Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,429,527 Δ1 bp coding (40/510 nt) yrdD ← putative DNA topoisomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,429,5260C.100.0% 993.8 / NA 215coding (41/510 nt)yrdDputative DNA topoisomerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (100/115);  total (100/115)

Only 100 of 241 total aligned reads displayed.

CGATATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATCCGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTG  >  NC_000913/3429434‑3429614
                                                                                            |                                                                                        
cGATATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATc                                                                                          <  1:3264094/93‑1 (MQ=255)
cGATATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATc                                                                                          <  2:1478206/93‑1 (MQ=255)
 gATATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATcg                                                                                         >  1:1203584/1‑92 (MQ=255)
  atatGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATcga                                                                                        <  2:5622688/93‑3 (MQ=255)
  atatGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATcga                                                                                        >  1:5593084/1‑91 (MQ=255)
   tatGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATcgaa                                                                                       <  2:759466/93‑4 (MQ=255)
   tatGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATcgaa                                                                                       <  1:2478988/93‑4 (MQ=255)
   tatGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATcgaa                                                                                       <  1:6260122/93‑4 (MQ=255)
    atGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATcgaat                                                                                      <  1:2775906/93‑5 (MQ=255)
     tGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATa                                                                                    >  2:1933306/1‑93 (MQ=255)
        ccATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAAcc                                                                                 <  1:5671410/93‑1 (MQ=255)
        ccATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAAcc                                                                                 <  2:3441498/93‑1 (MQ=255)
          aTCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAg                                                                               <  1:3953518/93‑1 (MQ=255)
           tCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGt                                                                              <  1:616386/93‑1 (MQ=255)
           tCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGt                                                                              <  1:4158378/93‑1 (MQ=255)
           tCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGt                                                                              >  2:5172903/1‑93 (MQ=255)
           tCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGt                                                                              >  1:2922146/1‑93 (MQ=255)
             cGCTGAAGATTTCAGAGGACGGACGTAGTGACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGtta                                                                            >  2:849035/1‑92 (MQ=255)
             cGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTc                                                                            <  2:5951526/93‑1 (MQ=255)
             cGCTGAAGATTCCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGCCCGTGTTTCCCGGAT‑CGAATAACCAGTTc                                                                            <  2:2417733/93‑1 (MQ=255)
               cTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATGACCAGTTCAg                                                                          >  2:5859622/1‑93 (MQ=255)
               cTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAg                                                                          >  1:4255384/1‑93 (MQ=255)
               cTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAg                                                                          >  2:5983907/1‑93 (MQ=255)
               cTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAg                                                                          >  1:258805/1‑93 (MQ=255)
                tGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGc                                                                         >  2:4670839/1‑93 (MQ=255)
                 gAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGcc                                                                        >  1:1766252/1‑93 (MQ=255)
                 gAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGcc                                                                        <  1:661926/93‑1 (MQ=255)
                 gAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGcc                                                                        <  2:3413437/93‑1 (MQ=255)
                   aGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGcccc                                                                      <  1:3093934/93‑1 (MQ=255)
                    gATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCg                                                                     <  2:3781988/93‑1 (MQ=255)
                      tttCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCa                                                                   <  2:2238940/93‑1 (MQ=255)
                       ttCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCAc                                                                  >  1:4456892/1‑93 (MQ=255)
                       ttCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCAc                                                                  <  2:3731188/93‑1 (MQ=255)
                         cAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACtt                                                                >  1:705819/1‑93 (MQ=255)
                            agGACGGACGTAATCACCCGGCGGATTCTGGTAACAACCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTgg                                                             <  2:1757991/93‑1 (MQ=255)
                             ggacggacGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTggg                                                            <  1:3173167/93‑1 (MQ=255)
                                cggacgTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAg                                                         <  2:3950074/93‑1 (MQ=255)
                                  gacgTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGa                                                       >  2:3135355/1‑93 (MQ=255)
                                  gacgTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGa                                                       <  1:3421435/93‑1 (MQ=255)
                                  gacgTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGa                                                       >  2:2280684/1‑93 (MQ=255)
                                   acgTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGAc                                                      <  2:2347926/93‑1 (MQ=255)
                                      tAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCa                                                   <  1:3605095/93‑1 (MQ=255)
                                      tAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCa                                                   >  1:5205316/1‑93 (MQ=255)
                                         tCACAGGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCatta                                                <  2:206871/93‑1 (MQ=255)
                                          cacaCGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCattat                                               <  1:1840126/93‑1 (MQ=255)
                                           acacGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCattatt                                              <  1:2528515/93‑1 (MQ=255)
                                           acacGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCattatt                                              >  2:487136/1‑93 (MQ=255)
                                           acacGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCattatt                                              <  1:2232632/93‑1 (MQ=255)
                                            cacGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCAttatta                                             <  1:4597661/93‑1 (MQ=255)
                                            cacGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCAttatta                                             <  2:2567696/93‑1 (MQ=255)
                                              cGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACg                                           >  1:3741853/1‑93 (MQ=255)
                                               gCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGc                                          >  1:1568486/1‑93 (MQ=255)
                                                 cGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCAc                                        >  1:102023/1‑93 (MQ=255)
                                                 cGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCAc                                        >  1:5952992/1‑93 (MQ=255)
                                                  ggATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCAcc                                       <  2:4566208/93‑1 (MQ=255)
                                                  ggATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCAcc                                       <  2:2792016/93‑1 (MQ=255)
                                                  ggATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCAcc                                       >  1:474399/1‑93 (MQ=255)
                                                  gaaTACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCAcc                                       <  1:4391386/91‑1 (MQ=255)
                                                   gATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCg                                      <  1:213681/93‑1 (MQ=255)
                                                     tACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTg                                    >  2:4216521/1‑93 (MQ=255)
                                                     tACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTg                                    >  2:1001483/1‑93 (MQ=255)
                                                       cTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGaa                                  <  1:4544132/93‑1 (MQ=255)
                                                        tgtgAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAAc                                 <  2:6046156/93‑1 (MQ=255)
                                                        tgtgAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAAc                                 >  1:4472213/1‑93 (MQ=255)
                                                        tgtgAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAAc                                 >  2:2591756/1‑93 (MQ=255)
                                                        tgtgAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAAc                                 >  2:5429412/1‑93 (MQ=255)
                                                        tgtgAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAAc                                 <  1:5365904/93‑1 (MQ=255)
                                                         gtgAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACa                                <  1:1285584/93‑1 (MQ=255)
                                                         gtgAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACa                                <  1:400883/93‑1 (MQ=255)
                                                          tgAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAg                               >  1:4722592/1‑93 (MQ=255)
                                                          tgAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAg                               >  1:2207577/1‑93 (MQ=255)
                                                           gAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGt                              <  2:1892574/93‑1 (MQ=255)
                                                             gCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGc                            >  1:1661519/1‑93 (MQ=255)
                                                             gCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGc                            <  1:191891/93‑1 (MQ=255)
                                                              cATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCt                           <  2:5378790/93‑1 (MQ=255)
                                                                tCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGa                         <  1:1339267/93‑1 (MQ=255)
                                                                tCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGa                         <  2:1294934/93‑1 (MQ=255)
                                                                 ccAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGAt                        <  1:3972629/93‑1 (MQ=255)
                                                                  cAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGAtt                       <  1:1610875/93‑1 (MQ=255)
                                                                  cAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGAtt                       <  1:196387/93‑1 (MQ=255)
                                                                        aCGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCa                 <  2:1438917/93‑1 (MQ=255)
                                                                            gccGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATaac             <  1:3557899/92‑1 (MQ=255)
                                                                            aCCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATaac             <  2:2679100/93‑1 (MQ=255)
                                                                              cGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACCCACCTTGAACAGTGCTGATTTCGCCATaaaaa           >  1:2321950/1‑93 (MQ=255)
                                                                               gtgtTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATaacaac          >  1:3802696/1‑93 (MQ=255)
                                                                               gtgtTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATaacaac          <  1:2266639/93‑1 (MQ=255)
                                                                                tgtTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACt         >  2:297686/1‑93 (MQ=255)
                                                                                 gtTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACtt        <  1:5786199/93‑1 (MQ=255)
                                                                                  tttCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTa       <  1:5464869/93‑1 (MQ=255)
                                                                                  tttCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTa       <  1:5983907/93‑1 (MQ=255)
                                                                                   ttCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTAt      <  2:6270651/93‑1 (MQ=255)
                                                                                    tCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAAc                                 >  2:3902450/1‑65 (MQ=255)
                                                                                    tCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAAc                                 <  1:3902450/65‑1 (MQ=255)
                                                                                    tCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATg     >  2:5687992/1‑93 (MQ=255)
                                                                                     cccGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGc    <  2:4478639/93‑1 (MQ=255)
                                                                                      ccGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCt   <  1:5137891/93‑1 (MQ=255)
                                                                                      ccGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCt   >  1:1180435/1‑93 (MQ=255)
                                                                                       cGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTg  <  1:5011953/93‑1 (MQ=255)
                                                                                       cGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTg  >  1:1887027/1‑93 (MQ=255)
                                                                                       cGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTg  >  1:6109468/1‑93 (MQ=255)
                                                                                            |                                                                                        
CGATATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATCCGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTG  >  NC_000913/3429434‑3429614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGATATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATCCGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAATTAA  >  NC_000913/3429434‑3429620
                                                                                             |                                                                                             
CGATATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATCG                                                                                               >  SRR4302533.810387/1‑94 (MQ=60)
CGATATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATCG                                                                                               <  SRR4302533.3284228/94‑1 (MQ=60)
CGATATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATCG                                                                                               >  SRR4302533.1210816/1‑94 (MQ=60)
CGATATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATCG                                                                                               >  SRR4302533.233926/1‑94 (MQ=60)
CGATATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATCG                                                                                               >  SRR4302533.4525590/1‑94 (MQ=60)
CGATATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATCG                                                                                               <  SRR4302533.4329785/94‑1 (MQ=60)
CGATATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATCG                                                                                               >  SRR4302533.1612908/1‑94 (MQ=60)
CGATATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGGTTCCCGGATCG                                                                                               >  SRR4302533.5638510/1‑94 (MQ=60)
CGATAGGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATCG                                                                                               <  SRR4302533.2476594/94‑1 (MQ=60)
   TATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAAT                                                                                           <  SRR4302533.2244226/94‑1 (MQ=60)
   TATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAAT                                                                                           <  SRR4302533.6297549/94‑1 (MQ=60)
   TATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAAT                                                                                           <  SRR4302533.5570103/94‑1 (MQ=60)
   TATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAAT                                                                                           <  SRR4302533.2494277/94‑1 (MQ=60)
    ATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATA                                                                                          <  SRR4302533.2792956/94‑1 (MQ=60)
      GTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAAC                                                                                        <  SRR4302533.3333407/94‑1 (MQ=60)
      GTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAAC                                                                                        >  SRR4302533.649715/1‑94 (MQ=60)
        CCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCA                                                                                      >  SRR4302533.3495933/1‑94 (MQ=60)
        CCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCA                                                                                      <  SRR4302533.5705661/94‑1 (MQ=60)
         CATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAG                                                                                     >  SRR4302533.361602/1‑94 (MQ=60)
          ATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGT                                                                                    >  SRR4302533.2940039/1‑94 (MQ=60)
          ATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGT                                                                                    <  SRR4302533.3977623/94‑1 (MQ=60)
           TCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTT                                                                                   <  SRR4302533.4183576/94‑1 (MQ=60)
           TCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTT                                                                                   <  SRR4302533.620005/94‑1 (MQ=60)
           TCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTT                                                                                   <  SRR4302533.4042839/94‑1 (MQ=60)
            CCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTC                                                                                  <  SRR4302533.3889226/94‑1 (MQ=60)
              GCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAG                                                                                >  SRR4302533.260378/1‑94 (MQ=60)
              GCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAG                                                                                >  SRR4302533.4281041/1‑94 (MQ=60)
              GCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAG                                                                                <  SRR4302533.1889883/94‑1 (MQ=60)
                TGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCC                                                                              >  SRR4302533.1777006/1‑94 (MQ=60)
                TGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCC                                                                              <  SRR4302533.378704/94‑1 (MQ=60)
                TGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCC                                                                              >  SRR4302533.4721211/1‑94 (MQ=60)
                 GAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCC                                                                             >  SRR4302533.2046011/1‑94 (MQ=60)
                 GAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCC                                                                             <  SRR4302533.665831/94‑1 (MQ=60)
                   AGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCG                                                                           <  SRR4302533.3112956/94‑1 (MQ=60)
                    TATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGC                                                                          <  SRR4302533.3508775/94‑1 (MQ=60)
                    GATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGC                                                                          >  SRR4302533.4649177/1‑94 (MQ=60)
                      TTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCAC                                                                        >  SRR4302533.4483842/1‑94 (MQ=60)
                        TCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTT                                                                      >  SRR4302533.709987/1‑94 (MQ=60)
                          AGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTG                                                                    <  SRR4302533.5939084/94‑1 (MQ=60)
                          AGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTG                                                                    >  SRR4302533.1879023/1‑94 (MQ=60)
                          AGAGGACGGACGGAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTG                                                                    <  SRR4302533.382341/94‑1 (MQ=60)
                            AGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGG                                                                  <  SRR4302533.2518469/94‑1 (MQ=60)
                             GGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGC                                                                 <  SRR4302533.3192699/94‑1 (MQ=60)
                               ACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAG                                                               >  SRR4302533.3988483/1‑94 (MQ=60)
                               ACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAG                                                               <  SRR4302533.2232612/94‑1 (MQ=60)
                               ACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAG                                                               >  SRR4302533.4766993/1‑94 (MQ=60)
                                CGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGG                                                              >  SRR4302533.612974/1‑94 (MQ=60)
                                 GGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGA                                                             >  SRR4302533.1997505/1‑94 (MQ=60)
                                 GGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGA                                                             >  SRR4302533.6211037/1‑94 (MQ=60)
                                 GGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGA                                                             >  SRR4302533.1969816/1‑94 (MQ=60)
                                  GACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGAC                                                            <  SRR4302533.3442574/94‑1 (MQ=60)
                                  GACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGAC                                                            <  SRR4302533.295920/94‑1 (MQ=60)
                                    CGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTC                                                          >  SRR4302533.1764435/1‑94 (MQ=60)
                                     GTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCA                                                         >  SRR4302533.5236683/1‑94 (MQ=60)
                                     GTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCA                                                         >  SRR4302533.1298194/1‑94 (MQ=60)
                                      TAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCAT                                                        <  SRR4302533.3627284/94‑1 (MQ=60)
                                          CACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATT                                                    <  SRR4302533.5708858/94‑1 (MQ=60)
                                          CACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATT                                                    <  SRR4302533.1851346/94‑1 (MQ=60)
                                           ACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTA                                                   <  SRR4302533.2544081/94‑1 (MQ=60)
                                           ACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTA                                                   <  SRR4302533.2246408/94‑1 (MQ=60)
                                            CACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTAC                                                  <  SRR4302533.4625513/94‑1 (MQ=60)
                                             ACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACG                                                 >  SRR4302533.3756739/1‑94 (MQ=60)
                                             ACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACG                                                 >  SRR4302533.3764801/1‑94 (MQ=60)
                                              CGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGC                                                >  SRR4302533.1577931/1‑94 (MQ=60)
                                                CCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCAC                                              >  SRR4302533.102658/1‑94 (MQ=60)
                                                CCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCAC                                              <  SRR4302533.2580579/94‑1 (MQ=60)
                                                CCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCAC                                              >  SRR4302533.5989002/1‑94 (MQ=60)
                                                 CGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACC                                             >  SRR4302533.477231/1‑94 (MQ=60)
                                                  GAATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCG                                            <  SRR4302533.4417896/94‑1 (MQ=60)
                                                   GATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGT                                           <  SRR4302533.215026/94‑1 (MQ=60)
                                                   GATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGT                                           >  SRR4302533.3259485/1‑94 (MQ=60)
                                                    ATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTTGGCAGGgctccttatttagcaccctg                                          >  SRR4302533.5324032/1‑74 (MQ=60)
                                                    ATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTG                                          <  SRR4302533.4789600/94‑1 (MQ=60)
                                                      ACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAA                                        >  SRR4302533.5956232/1‑94 (MQ=60)
                                                       CTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAAC                                       <  SRR4302533.4571651/94‑1 (MQ=60)
                                                       CTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAAC                                       >  SRR4302533.4499265/1‑94 (MQ=60)
                                                        ctgaaccgctcttccgatctgctgtaTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACA                                      <  SRR4302533.3926282/68‑1 (MQ=60)
                                                        TGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACA                                      <  SRR4302533.5398137/94‑1 (MQ=60)
                                                        TGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAACCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACA                                      <  SRR4302533.6190540/94‑1 (MQ=60)
                                                         GTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAG                                     <  SRR4302533.1293316/94‑1 (MQ=60)
                                                         GTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAG                                     <  SRR4302533.403310/94‑1 (MQ=60)
                                                         GTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAG                                     <  SRR4302533.403302/94‑1 (MQ=60)
                                                         GTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAG                                     >  SRR4302533.4751223/1‑94 (MQ=60)
                                                         GTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAG                                     >  SRR4302533.2221191/1‑94 (MQ=60)
                                                         GTGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAG                                     >  SRR4302533.5178528/1‑94 (MQ=60)
                                                          TGAGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGT                                    <  SRR4302533.3956693/94‑1 (MQ=60)
                                                            AGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGC                                  <  SRR4302533.1210808/94‑1 (MQ=60)
                                                            AGCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGC                                  >  SRR4302533.1671565/1‑94 (MQ=60)
                                                             GCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCT                                 <  SRR4302533.4021618/94‑1 (MQ=60)
                                                             GCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCT                                 <  SRR4302533.193100/94‑1 (MQ=60)
                                                             GCATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCT                                 >  SRR4302533.6008781/1‑94 (MQ=60)
                                                              CATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTG                                >  SRR4302533.2838964/1‑94 (MQ=60)
                                                               ATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGA                               >  SRR4302533.1252687/1‑94 (MQ=60)
                                                               ATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGA                               >  SRR4302533.2169567/1‑94 (MQ=60)
                                                               ATCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGA                               >  SRR4302533.1671263/1‑94 (MQ=60)
                                                                TCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGAT                              <  SRR4302533.5386016/94‑1 (MQ=60)
                                                                TCCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGAT                              <  SRR4302533.1347328/94‑1 (MQ=60)
                                                                 CCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATT                             <  SRR4302533.4263062/94‑1 (MQ=60)
                                                                 CCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATT                             <  SRR4302533.4454620/94‑1 (MQ=60)
                                                                 CCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATT                             >  SRR4302533.5597962/1‑94 (MQ=60)
                                                                 CCAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATT                             <  SRR4302533.3996821/94‑1 (MQ=60)
                                                                  CAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTT                            <  SRR4302533.1620642/94‑1 (MQ=60)
                                                                  CAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTT                            >  SRR4302533.5134453/1‑94 (MQ=60)
                                                                  CAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTT                            <  SRR4302533.197626/94‑1 (MQ=60)
                                                                  CAAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTT                            <  SRR4302533.1252394/94‑1 (MQ=60)
                                                                   AAGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTC                           <  SRR4302533.2249015/94‑1 (MQ=60)
                                                                    AGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGCTTTCG                          >  SRR4302533.322829/1‑94 (MQ=60)
                                                                    AGAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCG                          >  SRR4302533.2326947/1‑94 (MQ=60)
                                                                     GAAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGC                         <  SRR4302533.6034819/94‑1 (MQ=60)
                                                                      AAACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCGCCGTGAACAGTGCTGATTTCGCC                        <  SRR4302533.4373869/94‑1 (MQ=60)
                                                                       AACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCA                       >  SRR4302533.1735485/1‑94 (MQ=60)
                                                                       AACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCA                       >  SRR4302533.5496581/1‑94 (MQ=60)
                                                                        ACGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCAT                      <  SRR4302533.266467/94‑1 (MQ=60)
                                                                         CGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATA                     >  SRR4302533.4568882/1‑94 (MQ=60)
                                                                         CGGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATA                     >  SRR4302533.1570254/1‑94 (MQ=60)
                                                                          GGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGgaggggccacatttttttcaccccgagaaaaagcgatgtttcttta                    >  SRR4302533.3592473/1‑48 (MQ=60)
                                                                          GGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAA                    <  SRR4302533.1104602/94‑1 (MQ=60)
                                                                          GGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAA                    <  SRR4302533.1925341/94‑1 (MQ=60)
                                                                          GGACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAA                    <  SRR4302533.179474/94‑1 (MQ=60)
                                                                            GCCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACA                  <  SRR4302533.3579823/94‑1 (MQ=60)
                                                                            ACCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACA                  >  SRR4302533.4584394/1‑94 (MQ=60)
                                                                             CCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAA                 <  SRR4302533.1360193/94‑1 (MQ=60)
                                                                             CCGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACCCACCTTGAACAGTGCTGATTTCGCCATAAAAA                 >  SRR4302533.2336321/1‑94 (MQ=60)
                                                                              CGTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAAC                >  SRR4302533.3825965/1‑94 (MQ=60)
                                                                               GTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACT               <  SRR4302533.2280660/94‑1 (MQ=60)
                                                                               GTGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACT               >  SRR4302533.2589674/1‑94 (MQ=60)
                                                                                TGTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTT              <  SRR4302533.3123749/94‑1 (MQ=60)
                                                                                 GTTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTA             <  SRR4302533.5821150/94‑1 (MQ=60)
                                                                                  TTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTAT            <  SRR4302533.5497802/94‑1 (MQ=60)
                                                                                  TTTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTAT            <  SRR4302533.6020144/94‑1 (MQ=60)
                                                                                   TTCCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATC           >  SRR4302533.3945104/1‑94 (MQ=60)
                                                                                     CCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCGTAACAACTTATGCT         <  SRR4302533.5008299/94‑1 (MQ=60)
                                                                                     CCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCT         <  SRR4302533.2814849/94‑1 (MQ=60)
                                                                                     CCCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCT         >  SRR4302533.1187526/1‑94 (MQ=60)
                                                                                      CCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTG        <  SRR4302533.703566/94‑1 (MQ=60)
                                                                                      CCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTG        >  SRR4302533.1898552/1‑94 (MQ=60)
                                                                                      CCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTG        <  SRR4302533.5168940/94‑1 (MQ=60)
                                                                                      CCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTG        <  SRR4302533.891155/94‑1 (MQ=60)
                                                                                      CCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTG        >  SRR4302533.6146349/1‑94 (MQ=60)
                                                                                      CCGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTG        <  SRR4302533.4488288/94‑1 (MQ=60)
                                                                                       CGGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGA       <  SRR4302533.5042322/94‑1 (MQ=60)
                                                                                        GGAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAA      >  SRR4302533.5423151/1‑94 (MQ=60)
                                                                                         GAT‑CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAAT     <  SRR4302533.3929807/94‑1 (MQ=60)
                                                                                           atCGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAATT    <  SRR4302533.6303578/92‑1 (MQ=60)
                                                                                           atCGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAATT    <  SRR4302533.1285129/92‑1 (MQ=60)
                                                                                            TCGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAATTA   <  SRR4302533.3523217/94‑1 (MQ=60)
                                                                                             CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAATTAA  <  SRR4302533.276741/94‑1 (MQ=60)
                                                                                             CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAATTAA  >  SRR4302533.6204712/1‑94 (MQ=60)
                                                                                             CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAATTAA  >  SRR4302533.1709916/1‑94 (MQ=60)
                                                                                             CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAATTAA  <  SRR4302533.4739783/94‑1 (MQ=60)
                                                                                             CGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAATTAA  >  SRR4302533.888390/1‑94 (MQ=60)
                                                                                             |                                                                                             
CGATATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTGAGCATCCAAGAAACGGACCGTGTTTCCCGGATCCGAATAACCAGTTCAGCCCCGCACTTTGGGCAGGACTCATTATTACGCACCGTGAACAGTGCTGATTTCGCCATAACAACTTATGCTGAATTAA  >  NC_000913/3429434‑3429620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑