <  2:553882/93‑1 (MQ=255)
                                                                  gcC‑AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCaa                         <  1:7717340/93‑1 (MQ=255)
                                                                   cc‑AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAAt                        <  2:3099589/93‑1 (MQ=255)
                                                                   cc‑AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAAt                        <  1:7836834/93‑1 (MQ=255)
                                                                   cc‑AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAAt                        >  1:7138388/1‑93 (MQ=255)
                                                                    c‑AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATg                       <  2:7042300/93‑1 (MQ=255)
                                                                       aCAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGtt                     <  2:8503220/93‑1 (MQ=255)
                                                                        cAGCCCCAGCAAGCCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGttt                    <  2:434530/93‑1 (MQ=255)
                                                                        cAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGttt                    <  1:1192511/93‑1 (MQ=255)
                                                                        cAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGttt                    <  1:1715265/93‑1 (MQ=255)
                                                                         agccccAGCACGGCGCCCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTg                   <  2:5787737/90‑1 (MQ=255)
                                                                         aGACCCAGCAAGTCCCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTg                   <  1:2837601/93‑1 (MQ=255)
                                                                         aGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTg                   >  2:3461957/1‑93 (MQ=255)
                                                                          gACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGa                  >  1:6983387/1‑93 (MQ=255)
                                                                          gACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGa                  >  2:8238796/1‑93 (MQ=255)
                                                                          agcccGGCAAGGCCCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGa                  <  1:2155416/91‑1 (MQ=255)
                                                                                gCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTaa            >  1:8671337/1‑93 (MQ=255)
                                                                                gCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTaa            <  2:8796973/93‑1 (MQ=255)
                                                                                 cgaGGCCCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTaaa           <  2:1896739/91‑1 (MQ=255)
                                                                                 cAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTaaa           >  1:9524588/1‑93 (MQ=255)
                                                                                 cAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTaaa           >  1:5307313/1‑93 (MQ=255)
                                                                                 cAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTaaa           <  1:4921196/93‑1 (MQ=255)
                                                                                 cAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTaaa           >  1:2963029/1‑93 (MQ=255)
                                                                                 cAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTaaa           <  2:6402208/93‑1 (MQ=255)
                                                                                 aaaGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTaaa           <  1:4092408/92‑1 (MQ=255)
                                                                                  aaGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTaaaa          >  2:4819734/1‑93 (MQ=255)
                                                                                  aaGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTaaaa          >  1:4588519/1‑93 (MQ=255)
                                                                                   agccgcTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAAc         <  2:7281087/90‑1 (MQ=255)
                                                                                   aGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAAc         >  2:6109468/1‑93 (MQ=255)
                                                                                    ggCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAAcc        <  1:2240145/93‑1 (MQ=255)
                                                                                    ggCGCCCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAAcc        <  1:5778673/93‑1 (MQ=255)
                                                                                      cgcTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCg      <  1:5922228/93‑1 (MQ=255)
                                                                                        cTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGcc    >  2:4752922/1‑93 (MQ=255)
                                                                                        cTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGcc    >  2:9061272/1‑93 (MQ=255)
                                                                                        cTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGcc    <  2:943236/93‑1 (MQ=255)
                                                                                         tCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCa   >  1:4720627/1‑93 (MQ=255)
                                                                                         tCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCa   >  2:6308688/1‑93 (MQ=255)
                                                                                             |                                                                                         
TACGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCC‑AACAGACCCAGCAAGGCGCTCACAACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAA  >  NC_000913/3130242‑3130423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTACGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACAACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAATTCA  >  NC_000913/3130241‑3130427
                                                                                             |                                                                                             
GTACGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACG                                                                                               <  SRR4302540.1433756/94‑1 (MQ=60)
GTACGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACG                                                                                               >  SRR4302540.3295900/1‑94 (MQ=60)
GTACGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACG                                                                                               <  SRR4302540.1107939/94‑1 (MQ=60)
GTACGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAACAAGGGGCTCACG                                                                                               >  SRR4302540.5019859/1‑94 (MQ=60)
  ACGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGAACCAGCAAGGCGCTCACGAC                                                                                             >  SRR4302540.3805983/1‑94 (MQ=60)
   CGATTGCACAATCACCAGTACGCCAACCATGGCGGGCGTCATCCCTTTCCCGGCACACGAGAGCGCCAACAGCCCCAGCAAGGCGCTCACGACA                                                                                            <  SRR4302540.5690188/94‑1 (MQ=60)
   CGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACA                                                                                            >  SRR4302540.7099638/1‑94 (MQ=60)
    GATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACAT                                                                                           >  SRR4302540.9246343/1‑94 (MQ=60)
     ATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATT                                                                                          >  SRR4302540.6297368/1‑94 (MQ=60)
     ATTGCACAATCACAAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATT                                                                                          <  SRR4302540.4617237/94‑1 (MQ=60)
      TTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATAA                                                                                         <  SRR4302540.4528615/94‑1 (MQ=60)
      TTCCACAATCCCCAGTACGCCAAACATGGCGGGCGTCATCCCTTTCCCGGCACACGAGAGCGCCAACAGCCCCAGCAAGGCGCTCACGACATTA                                                                                         <  SRR4302540.7927803/94‑1 (MQ=60)
       TGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTAC                                                                                        >  SRR4302540.3904435/1‑94 (MQ=60)
       TCCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCCCCAACAGCCCCAGCAAGGCGCTCACGACATTAC                                                                                        <  SRR4302540.5749529/94‑1 (MQ=60)
        GCACAATCCCCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACT                                                                                       <  SRR4302540.6910131/94‑1 (MQ=60)
         CACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTG                                                                                      <  SRR4302540.5830515/94‑1 (MQ=60)
          ACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTCCCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGG                                                                                     <  SRR4302540.9505001/94‑1 (MQ=60)
           aaagCACCAGTACGCCAAACATGGCGGGCTTCATCCCTTTCCCGGCACGCGAGAGCCCCAACAGCCCCAGCAAGGCGCTCACGACATTACTGGA                                                                                    <  SRR4302540.6226277/90‑1 (MQ=60)
           CAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGTCCAAAGACCCAGCAACGCGCTCACGACATTACCGGA                                                                                    >  SRR4302540.1414504/1‑94 (MQ=60)
           CAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGA                                                                                    <  SRR4302540.8579597/94‑1 (MQ=60)
           AAATCACCAGTACGCAAAACAGGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGCCCCAGCAAGGCGCTCACGACATTACTGGA                                                                                    <  SRR4302540.5885476/94‑1 (MQ=60)
            AATCACCACGACGCCAAAAATGGCGGGCGTCACCCCTTTACCGGCACACGAGAGCGCCACCAGACCCAGCAGGGCGCTCACGACATTACTGGAG                                                                                   <  SRR4302540.7912299/94‑1 (MQ=60)
             ATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGC                                                                                  <  SRR4302540.600414/94‑1 (MQ=60)
              TCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCC                                                                                 >  SRR4302540.7562079/1‑94 (MQ=60)
              TCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCC                                                                                 >  SRR4302540.4266444/1‑94 (MQ=60)
               CACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCT                                                                                <  SRR4302540.7060005/94‑1 (MQ=60)
               CACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAAGCGCTCACGACATTACTGGAGCCT                                                                                >  SRR4302540.5869917/1‑94 (MQ=60)
                 CCAGTCCGCAAAACAGGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGCCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAA                                                                              <  SRR4302540.7988946/94‑1 (MQ=60)
                 CCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAA                                                                              <  SRR4302540.6860834/94‑1 (MQ=60)
                  caggcCGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGCCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAAC                                                                             <  SRR4302540.9335945/89‑1 (MQ=60)
                  CAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAAC                                                                             >  SRR4302540.8044865/1‑94 (MQ=60)
                   ATTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACC                                                                            >  SRR4302540.8960143/1‑94 (MQ=60)
                   AGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACCTTACTGGAGCCTAACC                                                                            >  SRR4302540.9294506/1‑94 (MQ=60)
                   AGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACC                                                                            <  SRR4302540.1035155/94‑1 (MQ=60)
                   AGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACC                                                                            <  SRR4302540.1035156/94‑1 (MQ=60)
                   AGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACC                                                                            <  SRR4302540.3011734/94‑1 (MQ=60)
                   AGTACGCCAAACATGGCGGGCGTCATCCCCTTAACCGCACACGAGAGCACCCCCAGACCCCGCACGGGGCTCACAACATTACcagggcctcaca                                                                            >  SRR4302540.8696430/1‑82 (MQ=32)
                   AGTACGCAAAACATGGCGGGCGTCATCCTTTTACCGGCACACGAGAGCGCCAACAGCCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACC                                                                            <  SRR4302540.9592226/94‑1 (MQ=60)
                    GTCCGCCAAACATGGCGCGCGTCATCCCTTGACCGGCACACGAGAGCGCGAACAGACCCAGCAAGGCCCTCACGACATTACTGGAGCCTAACCA                                                                           <  SRR4302540.8520003/94‑1 (MQ=60)
                    GTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCA                                                                           <  SRR4302540.3431784/94‑1 (MQ=60)
                    GTACGCCAAACAGGGGGGGCGTCATCCCTTTCCCGGCCCACGAGAGCGCCAACAGCCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCA                                                                           <  SRR4302540.2350846/94‑1 (MQ=60)
                      ACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCCGG                                                                         >  SRR4302540.8234860/1‑94 (MQ=60)
                       CGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGC                                                                        <  SRR4302540.1557848/94‑1 (MQ=60)
                       CGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGC                                                                        <  SRR4302540.7740415/94‑1 (MQ=60)
                       CGCAAAGCATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGC                                                                        <  SRR4302540.9585621/94‑1 (MQ=60)
                         CCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGC                                                                      >  SRR4302540.1168757/1‑94 (MQ=60)
                         CCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGC                                                                      >  SRR4302540.9668689/1‑94 (MQ=60)
                           AAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTA                                                                    >  SRR4302540.3506235/1‑94 (MQ=60)
                             ACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATT                                                                  <  SRR4302540.6066969/94‑1 (MQ=60)
                             ACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATT                                                                  >  SRR4302540.1579713/1‑94 (MQ=60)
                             ACACGGCGGACGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGCCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATT                                                                  <  SRR4302540.4711141/94‑1 (MQ=60)
                              CATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCCGGCGCTATTG                                                                 >  SRR4302540.1698821/1‑94 (MQ=60)
                               ATGCCGGGCGCCATCCCTTTACCGCAACACGAGAGCGCACACAGGCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGC                                                                <  SRR4302540.6055498/94‑1 (MQ=60)
                                TGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCG                                                               <  SRR4302540.7135171/94‑1 (MQ=60)
                                TGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCG                                                               <  SRR4302540.3630016/94‑1 (MQ=60)
                                  GCGGGCGTCATCCCTTTCCCGGCACACGAGAGCGCCAACAGCCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAA                                                             <  SRR4302540.9393380/94‑1 (MQ=60)
                                   CGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAAT                                                            >  SRR4302540.4407443/1‑94 (MQ=60)
                                    GGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATA                                                           <  SRR4302540.1278323/94‑1 (MQ=60)
                                     gcggtctcccctatccggccacagaggaccgccaacagcccagGCAAGCCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAA                                                          <  SRR4302540.5909500/51‑1 (MQ=60)
                                     gcccccccccctcccCCGGCACACGACAGCCCCACCAGCCCCAGCGAGGCCCCCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAA                                                          <  SRR4302540.1899606/79‑1 (MQ=37)
                                     GGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAA                                                          >  SRR4302540.3761117/1‑94 (MQ=60)
                                      gcgcctcccttgtcCCGGCACACGAGGGCGCCAACAGACCCAGCACGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAAT                                                         <  SRR4302540.4631893/80‑1 (MQ=60)
                                      GCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCCCTATTGCGAATAAT                                                         <  SRR4302540.6795203/94‑1 (MQ=60)
                                      GCGTCATCCCTTTACCGGCACACGAGAGCGCAAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAAT                                                         <  SRR4302540.7899816/94‑1 (MQ=60)
                                       CGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATCATG                                                        >  SRR4302540.2434571/1‑94 (MQ=60)
                                       CGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATG                                                        <  SRR4302540.6643397/94‑1 (MQ=60)
                                       CGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATG                                                        <  SRR4302540.3060820/94‑1 (MQ=60)
                                       CGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATG                                                        >  SRR4302540.5890550/1‑94 (MQ=60)
                                        GTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGG                                                       <  SRR4302540.1556195/94‑1 (MQ=60)
                                        GTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGG                                                       <  SRR4302540.3459699/94‑1 (MQ=60)
                                         TCATCCCTTTCCCGGCACACGAGAGCGCCAACAGACCCAGCAGGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGT                                                      <  SRR4302540.8937058/94‑1 (MQ=60)
                                          CATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTG                                                     >  SRR4302540.5646187/1‑94 (MQ=60)
                                          CATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCACCAAGGCGCTCCCGACCTTACTGGCGCCTAACCACGCGCTATTGCGAATAATTGTG                                                     >  SRR4302540.9476674/1‑94 (MQ=60)
                                           ATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGC                                                    >  SRR4302540.5766829/1‑94 (MQ=60)
                                           ATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGC                                                    >  SRR4302540.1872369/1‑94 (MQ=60)
                                           ATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGC                                                    >  SRR4302540.3813365/1‑94 (MQ=60)
                                            ccccctccCCGGCACAGGAGAGCCCCAACAGCCCCAGAAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCG                                                   <  SRR4302540.2286648/86‑1 (MQ=60)
                                             CCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGA                                                  >  SRR4302540.2133759/1‑94 (MQ=60)
                                             CCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGA                                                  >  SRR4302540.9817295/1‑94 (MQ=60)
                                              CCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAA                                                 <  SRR4302540.3488810/94‑1 (MQ=60)
                                               cttgcccgtCACACGAGAGCGCCAACAGACCCAGCAAGGCGTTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAA                                                <  SRR4302540.2293743/85‑1 (MQ=60)
                                               cttgcccgcCACACGAGAGCGCAAACAGCCCCAGCAGGCCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAA                                                <  SRR4302540.2313269/85‑1 (MQ=60)
                                               CTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAA                                                >  SRR4302540.3771709/1‑94 (MQ=60)
                                               CTTAACCGGCACACGAGAGCGCCAACAGCCCCAGAAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAA                                                <  SRR4302540.2337755/94‑1 (MQ=60)
                                                TTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAAC                                               <  SRR4302540.4928715/94‑1 (MQ=60)
                                                   ACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCG                                            <  SRR4302540.9638843/94‑1 (MQ=60)
                                                     CGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCC                                          >  SRR4302540.8498906/1‑94 (MQ=60)
                                                     CGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCC                                          <  SRR4302540.10092759/94‑1 (MQ=60)
                                                        GACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATC                                       <  SRR4302540.3046722/94‑1 (MQ=60)
                                                         ACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCT                                      >  SRR4302540.800807/1‑94 (MQ=60)
                                                         ACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCT                                      >  SRR4302540.3954795/1‑94 (MQ=60)
                                                         ACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCT                                      <  SRR4302540.8507224/94‑1 (MQ=60)
                                                          CACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTG                                     >  SRR4302540.7602073/1‑94 (MQ=60)
                                                          CACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTG                                     >  SRR4302540.3816724/1‑94 (MQ=60)
                                                          CACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTG                                     <  SRR4302540.3651822/94‑1 (MQ=60)
                                                           ACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGC                                    >  SRR4302540.8319771/1‑94 (MQ=60)
                                                           ACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGC                                    >  SRR4302540.1326924/1‑94 (MQ=60)
                                                            CGAGAGCGCCACCAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCA                                   <  SRR4302540.5443547/94‑1 (MQ=60)
                                                            CGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCA                                   >  SRR4302540.1103396/1‑94 (MQ=60)
                                                             GAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAA                                  >  SRR4302540.2352784/1‑94 (MQ=60)
                                                              AGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAA                                 <  SRR4302540.3539969/94‑1 (MQ=60)
                                                              AGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAA                                 >  SRR4302540.2005566/1‑94 (MQ=60)
                                                                AGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACC                               >  SRR4302540.3303752/1‑94 (MQ=60)
                                                                AGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACC                               <  SRR4302540.1143658/94‑1 (MQ=60)
                                                                   GCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAAT                            <  SRR4302540.7806540/94‑1 (MQ=60)
                                                                   GCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAAT                            >  SRR4302540.7217915/1‑94 (MQ=60)
                                                                   GCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAAT                            <  SRR4302540.7806538/94‑1 (MQ=60)
                                                                    CCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATG                           >  SRR4302540.2990989/1‑94 (MQ=60)
                                                                    CCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATG                           <  SRR4302540.7928397/94‑1 (MQ=60)
                                                                    CCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATG                           <  SRR4302540.8789545/94‑1 (MQ=60)
                                                                     CAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGT                          >  SRR4302540.4544290/1‑94 (MQ=60)
                                                                      AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCACACCAATGTT                         >  SRR4302540.520369/1‑94 (MQ=60)
                                                                      AACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTT                         <  SRR4302540.498450/94‑1 (MQ=60)
                                                                       ACAGCCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTT                        <  SRR4302540.221651/94‑1 (MQ=60)
                                                                       ACAGCCCCAGCAAGGAGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTCT                        >  SRR4302540.3772087/1‑94 (MQ=60)
                                                                       ACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTT                        >  SRR4302540.3772088/1‑94 (MQ=60)
                                                                        CAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTG                       <  SRR4302540.1204800/94‑1 (MQ=60)
                                                                        CAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTG                       <  SRR4302540.1733791/94‑1 (MQ=60)
                                                                        CAGACCCAGCAAGGCGCTCACGACATCACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTG                       <  SRR4302540.9503826/94‑1 (MQ=60)
                                                                         AGACCCAGCAAGTCCCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGA                      <  SRR4302540.2868297/94‑1 (MQ=60)
                                                                         AGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGA                      <  SRR4302540.8359780/94‑1 (MQ=60)
                                                                         AGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGA                      >  SRR4302540.7060941/1‑94 (MQ=60)
                                                                         AGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGA                      <  SRR4302540.5795246/94‑1 (MQ=60)
                                                                         AGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGA                      >  SRR4302540.2578653/1‑94 (MQ=60)
                                                                          agcccgGCAAGGCCCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGAT                     <  SRR4302540.2178612/88‑1 (MQ=60)
                                                                          accccacnAAGGCGCTCACGNNANTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGAT                     <  SRR4302540.2207332/86‑1 (MQ=60)
                                                                          GACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGAT                     <  SRR4302540.329218/94‑1 (MQ=60)
                                                                          GACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGAT                     >  SRR4302540.1784986/1‑94 (MQ=60)
                                                                            CCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGT                   >  SRR4302540.4389627/1‑94 (MQ=60)
                                                                             CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTT                  >  SRR4302540.2762787/1‑94 (MQ=60)
                                                                             CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTT                  >  SRR4302540.3561825/1‑94 (MQ=60)
                                                                             CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTT                  >  SRR4302540.610541/1‑94 (MQ=60)
                                                                              CAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTA                 >  SRR4302540.9745972/1‑94 (MQ=60)
                                                                              CAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTA                 >  SRR4302540.9745973/1‑94 (MQ=60)
                                                                               AGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAA                >  SRR4302540.5280370/1‑94 (MQ=60)
                                                                               AGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAA                <  SRR4302540.3667865/94‑1 (MQ=60)
                                                                               AGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAA                >  SRR4302540.8772060/1‑94 (MQ=60)
                                                                               AGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAA                >  SRR4302540.1049636/1‑94 (MQ=60)
                                                                                GCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAA               >  SRR4302540.9637362/1‑94 (MQ=60)
                                                                                GCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAA               >  SRR4302540.2995710/1‑94 (MQ=60)
                                                                                GCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAA               >  SRR4302540.5365396/1‑94 (MQ=60)
                                                                                GCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTAAAA               <  SRR4302540.8224639/94‑1 (MQ=60)
                                                                                GCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCAGCTGCAAACCAATGTTTGATGTTAAA               <  SRR4302540.4884372/94‑1 (MQ=60)
                                                                                 CAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAA              <  SRR4302540.4974218/94‑1 (MQ=60)
                                                                                 CAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAA              >  SRR4302540.3545761/1‑94 (MQ=60)
                                                                                 CAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAA              >  SRR4302540.4637926/1‑94 (MQ=60)
                                                                                 AAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAA              <  SRR4302540.4136561/94‑1 (MQ=60)
                                                                                  AAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAAC             >  SRR4302540.843586/1‑94 (MQ=60)
                                                                                  AAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAAC             <  SRR4302540.7364014/94‑1 (MQ=60)
                                                                                  AAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAAC             <  SRR4302540.8895152/94‑1 (MQ=60)
                                                                                  AAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAAC             <  SRR4302540.965166/94‑1 (MQ=60)
                                                                                   AGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACC            <  SRR4302540.9487295/94‑1 (MQ=60)
                                                                                    GGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCC           <  SRR4302540.2264225/94‑1 (MQ=60)
                                                                                    GGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCC           <  SRR4302540.9937941/94‑1 (MQ=60)
                                                                                    GGCGCCCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCC           <  SRR4302540.5842509/94‑1 (MQ=60)
                                                                                    GGCCCTCACGACATTACTGGAGCCTAAACAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCC           <  SRR4302540.2157719/94‑1 (MQ=60)
                                                                                      CGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGC         <  SRR4302540.5987661/94‑1 (MQ=60)
                                                                                       GCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAAACCGCC        >  SRR4302540.2978583/1‑94 (MQ=60)
                                                                                        CTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCA       <  SRR4302540.7875769/94‑1 (MQ=60)
                                                                                        CTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCA       >  SRR4302540.4771051/1‑94 (MQ=60)
                                                                                        CTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCA       >  SRR4302540.4697382/1‑94 (MQ=60)
                                                                                          CACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAAT     >  SRR4302540.5141169/1‑94 (MQ=60)
                                                                                          CACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAAT     <  SRR4302540.7331726/94‑1 (MQ=60)
                                                                                          CACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAAT     <  SRR4302540.676343/94‑1 (MQ=60)
                                                                                           ACGACATTGCTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAATT    <  SRR4302540.8715301/94‑1 (MQ=60)
                                                                                           ACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAATT    >  SRR4302540.896890/1‑94 (MQ=60)
                                                                                            CGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACTAATGTTTGATGTTAAAATCCGCCAATTC   >  SRR4302540.8670028/1‑94 (MQ=60)
                                                                                            CGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCAATCTGCAACCCAATGTTTGATTTTAAACCCCGCCATTTC   >  SRR4302540.2238254/1‑94 (MQ=60)
                                                                                             GACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTACAACCCGCCAATTCA  >  SRR4302540.1273427/1‑94 (MQ=60)
                                                                                             GACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAATTCA  >  SRR4302540.1273428/1‑94 (MQ=60)
                                                                                             GACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAATTCA  <  SRR4302540.3259338/94‑1 (MQ=60)
                                                                                             |                                                                                             
GTACGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACAACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAATTCA  >  NC_000913/3130241‑3130427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑