Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
1548 |
369.5 |
20095359 |
96.9% |
19472402 |
93.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,941,531 |
T→C |
noncoding (206/2906 nt) |
rrlC → |
23S ribosomal RNA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,941,531 | 0 | T | C | 96.3%
| 108.8
/ ‑2.6
| 55 | noncoding (206/2906 nt) | rrlC | 23S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base T (0/2); new base C (24/28); total (25/30) |
| Fisher's exact test for biased strand distribution p-value = 4.97e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01 |
TGAAACATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAG > NC_000913/3941518‑3941620
|
tGAAACATCTAAGCGCCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTa < 2:3744981/93‑1 (MQ=9)
tGAAACATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGGCCAGAGCCTGAATCAGTa > 2:1711967/1‑93 (MQ=9)
tGAAACATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTa < 1:9358890/93‑1 (MQ=14)
tGAAACATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTa < 2:9437709/93‑1 (MQ=14)
tGAAACATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTa > 1:3393652/1‑93 (MQ=14)
tGAAACATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTa < 1:7663341/93‑1 (MQ=14)
tGAAACATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTa > 1:393115/1‑93 (MQ=14)
tGAAACATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTa < 2:185508/93‑1 (MQ=14)
tGAAACATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTa > 2:5554860/1‑93 (MQ=14)
tGAAACATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACCGGGAGCAGCCCAGAGCCTGAATCAGTa > 1:9842352/1‑93 (MQ=9)
gAAACATCTAAGCACCCGGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAt < 2:6130983/93‑1 (MQ=12)
gAAACATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGCACGGGGAGAAGCACAGAGCCTGAATCAGTAt > 2:4236103/1‑93 (MQ=11)
gAAACATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAt > 1:9306383/1‑93 (MQ=17)
gAAACATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAt < 1:8189650/93‑1 (MQ=17)
gAAACATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAt > 1:7333960/1‑93 (MQ=17)
gAAACATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAt > 2:691338/1‑93 (MQ=17)
gAAACATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAt < 2:7145081/93‑1 (MQ=17)
aaaaCATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAt < 1:2571667/92‑1 (MQ=17)
aaaCATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtg < 2:6094417/93‑1 (MQ=33)
aaaCATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtg < 1:7357382/93‑1 (MQ=18)
aaaCATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtg > 1:1219914/1‑93 (MQ=18)
aaCATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtgt > 2:563005/1‑93 (MQ=18)
aaCATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtgt < 1:9990081/93‑1 (MQ=18)
aaCATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtgt < 1:8235952/93‑1 (MQ=18)
aaCATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtgt < 1:872930/93‑1 (MQ=18)
aCATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtgtg < 2:4950668/93‑1 (MQ=18)
aCATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtgtg < 2:9933839/93‑1 (MQ=18)
aCATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtgtg < 1:2663443/93‑1 (MQ=18)
cATCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtgtgt < 2:8053289/93‑1 (MQ=21)
cATATAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtgtgg > 1:5573464/1‑92 (MQ=255)
aTCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtgtgtg > 1:1531550/1‑93 (MQ=18)
aTCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtgtgtg > 1:8121239/1‑93 (MQ=18)
aTCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtgtgtg > 1:6357541/1‑93 (MQ=18)
aTCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtgtgtg > 2:3826444/1‑93 (MQ=18)
aTCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAtgtgtg < 1:5854471/93‑1 (MQ=21)
aTCTAAGAACCCCGAGGAAAAGAAATCAACCGAGATTCACCCAGTAGCGGCGAGCGAACCGGGAGCAGCCCAGAGCCTGAATCAGTAtgtgtg > 2:1731803/1‑93 (MQ=255)
tCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGGGCGAACGGGGGGCGGACCAGAGCCTGAATCAGGAtgttggt > 2:1710834/1‑89 (MQ=11)
tCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATgtgtgt < 1:5116697/93‑1 (MQ=18)
tCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATgtgtgt < 1:2925487/93‑1 (MQ=21)
tCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATgtgtgt > 1:2987223/1‑93 (MQ=18)
tCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATgtgtgt < 1:3986643/93‑1 (MQ=18)
tCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATgtgtgt > 1:9115165/1‑93 (MQ=18)
tCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATgtgtgt > 1:7653535/1‑93 (MQ=18)
tCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATgtgtgt < 1:9042169/93‑1 (MQ=21)
tCTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCGGAATCAGTATgggtgt > 1:796613/1‑93 (MQ=255)
cTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGtt < 2:685984/93‑1 (MQ=21)
cTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGtt > 1:2782277/1‑93 (MQ=18)
cTAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGtt < 1:9712793/93‑1 (MQ=21)
tAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTa < 2:5148387/93‑1 (MQ=18)
tAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTa < 1:3411076/93‑1 (MQ=18)
tAAGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTa < 2:810304/93‑1 (MQ=21)
caaGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTa < 2:8636968/92‑1 (MQ=18)
aaGTACCCCGAGGAACAGACATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTAAATGTGttcg < 2:8987800/93‑3 (MQ=11)
aaGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAg < 1:7785886/93‑1 (MQ=21)
aaGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAg > 2:6603674/1‑93 (MQ=18)
aaGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAg > 2:6603675/1‑93 (MQ=18)
aaGCACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAg < 1:8113781/93‑1 (MQ=18)
|
TGAAACATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTATGTGTGTTAG > NC_000913/3941518‑3941620
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
