Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,376,394 T→C intergenic (‑108/+111) rplM ← / ← yhcM 50S ribosomal subunit protein L13/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,376,3940TC99.8% 1470.2 / ‑5.6 449intergenic (‑108/+111)rplM/yhcM50S ribosomal subunit protein L13/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (197/251);  total (198/251)
Fisher's exact test for biased strand distribution p-value = 4.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 458 total aligned reads displayed.

AATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAGATCGACCCCT  >  NC_000913/3376304‑3376483
                                                                                          |                                                                                         
 ataGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGcc                                                                                        >  2:4218476/1‑93 (MQ=255)
  taGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCa                                                                                       >  1:7532546/1‑93 (MQ=255)
  taGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTGCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCa                                                                                       <  1:9802360/93‑1 (MQ=255)
    gTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGc                                                                                     <  1:6366246/93‑1 (MQ=255)
     ttACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGca                                                                                    <  2:4731662/93‑1 (MQ=255)
      tACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGcac                                                                                   >  2:124914/1‑93 (MQ=255)
      tACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGcaa                                                                                   >  2:9601191/1‑92 (MQ=255)
       acacGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGcaca                                                                                  <  1:7890655/93‑1 (MQ=255)
        cacGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGcacac                                                                                 <  1:1366545/93‑1 (MQ=255)
         acGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGcacaca                                                                                >  1:5920504/1‑93 (MQ=255)
         acGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGcacaca                                                                                >  2:5741310/1‑93 (MQ=255)
         acGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGcacaca                                                                                >  2:10032708/1‑93 (MQ=255)
           gTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACaaa                                                                              >  2:666655/1‑93 (MQ=255)
            ttGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACaaaa                                                                             >  2:6029856/1‑93 (MQ=255)
            ttGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACaaaa                                                                             <  1:8252291/93‑1 (MQ=255)
            gtGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACaaaa                                                                             <  1:2813993/92‑1 (MQ=255)
              ggTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAg                                                                           >  1:5576500/1‑93 (MQ=255)
              ggTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACCCCAGCACACAAAAAg                                                                           >  2:2205305/1‑93 (MQ=255)
               gTGATCACCCAAACGTCTTTAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGt                                                                          <  1:2124284/93‑1 (MQ=255)
               gTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGt                                                                          >  2:5441949/1‑93 (MQ=255)
               gTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGt                                                                          >  2:7203232/1‑93 (MQ=255)
                tGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGtt                                                                         <  2:7985706/93‑1 (MQ=255)
                tGAACACCCAAACGTCGTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGtt                                                                         <  1:2640442/93‑1 (MQ=255)
                 gAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGttt                                                                        >  2:8422936/1‑93 (MQ=255)
                    cacCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTgg                                                                     >  1:4857746/1‑93 (MQ=255)
                    cacCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTgg                                                                     >  2:406475/1‑93 (MQ=255)
                       ccAAACGTCTTCAATTGTTGAGGTTCACACGACCAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGaa                                                                  <  1:9705847/93‑1 (MQ=255)
                        cAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGaaa                                                                 <  1:4101366/93‑1 (MQ=255)
                         aaaCGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGaaaa                                                                <  2:153288/93‑1 (MQ=255)
                          aaCGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGaaaaa                                                               <  2:925767/93‑1 (MQ=255)
                           aCGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGaaaaaa                                                              <  1:39979/93‑1 (MQ=255)
                            cggcTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGaaaaaaa                                                             <  1:9054955/90‑1 (MQ=255)
                             gTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAAc                                                            <  2:7648371/93‑1 (MQ=255)
                             gTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAAc                                                            <  1:4493170/93‑1 (MQ=255)
                              tCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACt                                                           >  2:8121638/1‑93 (MQ=255)
                              tCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACt                                                           >  1:1118730/1‑93 (MQ=255)
                               cTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACtt                                                          <  1:8148212/93‑1 (MQ=255)
                               cTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACtt                                                          <  1:3336369/93‑1 (MQ=255)
                                ttCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACttt                                                         <  2:9723238/93‑1 (MQ=255)
                                ttCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACttt                                                         <  2:4750407/93‑1 (MQ=255)
                                 tCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTc                                                        <  2:2865384/93‑1 (MQ=255)
                                  cAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCt                                                       >  1:921901/1‑93 (MQ=255)
                                  cAATTGTTGAGGCTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCt                                                       <  2:5826027/93‑1 (MQ=255)
                                   aaTTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCtt                                                      <  2:7452330/93‑1 (MQ=255)
                                   aaTTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCtt                                                      >  2:3093024/1‑93 (MQ=255)
                                   aaTTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCtt                                                      >  2:6774483/1‑93 (MQ=255)
                                   aaTTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCtt                                                      <  1:6259418/93‑1 (MQ=255)
                                    aTTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTg                                                     <  1:6899156/93‑1 (MQ=255)
                                    aTTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTg                                                     >  2:8268120/1‑93 (MQ=255)
                                      tgttgAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTa                                                   <  1:5170522/93‑1 (MQ=255)
                                      tgttgAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTa                                                   <  1:8785934/93‑1 (MQ=255)
                                        ttgAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAAc                                                 <  1:5794411/93‑1 (MQ=255)
                                        ttgAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAAc                                                 <  2:5919849/93‑1 (MQ=255)
                                         tgAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACg                                                <  2:2489515/93‑1 (MQ=255)
                                          gAGGTGCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGt                                               <  1:8233818/93‑1 (MQ=255)
                                           aGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTg                                              <  1:2708959/93‑1 (MQ=255)
                                            ggTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTgg                                             <  1:9511699/93‑1 (MQ=255)
                                            ggTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTgg                                             >  1:9386070/1‑93 (MQ=255)
                                             gTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTggg                                            <  1:1570399/93‑1 (MQ=255)
                                             gTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTggg                                            <  2:3445644/93‑1 (MQ=255)
                                             gTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTggg                                            <  1:3143661/93‑1 (MQ=255)
                                              ttCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTgggg                                           >  1:8461715/1‑93 (MQ=255)
                                                cacaCGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTc                                         <  1:2762219/93‑1 (MQ=255)
                                                 acacGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCg                                        >  2:1347636/1‑93 (MQ=255)
                                                   acGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCa                                      >  2:9934142/1‑93 (MQ=255)
                                                    cGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAg                                     <  2:1826138/93‑1 (MQ=255)
                                                      aCAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGa                                   >  2:8346983/1‑93 (MQ=255)
                                                      aCAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGa                                   >  1:8878236/1‑93 (MQ=255)
                                                      aCAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGa                                   >  2:5603380/1‑93 (MQ=255)
                                                        aaaGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGAtt                                 <  1:3863377/93‑1 (MQ=255)
                                                         aaGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATta                                >  1:2342125/1‑93 (MQ=255)
                                                          aGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATtat                               <  1:10013401/93‑1 (MQ=255)
                                                          aGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATtat                               >  2:7729425/1‑93 (MQ=255)
                                                           gTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATtata                              <  1:7682285/93‑1 (MQ=255)
                                                            tCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATag                             <  1:3780651/93‑1 (MQ=255)
                                                              cGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATagag                           <  1:4684616/93‑1 (MQ=255)
                                                                gCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGaa                         >  2:5005188/1‑93 (MQ=255)
                                                                gCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGaa                         <  2:3400658/93‑1 (MQ=255)
                                                                gCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGaa                         <  1:1862206/93‑1 (MQ=255)
                                                                gCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGaa                         <  2:6919773/93‑1 (MQ=255)
                                                                gCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGaa                         <  2:2134817/93‑1 (MQ=255)
                                                                  aaaCCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGt                       >  2:2015828/1‑93 (MQ=255)
                                                                  aaaCCTACTCCTTCGAATAGCCTATGCCAGCACACAAAAATTTTTGTGAAAAAAACTGTCTTGTAACGTGGGGTCGCAGGATTAAAGAAAAGt                       <  2:9013820/93‑1 (MQ=255)
                                                                     ccTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCgg                    <  1:5405698/93‑1 (MQ=255)
                                                                     ccTACCCCTTCAAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCgg                    <  1:7205516/93‑1 (MQ=255)
                                                                      ctcccccTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCggg                   <  1:8617885/90‑1 (MQ=255)
                                                                       tcccccTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCgggg                  <  1:602319/91‑1 (MQ=255)
                                                                         ccccTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTc                >  1:6533862/1‑93 (MQ=255)
                                                                          cccTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCa               >  1:6789453/1‑93 (MQ=255)
                                                                          cccTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCa               >  1:8428716/1‑93 (MQ=255)
                                                                            cTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCaaa             >  2:4520249/1‑93 (MQ=255)
                                                                                gAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAGATc         >  2:3660459/1‑93 (MQ=255)
                                                                                gAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAGATc         <  2:3873735/93‑1 (MQ=255)
                                                                                 aaTAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAGATCg        >  1:7082087/1‑93 (MQ=255)
                                                                                  aTAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAGATCGa       <  2:5081685/93‑1 (MQ=255)
                                                                                   tAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAGATCGAc      <  2:2363388/93‑1 (MQ=255)
                                                                                   tAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAGATCGAc      >  1:3570155/1‑93 (MQ=255)
                                                                                    aGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAGATCGAcc     <  2:6387325/93‑1 (MQ=255)
                                                                                     gCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAGATCGAccc    <  2:2634189/93‑1 (MQ=255)
                                                                                       cTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAGATCGACCCCt  >  2:8633688/1‑93 (MQ=255)
                                                                                          |                                                                                         
AATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAGATCGACCCCT  >  NC_000913/3376304‑3376483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 244 total aligned reads displayed.
ATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAGATCGACCCCTTTTT  >  NC_000913/3376301‑3376487
                                                                                             |                                                                                             
ATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTAC                                                                                               <  SRR4302540.10115955/94‑1 (MQ=60)
 TAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACG                                                                                              <  SRR4302540.567352/94‑1 (MQ=60)
 TAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACG                                                                                              <  SRR4302540.8489150/94‑1 (MQ=60)
 TAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACG                                                                                              <  SRR4302540.8952074/94‑1 (MQ=60)
 AAAATAGTTTCACGTTGGTGAACACCCAAACGTCGTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACG                                                                                              <  SRR4302540.8489149/94‑1 (MQ=60)
  AAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGC                                                                                             >  SRR4302540.6916953/1‑94 (MQ=60)
  AAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGC                                                                                             <  SRR4302540.7001058/94‑1 (MQ=60)
  AAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGC                                                                                             >  SRR4302540.6066976/1‑94 (MQ=60)
   AATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCC                                                                                            <  SRR4302540.5243352/94‑1 (MQ=60)
   AATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCC                                                                                            <  SRR4302540.3975754/94‑1 (MQ=60)
   AATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCC                                                                                            >  SRR4302540.4922601/1‑94 (MQ=60)
    ATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCA                                                                                           >  SRR4302540.7616890/1‑94 (MQ=60)
     TAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTGCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAG                                                                                          <  SRR4302540.9917118/94‑1 (MQ=60)
     AAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAG                                                                                          <  SRR4302540.8857005/94‑1 (MQ=60)
      AGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTTCGCCAGC                                                                                         <  SRR4302540.1341814/94‑1 (MQ=60)
      AGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGC                                                                                         >  SRR4302540.3954435/1‑94 (MQ=60)
      AGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGC                                                                                         <  SRR4302540.9124561/94‑1 (MQ=60)
       GTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCA                                                                                        >  SRR4302540.934609/1‑94 (MQ=60)
       GTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCA                                                                                        >  SRR4302540.7946627/1‑94 (MQ=60)
       GTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCA                                                                                        <  SRR4302540.6437132/94‑1 (MQ=60)
        TTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCAC                                                                                       <  SRR4302540.7639599/94‑1 (MQ=60)
         TACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACA                                                                                      >  SRR4302540.7192536/1‑94 (MQ=60)
         TACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACA                                                                                      <  SRR4302540.7763587/94‑1 (MQ=60)
          ACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACAC                                                                                     >  SRR4302540.2816729/1‑94 (MQ=60)
          ACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACAC                                                                                     <  SRR4302540.7982749/94‑1 (MQ=60)
           CACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACA                                                                                    >  SRR4302540.5985912/1‑94 (MQ=60)
           CACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACA                                                                                    <  SRR4302540.1381009/94‑1 (MQ=60)
           CACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACA                                                                                    >  SRR4302540.4372218/1‑94 (MQ=60)
            ACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAA                                                                                   <  SRR4302540.833813/94‑1 (MQ=60)
            ACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCACCCTTCGAATAGCCTACGCCAGCACACAA                                                                                   <  SRR4302540.8906441/94‑1 (MQ=60)
             CGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAA                                                                                  <  SRR4302540.1216306/94‑1 (MQ=60)
              GTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAA                                                                                 >  SRR4302540.4775476/1‑94 (MQ=60)
              GTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAA                                                                                 >  SRR4302540.8466929/1‑94 (MQ=60)
                TGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAG                                                                               <  SRR4302540.410721/94‑1 (MQ=60)
                 GGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGT                                                                              <  SRR4302540.8965316/94‑1 (MQ=60)
                 GGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGT                                                                              <  SRR4302540.1974451/94‑1 (MQ=60)
                 GGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGT                                                                              <  SRR4302540.1726821/94‑1 (MQ=60)
                  GTGATCACCCAAACGTCTTTAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTT                                                                             <  SRR4302540.2147077/94‑1 (MQ=60)
                   TGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTT                                                                            <  SRR4302540.829721/94‑1 (MQ=60)
                   TGAACACCCAAACGTCGTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTT                                                                            <  SRR4302540.2669061/94‑1 (MQ=60)
                      ACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGG                                                                         >  SRR4302540.4909823/1‑94 (MQ=60)
                      ACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGG                                                                         >  SRR4302540.3170213/1‑94 (MQ=60)
                       CACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGG                                                                        <  SRR4302540.3388079/94‑1 (MQ=60)
                       CACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGG                                                                        <  SRR4302540.4692923/94‑1 (MQ=60)
                        ACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGA                                                                       >  SRR4302540.4726550/1‑94 (MQ=60)
                        ACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGA                                                                       >  SRR4302540.2420537/1‑94 (MQ=60)
                        ACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGA                                                                       >  SRR4302540.9582327/1‑94 (MQ=60)
                         CCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAA                                                                      <  SRR4302540.5132568/94‑1 (MQ=60)
                          CCAAACGTCTTCAATTGTTGAGGTTCACACGACCAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAA                                                                     <  SRR4302540.9819924/94‑1 (MQ=60)
                          CCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAA                                                                     <  SRR4302540.8084242/94‑1 (MQ=60)
                          CCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAA                                                                     <  SRR4302540.6528621/94‑1 (MQ=60)
                          CCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGCTTTGGGAAA                                                                     >  SRR4302540.530274/1‑94 (MQ=60)
                           CAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAA                                                                    <  SRR4302540.6703726/94‑1 (MQ=60)
                           CAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAA                                                                    <  SRR4302540.4145614/94‑1 (MQ=60)
                            AAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAA                                                                   >  SRR4302540.950432/1‑94 (MQ=60)
                             AACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAA                                                                  >  SRR4302540.7152977/1‑94 (MQ=60)
                               CGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAAT                                                                >  SRR4302540.8215469/1‑94 (MQ=60)
                               CGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAAC                                                                <  SRR4302540.9163658/94‑1 (MQ=60)
                               CGTCTTCAAGTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAAC                                                                <  SRR4302540.4454955/94‑1 (MQ=60)
                               CGGCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAAC                                                                <  SRR4302540.9163659/94‑1 (MQ=60)
                                GTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACT                                                               <  SRR4302540.4541888/94‑1 (MQ=60)
                                GTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACT                                                               >  SRR4302540.1130337/1‑94 (MQ=60)
                                 CCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTT                                                              <  SRR4302540.4775173/94‑1 (MQ=60)
                                  CTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGACTAGCCTACGCCCGCACACAACACGTTTTGGGACAAAAACTTT                                                             >  SRR4302540.8534556/1‑94 (MQ=60)
                                  CTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTT                                                             <  SRR4302540.3373007/94‑1 (MQ=60)
                                  CTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTT                                                             <  SRR4302540.8242802/94‑1 (MQ=60)
                                  CTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTT                                                             >  SRR4302540.2263939/1‑94 (MQ=60)
                                  CTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTT                                                             <  SRR4302540.8242800/94‑1 (MQ=60)
                                  CTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTT                                                             <  SRR4302540.2542986/94‑1 (MQ=60)
                                  CTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTT                                                             >  SRR4302540.325800/1‑94 (MQ=60)
                                  CTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTT                                                             >  SRR4302540.8534559/1‑94 (MQ=60)
                                  CTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTT                                                             >  SRR4302540.6124703/1‑94 (MQ=60)
                                   TTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTC                                                            <  SRR4302540.4084584/94‑1 (MQ=60)
                                    TCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCT                                                           >  SRR4302540.1367886/1‑94 (MQ=60)
                                    TCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCT                                                           >  SRR4302540.931625/1‑94 (MQ=60)
                                     CAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTT                                                          <  SRR4302540.5725507/94‑1 (MQ=60)
                                     CAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTT                                                          <  SRR4302540.3903629/94‑1 (MQ=60)
                                      AATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTG                                                         <  SRR4302540.2705820/94‑1 (MQ=60)
                                      AATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTG                                                         <  SRR4302540.7174556/94‑1 (MQ=60)
                                      AATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTG                                                         <  SRR4302540.6329061/94‑1 (MQ=60)
                                      AATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTG                                                         <  SRR4302540.3675096/94‑1 (MQ=60)
                                       ATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGT                                                        <  SRR4302540.6975782/94‑1 (MQ=60)
                                        TTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTA                                                       <  SRR4302540.3881109/94‑1 (MQ=60)
                                         TGTTGAGGTTCACACGACAAAGTCCGGCAAACCTTCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAA                                                      <  SRR4302540.10062344/94‑1 (MQ=60)
                                         TGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAA                                                      <  SRR4302540.6072729/94‑1 (MQ=60)
                                         TGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAA                                                      <  SRR4302540.8887615/94‑1 (MQ=60)
                                         TGTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAA                                                      <  SRR4302540.5226780/94‑1 (MQ=60)
                                          GTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAAC                                                     >  SRR4302540.8026639/1‑94 (MQ=60)
                                          GTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAAC                                                     <  SRR4302540.7386281/94‑1 (MQ=60)
                                          GTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAAC                                                     <  SRR4302540.3773881/94‑1 (MQ=60)
                                          GTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAAC                                                     <  SRR4302540.9916912/94‑1 (MQ=60)
                                          GTTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAAC                                                     >  SRR4302540.609374/1‑94 (MQ=60)
                                           TTGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACG                                                    <  SRR4302540.5858440/94‑1 (MQ=60)
                                            TGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGT                                                   >  SRR4302540.7979112/1‑94 (MQ=60)
                                            TGAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGT                                                   <  SRR4302540.3540756/94‑1 (MQ=60)
                                             GAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTG                                                  >  SRR4302540.5552151/1‑94 (MQ=60)
                                             GAGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTG                                                  >  SRR4302540.5552152/1‑94 (MQ=60)
                                             GAGGTGCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTG                                                  <  SRR4302540.8329525/94‑1 (MQ=60)
                                              AGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGG                                                 >  SRR4302540.6638660/1‑94 (MQ=60)
                                              AGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGG                                                 <  SRR4302540.2738172/94‑1 (MQ=60)
                                              AGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGG                                                 >  SRR4302540.9497788/1‑94 (MQ=60)
                                              AGGTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGG‑AAAAAACTTTCTTGTAACGTGGG                                                <  SRR4302540.10154296/94‑1 (MQ=60)
                                                GTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAACGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGG                                               >  SRR4302540.9782789/1‑94 (MQ=60)
                                                GTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAAGGTGGGG                                               >  SRR4302540.9217242/1‑94 (MQ=60)
                                                GTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGG                                               <  SRR4302540.2801715/94‑1 (MQ=60)
                                                GTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGG                                               >  SRR4302540.6338939/1‑94 (MQ=60)
                                                GTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGG                                               >  SRR4302540.6338940/1‑94 (MQ=60)
                                                GTTCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGG                                               <  SRR4302540.2010323/94‑1 (MQ=60)
                                                GTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGG                                               <  SRR4302540.3177924/94‑1 (MQ=60)
                                                  TCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAACCTTTCTTGTAACGTGGGGTC                                             <  SRR4302540.8339507/94‑1 (MQ=60)
                                                  TCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTC                                             <  SRR4302540.5561333/94‑1 (MQ=60)
                                                  TCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTC                                             >  SRR4302540.7703409/1‑94 (MQ=60)
                                                  TCACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTC                                             <  SRR4302540.8339506/94‑1 (MQ=60)
                                                   CACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCG                                            <  SRR4302540.4217202/94‑1 (MQ=60)
                                                   CACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCG                                            <  SRR4302540.2792064/94‑1 (MQ=60)
                                                   CACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCG                                            <  SRR4302540.3223020/94‑1 (MQ=60)
                                                   CACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCG                                            <  SRR4302540.4643651/94‑1 (MQ=60)
                                                    ACACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGC                                           >  SRR4302540.9753325/1‑94 (MQ=60)
                                                      ACGACAAAGTCCGGCACACCCCCCCCTTCGAATAGCCTACGCCAGCACACAAGAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAG                                         <  SRR4302540.8846096/94‑1 (MQ=60)
                                                      ACGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAG                                         <  SRR4302540.1681245/94‑1 (MQ=60)
                                                       CGACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGG                                        <  SRR4302540.721980/94‑1 (MQ=60)
                                                        GACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGA                                       >  SRR4302540.9913023/1‑94 (MQ=60)
                                                        GACAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGA                                       >  SRR4302540.8980978/1‑94 (MQ=60)
                                                          CAAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATT                                     <  SRR4302540.3927953/94‑1 (MQ=60)
                                                          CAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATT                                     >  SRR4302540.8270378/1‑94 (MQ=60)
                                                           AAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTA                                    >  SRR4302540.2810376/1‑94 (MQ=60)
                                                           AAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTA                                    >  SRR4302540.2367363/1‑94 (MQ=60)
                                                           AAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTA                                    <  SRR4302540.1070194/94‑1 (MQ=60)
                                                           AAAGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTA                                    <  SRR4302540.3663633/94‑1 (MQ=60)
                                                            AAGGCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTAT                                   <  SRR4302540.8677893/94‑1 (MQ=60)
                                                             AGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATA                                  >  SRR4302540.2252336/1‑94 (MQ=60)
                                                             AGTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATA                                  <  SRR4302540.10129981/94‑1 (MQ=60)
                                                             AGTCCCGCAAACCCCCCCCTTCGAATAGCCTACGCCAGCCCACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATA                                  <  SRR4302540.8573784/94‑1 (MQ=60)
                                                              GTCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAG                                 <  SRR4302540.7769299/94‑1 (MQ=60)
                                                               TCCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGA                                <  SRR4302540.3821890/94‑1 (MQ=60)
                                                                CCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAG                               <  SRR4302540.6555432/94‑1 (MQ=60)
                                                                CCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAG                               <  SRR4302540.6752421/94‑1 (MQ=60)
                                                                CCGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTCGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAG                               >  SRR4302540.5154343/1‑94 (MQ=60)
                                                                 CGGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGA                              <  SRR4302540.4734723/94‑1 (MQ=60)
                                                                  GGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAA                             >  SRR4302540.2764392/1‑94 (MQ=60)
                                                                  GGCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAA                             >  SRR4302540.4112241/1‑94 (MQ=60)
                                                                   GCAAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAG                            <  SRR4302540.264489/94‑1 (MQ=60)
                                                                   GCAAACCTACCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAG                            <  SRR4302540.1648504/94‑1 (MQ=60)
                                                                     aaaccgcccaCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTC                          <  SRR4302540.3985094/84‑1 (MQ=60)
                                                                     AAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTC                          <  SRR4302540.5347912/94‑1 (MQ=60)
                                                                     AAACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTC                          <  SRR4302540.2753923/94‑1 (MQ=60)
                                                                      AGCCTCCCCCTTCGAAGAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCG                         <  SRR4302540.3140896/94‑1 (MQ=60)
                                                                      AACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAATGTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCG                         <  SRR4302540.9461290/94‑1 (MQ=60)
                                                                      AACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCG                         <  SRR4302540.9461289/94‑1 (MQ=60)
                                                                      AACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCG                         <  SRR4302540.8677739/94‑1 (MQ=60)
                                                                       ACCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGG                        >  SRR4302540.8654416/1‑94 (MQ=60)
                                                                        CCTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGG                       <  SRR4302540.6919916/94‑1 (MQ=60)
                                                                        CCTACCCCTTCAAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGG                       <  SRR4302540.7285844/94‑1 (MQ=60)
                                                                         CTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGG                      <  SRR4302540.8717999/94‑1 (MQ=60)
                                                                         CTCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGG                      >  SRR4302540.9176485/1‑94 (MQ=60)
                                                                          TCCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGT                     <  SRR4302540.608769/94‑1 (MQ=60)
                                                                           CCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTC                    >  SRR4302540.6606390/1‑94 (MQ=60)
                                                                           CCCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTC                    <  SRR4302540.8894807/94‑1 (MQ=60)
                                                                            CCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAGAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATCGAGAAGTCGGGGTCA                   >  SRR4302540.5977656/1‑94 (MQ=60)
                                                                            CCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCA                   >  SRR4302540.8526568/1‑94 (MQ=60)
                                                                            CCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCA                   >  SRR4302540.6864536/1‑94 (MQ=60)
                                                                            CCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCA                   >  SRR4302540.5977655/1‑94 (MQ=60)
                                                                            CCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCA                   >  SRR4302540.6969224/1‑94 (MQ=60)
                                                                            CCCCTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCA                   >  SRR4302540.2762801/1‑94 (MQ=60)
                                                                               CTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAG                >  SRR4302540.9762051/1‑94 (MQ=60)
                                                                               CTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAG                >  SRR4302540.3550379/1‑94 (MQ=60)
                                                                               CTTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAG                >  SRR4302540.9762052/1‑94 (MQ=60)
                                                                                TTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAGA               <  SRR4302540.3864534/94‑1 (MQ=60)
                                                                                TTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAGA               <  SRR4302540.9865753/94‑1 (MQ=60)
                                                                                TTCGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAGA               >  SRR4302540.3035517/1‑94 (MQ=60)
                                                                                  CGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAGATC             <  SRR4302540.10086246/94‑1 (MQ=60)
                                                                                  CGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAGATC             <  SRR4302540.9503103/94‑1 (MQ=60)
                                                                                  CGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAGATC             <  SRR4302540.2734738/94‑1 (MQ=60)
                                                                                  CGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAGATC             <  SRR4302540.5477985/94‑1 (MQ=60)
                                                                                  CGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAGATC             >  SRR4302540.5701354/1‑94 (MQ=60)
                                                                                  CGAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAGATC             >  SRR4302540.5832908/1‑94 (MQ=60)
                                                                                   GAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAGATCG            >  SRR4302540.149359/1‑94 (MQ=60)
                                                                                   GAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAGATCG            <  SRR4302540.2325757/94‑1 (MQ=60)
                                                                                   GAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAGATCG            >  SRR4302540.3112754/1‑94 (MQ=60)
                                                                                   GAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAGATCG            >  SRR4302540.6550787/1‑94 (MQ=60)
                                                                                   GAATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAGATCG            >  SRR4302540.7385205/1‑94 (MQ=60)
                                                                                     ATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAGATCGAC          <  SRR4302540.6989489/94‑1 (MQ=60)
                                                                                     ATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAGATCGAC          >  SRR4302540.8510029/1‑94 (MQ=60)
                                                                                     ATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAGATCGAC          >  SRR4302540.5903752/1‑94 (MQ=60)
                                                                                     ATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAGATCGAC          >  SRR4302540.8510028/1‑94 (MQ=60)
                                                                                     ATAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAGATCGAC          >  SRR4302540.3609154/1‑94 (MQ=60)
                                                                                     ATAGCCTACGCCAGCACACAAAAAGTGTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAGATCGAC          <  SRR4302540.6989490/94‑1 (MQ=60)
                                                                                      TAGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAGATCGACC         <  SRR4302540.6373046/94‑1 (MQ=60)
                                                                                       AGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAGATCGACCC        <  SRR4302540.8551670/94‑1 (MQ=60)
                                                                                       AGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAGATCGACCC        <  SRR4302540.901523/94‑1 (MQ=60)
                                                                                       AGCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAGATCGACAC        >  SRR4302540.7422905/1‑94 (MQ=60)
                                                                                        GCCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAGATCGACCCC       >  SRR4302540.1559356/1‑94 (MQ=60)
                                                                                         CCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAGATCGACCCCT      >  SRR4302540.6051331/1‑94 (MQ=60)
                                                                                         CCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAGATCGACCCCT      <  SRR4302540.2017664/94‑1 (MQ=60)
                                                                                         CCTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAGATCGACCCCT      <  SRR4302540.1497082/94‑1 (MQ=60)
                                                                                          CTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAGATCGACCCCTT     >  SRR4302540.5299012/1‑94 (MQ=60)
                                                                                          CTACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAGATCGACCCCTT     <  SRR4302540.159847/94‑1 (MQ=60)
                                                                                           TACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAGATCGACCCCTTT    <  SRR4302540.1608710/94‑1 (MQ=60)
                                                                                            ACGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAGATCGACCCCTTTT   >  SRR4302540.6734126/1‑94 (MQ=60)
                                                                                             CGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAGATCGACCCCTTTTT  <  SRR4302540.7190872/94‑1 (MQ=60)
                                                                                             |                                                                                             
ATAAATAGTTACACGTTGGTGAACACCCAAACGTCTTCAATTGTTGAGGTTCACACGACAAAGTCCGGCAAACCTACCCCTTCGAATAGCCTATGCCAGCACACAAAAAGTTTTGGGAAAAAAACTTTCTTGTAACGTGGGGTCGCAGGATTATAGAGAAGTCGGGGTCAAAGATCGACCCCTTTTT  >  NC_000913/3376301‑3376487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑