Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,340,190 A→G T93A (ACT→GCT)  yrbI → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,340,1900AG99.8% 1480.8 / ‑5.4 457T93A (ACT→GCT) yrbIhypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (207/248);  total (208/249)
Fisher's exact test for biased strand distribution p-value = 4.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.70e-01

Only 100 of 489 total aligned reads displayed.

CGTTGTGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCACTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTAT  >  NC_000913/3340100‑3340279
                                                                                          |                                                                                         
cGTTGTGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGAtcgct                                                                                         >  2:4523394/1‑93 (MQ=255)
 gTTGTGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGAtcgctc                                                                                        >  2:8114877/1‑93 (MQ=255)
 gTTGTGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGAtcgctc                                                                                        >  1:2848104/1‑93 (MQ=255)
  ttGTGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGAtcgctca                                                                                       <  2:9348871/93‑1 (MQ=255)
  ttGTGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGAtcgctca                                                                                       <  2:8284225/93‑1 (MQ=255)
   tgtgCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGAtcgctcac                                                                                      <  1:4429128/93‑1 (MQ=255)
    gtgCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATcgctcact                                                                                     <  1:3965227/93‑1 (MQ=255)
     tgCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACtt                                                                                    <  1:5976366/93‑1 (MQ=255)
     tgCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACtt                                                                                    >  2:726810/1‑93 (MQ=255)
      gcgcTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTg                                                                                   <  1:5310645/93‑1 (MQ=255)
      gcgcTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTg                                                                                   >  2:5634267/1‑93 (MQ=255)
        gcTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTa                                                                                 <  1:1912791/93‑1 (MQ=255)
        gcTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTa                                                                                 >  1:8873459/1‑93 (MQ=255)
          tCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATc                                                                               >  2:779143/1‑93 (MQ=255)
           cACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCa                                                                              <  1:7554709/93‑1 (MQ=255)
            cccTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTTTATCAg                                                                             <  2:4914747/92‑1 (MQ=255)
            aCCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAg                                                                             <  2:1815717/93‑1 (MQ=255)
            aCCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAg                                                                             >  1:2316532/1‑93 (MQ=255)
             ccTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAgg                                                                            >  2:2986391/1‑93 (MQ=255)
              ctctGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAggg                                                                           >  2:4302479/1‑93 (MQ=255)
              ctctGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAggg                                                                           >  1:577787/1‑93 (MQ=255)
              ctctGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAggg                                                                           >  1:1282414/1‑93 (MQ=255)
               tctGATATTGAAGTCTTGCTCATTTCCTGGCGAATGGCTAAGCTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAgggg                                                                          <  1:8692305/93‑1 (MQ=255)
               tctGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAgggg                                                                          <  1:144612/93‑1 (MQ=255)
                ctGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGc                                                                         <  1:2174593/93‑1 (MQ=255)
                 tGATATTGAAGTCGCTATCATTACTGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTTGGGATCGCTCACTTGTATCAGGGGcg                                                                        >  2:9205345/1‑92 (MQ=255)
                   atatTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGt                                                                      <  2:1067874/93‑1 (MQ=255)
                   atatTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGt                                                                      <  2:2891929/93‑1 (MQ=255)
                    tatTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGta                                                                     >  1:8162209/1‑92 (MQ=255)
                    tatTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTc                                                                     >  2:9714444/1‑93 (MQ=255)
                      ttGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTcaa                                                                   >  1:10035708/1‑93 (MQ=255)
                        gAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTcaaac                                                                 >  1:9647560/1‑93 (MQ=255)
                         aaGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTcaaaca                                                                >  1:673438/1‑93 (MQ=255)
                         aaGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTcaaaca                                                                <  2:6128141/93‑1 (MQ=255)
                         aaGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTcaaaca                                                                >  1:9429411/1‑93 (MQ=255)
                            tCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCaaacaaac                                                             >  1:2502527/1‑93 (MQ=255)
                            tCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCaaacaaac                                                             >  1:7290339/1‑93 (MQ=255)
                            tCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCaaacaaac                                                             <  2:6459191/93‑1 (MQ=255)
                            tCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCaaacaaac                                                             <  2:8705843/93‑1 (MQ=255)
                             cGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACt                                                            <  2:8515227/93‑1 (MQ=255)
                                tATCATTGCCGGGCGAAAGGCTAATCTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGAt                                                         <  2:3984635/93‑1 (MQ=255)
                                tATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGAt                                                         <  1:921767/93‑1 (MQ=255)
                                tATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGAt                                                         >  2:3739113/1‑93 (MQ=255)
                                tATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGAt                                                         <  1:5059967/93‑1 (MQ=255)
                                 aTCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATc                                                        >  1:5529328/1‑93 (MQ=255)
                                 aTCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATc                                                        <  2:5661318/93‑1 (MQ=255)
                                 aTCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATc                                                        >  2:258810/1‑93 (MQ=255)
                                  tCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCg                                                       >  1:7786293/1‑93 (MQ=255)
                                  tCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCg                                                       >  2:2215242/1‑93 (MQ=255)
                                    aTTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGcc                                                     <  2:4425539/93‑1 (MQ=255)
                                    aTTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGcc                                                     >  1:7111488/1‑93 (MQ=255)
                                     ttACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCt                                                    >  2:3518997/1‑93 (MQ=255)
                                     ttACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCt                                                    >  2:3306618/1‑93 (MQ=255)
                                       aCCGGGGGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCttt                                                  <  1:7442539/93‑1 (MQ=255)
                                       aCCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCttt                                                  <  1:3023083/93‑1 (MQ=255)
                                        ccGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTa                                                 <  2:9840401/93‑1 (MQ=255)
                                            gCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGa                                             <  2:6557419/93‑1 (MQ=255)
                                            gCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGa                                             <  2:7589744/93‑1 (MQ=255)
                                              gAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATc                                           >  2:893852/1‑93 (MQ=255)
                                               aaaGGCTAAACTGGTAGAAGGTCGTTGTTCCACATTGGGGATCGCTCACTTGTAGCAGGGGCAGTAAAACAAACTGATCGCCTTTAGCGATct                                          <  2:6226859/93‑1 (MQ=255)
                                               aaaGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATct                                          >  1:8998058/1‑93 (MQ=255)
                                                aaGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATctg                                         >  1:4279338/1‑93 (MQ=255)
                                                 aGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATctgc                                        <  2:9489786/93‑1 (MQ=255)
                                                  gtcTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATctgct                                       <  2:7111268/91‑1 (MQ=255)
                                                  ggCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATctgct                                       <  2:2381322/93‑1 (MQ=255)
                                                  ggCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATctgct                                       <  2:747048/93‑1 (MQ=255)
                                                   gCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATctgctg                                      <  2:3354229/93‑1 (MQ=255)
                                                     tAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGa                                    >  2:1219080/1‑93 (MQ=255)
                                                      aaaCTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAgggg                                                                          >  2:6909424/1‑54 (MQ=255)
                                                      aaaCTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGaa                                   >  1:1573922/1‑93 (MQ=255)
                                                      aaaCTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGaa                                   <  2:1961062/93‑1 (MQ=255)
                                                       aaCTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGaaa                                  >  1:4995971/1‑93 (MQ=255)
                                                       aaCTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGaaa                                  <  1:9080090/93‑1 (MQ=255)
                                                          tGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAAc                               >  2:3094717/1‑93 (MQ=255)
                                                          tGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAAc                               <  1:8484043/93‑1 (MQ=255)
                                                          tGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAAc                               >  2:505226/1‑93 (MQ=255)
                                                          tGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAAc                               <  1:3172683/93‑1 (MQ=255)
                                                           ggTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACt                              <  2:413886/93‑1 (MQ=255)
                                                           ggTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACt                              >  1:8031913/1‑93 (MQ=255)
                                                           ggTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACt                              >  1:3809402/1‑93 (MQ=255)
                                                           ggTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACt                              >  1:6044771/1‑93 (MQ=255)
                                                            gTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTg                             <  2:6029970/93‑1 (MQ=255)
                                                            gTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTg                             >  1:7657545/1‑93 (MQ=255)
                                                            gTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTg                             <  2:1658338/93‑1 (MQ=255)
                                                             tAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTgg                            <  1:3427605/93‑1 (MQ=255)
                                                              agaagaTCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGc                           <  2:135828/93‑1 (MQ=255)
                                                                aagaTCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGa                         <  2:8330395/93‑1 (MQ=255)
                                                                 agaTCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGAt                        <  1:6471021/93‑1 (MQ=255)
                                                                  gaTCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGAtt                       <  1:354972/93‑1 (MQ=255)
                                                                  gaTCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGAtt                       <  1:4810515/93‑1 (MQ=255)
                                                                   aTCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTg                      >  2:5876555/1‑93 (MQ=255)
                                                                   aTCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTg                      >  1:6837393/1‑93 (MQ=255)
                                                                          tgCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGa               <  1:2587997/93‑1 (MQ=255)
                                                                             cacaTTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGaaaa            <  2:2078978/93‑1 (MQ=255)
                                                                              acaTTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAAt           >  2:4793599/1‑93 (MQ=255)
                                                                               caTTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAAtg          <  1:2756764/93‑1 (MQ=255)
                                                                                 ttGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAAtgtg        >  1:63376/1‑93 (MQ=255)
                                                                                   ggggATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGc      >  1:6181277/1‑93 (MQ=255)
                                                                                      gATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTa   <  1:325216/93‑1 (MQ=255)
                                                                                      gATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTa   <  1:4525929/93‑1 (MQ=255)
                                                                                          |                                                                                         
CGTTGTGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCACTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTAT  >  NC_000913/3340100‑3340279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 260 total aligned reads displayed.
ATTCGTTGTGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCACTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATGTCG  >  NC_000913/3340097‑3340283
                                                                                             |                                                                                             
ATTCGTTGTGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCG                                                                                               >  SRR4302540.3731931/1‑94 (MQ=60)
ATTCGTTGTGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCG                                                                                               <  SRR4302540.4682569/94‑1 (MQ=60)
ATTCGTTGTGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCG                                                                                               >  SRR4302540.698113/1‑94 (MQ=60)
ATTCGTTGTGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCG                                                                                               >  SRR4302540.3222965/1‑94 (MQ=60)
ATTCGTTGTGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCG                                                                                               >  SRR4302540.8096422/1‑94 (MQ=60)
ATTCGTTGTGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCG                                                                                               >  SRR4302540.6456526/1‑94 (MQ=60)
 TTCGTTGTGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGC                                                                                              >  SRR4302540.1082354/1‑94 (MQ=60)
  TCGTTGTGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCT                                                                                             <  SRR4302540.7697139/94‑1 (MQ=60)
  TCGTTGTGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCT                                                                                             >  SRR4302540.6488501/1‑94 (MQ=60)
   CGTTGTGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTC                                                                                            >  SRR4302540.6393703/1‑94 (MQ=60)
   CGTTGTGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTC                                                                                            >  SRR4302540.2878943/1‑94 (MQ=60)
     TTGTGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCAC                                                                                          <  SRR4302540.5394845/94‑1 (MQ=60)
      TGTGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACT                                                                                         <  SRR4302540.4477117/94‑1 (MQ=60)
       GTGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTT                                                                                        <  SRR4302540.4007979/94‑1 (MQ=60)
        TGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTG                                                                                       <  SRR4302540.7412230/94‑1 (MQ=60)
        TGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTG                                                                                       >  SRR4302540.1980144/1‑94 (MQ=60)
        TGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTG                                                                                       <  SRR4302540.6042524/94‑1 (MQ=60)
        TGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTG                                                                                       <  SRR4302540.6042523/94‑1 (MQ=60)
        TGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCCCACTTG                                                                                       >  SRR4302540.3675785/1‑94 (MQ=60)
         GCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGT                                                                                      <  SRR4302540.5368774/94‑1 (MQ=60)
          CGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTA                                                                                     >  SRR4302540.8976153/1‑94 (MQ=60)
          CGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTA                                                                                     >  SRR4302540.3203884/1‑94 (MQ=60)
           GCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCGCATTGGGGATCGCTCACTTGTAT                                                                                    <  SRR4302540.3081178/94‑1 (MQ=60)
           GCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTAT                                                                                    <  SRR4302540.1933682/94‑1 (MQ=60)
           GCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTAT                                                                                    >  SRR4302540.1489998/1‑94 (MQ=60)
           GCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTAT                                                                                    >  SRR4302540.7036525/1‑94 (MQ=60)
           GCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTAT                                                                                    >  SRR4302540.7264777/1‑94 (MQ=60)
            CTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATC                                                                                   >  SRR4302540.6840894/1‑94 (MQ=60)
            CTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATC                                                                                   <  SRR4302540.6817449/94‑1 (MQ=60)
            CTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATC                                                                                   <  SRR4302540.5706985/94‑1 (MQ=60)
            CTCACCTCTGATATTGAAGGCGCTTTCATTTCCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATC                                                                                   <  SRR4302540.1454324/94‑1 (MQ=60)
            CTC‑CCTCTGAGATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCA                                                                                  <  SRR4302540.6201827/94‑1 (MQ=60)
             TCTCCTTTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACTCTGGGGATCGCTCACTTGTATCA                                                                                  >  SRR4302540.9093232/1‑94 (MQ=60)
             TCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCA                                                                                  <  SRR4302540.1422745/94‑1 (MQ=60)
             TCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCA                                                                                  <  SRR4302540.5707915/94‑1 (MQ=60)
             TCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCA                                                                                  >  SRR4302540.607812/1‑94 (MQ=60)
              cggCTCTGATGTTGAAGTGGCTATCATTACCGGGAGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAG                                                                                 <  SRR4302540.655327/91‑1 (MQ=60)
              CACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAG                                                                                 <  SRR4302540.655328/94‑1 (MQ=60)
              CACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAG                                                                                 <  SRR4302540.4558804/94‑1 (MQ=60)
              CACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAG                                                                                 <  SRR4302540.3261017/94‑1 (MQ=60)
               ACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGG                                                                                >  SRR4302540.9120120/1‑94 (MQ=60)
               ACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGG                                                                                >  SRR4302540.2927939/1‑94 (MQ=60)
               ACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGG                                                                                >  SRR4302540.5706321/1‑94 (MQ=60)
               ACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGCTGTGCCACATTGGGGATCGCTCACTTGTATCAGG                                                                                >  SRR4302540.9120118/1‑94 (MQ=60)
                CCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGG                                                                               <  SRR4302540.822355/94‑1 (MQ=60)
                CCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGG                                                                               >  SRR4302540.1295641/1‑94 (MQ=60)
                CCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGG                                                                               >  SRR4302540.1295640/1‑94 (MQ=60)
                 CTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGG                                                                              <  SRR4302540.7832482/94‑1 (MQ=60)
                 CTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGG                                                                              >  SRR4302540.5138631/1‑94 (MQ=60)
                  TCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGC                                                                             >  SRR4302540.7575758/1‑94 (MQ=60)
                  TCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGC                                                                             >  SRR4302540.6253155/1‑94 (MQ=60)
                   CTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCA                                                                            >  SRR4302540.2694132/1‑94 (MQ=60)
                   CTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCA                                                                            >  SRR4302540.2370360/1‑94 (MQ=60)
                   CTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCA                                                                            >  SRR4302540.8654248/1‑94 (MQ=60)
                   CTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCA                                                                            <  SRR4302540.2198046/94‑1 (MQ=60)
                    TGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAG                                                                           >  SRR4302540.9714560/1‑94 (MQ=60)
                     GATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGT                                                                          >  SRR4302540.5283042/1‑94 (MQ=60)
                     GATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGT                                                                          <  SRR4302540.7552070/94‑1 (MQ=60)
                     GATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGT                                                                          >  SRR4302540.9520511/1‑94 (MQ=60)
                      ATTTTGAAGTCTCTATCATTACCGGGCGAATGGCTAAACTGGTAGAAGATCGCTGTGCCATATTGTGGATCGCTTACCTGTATCAGGGGCAGTC                                                                         >  SRR4302540.9090285/1‑94 (MQ=57)
                      ATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTC                                                                         >  SRR4302540.7325363/1‑94 (MQ=60)
                      ATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTA                                                                         >  SRR4302540.8256988/1‑94 (MQ=60)
                       TATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCA                                                                        >  SRR4302540.4920768/1‑94 (MQ=60)
                        ATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAA                                                                       >  SRR4302540.10152425/1‑94 (MQ=60)
                        ATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAA                                                                       <  SRR4302540.9221833/94‑1 (MQ=60)
                          TGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAAC                                                                     >  SRR4302540.9761172/1‑94 (MQ=60)
                           GAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACA                                                                    >  SRR4302540.680647/1‑94 (MQ=60)
                           GAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACA                                                                    >  SRR4302540.9541434/1‑94 (MQ=60)
                           GAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGCGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACA                                                                    <  SRR4302540.9376967/94‑1 (MQ=60)
                              GTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAAC                                                                 >  SRR4302540.2529531/1‑94 (MQ=60)
                              GTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAAC                                                                 <  SRR4302540.7250677/94‑1 (MQ=60)
                              GTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAAC                                                                 >  SRR4302540.7371695/1‑94 (MQ=60)
                               TCGCTATCATTACCGGGCGAAAGGCTAAGCTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACT                                                                <  SRR4302540.6420200/94‑1 (MQ=60)
                               TCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACT                                                                <  SRR4302540.8762883/94‑1 (MQ=60)
                                CGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTG                                                               <  SRR4302540.7736818/94‑1 (MQ=60)
                                  CTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGAT                                                             >  SRR4302540.6146665/1‑94 (MQ=60)
                                  CTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGAT                                                             >  SRR4302540.4520160/1‑94 (MQ=60)
                                   TATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATC                                                            <  SRR4302540.5303217/94‑1 (MQ=60)
                                   TATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATC                                                            >  SRR4302540.2625132/1‑94 (MQ=60)
                                   TATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATC                                                            <  SRR4302540.9691461/94‑1 (MQ=60)
                                   TATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATC                                                            >  SRR4302540.5590228/1‑94 (MQ=60)
                                   TATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATC                                                            <  SRR4302540.5114674/94‑1 (MQ=60)
                                   TATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATC                                                            <  SRR4302540.87937/94‑1 (MQ=60)
                                   TATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATC                                                            <  SRR4302540.931491/94‑1 (MQ=60)
                                   TATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATC                                                            <  SRR4302540.3478137/94‑1 (MQ=60)
                                   TATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATC                                                            <  SRR4302540.1120559/94‑1 (MQ=60)
                                   TATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATC                                                            <  SRR4302540.3478136/94‑1 (MQ=60)
                                   TATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATC                                                            <  SRR4302540.931488/94‑1 (MQ=60)
                                    ATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCG                                                           >  SRR4302540.1479429/1‑94 (MQ=60)
                                    ATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCG                                                           >  SRR4302540.9600679/1‑94 (MQ=60)
                                    ATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCG                                                           <  SRR4302540.3321317/94‑1 (MQ=60)
                                    ATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCG                                                           >  SRR4302540.5097665/1‑94 (MQ=60)
                                    ATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCG                                                           >  SRR4302540.7877312/1‑94 (MQ=60)
                                    ATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCG                                                           >  SRR4302540.4768066/1‑94 (MQ=60)
                                      CATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCC                                                         >  SRR4302540.7190641/1‑94 (MQ=60)
                                       ATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCT                                                        >  SRR4302540.6967334/1‑94 (MQ=60)
                                       ATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCT                                                        <  SRR4302540.2042841/94‑1 (MQ=60)
                                       ATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCT                                                        >  SRR4302540.8265954/1‑94 (MQ=60)
                                        TTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTT                                                       >  SRR4302540.2218126/1‑94 (MQ=60)
                                          ACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTA                                                     <  SRR4302540.8036194/94‑1 (MQ=60)
                                          ACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTA                                                     <  SRR4302540.7525458/94‑1 (MQ=60)
                                          ACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTA                                                     <  SRR4302540.3056300/94‑1 (MQ=60)
                                           CCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAG                                                    <  SRR4302540.74914/94‑1 (MQ=60)
                                            CGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGC                                                   >  SRR4302540.5548570/1‑94 (MQ=60)
                                            CGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGC                                                   <  SRR4302540.4453920/94‑1 (MQ=60)
                                             GGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCG                                                  <  SRR4302540.4674374/94‑1 (MQ=60)
                                               GCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGAT                                                >  SRR4302540.3996668/1‑94 (MQ=60)
                                                CGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATC                                               >  SRR4302540.328002/1‑94 (MQ=60)
                                                 GAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCT                                              >  SRR4302540.9106004/1‑94 (MQ=60)
                                                 GAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCT                                              <  SRR4302540.248802/94‑1 (MQ=60)
                                                 GAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCT                                              <  SRR4302540.3415622/94‑1 (MQ=60)
                                                  AAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTG                                             >  SRR4302540.4325882/1‑94 (MQ=60)
                                                   AAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGC                                            >  SRR4302540.2828239/1‑94 (MQ=60)
                                                   AAGGCTAAACTGGTAGAAGAGCGTTGTGCCACATTGGGGATCGCTCACTTGGATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGC                                            <  SRR4302540.8631787/94‑1 (MQ=60)
                                                    aggacAAACTGGTAGAAGATCGTTGTCCCACAGTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCT                                           <  SRR4302540.3437415/89‑1 (MQ=60)
                                                    AGGCTAAACTTGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCT                                           >  SRR4302540.3037636/1‑94 (MQ=60)
                                                    AGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCT                                           <  SRR4302540.3328330/94‑1 (MQ=60)
                                                     GGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTG                                          >  SRR4302540.5483562/1‑94 (MQ=60)
                                                     GGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTG                                          >  SRR4302540.7039199/1‑94 (MQ=60)
                                                      GCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGG                                         <  SRR4302540.3287137/94‑1 (MQ=60)
                                                      GCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGG                                         <  SRR4302540.7842356/94‑1 (MQ=60)
                                                       CTAAACTGGTAGAAGATCGTTATGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGANCGCCTTTAGCGATCTGCTGGA                                        >  SRR4302540.2847719/1‑94 (MQ=60)
                                                        TAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAA                                       >  SRR4302540.8835855/1‑94 (MQ=60)
                                                        TAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAA                                       >  SRR4302540.1591156/1‑94 (MQ=60)
                                                         AAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAA                                      >  SRR4302540.3997564/1‑94 (MQ=60)
                                                         AAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAA                                      >  SRR4302540.5050056/1‑94 (MQ=60)
                                                          AACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAA                                     <  SRR4302540.9189167/94‑1 (MQ=60)
                                                           ACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAA                                    >  SRR4302540.3160551/1‑94 (MQ=60)
                                                            CTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAAC                                   >  SRR4302540.8129158/1‑94 (MQ=60)
                                                            CTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAAC                                   <  SRR4302540.6056195/94‑1 (MQ=60)
                                                             TGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACT                                  >  SRR4302540.6111917/1‑94 (MQ=60)
                                                             TGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACT                                  <  SRR4302540.185751/94‑1 (MQ=60)
                                                             TGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACT                                  >  SRR4302540.8527611/1‑94 (MQ=60)
                                                             TGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACT                                  <  SRR4302540.8582599/94‑1 (MQ=60)
                                                             TGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACT                                  >  SRR4302540.3850890/1‑94 (MQ=60)
                                                             TGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACT                                  <  SRR4302540.185748/94‑1 (MQ=60)
                                                              GGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTG                                 >  SRR4302540.7743764/1‑94 (MQ=60)
                                                                TAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGC                               <  SRR4302540.6834363/94‑1 (MQ=60)
                                                                TAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGC                               <  SRR4302540.6441183/94‑1 (MQ=60)
                                                                TAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGC                               <  SRR4302540.3464856/94‑1 (MQ=60)
                                                                 AGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCG                              <  SRR4302540.8018072/94‑1 (MQ=60)
                                                                 AGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCG                              <  SRR4302540.7993863/94‑1 (MQ=60)
                                                                 AGAAGATCGGTGTGCCACATCGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCG                              <  SRR4302540.1904480/94‑1 (MQ=60)
                                                                  GAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGA                             <  SRR4302540.2474125/94‑1 (MQ=60)
                                                                  GAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGA                             >  SRR4302540.3471521/1‑94 (MQ=60)
                                                                   AAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGAT                            <  SRR4302540.5103230/94‑1 (MQ=60)
                                                                   AAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGAT                            <  SRR4302540.9412547/94‑1 (MQ=60)
                                                                     GATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTG                          >  SRR4302540.6913124/1‑94 (MQ=60)
                                                                     GATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTG                          >  SRR4302540.4309765/1‑94 (MQ=60)
                                                                     GATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTG                          >  SRR4302540.4930935/1‑94 (MQ=60)
                                                                     GATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTG                          <  SRR4302540.4862003/94‑1 (MQ=60)
                                                                     GATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTG                          >  SRR4302540.2255140/1‑94 (MQ=60)
                                                                     GATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTG                          <  SRR4302540.358616/94‑1 (MQ=60)
                                                                      ATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGC                         >  SRR4302540.5015420/1‑94 (MQ=60)
                                                                      ATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGC                         >  SRR4302540.1284005/1‑94 (MQ=60)
                                                                       TCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCC                        <  SRR4302540.4073787/94‑1 (MQ=60)
                                                                        CGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCC                       <  SRR4302540.3279256/94‑1 (MQ=60)
                                                                        CGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCC                       >  SRR4302540.3877165/1‑94 (MQ=60)
                                                                         GTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCC                      <  SRR4302540.4311474/94‑1 (MQ=60)
                                                                         GTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCC                      <  SRR4302540.1007523/94‑1 (MQ=60)
                                                                         GTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCC                      <  SRR4302540.3049007/94‑1 (MQ=60)
                                                                         GTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCC                      >  SRR4302540.4553876/1‑94 (MQ=60)
                                                                          TTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCCGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCG                     >  SRR4302540.2541662/1‑94 (MQ=60)
                                                                          TTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCG                     >  SRR4302540.2541661/1‑94 (MQ=60)
                                                                          TTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCG                     <  SRR4302540.7725735/94‑1 (MQ=60)
                                                                           TGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGG                    >  SRR4302540.879096/1‑94 (MQ=60)
                                                                           TGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGG                    <  SRR4302540.6624262/94‑1 (MQ=60)
                                                                           TGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGG                    <  SRR4302540.5939330/94‑1 (MQ=60)
                                                                            GTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGA                   >  SRR4302540.2218460/1‑94 (MQ=60)
                                                                             TGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAA                  <  SRR4302540.9247840/94‑1 (MQ=60)
                                                                             TGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAA                  <  SRR4302540.2615897/94‑1 (MQ=60)
                                                                             TGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAA                  <  SRR4302540.2624545/94‑1 (MQ=60)
                                                                              GCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAA                 >  SRR4302540.5057641/1‑94 (MQ=60)
                                                                               CCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCTGAAAC                >  SRR4302540.1519521/1‑94 (MQ=60)
                                                                               CCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAA                >  SRR4302540.5940120/1‑94 (MQ=60)
                                                                               CCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAA                <  SRR4302540.6376557/94‑1 (MQ=60)
                                                                               CCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGCAAAA                >  SRR4302540.1519523/1‑94 (MQ=60)
                                                                                CACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAAT               >  SRR4302540.2047707/1‑94 (MQ=60)
                                                                                CACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAAT               <  SRR4302540.7567316/94‑1 (MQ=60)
                                                                                 ACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATG              <  SRR4302540.7655911/94‑1 (MQ=60)
                                                                                  CATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGT             <  SRR4302540.2786553/94‑1 (MQ=60)
                                                                                  CATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGT             <  SRR4302540.7810214/94‑1 (MQ=60)
                                                                                   ATTGGGGATCTCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTG            <  SRR4302540.440511/94‑1 (MQ=60)
                                                                                   ATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATGTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTG            <  SRR4302540.6148506/94‑1 (MQ=60)
                                                                                   ATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTG            >  SRR4302540.8260011/1‑94 (MQ=60)
                                                                                   ATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTG            >  SRR4302540.64014/1‑94 (MQ=60)
                                                                                     TGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGC          >  SRR4302540.6250126/1‑94 (MQ=60)
                                                                                     TGGGGATCGCTCACTTGTATCAGGGGCAGGCAAACAAACTGATCGCCGTTAGCGATCTGCTGGAAAAACTGGCGATCGCCCCGGAAAACGTGGC          >  SRR4302540.9051429/1‑94 (MQ=60)
                                                                                      GGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCT         <  SRR4302540.2105737/94‑1 (MQ=60)
                                                                                      GGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCT         <  SRR4302540.2556863/94‑1 (MQ=60)
                                                                                        GGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTA       >  SRR4302540.3842775/1‑94 (MQ=60)
                                                                                         GATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTAT      <  SRR4302540.4574932/94‑1 (MQ=60)
                                                                                         GATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTAT      <  SRR4302540.8178191/94‑1 (MQ=60)
                                                                                           TCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATGT    <  SRR4302540.8937838/94‑1 (MQ=60)
                                                                                           TCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATGT    <  SRR4302540.3151226/94‑1 (MQ=60)
                                                                                            CGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATGTC   <  SRR4302540.5952332/94‑1 (MQ=60)
                                                                                            CGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATGTC   >  SRR4302540.3884494/1‑94 (MQ=60)
                                                                                            CGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATGTC   >  SRR4302540.7740472/1‑94 (MQ=60)
                                                                                             GCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATGTCG  <  SRR4302540.1436367/94‑1 (MQ=60)
                                                                                             GCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGATGGAAAAACTGACGATTGCCCCGGAAAATGTGGCTTATGTCG  >  SRR4302540.8666333/1‑94 (MQ=60)
                                                                                             |                                                                                             
ATTCGTTGTGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCACTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATGTCG  >  NC_000913/3340097‑3340283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑