Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,683,433 T→C Y1107C (TAC→TGC)  b3530 ← putative oxidoreductase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,683,4330TC100.0% 626.4 / NA 195Y1107C (TAC→TGC) b3530putative oxidoreductase subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (91/104);  total (91/104)

Only 100 of 211 total aligned reads displayed.

TAAGGTATCAGCGGCTGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGTAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCG  >  NC_000913/3683343‑3683522
                                                                                          |                                                                                         
 aaGGTATCAGCGGCTGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAAt                                                                                        <  2:2418006/93‑1 (MQ=255)
 aaGGTATCAGCGGCTGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAAt                                                                                        >  2:1952794/1‑93 (MQ=255)
  aGGTATCAGCGGCTGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATc                                                                                       >  1:3876914/1‑93 (MQ=255)
  aGGTATCAGCGGCTGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATc                                                                                       <  1:3028980/93‑1 (MQ=255)
     tATCAGCGGCTGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCttt                                                                                    <  2:2393955/93‑1 (MQ=255)
     tATCAGCGGCTGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCttt                                                                                    >  2:2093657/1‑93 (MQ=255)
      aTCAGCGGCTGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATCACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATGTTTc                                                                                   >  2:308424/1‑93 (MQ=255)
      aTCAGCGGCTGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTc                                                                                   <  1:5093973/93‑1 (MQ=255)
      aTCAGCGGCTGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTc                                                                                   <  2:24444/93‑1 (MQ=255)
      aTCAGCGGCTGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTc                                                                                   >  1:2315939/1‑93 (MQ=255)
       tCAGCGGCTGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCg                                                                                  >  2:1558817/1‑93 (MQ=255)
       tCAGCGGCTGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCg                                                                                  <  1:5077382/93‑1 (MQ=255)
          gCGGCTGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCct                                                                               >  2:4335453/1‑93 (MQ=255)
          gCGGCTGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCct                                                                               <  1:464213/93‑1 (MQ=255)
            ggCTGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGGGCAATCTTTCGCctgc                                                                             >  2:436438/1‑93 (MQ=255)
            ggCTGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCctgc                                                                             <  1:5709582/93‑1 (MQ=255)
            ggCTGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCctgc                                                                             <  2:5112332/93‑1 (MQ=255)
            ggCTGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCctgc                                                                             <  1:1317354/93‑1 (MQ=255)
             gCTGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCctgct                                                                            >  1:5389525/1‑93 (MQ=255)
               tGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTgg                                                                          <  1:4115356/93‑1 (MQ=255)
               tGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGGGCGCAATCTTTCGCCTGCTgg                                                                          >  2:2527090/1‑93 (MQ=255)
                 cggcggTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGat                                                                        <  1:5377038/93‑1 (MQ=255)
                 cggcggTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGat                                                                        <  2:2202097/93‑1 (MQ=255)
                  ggcggTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGata                                                                       >  1:2113939/1‑93 (MQ=255)
                    cggTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATc                                                                     <  1:4768196/93‑1 (MQ=255)
                    cggTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATc                                                                     >  1:1427381/1‑93 (MQ=255)
                     ggTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATCACGTACGTAGAGCAGGAAATGGCTGGGTGGGCAATCTTTCTCCTGCTGGTTATCa                                                                    >  1:3365126/1‑93 (MQ=255)
                     ggTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCa                                                                    >  1:856505/1‑93 (MQ=255)
                      gTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCaa                                                                   <  2:4963791/93‑1 (MQ=255)
                      gTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCaa                                                                   >  2:2297431/1‑93 (MQ=255)
                      gTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCaa                                                                   <  2:368681/93‑1 (MQ=255)
                      gTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCaa                                                                   <  1:5884797/93‑1 (MQ=255)
                      gTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCaa                                                                   >  1:2384568/1‑93 (MQ=255)
                       tAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAAt                                                                  <  2:5839454/93‑1 (MQ=255)
                        aaaTCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAAtt                                                                 <  2:4855951/93‑1 (MQ=255)
                            ccATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCg                                                             >  1:3589372/1‑93 (MQ=255)
                            ccATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCg                                                             >  1:2429562/1‑93 (MQ=255)
                            ccATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTAGCCTGCTGGATATCAATTGCCg                                                             >  1:737426/1‑93 (MQ=255)
                               tGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGc                                                          >  1:2846259/1‑93 (MQ=255)
                               tGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGc                                                          >  2:6257484/1‑93 (MQ=255)
                                gTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGcc                                                         <  1:2249680/93‑1 (MQ=255)
                                 tCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCca                                                        >  1:2998828/1‑93 (MQ=255)
                                    cccTGCCATCCGGCGGCAGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCcaaca                                                     >  1:2081723/1‑93 (MQ=255)
                                      cTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACaaa                                                   <  1:317210/93‑1 (MQ=255)
                                        gCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAAcc                                                 >  1:2720180/1‑93 (MQ=255)
                                        gCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAAcc                                                 <  1:5496009/93‑1 (MQ=255)
                                        gCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAAcc                                                 >  2:1027755/1‑93 (MQ=255)
                                         ccATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCa                                                <  2:5942683/93‑1 (MQ=255)
                                         ccATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCa                                                <  2:4722446/93‑1 (MQ=255)
                                          cATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGGCAACAAACCAg                                               >  2:2329206/1‑93 (MQ=255)
                                          cATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAg                                               <  1:1096650/93‑1 (MQ=255)
                                            tCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGtt                                             <  2:5633242/93‑1 (MQ=255)
                                            tCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGtt                                             <  2:2044801/93‑1 (MQ=255)
                                               ggcggcggAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGa                                          >  2:4816863/1‑93 (MQ=255)
                                                gcggcggAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGaa                                         <  2:5171814/93‑1 (MQ=255)
                                                 gggcggAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAg                                        <  2:4480747/92‑1 (MQ=255)
                                                 cggcggAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAg                                        >  2:5784983/1‑93 (MQ=255)
                                                  ggcggAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGt                                       <  1:711716/93‑1 (MQ=255)
                                                   gcggAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTa                                      >  2:5324949/1‑93 (MQ=255)
                                                   gcggAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTa                                      >  2:6097655/1‑93 (MQ=255)
                                                   gcggAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTa                                      >  2:3393552/1‑93 (MQ=255)
                                                    cggAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTaa                                     <  1:2013119/93‑1 (MQ=255)
                                                     ggAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTaaa                                    >  2:1225340/1‑92 (MQ=255)
                                                     ggAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAac                                    <  2:1860850/93‑1 (MQ=255)
                                                     ggAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAac                                    >  1:5461292/1‑93 (MQ=255)
                                                      gAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAaca                                   >  2:647802/1‑93 (MQ=255)
                                                       aaTAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAacac                                  >  1:5813117/1‑93 (MQ=255)
                                                       aaTAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAacac                                  >  1:334224/1‑93 (MQ=255)
                                                        aTAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACAcg                                 >  2:1744497/1‑93 (MQ=255)
                                                         tAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACAcgt                                >  1:4316799/1‑93 (MQ=255)
                                                          aaCGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACAcgtc                               >  2:5126281/1‑93 (MQ=255)
                                                             gtacgtaGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCTAACAAACCAGTTGGAAGTAACACGTCGtt                            >  1:1971316/1‑93 (MQ=255)
                                                             gtacgtaGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGtt                            <  1:5965476/93‑1 (MQ=255)
                                                              tacgtaGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTc                           >  1:1569998/1‑93 (MQ=255)
                                                               acgtaGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCa                          <  1:159646/93‑1 (MQ=255)
                                                                cgtaGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCaa                         >  2:3015462/1‑93 (MQ=255)
                                                                 gtaGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTTCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAg                        <  1:3717765/93‑1 (MQ=255)
                                                                  taGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGt                       >  2:790743/1‑93 (MQ=255)
                                                                  taGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGt                       <  1:4516108/93‑1 (MQ=255)
                                                                   agagCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTa                      <  2:4385848/93‑1 (MQ=255)
                                                                    gagCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTaa                     <  1:1200693/93‑1 (MQ=255)
                                                                    gagCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTaa                     >  2:4214974/1‑93 (MQ=255)
                                                                    gagCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTaa                     >  2:4143172/1‑93 (MQ=255)
                                                                     agCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAAt                    <  1:5997739/93‑1 (MQ=255)
                                                                      gCAGGAAATGGCTGGGGGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCCAGTAATg                   >  1:5599599/1‑93 (MQ=255)
                                                                         ggAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGccc                <  2:4594426/93‑1 (MQ=255)
                                                                         ggAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGccc                <  2:5554905/93‑1 (MQ=255)
                                                                         ggAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGccc                <  2:1049815/93‑1 (MQ=255)
                                                                         ggAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGccc                <  2:2139742/93‑1 (MQ=255)
                                                                           aaaTGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCcgc              <  1:2205750/93‑1 (MQ=255)
                                                                           aaaTGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCcgc              <  1:1914729/93‑1 (MQ=255)
                                                                           aaaTGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCcgc              >  2:3174723/1‑93 (MQ=255)
                                                                               ggCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCgcgctg          <  2:6031769/93‑3 (MQ=255)
                                                                               ggCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCg          >  2:3823158/1‑93 (MQ=255)
                                                                                  tGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCgtgt       >  1:4131143/1‑93 (MQ=255)
                                                                                   gggTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTa      <  2:1002453/93‑1 (MQ=255)
                                                                                   gggTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTa      <  1:3657508/93‑1 (MQ=255)
                                                                                    ggTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAg     >  1:6076557/1‑93 (MQ=255)
                                                                                       gcgcAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCg  <  1:2514298/93‑1 (MQ=255)
                                                                                       gcgcAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCg  <  1:2481800/93‑1 (MQ=255)
                                                                                          |                                                                                         
TAAGGTATCAGCGGCTGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGTAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCG  >  NC_000913/3683343‑3683522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGTAAGGTATCAGCGGCTGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGTAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGC  >  NC_000913/3683340‑3683526
                                                                                             |                                                                                             
GCGTAAGGTATCAGCGGCTGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGC                                                                                               >  SRR4302533.1942527/1‑94 (MQ=60)
 CGTAAGGTATCAGCGGCTGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCA                                                                                              >  SRR4302533.5312904/1‑94 (MQ=60)
 CGTAAGGTATCAGCGGCTGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCA                                                                                              >  SRR4302533.2010708/1‑94 (MQ=60)
 CGTAAGGTATCAGCGGCTGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCA                                                                                              >  SRR4302533.547196/1‑94 (MQ=60)
  GTAAGGTATCAGCGGCTGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAA                                                                                             >  SRR4302533.5445224/1‑94 (MQ=60)
  GTAAGGTATCAGCGGCTGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGGGCGCAA                                                                                             >  SRR4302533.2479334/1‑94 (MQ=60)
   TAAGGTATCAGCGGCTGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAAT                                                                                            <  SRR4302533.2342275/94‑1 (MQ=60)
    AAGGTATCAGCGGCTGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATC                                                                                           >  SRR4302533.3900575/1‑94 (MQ=60)
     AGGTATCAGCGGCTGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCT                                                                                          <  SRR4302533.3047514/94‑1 (MQ=60)
        TATCAGCGGCTGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTC                                                                                       >  SRR4302533.2330280/1‑94 (MQ=60)
        TATCAGCGGCTGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTC                                                                                       <  SRR4302533.2988006/94‑1 (MQ=60)
        TATCAGCGGCTGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTC                                                                                       <  SRR4302533.5118273/94‑1 (MQ=60)
        TATCAGCGGCTGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTC                                                                                       <  SRR4302533.5683493/94‑1 (MQ=60)
        TATCAGCGGCTGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTC                                                                                       <  SRR4302533.2177981/94‑1 (MQ=60)
         ATCAGCGGCTGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCG                                                                                      <  SRR4302533.5124744/94‑1 (MQ=60)
         ATCAGCGGCTGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCG                                                                                      >  SRR4302533.1954769/1‑94 (MQ=60)
          TCAGCGGCTGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGC                                                                                     <  SRR4302533.5108072/94‑1 (MQ=60)
            AGCGGCTGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCT                                                                                   >  SRR4302533.1586770/1‑94 (MQ=60)
             GCGGCTGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTG                                                                                  <  SRR4302533.466982/94‑1 (MQ=60)
               GGCTGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCT                                                                                <  SRR4302533.1325257/94‑1 (MQ=60)
               GGCTGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCT                                                                                >  SRR4302533.5421912/1‑94 (MQ=60)
               GGCTGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCT                                                                                <  SRR4302533.5744070/94‑1 (MQ=60)
                 CTGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGG                                                                              <  SRR4302533.2517329/94‑1 (MQ=60)
                  TGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGA                                                                             <  SRR4302533.4140361/94‑1 (MQ=60)
                    CGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATA                                                                           <  SRR4302533.5409353/94‑1 (MQ=60)
                    CGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATA                                                                           >  SRR4302533.2126974/1‑94 (MQ=60)
                      GCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATC                                                                         >  SRR4302533.1436008/1‑94 (MQ=60)
                       CGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATCACGTACGTAGAGCAGGAAATGGCTGGGTGGGCAATCTTTCTCCTGCTGGTTATCA                                                                        >  SRR4302533.3385913/1‑94 (MQ=60)
                       CGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCA                                                                        <  SRR4302533.4797099/94‑1 (MQ=60)
                       CGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCA                                                                        >  SRR4302533.861520/1‑94 (MQ=60)
                        GGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAA                                                                       >  SRR4302533.3876831/1‑94 (MQ=60)
                        GGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAA                                                                       >  SRR4302533.2399306/1‑94 (MQ=60)
                         GTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAAT                                                                      <  SRR4302533.5920359/94‑1 (MQ=60)
                          TAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATT                                                                     <  SRR4302533.4511825/94‑1 (MQ=60)
                          TAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATT                                                                     <  SRR4302533.2827407/94‑1 (MQ=60)
                           AAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTG                                                                    >  SRR4302533.5613015/1‑94 (MQ=60)
                              TCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCG                                                                 >  SRR4302533.3611470/1‑94 (MQ=60)
                              TCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCG                                                                 >  SRR4302533.2444581/1‑94 (MQ=60)
                              TCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCG                                                                 <  SRR4302533.5313754/94‑1 (MQ=60)
                              TCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCG                                                                 <  SRR4302533.6029648/94‑1 (MQ=60)
                              TCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTAGCCTGCTGGATATCAATTGCCG                                                                 >  SRR4302533.741768/1‑94 (MQ=60)
                                CATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTG                                                               <  SRR4302533.3703955/94‑1 (MQ=60)
                                 ATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGC                                                              >  SRR4302533.2863777/1‑94 (MQ=60)
                                   GTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCA                                                            <  SRR4302533.2263584/94‑1 (MQ=60)
                                   GTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCA                                                            >  SRR4302533.3017167/1‑94 (MQ=60)
                                      ACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACA                                                         >  SRR4302533.2759408/1‑94 (MQ=60)
                                      ACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACA                                                         >  SRR4302533.4339220/1‑94 (MQ=60)
                                      ACCCTGCCATCCGGCGGCAGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACA                                                         >  SRR4302533.2094535/1‑94 (MQ=60)
                                        CCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAA                                                       <  SRR4302533.2124261/94‑1 (MQ=60)
                                         CTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAAC                                                      <  SRR4302533.319151/94‑1 (MQ=60)
                                         CTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAAC                                                      >  SRR4302533.6244639/1‑94 (MQ=60)
                                          TGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACC                                                     >  SRR4302533.2736876/1‑94 (MQ=60)
                                          TGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACC                                                     <  SRR4302533.5914452/94‑1 (MQ=60)
                                           GCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCA                                                    <  SRR4302533.5529157/94‑1 (MQ=60)
                                            CCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAG                                                   >  SRR4302533.2057128/1‑94 (MQ=60)
                                            CCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAG                                                   >  SRR4302533.3868669/1‑94 (MQ=60)
                                             CATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGT                                                  <  SRR4302533.1103145/94‑1 (MQ=60)
                                               TCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTG                                                <  SRR4302533.2652941/94‑1 (MQ=60)
                                                CCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGG                                               <  SRR4302533.3613003/94‑1 (MQ=60)
                                                CCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCATGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGG                                               <  SRR4302533.1311837/94‑1 (MQ=60)
                                                 CGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGA                                              <  SRR4302533.3211416/94‑1 (MQ=60)
                                                 CGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGA                                              <  SRR4302533.1546380/94‑1 (MQ=60)
                                                   GCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAG                                            <  SRR4302533.4149242/94‑1 (MQ=60)
                                                   GCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAG                                            <  SRR4302533.5496134/94‑1 (MQ=60)
                                                     GGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTA                                          >  SRR4302533.3100951/1‑94 (MQ=60)
                                                     GGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTA                                          <  SRR4302533.715919/94‑1 (MQ=60)
                                                     GGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTA                                          >  SRR4302533.2283566/1‑94 (MQ=60)
                                                      GCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAA                                         <  SRR4302533.3926203/94‑1 (MQ=60)
                                                       CGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAAC                                        >  SRR4302533.5494194/1‑94 (MQ=60)
                                                       CGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAAC                                        >  SRR4302533.3521069/1‑94 (MQ=60)
                                                       CGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAAC                                        <  SRR4302533.2025518/94‑1 (MQ=60)
                                                       CGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAAC                                        <  SRR4302533.2238108/94‑1 (MQ=60)
                                                        GGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACA                                       >  SRR4302533.5987421/1‑94 (MQ=60)
                                                        GGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACA                                       <  SRR4302533.2009514/94‑1 (MQ=60)
                                                         GAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACAC                                      <  SRR4302533.5417766/94‑1 (MQ=60)
                                                         GAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACAC                                      >  SRR4302533.336267/1‑94 (MQ=60)
                                                         GAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACAC                                      >  SRR4302533.5848225/1‑94 (MQ=60)
                                                         GAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACAC                                      <  SRR4302533.4832204/94‑1 (MQ=60)
                                                          AATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACG                                     <  SRR4302533.2416013/94‑1 (MQ=60)
                                                           ATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGT                                    >  SRR4302533.4342793/1‑94 (MQ=60)
                                                            TAACGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTC                                   <  SRR4302533.6055818/94‑1 (MQ=60)
                                                               CGTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCTAACAAACCAGTTGGAAGTAACACGTCGTT                                >  SRR4302533.1983378/1‑94 (MQ=60)
                                                                GTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTC                               >  SRR4302533.1579456/1‑94 (MQ=60)
                                                                GTACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTC                               <  SRR4302533.6001575/94‑1 (MQ=60)
                                                                  ACGTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAA                             <  SRR4302533.160666/94‑1 (MQ=60)
                                                                    GTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTTCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGT                           <  SRR4302533.3740584/94‑1 (MQ=60)
                                                                    GTAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACACACCAGTTGGAAGTAACACGTCGTTCAAGT                           >  SRR4302533.1424714/1‑94 (MQ=60)
                                                                     TAGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTA                          <  SRR4302533.4543453/94‑1 (MQ=60)
                                                                      AGAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAA                         <  SRR4302533.2122028/94‑1 (MQ=60)
                                                                       GAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAAT                        <  SRR4302533.1207905/94‑1 (MQ=60)
                                                                       GAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAAT                        <  SRR4302533.3374035/94‑1 (MQ=60)
                                                                       GAGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAAT                        <  SRR4302533.6013454/94‑1 (MQ=60)
                                                                        AGCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATG                       <  SRR4302533.6034064/94‑1 (MQ=60)
                                                                        AGCAGGAAATGGCTGGGGGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCCAGTAATG                       >  SRR4302533.5633431/1‑94 (MQ=60)
                                                                         GCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGC                      <  SRR4302533.4692491/94‑1 (MQ=60)
                                                                         GCAGGAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGGGCCAACAAACCAGTTGGAAGTAACACGTCGTTCATGTAATGC                      >  SRR4302533.4996844/1‑94 (MQ=60)
                                                                             GAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGC                  >  SRR4302533.740422/1‑94 (MQ=60)
                                                                             GAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGC                  <  SRR4302533.11659/94‑1 (MQ=60)
                                                                             GAAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGC                  <  SRR4302533.4634565/94‑1 (MQ=60)
                                                                              AAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCG                 <  SRR4302533.1926415/94‑1 (MQ=60)
                                                                              AAATGGCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCG                 <  SRR4302533.2219353/94‑1 (MQ=60)
                                                                                   GCTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTG            <  SRR4302533.1682059/94‑1 (MQ=60)
                                                                                    CTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGT           >  SRR4302533.5200548/1‑94 (MQ=60)
                                                                                    CTGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGT           >  SRR4302533.4156218/1‑94 (MQ=60)
                                                                                     TGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTA          >  SRR4302533.5748022/1‑94 (MQ=60)
                                                                                     TGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTA          >  SRR4302533.794011/1‑94 (MQ=60)
                                                                                     TGGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTA          <  SRR4302533.3036828/94‑1 (MQ=60)
                                                                                      GGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAG         <  SRR4302533.3679995/94‑1 (MQ=60)
                                                                                      GGGTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAG         >  SRR4302533.6113309/1‑94 (MQ=60)
                                                                                        GTGCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCC       >  SRR4302533.910916/1‑94 (MQ=60)
                                                                                          GCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGA     <  SRR4302533.2529790/94‑1 (MQ=60)
                                                                                          GCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGA     <  SRR4302533.2497105/94‑1 (MQ=60)
                                                                                          GCGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGA     >  SRR4302533.3615377/1‑94 (MQ=60)
                                                                                           CGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAG    >  SRR4302533.5294609/1‑94 (MQ=60)
                                                                                           CGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAA    <  SRR4302533.406567/94‑1 (MQ=60)
                                                                                           CGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAA    <  SRR4302533.438507/94‑1 (MQ=60)
                                                                                           CGCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAA    >  SRR4302533.5827025/1‑94 (MQ=60)
                                                                                            GCAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAG   <  SRR4302533.2868498/94‑1 (MQ=60)
                                                                                             CAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGC  >  SRR4302533.2213819/1‑94 (MQ=60)
                                                                                             CAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGC  <  SRR4302533.889590/94‑1 (MQ=60)
                                                                                             |                                                                                             
GCGTAAGGTATCAGCGGCTGCGGCGGTAAATCCATGTCACCCTGCCATCCGGCGGCGGAATAACGTACGTAGAGCAGGAAATGGCTGGGTGCGTAATCTTTCGCCTGCTGGATATCAATTGCCGTGCCAACAAACCAGTTGGAAGTAACACGTCGTTCAAGTAATGCCCGCGCCGTGTAGCCGAAGC  >  NC_000913/3683340‑3683526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑