Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,421,120	Δ1 bp	noncoding (60/121 nt)	rrfF ←	5S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,421,119	0	G	.	93.1%	317.8 / 24.6	130	noncoding (61/121 nt)	rrfF	5S ribosomal RNA
Reads supporting (aligned to +/- strand): ref base G (8/1); new base . (55/66); total (63/67)
Fisher's exact test for biased strand distribution p-value = 1.48e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.00e-01

Only 100 of 156 total aligned reads displayed.

CAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAA  >  NC_000913/3421027‑3421207

cAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACGCCACACTACCATCGGCGCTAcggcg                                                                                          >  1:5335923/1‑93 (MQ=255)

cAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTAcggcg                                                                                          >  2:9776494/1‑93 (MQ=255)

cAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTAcggcg                                                                                          <  1:4298269/93‑1 (MQ=40)

 aGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTAcggcgt                                                                                         >  2:2311197/1‑92 (MQ=39)

 aGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTAcggcgt                                                                                         >  2:1740757/1‑92 (MQ=255)

 aGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTAcggcgt                                                                                         >  1:9801217/1‑92 (MQ=255)

 aGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACAATCGGCGCTAcggcgt                                                                                         >  1:6795445/1‑92 (MQ=255)

  gTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTAcggcgtt                                                                                        >  1:1018005/1‑91 (MQ=255)

  gTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTAcggcgtt                                                                                        >  1:14738/1‑91 (MQ=38)

  gTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTAcggcgtt                                                                                        >  1:7360673/1‑91 (MQ=255)

  gTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTAcggcgtt                                                                                        >  1:5841170/1‑91 (MQ=255)

  gTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTAcggcgtt                                                                                        >  1:408343/1‑91 (MQ=255)

   tCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGttt                                                                                       >  1:6904520/1‑93 (MQ=255)

   tCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGttt                                                                                       >  1:6538421/1‑93 (MQ=255)

   tCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGttt                                                                                       >  1:6003201/1‑93 (MQ=255)

   tCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGttt                                                                                       >  1:5666551/1‑93 (MQ=255)

   tCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGttt                                                                                       >  1:2232928/1‑93 (MQ=255)

   tCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGCCGCTACGGCGttt                                                                                       >  1:6904217/1‑93 (MQ=255)

    cTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGtttc                                                                                      >  1:4480314/1‑92 (MQ=255)

     ttCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCa                                                                                    >  1:6332351/1‑93 (MQ=19)

     ttCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCa                                                                                    >  1:5803322/1‑93 (MQ=255)

     ttCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCa                                                                                    <  2:1816539/93‑1 (MQ=19)

     ttCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCACATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCa                                                                                    <  1:8617422/93‑1 (MQ=255)

      tCCGACTGAGCGTTTTGTTTTATTTGAGGTCTGGCAGTTCCCTGCACTCGCATGGGGAGACCCTGCACTACCATCGGCGCTACGGCGGttcac                                                                                    <  2:7586337/93‑4 (MQ=14)

      tCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCAc                                                                                   >  2:5063078/1‑93 (MQ=19)

         gACTGAGCTTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTc                                                                                <  1:7493139/93‑1 (MQ=9)

         gACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTc                                                                                >  1:1220355/1‑93 (MQ=16)

         gACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTc                                                                                <  1:9970611/93‑1 (MQ=9)

         gACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTc                                                                                <  2:6063585/93‑1 (MQ=9)

         gACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTc                                                                                >  2:8563436/1‑93 (MQ=9)

         gACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTc                                                                                >  2:4037124/1‑93 (MQ=16)

         gACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTc                                                                                >  2:9845214/1‑93 (MQ=16)

         gACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTc                                                                                <  2:4942604/93‑1 (MQ=9)

          aCTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCt                                                                               >  1:2251819/1‑93 (MQ=16)

          aCTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCt                                                                               >  2:3142740/1‑93 (MQ=16)

            tGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGATACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGa                                                                             >  1:4995806/1‑93 (MQ=9)

            tGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGa                                                                             <  1:121410/93‑1 (MQ=9)

            tGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGa                                                                             >  1:8461728/1‑93 (MQ=16)

            tGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGa                                                                             <  1:7768805/93‑1 (MQ=9)

             gAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAg                                                                            <  2:3023673/93‑1 (MQ=9)

             gAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAg                                                                            >  2:2384422/1‑93 (MQ=12)

             gAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAg                                                                            >  2:8873827/1‑93 (MQ=16)

              aGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGt                                                                           <  2:9342417/93‑1 (MQ=9)

              aGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGt                                                                           >  2:1246973/1‑93 (MQ=16)

               gCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGtt                                                                          >  2:8492409/1‑93 (MQ=16)

               gCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGtt                                                                          >  2:7326468/1‑93 (MQ=16)

                ccTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTc                                                                         <  2:9893484/93‑1 (MQ=9)

                ccTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTc                                                                         >  2:4291264/1‑93 (MQ=12)

                 cTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCg                                                                        >  2:7945925/1‑93 (MQ=12)

                 cTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCg                                                                        >  1:4930513/1‑93 (MQ=12)

                 cTTTTGTTTTAATTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTct                                                                        <  2:8961692/93‑2 (MQ=255)

                  ttttGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCgg                                                                       >  2:4234899/1‑93 (MQ=12)

                   tttGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGc                                                                      <  1:3694507/93‑1 (MQ=9)

                    ttGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCa                                                                     <  1:9939067/93‑1 (MQ=0)

                    ttGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCa                                                                     <  1:8154290/93‑1 (MQ=0)

                    ttGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCa                                                                     <  2:5559390/93‑1 (MQ=0)

                                                                   cccACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCttt                      <  2:7130761/93‑1 (MQ=0)

                                                                    ccACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCtttt                     >  2:1304549/1‑93 (MQ=9)

                                                                    ccACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCtttt                     >  2:5890376/1‑93 (MQ=9)

                                                                     cacaCTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTc                    <  1:1362058/93‑1 (MQ=12)

                                                                     cacaCTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTc                    <  2:5335922/93‑1 (MQ=12)

                                                                     cacaCTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTc                    <  2:231649/93‑1 (MQ=16)

                                                                      ccacTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCt                   <  1:2952884/92‑1 (MQ=16)

                                                                      ccacTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCt                   <  1:4521091/92‑1 (MQ=16)

                                                                      acacTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCt                   >  1:3283593/1‑93 (MQ=16)

                                                                      acacTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCt                   <  1:3952533/93‑1 (MQ=19)

                                                                      acacTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCt                   <  2:5859323/93‑1 (MQ=19)

                                                                       cacTACCATCGGCGCTACGGC‑GTTTTACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTa                  <  1:6819821/93‑1 (MQ=255)

                                                                       cacTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTa                  >  2:7471989/1‑93 (MQ=19)

                                                                       cacTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTa                  <  1:4386481/93‑1 (MQ=19)

                                                                        ccTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTaa                 <  2:5908627/92‑1 (MQ=255)

                                                                        acTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTaa                 <  1:6067731/93‑1 (MQ=255)

                                                                        acTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTaa                 <  2:5176320/93‑1 (MQ=255)

                                                                         cTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAAt                <  1:1097493/93‑1 (MQ=255)

                                                                          tACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTTTAATc               >  2:1418355/1‑93 (MQ=9)

                                                                          tACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATc               <  2:1870197/93‑1 (MQ=255)

                                                                          tACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATc               >  2:5617640/1‑93 (MQ=255)

                                                                          tACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATc               <  1:9328903/93‑1 (MQ=255)

                                                                          tACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATc               >  1:623496/1‑93 (MQ=255)

                                                                           aCCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCt              >  2:4719830/1‑93 (MQ=255)

                                                                           aCCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCt              <  1:9299649/93‑1 (MQ=255)

                                                                           aCCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCt              <  2:6292051/93‑1 (MQ=255)

                                                                           aCCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCt              <  1:8899527/93‑1 (MQ=255)

                                                                            ccATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTg             <  1:7349090/93‑1 (MQ=255)

                                                                             cATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGc            >  2:7080609/1‑93 (MQ=255)

                                                                             cATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGCGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGc            <  1:1816314/93‑1 (MQ=255)

                                                                              aTCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATATGcc           >  2:4636585/1‑93 (MQ=255)

                                                                                 ggCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGaa        <  1:1740757/93‑1 (MQ=255)

                                                                                  gcgcTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTTATCTGCCGAAc       >  1:8932381/1‑93 (MQ=255)

                                                                                  gcgcTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAAc       <  1:5722008/93‑1 (MQ=255)

                                                                                  gcgcTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAAc       >  1:2732889/1‑93 (MQ=255)

                                                                                   cgcTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACt      <  2:6478062/93‑1 (MQ=255)

                                                                                    gcTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACtt     <  1:8506651/93‑1 (MQ=255)

                                                                                    gcTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACtt     >  1:206595/1‑93 (MQ=255)

                                                                                     cTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACttt    <  1:3321489/93‑1 (MQ=255)

                                                                                     cTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACttt    <  2:1632086/93‑1 (MQ=255)

                                                                                      tACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTa   <  1:8077220/93‑1 (MQ=255)

                                                                                      tACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTa   <  2:9386074/93‑1 (MQ=255)

                                                                                       aCGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTaa  <  1:8317498/93‑1 (MQ=255)

                                                                                       aCGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTaa  <  2:6887842/93‑1 (MQ=255)

CAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAA  >  NC_000913/3421027‑3421207

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAA  >  NC_000913/3421027‑3421213

CAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGT                                                                                               >  SRR4302540.9915972/1‑94 (MQ=36)

CAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGT                                                                                               <  SRR4302540.4345050/94‑1 (MQ=36)

CAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACAATCGGCGCTACGGCGT                                                                                               >  SRR4302540.6870603/1‑94 (MQ=35)

 AGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTT                                                                                              >  SRR4302540.412616/1‑94 (MQ=34)

 AGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTT                                                                                              >  SRR4302540.1028328/1‑94 (MQ=34)

 AGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTT                                                                                              >  SRR4302540.7335770/1‑94 (MQ=34)

 AGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTT                                                                                              >  SRR4302540.14904/1‑94 (MQ=34)

 AGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTT                                                                                              >  SRR4302540.5905763/1‑94 (MQ=34)

 AGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTT                                                                                              >  SRR4302540.8082576/1‑94 (MQ=34)

 AGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTT                                                                                              >  SRR4302540.7442752/1‑94 (MQ=34)

  GTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTT                                                                                             >  SRR4302540.6069742/1‑94 (MQ=31)

  GTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTT                                                                                             >  SRR4302540.6610980/1‑94 (MQ=31)

  GTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTT                                                                                             >  SRR4302540.6069740/1‑94 (MQ=31)

  GTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTT                                                                                             >  SRR4302540.2256960/1‑94 (MQ=31)

  GTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTT                                                                                             >  SRR4302540.6981280/1‑94 (MQ=31)

  GTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTT                                                                                             >  SRR4302540.5728956/1‑94 (MQ=31)

  GTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGCCGCTACGGCGTTT                                                                                             >  SRR4302540.6980972/1‑94 (MQ=29)

   TCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTC                                                                                           >  SRR4302540.4528900/1‑94 (MQ=28)

    CTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCA                                                                                          >  SRR4302540.5867443/1‑94 (MQ=22)

    CTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCA                                                                                          >  SRR4302540.6402867/1‑94 (MQ=22)

     TTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCACATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCAC                                                                                         <  SRR4302540.8717534/94‑1 (MQ=50)

       CCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTT                                                                                       <  SRR4302540.663666/94‑1 (MQ=7)

        CGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTC                                                                                      >  SRR4302540.1232842/1‑94 (MQ=4)

        CGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTC                                                                                      <  SRR4302540.4025712/94‑1 (MQ=4)

         GACTGAGCTTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCT                                                                                     <  SRR4302540.7576815/94‑1 (MQ=4)

         GACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCT                                                                                     >  SRR4302540.2275990/1‑94 (MQ=4)

         GACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCT                                                                                     <  SRR4302540.10086922/94‑1 (MQ=4)

           CTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGATACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGA                                                                                   >  SRR4302540.5049889/1‑94 (MQ=4)

           CTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGA                                                                                   >  SRR4302540.8559982/1‑94 (MQ=4)

            TGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGG‑TTCACTTCTGAG                                                                                  >  SRR4302540.10078050/1‑94 (MQ=20)

            TGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAG                                                                                  <  SRR4302540.7859631/94‑1 (MQ=4)

            TGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAG                                                                                  <  SRR4302540.122710/94‑1 (MQ=4)

            TGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAG                                                                                  >  SRR4302540.4208220/1‑94 (MQ=4)

            TGAGCCTTTTGTTTTATTTGATGGCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGCCGG‑TTCACTTCTGAT                                                                                  >  SRR4302540.4208221/1‑94 (MQ=4)

              AGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTT                                                                                <  SRR4302540.6948664/94‑1 (MQ=4)

                CCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCG                                                                              <  SRR4302540.2037163/94‑1 (MQ=4)

                CCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCG                                                                              >  SRR4302540.4983665/1‑94 (MQ=4)

                 CTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGG                                                                             >  SRR4302540.7548363/1‑94 (MQ=4)

                 CTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGG                                                                             >  SRR4302540.1222537/1‑94 (MQ=4)

                   TTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCA                                                                           >  SRR4302540.1739692/1‑94 (MQ=0)

                   TTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCA                                                                           <  SRR4302540.3734852/94‑1 (MQ=0)

                    TTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCAT                                                                          <  SRR4302540.8248967/94‑1 (MQ=0)

                    TTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCAT                                                                          >  SRR4302540.5084048/1‑94 (MQ=0)

                                                                    CCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTC                          >  SRR4302540.1866308/1‑94 (MQ=0)

                                                                    CCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTC                          <  SRR4302540.6581863/94‑1 (MQ=0)

                                                                     CACACTACCATCGGCGCTACGGCGG‑TTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACAACGGCGCTACTGCGGCCAGACAAATTCTTTTCT                         >  SRR4302540.3319483/1‑94 (MQ=17)

                                                                     CACACTACCATCGGCGCTACGGC‑TTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCT                         >  SRR4302540.1827406/1‑94 (MQ=1)

                                                                     CACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCT                         >  SRR4302540.3319484/1‑94 (MQ=1)

                                                                     CACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCT                         <  SRR4302540.1376447/94‑1 (MQ=1)

                                                                     CACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCT                         >  SRR4302540.8444990/1‑94 (MQ=1)

                                                                     CACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCT                         <  SRR4302540.5658259/94‑1 (MQ=1)

                                                                     CACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCT                         >  SRR4302540.5425488/1‑94 (MQ=1)

                                                                      CCACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTA                        <  SRR4302540.4570054/94‑1 (MQ=4)

                                                                      CCACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTA                        <  SRR4302540.2985396/94‑1 (MQ=4)

                                                                      ACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTA                        <  SRR4302540.3995184/94‑1 (MQ=4)

                                                                      ACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTA                        >  SRR4302540.5159800/1‑94 (MQ=4)

                                                                      ACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTA                        >  SRR4302540.8193247/1‑94 (MQ=4)

                                                                       CACTACCATCGGCGCTACGGC‑GTTTTACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAA                       <  SRR4302540.6895315/94‑1 (MQ=6)

                                                                       CACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAA                       <  SRR4302540.4434099/94‑1 (MQ=6)

                                                                        ACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAAT                      <  SRR4302540.6135194/94‑1 (MQ=9)

                                                                         CTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATC                     >  SRR4302540.630134/1‑94 (MQ=11)

                                                                         CTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATC                     <  SRR4302540.1108791/94‑1 (MQ=11)

                                                                          TACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCT                    <  SRR4302540.9440149/94‑1 (MQ=20)

                                                                          TACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCT                    <  SRR4302540.3067988/94‑1 (MQ=20)

                                                                           CCCATCGGCGCTAC‑GCCGTTGCACTTGTGAGGTCGTCATGTGGGCAGGTGGGACCACCGCGCTACTGCCGCCACACAAATTCTTTTCTAATCTG                   <  SRR4302540.9002551/94‑1 (MQ=15)

                                                                           ACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTG                   <  SRR4302540.5837026/94‑1 (MQ=22)

                                                                           ACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTG                   <  SRR4302540.9410741/94‑1 (MQ=22)

                                                                           ACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTG                   >  SRR4302540.1270462/1‑94 (MQ=22)

                                                                           ACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTG                   <  SRR4302540.3725091/94‑1 (MQ=22)

                                                                           ACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTG                   <  SRR4302540.9002550/94‑1 (MQ=22)

                                                                            CCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGC                  <  SRR4302540.7431080/94‑1 (MQ=24)

                                                                             CATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCC                 >  SRR4302540.5308863/1‑94 (MQ=27)

                                                                             CATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGCGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCC                 <  SRR4302540.1836153/94‑1 (MQ=26)

                                                                              ATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCG                >  SRR4302540.2139400/1‑94 (MQ=29)

                                                                               TCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGA               >  SRR4302540.10032944/1‑94 (MQ=32)

                                                                                CGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCATGTGGGACTACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAA              <  SRR4302540.4483105/94‑1 (MQ=31)

                                                                                 GGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTTATCTGCCGAAC             >  SRR4302540.9036699/1‑94 (MQ=23)

                                                                                 GGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAAC             >  SRR4302540.2762382/1‑94 (MQ=37)

                                                                                 GGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAAC             >  SRR4302540.845488/1‑94 (MQ=37)

                                                                                 GGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAAC             <  SRR4302540.6253855/94‑1 (MQ=37)

                                                                                 GGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAAC             >  SRR4302540.4780010/1‑94 (MQ=37)

                                                                                 GGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAAC             <  SRR4302540.1759589/94‑1 (MQ=37)

                                                                                  GCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACT            <  SRR4302540.5784997/94‑1 (MQ=39)

                                                                                   CGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCTCCAGACAAATTCTTTTCTAATCTGCCGAACTT           >  SRR4302540.8607573/1‑94 (MQ=40)

                                                                                   CGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTT           >  SRR4302540.208621/1‑94 (MQ=41)

                                                                                   CGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTT           >  SRR4302540.964300/1‑94 (MQ=41)

                                                                                    GCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTT          <  SRR4302540.8605509/94‑1 (MQ=44)

                                                                                     CTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTA         <  SRR4302540.3357927/94‑1 (MQ=46)

                                                                                      TACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAA        <  SRR4302540.8170963/94‑1 (MQ=52)

                                                                                      TACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAA        >  SRR4302540.2638577/1‑94 (MQ=52)

                                                                                      TACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAA        >  SRR4302540.3527872/1‑94 (MQ=52)

                                                                                       ACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAAC       <  SRR4302540.8414054/94‑1 (MQ=57)

                                                                                       ACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAAC       <  SRR4302540.9374641/94‑1 (MQ=57)

                                                                                        CGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACC      >  SRR4302540.46977/1‑94 (MQ=60)

                                                                                         GGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCT     <  SRR4302540.9961100/94‑1 (MQ=60)

                                                                                         GGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCT     <  SRR4302540.6981547/94‑1 (MQ=60)

                                                                                         GGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCT     <  SRR4302540.294246/94‑1 (MQ=60)

                                                                                          cgcGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCT     <  SRR4302540.294245/91‑1 (MQ=60)

                                                                                           gcGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTA    <  SRR4302540.346255/92‑1 (MQ=60)

                                                                                            CGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAA   >  SRR4302540.9009095/1‑94 (MQ=60)

                                                                                             GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAA  >  SRR4302540.1408866/1‑94 (MQ=60)

                                                                                             GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAA  >  SRR4302540.1741725/1‑94 (MQ=60)

                                                                                             GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAA  <  SRR4302540.2477976/94‑1 (MQ=60)

                                                                                             GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAA  >  SRR4302540.571942/1‑94 (MQ=60)

                                                                                             GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAA  >  SRR4302540.5324476/1‑94 (MQ=60)

                                                                                             GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAA  >  SRR4302540.3961878/1‑94 (MQ=60)

                                                                                             GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAA  >  SRR4302540.435029/1‑94 (MQ=60)

CAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAA  >  NC_000913/3421027‑3421213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`