Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,475,807 T→C T110A (ACT→GCT)  slyD ← FKBP‑type peptidyl‑prolyl cis‑trans isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,475,8070TC100.0% 839.9 / NA 261T110A (ACT→GCT) slyDFKBP‑type peptidyl‑prolyl cis‑trans isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (136/125);  total (136/125)

Only 100 of 264 total aligned reads displayed.

GAATCGCCACAACTTCAACGTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGTGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATAAATACGTCTTTAG  >  NC_000913/3475717‑3475896
                                                                                          |                                                                                         
gAATCGCCACAACTTCAACGTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGa                                                                                         <  2:3465628/93‑1 (MQ=255)
 aaTCGCCACAACTTCAACGTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGAt                                                                                        >  1:5217711/1‑93 (MQ=255)
      ccACAACTTCAACGTTGAATTTCAGGTTCTGAGCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCaa                                                                                   >  2:572281/1‑93 (MQ=255)
      ccACAACTTCAACGTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCaa                                                                                   <  1:5413260/93‑1 (MQ=255)
       cacaACTTCAACGTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAAc                                                                                  >  1:3906160/1‑93 (MQ=255)
       cacaACTTCAACGTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAAc                                                                                  >  2:1286632/1‑93 (MQ=255)
       cacaACTTCAACGTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAAc                                                                                  >  1:2341417/1‑93 (MQ=255)
       cacaACTTCAACGTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAAc                                                                                  <  1:1837024/93‑1 (MQ=255)
        acaACTTCAACGTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAAcc                                                                                 <  2:1890294/93‑1 (MQ=255)
        acaACTTCAACGTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAAcc                                                                                 >  2:1429109/1‑93 (MQ=255)
         caACTTCAACGTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCg                                                                                <  2:205257/93‑1 (MQ=255)
          aaCTTCAACGTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCgg                                                                               <  1:3332929/93‑1 (MQ=255)
             ttCAACGTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTAc                                                                            >  2:4983360/1‑93 (MQ=255)
              tCAACGTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTAcc                                                                           >  1:6152665/1‑93 (MQ=255)
                  cGTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGAc                                                                       >  1:4465529/1‑93 (MQ=255)
                   gTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGAcc                                                                      >  1:632452/1‑93 (MQ=255)
                   gTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGAcc                                                                      >  2:2598634/1‑93 (MQ=255)
                    ttGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACACCGTGATGTTCTTCAACCGCAGCGATTTCACCCGGTACCGGAccc                                                                     >  1:1646618/1‑93 (MQ=255)
                     tGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCt                                                                    <  2:1628008/93‑1 (MQ=255)
                     tGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCt                                                                    <  2:4713773/93‑1 (MQ=255)
                     tGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCt                                                                    <  1:1021930/93‑1 (MQ=255)
                     tGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCt                                                                    <  1:1595904/93‑1 (MQ=255)
                       aaTTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTgg                                                                  <  2:3283562/93‑1 (MQ=255)
                       aaTTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTgg                                                                  <  2:3163371/93‑1 (MQ=255)
                        aTTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGt                                                                 <  1:1273750/93‑1 (MQ=255)
                        aTTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGt                                                                 <  2:5837234/93‑1 (MQ=255)
                         tttCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTc                                                                <  1:5665421/93‑1 (MQ=255)
                          ttCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCg                                                               >  1:5803330/1‑93 (MQ=255)
                             aGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGtt                                                            <  1:5123373/93‑1 (MQ=255)
                             aGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGtt                                                            >  2:5274156/1‑93 (MQ=255)
                             aGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGtt                                                            <  2:4513687/93‑1 (MQ=255)
                               gTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTc                                                          <  2:619664/93‑1 (MQ=255)
                                 tCTGACCGGCCAGCATGTGGTTACCATCAATCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAg                                                        >  1:2310749/1‑93 (MQ=255)
                                 tCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGCCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCac                                                        >  1:3606513/1‑92 (MQ=255)
                                   tGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGcc                                                      <  2:3428444/93‑1 (MQ=255)
                                   tGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGcc                                                      >  2:2784288/1‑93 (MQ=255)
                                    gACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCa                                                     <  2:2871967/93‑1 (MQ=255)
                                     aCCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAg                                                    <  2:4779639/93‑1 (MQ=255)
                                     aCCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAg                                                    >  2:2366659/1‑93 (MQ=255)
                                      ccGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCACCCGCAGCGATTTCAACCGGTGCCGGACCCTGGTCGGTTTCAGCCAgg                                                   <  1:3978880/93‑1 (MQ=255)
                                       cGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGa                                                  <  2:3745995/93‑1 (MQ=255)
                                         gCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGaaa                                                <  2:380627/93‑1 (MQ=255)
                                         gCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGaaa                                                <  2:4090594/93‑1 (MQ=255)
                                         gCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGaaa                                                >  2:638064/1‑93 (MQ=255)
                                          ccAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAAc                                               <  1:4532288/93‑1 (MQ=255)
                                           cAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACg                                              >  2:4841397/1‑93 (MQ=255)
                                             gCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCa                                            <  1:4205555/93‑1 (MQ=255)
                                             gCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCa                                            <  1:735442/93‑1 (MQ=255)
                                             gCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCa                                            >  1:3029427/1‑93 (MQ=255)
                                                 gtgGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCAtacc                                        <  1:623263/93‑1 (MQ=255)
                                                  tgGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCAtacct                                       >  1:4386067/1‑93 (MQ=255)
                                                   ggTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCAtaccta                                      >  1:2488975/1‑93 (MQ=255)
                                                   ggTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCAtaccta                                      >  1:4850263/1‑93 (MQ=255)
                                                   ggTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCAtaccta                                      >  1:4795541/1‑93 (MQ=255)
                                                   ggTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCAtaccta                                      <  1:1180746/93‑1 (MQ=255)
                                                    gTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCAtacctac                                     <  2:3769538/93‑1 (MQ=255)
                                                    gTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCAtacctac                                     <  1:4265163/93‑1 (MQ=255)
                                                     ttACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCAtacctacc                                    <  2:2824097/93‑1 (MQ=255)
                                                         cATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCa                                <  1:2690735/93‑1 (MQ=255)
                                                         cATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCa                                <  2:1811396/93‑1 (MQ=255)
                                                         cATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCa                                >  1:3471162/1‑93 (MQ=255)
                                                          aTCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAg                               >  1:4322128/1‑93 (MQ=255)
                                                           tCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGt                              >  1:308143/1‑93 (MQ=255)
                                                           tCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGt                              >  1:6036815/1‑93 (MQ=255)
                                                           tCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGt                              >  1:5203900/1‑93 (MQ=255)
                                                           tCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGt                              >  1:5621677/1‑93 (MQ=255)
                                                             aaCCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTtc                            <  1:2824809/93‑1 (MQ=255)
                                                               ccACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTtcat                          <  2:510390/93‑1 (MQ=255)
                                                                cACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTtcatc                         <  2:3084110/93‑1 (MQ=255)
                                                                cACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTtcatc                         <  2:5590733/93‑1 (MQ=255)
                                                                 acgacgTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTtcatca                        <  2:6086715/93‑1 (MQ=255)
                                                                  cgacgTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCaa                       <  2:4072425/93‑1 (MQ=255)
                                                                  cgacgTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCaa                       <  1:1097509/93‑1 (MQ=255)
                                                                   tacgTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAAc                      <  1:3871972/92‑1 (MQ=255)
                                                                   gacgTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAAc                      <  2:5411162/93‑1 (MQ=255)
                                                                   gacgTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAAc                      >  2:4341103/1‑93 (MQ=255)
                                                                    acgTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACg                     >  2:2731093/1‑93 (MQ=255)
                                                                    acgTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACg                     >  2:4908692/1‑93 (MQ=255)
                                                                      gTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGcc                   >  2:1200707/1‑93 (MQ=255)
                                                                       tGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGccc                  <  2:2568895/93‑1 (MQ=255)
                                                                       ggATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGccc                  <  1:194743/92‑1 (MQ=255)
                                                                        gATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCa                 >  1:3793822/1‑93 (MQ=255)
                                                                        gATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCa                 <  2:4694122/93‑1 (MQ=255)
                                                                         aTCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCAt                <  2:1662767/93‑1 (MQ=255)
                                                                          tCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATa               <  1:1544723/93‑1 (MQ=255)
                                                                           cGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATaa              >  1:1974622/1‑93 (MQ=255)
                                                                            gTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATaaa             >  1:973819/1‑93 (MQ=255)
                                                                              cTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATAAATa           <  1:83029/93‑1 (MQ=255)
                                                                              cTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATAAATa           <  2:5645223/93‑1 (MQ=255)
                                                                               ttCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATAAATAc          <  2:2199191/93‑1 (MQ=255)
                                                                                 cAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATAAATACGt        >  1:4168921/1‑93 (MQ=255)
                                                                                 cAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATAAATACGt        <  1:2052890/93‑1 (MQ=255)
                                                                                   aCCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATAAATACGTCt      >  2:45673/1‑93 (MQ=255)
                                                                                   aCCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATAAATACGTCt      >  1:5307490/1‑93 (MQ=255)
                                                                                   aCCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGACCATAAATACGTCt      >  1:6285234/1‑93 (MQ=255)
                                                                                    ccGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATAAATACGTCtt     <  2:2281568/93‑1 (MQ=255)
                                                                                    ccGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATAAATACGTCtt     <  2:1123425/93‑1 (MQ=255)
                                                                                     cGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATAAATACGTCttt    <  2:5205839/93‑1 (MQ=255)
                                                                                     cGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATAAATACGTCttt    <  2:822956/93‑1 (MQ=255)
                                                                                      gCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATAAATACGTCTTTa   >  1:4666210/1‑93 (MQ=255)
                                                                                          |                                                                                         
GAATCGCCACAACTTCAACGTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGTGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATAAATACGTCTTTAG  >  NC_000913/3475717‑3475896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCGAATCGCCACAACTTCAACGTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGTGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATAAATACGTCTTTAGGAA  >  NC_000913/3475714‑3475899
                                                                                             |                                                                                            
CGCGAATCGCCACAACTTCAACGTTGGATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGC                                                                                              >  SRR4302533.550395/1‑94 (MQ=60)
CGCGAATCGCCACAACTTCAACGTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGC                                                                                              <  SRR4302533.4373768/94‑1 (MQ=60)
CGCGAATCGCCACAACTTCAACGTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGC                                                                                              <  SRR4302533.5613724/94‑1 (MQ=60)
 GCGAATCGCCACAACTTCAACGTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCG                                                                                             <  SRR4302533.1633505/94‑1 (MQ=60)
 GCGAATCGCCACAACTTCAACGTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCG                                                                                             >  SRR4302533.5662799/1‑94 (MQ=60)
 GCGAATCGCCACAACTTCAACGTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCG                                                                                             <  SRR4302533.5681506/94‑1 (MQ=60)
  CGAATCGCCACAACTTCAACGTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGA                                                                                            >  SRR4302533.1987463/1‑94 (MQ=60)
  CGAATCGCCACAACTTCAACGTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGA                                                                                            >  SRR4302533.3907978/1‑94 (MQ=60)
   GAATCGCCACAACTTCAACGTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGAT                                                                                           <  SRR4302533.1100134/94‑1 (MQ=60)
   GAATCGCCACAACTTCAACGTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGAT                                                                                           >  SRR4302533.5249121/1‑94 (MQ=60)
    AATCGCCACAACTTCAACGTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATT                                                                                          >  SRR4302533.6112715/1‑94 (MQ=60)
      TCGCCACAACTTCAACGTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTC                                                                                        <  SRR4302533.6269620/94‑1 (MQ=60)
         CCACAACTTCAACGTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAAC                                                                                     <  SRR4302533.5445811/94‑1 (MQ=60)
         CCACAACTTCAACGTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAAC                                                                                     >  SRR4302533.3930022/1‑94 (MQ=60)
         CCACAACTTCAACGTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAAC                                                                                     >  SRR4302533.5237206/1‑94 (MQ=60)
         CCACAACTTCAACGTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAAC                                                                                     <  SRR4302533.5894709/94‑1 (MQ=60)
         CCACAACTTCAACGTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAAC                                                                                     >  SRR4302533.2355911/1‑94 (MQ=60)
          CACAACTTCAACGTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACC                                                                                    >  SRR4302533.5973492/1‑94 (MQ=60)
          CACAACTTCAACGTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACC                                                                                    <  SRR4302533.1848227/94‑1 (MQ=60)
            CAACTTCAACGTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGG                                                                                  >  SRR4302533.2350747/1‑94 (MQ=60)
             AACTTCAACGTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGT                                                                                 <  SRR4302533.3353523/94‑1 (MQ=60)
             AACTTCAACGTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGT                                                                                 <  SRR4302533.1961075/94‑1 (MQ=60)
              ACTTCAACGTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTA                                                                                <  SRR4302533.5403626/94‑1 (MQ=60)
                TTCAACGTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACC                                                                              >  SRR4302533.5204113/1‑94 (MQ=60)
                TTCAACGTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACC                                                                              >  SRR4302533.6189692/1‑94 (MQ=60)
                 TCAACGTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGAGGTGATCGTCTGCAACCGCAGCGATTTCAACCGGTACCG                                                                             <  SRR4302533.276649/94‑1 (MQ=60)
                  CAACGTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGG                                                                            >  SRR4302533.5695380/1‑94 (MQ=60)
                   AACGTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGA                                                                           >  SRR4302533.2863593/1‑94 (MQ=60)
                    ACGTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGAC                                                                          >  SRR4302533.4492538/1‑94 (MQ=60)
                    ACGTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGAC                                                                          <  SRR4302533.1158723/94‑1 (MQ=60)
                     CGTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACC                                                                         >  SRR4302533.5719292/1‑94 (MQ=60)
                     CGTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACC                                                                         >  SRR4302533.636174/1‑94 (MQ=60)
                     CGTTGAATTCCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCAGGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACC                                                                         <  SRR4302533.2852034/94‑1 (MQ=60)
                      GTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCT                                                                        >  SRR4302533.6342571/1‑94 (MQ=60)
                      GTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACACCGTGATGTTCTTCAACCGCAGCGATTTCACCCGGTACCGGACCC                                                                        >  SRR4302533.1656590/1‑94 (MQ=60)
                        TGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTG                                                                      <  SRR4302533.1605562/94‑1 (MQ=60)
                        TGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTG                                                                      <  SRR4302533.1027934/94‑1 (MQ=60)
                        TGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTG                                                                      >  SRR4302533.5265144/1‑94 (MQ=60)
                         GAATTGCAGGTTCTGACCGGCCAGCATGGGCTTACCATCAGCCACGACGTTATGGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGG                                                                     <  SRR4302533.3406352/94‑1 (MQ=60)
                          AATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGT                                                                    >  SRR4302533.4581342/1‑94 (MQ=60)
                           ATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTC                                                                   <  SRR4302533.1281374/94‑1 (MQ=60)
                            TTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCG                                                                  <  SRR4302533.5699649/94‑1 (MQ=60)
                            TTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCG                                                                  >  SRR4302533.5838383/1‑94 (MQ=60)
                                AGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTT                                                              <  SRR4302533.5154326/94‑1 (MQ=60)
                                  GTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCA                                                            >  SRR4302533.1913869/1‑94 (MQ=60)
                                   TTCTGACCGGCCAGCATGTGGTTACCATCAATCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAG                                                           >  SRR4302533.2325067/1‑94 (MQ=60)
                                   TTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAG                                                           >  SRR4302533.780936/1‑94 (MQ=60)
                                   TTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAG                                                           <  SRR4302533.970471/94‑1 (MQ=60)
                                   TTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGCCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAC                                                           >  SRR4302533.3628715/1‑94 (MQ=60)
                                    TCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGC                                                          >  SRR4302533.3490742/1‑94 (MQ=60)
                                     CTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGCTTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCC                                                         >  SRR4302533.904689/1‑94 (MQ=60)
                                     CTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCC                                                         <  SRR4302533.1769833/94‑1 (MQ=60)
                                       GACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAG                                                       >  SRR4302533.865573/1‑94 (MQ=60)
                                       GACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAG                                                       >  SRR4302533.4004955/1‑94 (MQ=60)
                                         CCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCACCCGCAGCGATTTCAACCGGTGCCGGACCCTGGTCGGTTTCAGCCAGGA                                                     <  SRR4302533.4003104/94‑1 (MQ=60)
                                         CCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGA                                                     >  SRR4302533.4609622/1‑94 (MQ=60)
                                         CCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGA                                                     <  SRR4302533.1638999/94‑1 (MQ=60)
                                          CGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAA                                                    <  SRR4302533.561008/94‑1 (MQ=60)
                                          CGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAA                                                    <  SRR4302533.4204569/94‑1 (MQ=60)
                                           GGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAA                                                   >  SRR4302533.4315729/1‑94 (MQ=60)
                                            GCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAAC                                                  <  SRR4302533.2514118/94‑1 (MQ=60)
                                            GCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAAC                                                  >  SRR4302533.3260311/1‑94 (MQ=60)
                                             CCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACG                                                 <  SRR4302533.4559729/94‑1 (MQ=60)
                                             CCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACG                                                 <  SRR4302533.2070974/94‑1 (MQ=60)
                                              CAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGC                                                >  SRR4302533.2789789/1‑94 (MQ=60)
                                               AGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCA                                               <  SRR4302533.1866367/94‑1 (MQ=60)
                                               AGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCA                                               >  SRR4302533.1592192/1‑94 (MQ=60)
                                               AGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCA                                               >  SRR4302533.3047965/1‑94 (MQ=60)
                                                GCATTTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCAT                                              <  SRR4302533.4453763/94‑1 (MQ=60)
                                                GCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCAT                                              >  SRR4302533.1576781/1‑94 (MQ=60)
                                                GCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCAT                                              <  SRR4302533.739768/94‑1 (MQ=60)
                                                GCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCAT                                              <  SRR4302533.4230996/94‑1 (MQ=60)
                                                  ATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATAC                                            >  SRR4302533.611681/1‑94 (MQ=60)
                                                    GTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCT                                          <  SRR4302533.626923/94‑1 (MQ=60)
                                                    GTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCT                                          <  SRR4302533.1352769/94‑1 (MQ=60)
                                                    GTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCT                                          >  SRR4302533.4412550/1‑94 (MQ=60)
                                                     TGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTA                                         >  SRR4302533.4824591/1‑94 (MQ=60)
                                                     TGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTA                                         >  SRR4302533.2504303/1‑94 (MQ=60)
                                                     TGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTA                                         >  SRR4302533.4879656/1‑94 (MQ=60)
                                                      GGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGCAACGCATACCTAC                                        >  SRR4302533.395813/1‑94 (MQ=60)
                                                      GGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTAC                                        >  SRR4302533.5038983/1‑94 (MQ=60)
                                                      GGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTAC                                        <  SRR4302533.1187839/94‑1 (MQ=60)
                                                       GTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACC                                       <  SRR4302533.4290848/94‑1 (MQ=60)
                                                       GTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACC                                       >  SRR4302533.3881031/1‑94 (MQ=60)
                                                        TTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCT                                      <  SRR4302533.4238500/94‑1 (MQ=60)
                                                        TTACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCT                                      <  SRR4302533.3888194/94‑1 (MQ=60)
                                                         TACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTG                                     <  SRR4302533.6287719/94‑1 (MQ=60)
                                                          ACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGC                                    >  SRR4302533.444586/1‑94 (MQ=60)
                                                          ACCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCAGTTTCAGCCAGGAACCGCATACCTACCTGC                                    >  SRR4302533.4154397/1‑94 (MQ=60)
                                                           CCATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCA                                   >  SRR4302533.3492588/1‑94 (MQ=60)
                                                            CATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAG                                  >  SRR4302533.4348158/1‑94 (MQ=60)
                                                            CATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAG                                  <  SRR4302533.2707231/94‑1 (MQ=60)
                                                             ATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCCGT                                 >  SRR4302533.702688/1‑94 (MQ=60)
                                                             ATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGT                                 >  SRR4302533.5655659/1‑94 (MQ=60)
                                                             ATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGT                                 >  SRR4302533.6073397/1‑94 (MQ=60)
                                                             ATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGT                                 >  SRR4302533.310029/1‑94 (MQ=60)
                                                             ATCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGT                                 >  SRR4302533.5235258/1‑94 (MQ=60)
                                                              TCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTT                                <  SRR4302533.5656005/94‑1 (MQ=60)
                                                              TCAACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTT                                <  SRR4302533.3055423/94‑1 (MQ=60)
                                                                AACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCA                              >  SRR4302533.4240948/1‑94 (MQ=60)
                                                                AACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCA                              >  SRR4302533.630960/1‑94 (MQ=60)
                                                                AACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCA                              >  SRR4302533.808080/1‑94 (MQ=60)
                                                                AACCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCA                              <  SRR4302533.2842193/94‑1 (MQ=60)
                                                                  CCACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATC                            >  SRR4302533.4845590/1‑94 (MQ=60)
                                                                   CACGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCA                           >  SRR4302533.4379702/1‑94 (MQ=60)
                                                                     CGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAAC                         <  SRR4302533.1104009/94‑1 (MQ=60)
                                                                     CGACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAAC                         >  SRR4302533.3243596/1‑94 (MQ=60)
                                                                     CGACGTGATCGTCTTCAACCGCAGCGATTTCAACCCGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAAC                         <  SRR4302533.2366634/94‑1 (MQ=60)
                                                                      TACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACG                        <  SRR4302533.3895605/94‑1 (MQ=60)
                                                                      GACGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACG                        <  SRR4302533.353783/94‑1 (MQ=60)
                                                                        CGTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCC                      <  SRR4302533.2301459/94‑1 (MQ=60)
                                                                         GTGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCC                     >  SRR4302533.1824036/1‑94 (MQ=60)
                                                                          TGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCA                    >  SRR4302533.3817041/1‑94 (MQ=60)
                                                                          TGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCA                    <  SRR4302533.1245213/94‑1 (MQ=60)
                                                                          GGATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCA                    <  SRR4302533.195970/94‑1 (MQ=60)
                                                                            ATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATA                  <  SRR4302533.474895/94‑1 (MQ=60)
                                                                            ATCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATA                  >  SRR4302533.3732304/1‑94 (MQ=60)
                                                                             TCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATAA                 >  SRR4302533.1986715/1‑94 (MQ=60)
                                                                             TCGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATAA                 <  SRR4302533.1554029/94‑1 (MQ=60)
                                                                              CGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATAAA                >  SRR4302533.979536/1‑94 (MQ=60)
                                                                              CGTCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATAAA                >  SRR4302533.4386780/1‑94 (MQ=60)
                                                                                TCTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATAAATA              >  SRR4302533.5267720/1‑94 (MQ=60)
                                                                                 CTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATAAATAC             <  SRR4302533.83556/94‑1 (MQ=60)
                                                                                 CTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATAAATAC             >  SRR4302533.6340638/1‑94 (MQ=60)
                                                                                 CTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATAAATAC             >  SRR4302533.2025503/1‑94 (MQ=60)
                                                                                 CTTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATAAATAC             <  SRR4302533.482153/94‑1 (MQ=60)
                                                                                  TTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATAAATACG            <  SRR4302533.3755113/94‑1 (MQ=60)
                                                                                  TTCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATAAATACG            <  SRR4302533.1104646/94‑1 (MQ=60)
                                                                                   TCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATAAATACGT           >  SRR4302533.4194186/1‑94 (MQ=60)
                                                                                   TCAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATAAATACGT           <  SRR4302533.5520774/94‑1 (MQ=60)
                                                                                    CAACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATAAATACGTC          <  SRR4302533.2065558/94‑1 (MQ=60)
                                                                                     AACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATAAATACGTCT         >  SRR4302533.5339299/1‑94 (MQ=60)
                                                                                     AACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATAAATACGTCT         <  SRR4302533.2503070/94‑1 (MQ=60)
                                                                                     AACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATAAATACGTCT         <  SRR4302533.3966381/94‑1 (MQ=60)
                                                                                     AACCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGACCATAAATACGTCT         >  SRR4302533.6322737/1‑94 (MQ=60)
                                                                                       CCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATAAATACGTCTTT       <  SRR4302533.1459708/94‑1 (MQ=60)
                                                                                       CCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATAAATACGTCTTT       <  SRR4302533.5161571/94‑1 (MQ=60)
                                                                                       CCGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATAAATACGTCTTT       <  SRR4302533.4556656/94‑1 (MQ=60)
                                                                                        CGCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATAAATACGTCTTTA      >  SRR4302533.4694482/1‑94 (MQ=60)
                                                                                         GCAGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATAAATACGTCTTTAG     >  SRR4302533.4943281/1‑94 (MQ=60)
                                                                                           AGCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATAAATACGTCTTTAGGA   <  SRR4302533.758149/94‑1 (MQ=60)
                                                                                            GCGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATAAATACGTCTTTAGGAA  <  SRR4302533.830468/94‑1 (MQ=60)
                                                                                             |                                                                                            
CGCGAATCGCCACAACTTCAACGTTGAATTTCAGGTTCTGACCGGCCAGCATGTGGTTACCATCAACCACGACGTGATCGTCTTCAACCGCAGTGATTTCAACCGGTACCGGACCCTGGTCGGTTTCAGCCAGGAAACGCATACCTACCTGCAGTTCATCAACGCCCATAAATACGTCTTTAGGAA  >  NC_000913/3475714‑3475899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑