Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,735,415	G→A	R97H (CGC→CAC)	malS →	alpha‑amylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,735,415	0	G	A	99.6%	825.9 / NA	242	R97H (CGC→CAC)	malS	alpha‑amylase
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (113/128); minor base C (0/1); total (113/129)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 252 total aligned reads displayed.

ATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGAT  >  NC_000913/3735325‑3735504

aTCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACt                                                                                         <  2:6202984/93‑1 (MQ=255)

  cAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTcc                                                                                       <  1:345336/93‑1 (MQ=255)

  cAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTcc                                                                                       >  2:1343567/1‑93 (MQ=255)

   aGATGCTTTCCCTCCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCg                                                                                      <  1:2763336/93‑1 (MQ=255)

    gATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCgg                                                                                     <  2:3703303/93‑1 (MQ=255)

    gATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCgg                                                                                     >  1:5228813/1‑93 (MQ=255)

    gATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCgg                                                                                     >  1:433364/1‑93 (MQ=255)

      tGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTa                                                                                   <  1:5859437/93‑1 (MQ=255)

       gCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCGAATAGACACCCACTCCGGTAc                                                                                  >  1:1387829/1‑93 (MQ=255)

        cTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACg                                                                                 >  2:3310762/1‑93 (MQ=255)

        cTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACg                                                                                 >  2:2305818/1‑93 (MQ=255)

         tttCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGc                                                                                >  2:4320128/1‑93 (MQ=255)

         tttCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGc                                                                                <  2:542485/93‑1 (MQ=255)

          ttCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGcc                                                                               <  1:5171014/93‑1 (MQ=255)

           tCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCa                                                                              <  1:1406105/93‑1 (MQ=255)

              cTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACa                                                                           <  1:2785749/93‑1 (MQ=255)

               tGCAGCCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACAt                                                                          <  2:2663774/93‑1 (MQ=255)

               tGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACAt                                                                          <  2:5055627/93‑1 (MQ=255)

               tGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACAt                                                                          <  2:1715079/93‑1 (MQ=255)

                gCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACAtt                                                                         >  1:2863488/1‑93 (MQ=255)

                 cAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATtg                                                                        <  1:5695501/93‑1 (MQ=255)

                 cAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATtg                                                                        >  1:3370267/1‑93 (MQ=255)

                  aaCCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATtga                                                                       <  2:4369447/93‑1 (MQ=255)

                   aCCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATtgat                                                                      <  1:903085/93‑1 (MQ=255)

                   aCCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATtgat                                                                      <  2:4994692/93‑1 (MQ=255)

                    ccTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATtgatg                                                                     >  1:3502360/1‑93 (MQ=255)

                     cTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATtgatga                                                                    >  2:1644886/1‑93 (MQ=255)

                       tGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGAtt                                                                  <  1:3857075/93‑1 (MQ=255)

                       tGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGAtt                                                                  <  2:2206115/93‑1 (MQ=255)

                        gTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGAttt                                                                 <  2:4708312/93‑1 (MQ=255)

                         tAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTtc                                                                <  1:2316955/93‑1 (MQ=255)

                          aGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTtcc                                                               <  2:2834311/93‑1 (MQ=255)

                          aGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTtcc                                                               <  2:4243146/93‑1 (MQ=255)

                           gCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTtcca                                                              >  2:5102344/1‑93 (MQ=255)

                             aaCACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTtccatc                                                            >  1:4490301/1‑93 (MQ=255)

                              acacGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACCCCCACTCCGGTACGCCAACATTGATGATTtccatcc                                                           <  1:1738408/93‑1 (MQ=255)

                              acacGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTtccatcc                                                           <  2:52428/93‑1 (MQ=255)

                               cacGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTtccatcca                                                          <  1:2489711/93‑1 (MQ=255)

                                acGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAg                                                         >  1:4800832/1‑93 (MQ=255)

                                 cgccgcCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGa                                                        <  2:2537889/93‑1 (MQ=255)

                                   ccgccTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAAc                                                      >  2:5737160/1‑93 (MQ=255)

                                     gccTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAAcgc                                                    <  1:5622282/93‑1 (MQ=255)

                                     gccTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAAcgc                                                    <  1:200457/93‑1 (MQ=255)

                                      ccTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAAcgcc                                                   <  2:3255017/93‑1 (MQ=255)

                                      ccTCAATGGCGATTGTGCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAAcgcc                                                   <  1:211776/93‑1 (MQ=255)

                                       cTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAAcgccg                                                  >  1:1219078/1‑93 (MQ=255)

                                        tCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAAcgccgc                                                 <  1:4370483/93‑1 (MQ=255)

                                        tCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAAcgccgc                                                 >  2:2113960/1‑93 (MQ=255)

                                             ggCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAAc                                            <  2:5910416/93‑1 (MQ=255)

                                              gCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAAcc                                           >  2:5346840/1‑93 (MQ=255)

                                                gATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCgg                                         >  2:5496167/1‑93 (MQ=255)

                                                 aTTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGt                                        <  2:861220/93‑1 (MQ=255)

                                                  ttGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTa                                       <  1:3078394/93‑1 (MQ=255)

                                                  ttGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTa                                       <  2:4781824/93‑1 (MQ=255)

                                                   tGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTAGGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAg                                      <  1:1885887/93‑1 (MQ=255)

                                                   tGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAg                                      >  1:4586926/1‑93 (MQ=255)

                                                    gTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGc                                     >  1:1855084/1‑93 (MQ=255)

                                                      tCAGGGACGGCGAATATACGCTGTAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCaa                                   <  1:1601011/93‑1 (MQ=255)

                                                      tCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCaa                                   >  1:1156411/1‑93 (MQ=255)

                                                      tCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCaa                                   <  1:5485062/93‑1 (MQ=255)

                                                        aGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGc                                 <  2:1813382/93‑1 (MQ=255)

                                                        aGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGc                                 <  2:2404023/93‑1 (MQ=255)

                                                         gggACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGcc                                >  2:1532445/1‑93 (MQ=255)

                                                            aCGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTgg                             <  1:711257/93‑1 (MQ=255)

                                                            aCGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTgg                             <  1:5485548/93‑1 (MQ=255)

                                                             cGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGt                            <  2:5630020/93‑1 (MQ=255)

                                                               gCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTcc                          <  1:4097919/93‑1 (MQ=255)

                                                               gCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTcc                          <  1:2228160/93‑1 (MQ=255)

                                                                cGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCg                         >  1:6162353/1‑93 (MQ=255)

                                                                cGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCg                         <  1:849339/93‑1 (MQ=255)

                                                                 gAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGt                        <  2:3958558/93‑1 (MQ=255)

                                                                  aaTATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTg                       >  2:513303/1‑93 (MQ=255)

                                                                    tataCGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGaa                     <  1:1103428/93‑1 (MQ=255)

                                                                    tataCGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGaa                     <  1:4734018/93‑1 (MQ=255)

                                                                    tataCGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGaa                     <  2:3062899/93‑1 (MQ=255)

                                                                     ataCGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAAt                    >  1:1029126/1‑93 (MQ=255)

                                                                     ataCGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAAt                    <  1:1457171/93‑1 (MQ=255)

                                                                     ataCGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAAt                    >  2:1293581/1‑93 (MQ=255)

                                                                      taCGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATg                   >  2:4673115/1‑93 (MQ=255)

                                                                       aCGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGc                  <  1:2466901/93‑1 (MQ=255)

                                                                         gCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGccc                >  1:997536/1‑93 (MQ=255)

                                                                         gCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGccc                >  1:5036327/1‑93 (MQ=255)

                                                                         gCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGccc                >  2:393576/1‑93 (MQ=255)

                                                                           tGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGa              >  1:3718679/1‑93 (MQ=255)

                                                                              aaaTAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAAt           >  1:3680191/1‑93 (MQ=255)

                                                                              aaaTAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAAt           <  1:2554629/93‑1 (MQ=255)

                                                                              aaaTAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAAt           <  2:3395624/93‑1 (MQ=255)

                                                                              aaaTAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAAt           >  2:6078644/1‑93 (MQ=255)

                                                                              aaaTAGACACCCACTCCGGTACGCCAACATTGATGATTCCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAAt           <  1:2682676/93‑1 (MQ=255)

                                                                               aaTAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATg          <  2:2178780/93‑1 (MQ=255)

                                                                               aaTAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATg          <  2:4155493/93‑1 (MQ=255)

                                                                               aaTAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATg          <  1:1560488/93‑1 (MQ=255)

                                                                                aTAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATgg         <  2:49038/93‑1 (MQ=255)

                                                                                aTAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATgg         <  2:1114458/93‑1 (MQ=255)

                                                                                aTAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATgg         <  2:366296/93‑1 (MQ=255)

                                                                                 tAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATggg        <  2:6292081/93‑1 (MQ=255)

                                                                                  aGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGgga       <  1:4964171/93‑1 (MQ=255)

                                                                                      acCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGggatgga   >  2:2235667/1‑93 (MQ=255)

                                                                                       cccACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGggatggat  >  2:2915392/1‑93 (MQ=255)

                                                                                       cccACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGggatggat  >  2:3894060/1‑93 (MQ=255)

ATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGAT  >  NC_000913/3735325‑3735504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TCAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTACC  >  NC_000913/3735322‑3735508

TCAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCA                                                                                               <  SRR4302533.5172020/94‑1 (MQ=60)

TCAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCA                                                                                               <  SRR4302533.3745331/94‑1 (MQ=60)

TCAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCA                                                                                               >  SRR4302533.632273/1‑94 (MQ=60)

 CAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCAC                                                                                              <  SRR4302533.1371404/94‑1 (MQ=60)

   ATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTC                                                                                            >  SRR4302533.2297304/1‑94 (MQ=60)

    TCAGATGCTGTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCC                                                                                           <  SRR4302533.3428248/94‑1 (MQ=60)

     CAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCG                                                                                          <  SRR4302533.347447/94‑1 (MQ=60)

     CAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCG                                                                                          <  SRR4302533.2849930/94‑1 (MQ=60)

     CAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCG                                                                                          <  SRR4302533.2778589/94‑1 (MQ=60)

      AGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGG                                                                                         >  SRR4302533.435948/1‑94 (MQ=60)

      AGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGG                                                                                         >  SRR4302533.5260268/1‑94 (MQ=60)

      AGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGG                                                                                         >  SRR4302533.3462531/1‑94 (MQ=60)

      AGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGG                                                                                         >  SRR4302533.1516649/1‑94 (MQ=60)

      AGATGCTTTCCCTCCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGG                                                                                         <  SRR4302533.2780277/94‑1 (MQ=60)

       GATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGCATATACGCTGCAAATAGACACCCACTCCGGA                                                                                        >  SRR4302533.3838340/1‑94 (MQ=60)

         TGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCGAATAGACACCCACTCCGGTAC                                                                                      >  SRR4302533.1396185/1‑94 (MQ=60)

         TGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTAC                                                                                      <  SRR4302533.5894860/94‑1 (MQ=60)

           CTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAGTAGACACCCACTCCGATACGC                                                                                    >  SRR4302533.1350590/1‑94 (MQ=60)

           CTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGC                                                                                    >  SRR4302533.1590563/1‑94 (MQ=60)

            TTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCC                                                                                   <  SRR4302533.4568467/94‑1 (MQ=60)

             TTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCA                                                                                  <  SRR4302533.5202221/94‑1 (MQ=60)

              TCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAA                                                                                 <  SRR4302533.1414609/94‑1 (MQ=60)

                CCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACA                                                                               <  SRR4302533.696408/94‑1 (MQ=60)

                CCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACA                                                                               <  SRR4302533.3652927/94‑1 (MQ=60)

                 CTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACAT                                                                              <  SRR4302533.2802865/94‑1 (MQ=60)

                  TGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATT                                                                             <  SRR4302533.2561266/94‑1 (MQ=60)

                  TGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATT                                                                             <  SRR4302533.5550692/94‑1 (MQ=60)

                  TGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATT                                                                             >  SRR4302533.2881100/1‑94 (MQ=60)

                   GCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTG                                                                            >  SRR4302533.3391081/1‑94 (MQ=60)

                   GCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTG                                                                            <  SRR4302533.4803396/94‑1 (MQ=60)

                    CAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGA                                                                           <  SRR4302533.5729910/94‑1 (MQ=60)

                    CAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGCACGCCAACATTGA                                                                           >  SRR4302533.1681298/1‑94 (MQ=60)

                     AACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGAT                                                                          <  SRR4302533.2203275/94‑1 (MQ=60)

                      ACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATG                                                                         >  SRR4302533.3523956/1‑94 (MQ=60)

                      ACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATG                                                                         >  SRR4302533.2496708/1‑94 (MQ=60)

                      ACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATG                                                                         <  SRR4302533.908382/94‑1 (MQ=60)

                          TGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTT                                                                     <  SRR4302533.3880624/94‑1 (MQ=60)

                          TGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTT                                                                     >  SRR4302533.2374983/1‑94 (MQ=60)

                          TGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTT                                                                     <  SRR4302533.1097381/94‑1 (MQ=60)

                            TAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCC                                                                   >  SRR4302533.4620916/1‑94 (MQ=60)

                            TAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCC                                                                   <  SRR4302533.2331300/94‑1 (MQ=60)

                               CAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATC                                                                >  SRR4302533.4517494/1‑94 (MQ=60)

                               CAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATC                                                                <  SRR4302533.4345332/94‑1 (MQ=60)

                                AACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCC                                                               >  SRR4302533.5707264/1‑94 (MQ=60)

                                AACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCC                                                               >  SRR4302533.8454/1‑94 (MQ=60)

                                 ACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACCCCCACTCCGGTACGCCAACATTGATGATTTCCATCCA                                                              <  SRR4302533.1748953/94‑1 (MQ=60)

                                 ACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCA                                                              >  SRR4302533.1425292/1‑94 (MQ=60)

                                  CACGCCGCTTCAATGGCGATTGTTCAGGGACGACGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAG                                                             <  SRR4302533.2517801/94‑1 (MQ=60)

                                  CACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAG                                                             <  SRR4302533.2505043/94‑1 (MQ=60)

                                  CACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAG                                                             >  SRR4302533.4829912/1‑94 (MQ=60)

                                  CACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAG                                                             <  SRR4302533.5518541/94‑1 (MQ=60)

                                  CACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAG                                                             <  SRR4302533.3050914/94‑1 (MQ=60)

                                   ACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGA                                                            <  SRR4302533.5879697/94‑1 (MQ=60)

                                    CGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAA                                                           <  SRR4302533.2227420/94‑1 (MQ=60)

                                    CGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAA                                                           <  SRR4302533.890795/94‑1 (MQ=60)

                                    CGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAA                                                           >  SRR4302533.1432696/1‑94 (MQ=60)

                                    CGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAA                                                           >  SRR4302533.5891992/1‑94 (MQ=60)

                                    CGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAA                                                           <  SRR4302533.1197668/94‑1 (MQ=60)

                                     GCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAAC                                                          >  SRR4302533.365172/1‑94 (MQ=60)

                                     GCCGCCTCAATAGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAAC                                                          <  SRR4302533.959185/94‑1 (MQ=60)

                                        GCCTCACTGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGCCACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCC                                                       <  SRR4302533.383229/94‑1 (MQ=60)

                                        GCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCC                                                       <  SRR4302533.5253145/94‑1 (MQ=60)

                                        GCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCC                                                       <  SRR4302533.5656265/94‑1 (MQ=60)

                                        GCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCC                                                       <  SRR4302533.201727/94‑1 (MQ=60)

                                         CCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCG                                                      >  SRR4302533.1226397/1‑94 (MQ=60)

                                         CCTCAATGGCGATTGTGCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCG                                                      <  SRR4302533.213110/94‑1 (MQ=60)

                                          CTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGC                                                     >  SRR4302533.1405058/1‑94 (MQ=60)

                                           TCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCC                                                    <  SRR4302533.4396867/94‑1 (MQ=60)

                                           TCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCC                                                    <  SRR4302533.190509/94‑1 (MQ=60)

                                            CAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCG                                                   <  SRR4302533.5587357/94‑1 (MQ=60)

                                            CAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCG                                                   >  SRR4302533.4859566/1‑94 (MQ=60)

                                             AATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGA                                                  >  SRR4302533.197730/1‑94 (MQ=60)

                                             AATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGA                                                  <  SRR4302533.5477157/94‑1 (MQ=60)

                                               TGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGCAC                                                >  SRR4302533.2382101/1‑94 (MQ=60)

                                                GGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACC                                               <  SRR4302533.1325423/94‑1 (MQ=60)

                                                 GCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCG                                              <  SRR4302533.4366904/94‑1 (MQ=60)

                                                   GATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGT                                            >  SRR4302533.4425147/1‑94 (MQ=60)

                                                   GATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGT                                            >  SRR4302533.1115218/1‑94 (MQ=60)

                                                   GATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGT                                            <  SRR4302533.5180473/94‑1 (MQ=60)

                                                    ATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTA                                           >  SRR4302533.6329597/1‑94 (MQ=60)

                                                     TTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAG                                          >  SRR4302533.1812392/1‑94 (MQ=60)

                                                     TTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAG                                          >  SRR4302533.4614713/1‑94 (MQ=60)

                                                     TTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAG                                          <  SRR4302533.3097284/94‑1 (MQ=60)

                                                     TTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAG                                          <  SRR4302533.280766/94‑1 (MQ=60)

                                                      TGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTAGGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGC                                         <  SRR4302533.1897407/94‑1 (MQ=60)

                                                      TGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGC                                         >  SRR4302533.1866397/1‑94 (MQ=60)

                                                        TTCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAA                                       >  SRR4302533.1163347/1‑94 (MQ=60)

                                                         TCAGGGACGGCGAATATACGCTGTAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAG                                      <  SRR4302533.1610701/94‑1 (MQ=60)

                                                         TCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAG                                      <  SRR4302533.5518122/94‑1 (MQ=60)

                                                         TCAGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAG                                      <  SRR4302533.188982/94‑1 (MQ=60)

                                                           AGGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCC                                    >  SRR4302533.4276842/1‑94 (MQ=60)

                                                            GGGACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCT                                   <  SRR4302533.3203739/94‑1 (MQ=60)

                                                              GACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGG                                 >  SRR4302533.3740241/1‑94 (MQ=60)

                                                              GACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGG                                 >  SRR4302533.1148241/1‑94 (MQ=60)

                                                               ACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGT                                <  SRR4302533.5518611/94‑1 (MQ=60)

                                                               ACGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGT                                <  SRR4302533.715457/94‑1 (MQ=60)

                                                                CGGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTC                               <  SRR4302533.2643448/94‑1 (MQ=60)

                                                                 GGCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCC                              >  SRR4302533.4013269/1‑94 (MQ=60)

                                                                  GCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCG                             <  SRR4302533.2241910/94‑1 (MQ=60)

                                                                  GCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCG                             >  SRR4302533.6199406/1‑94 (MQ=60)

                                                                  GCGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCG                             <  SRR4302533.4122863/94‑1 (MQ=60)

                                                                   CGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGT                            <  SRR4302533.854310/94‑1 (MQ=60)

                                                                   CGAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGT                            >  SRR4302533.5427111/1‑94 (MQ=60)

                                                                    GAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTG                           <  SRR4302533.4650808/94‑1 (MQ=60)

                                                                    GAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTG                           <  SRR4302533.1755585/94‑1 (MQ=60)

                                                                    CAATATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTG                           <  SRR4302533.863273/94‑1 (MQ=60)

                                                                       TATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAAT                        >  SRR4302533.1035172/1‑94 (MQ=60)

                                                                       TATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAAT                        <  SRR4302533.1109962/94‑1 (MQ=60)

                                                                       TATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAAT                        <  SRR4302533.4762717/94‑1 (MQ=60)

                                                                        ATACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATG                       <  SRR4302533.1465958/94‑1 (MQ=60)

                                                                         TACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGC                      >  SRR4302533.5011999/1‑94 (MQ=60)

                                                                         TACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGC                      >  SRR4302533.2711398/1‑94 (MQ=60)

                                                                         TACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGC                      >  SRR4302533.3518325/1‑94 (MQ=60)

                                                                          ACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCC                     >  SRR4302533.934486/1‑94 (MQ=60)

                                                                          ACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCC                     >  SRR4302533.4441510/1‑94 (MQ=60)

                                                                          ACGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCC                     <  SRR4302533.2482117/94‑1 (MQ=60)

                                                                           CGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCC                    >  SRR4302533.5066828/1‑94 (MQ=60)

                                                                           CGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCC                    >  SRR4302533.1003362/1‑94 (MQ=60)

                                                                           CGCTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCC                    <  SRR4302533.2060727/94‑1 (MQ=60)

                                                                             CTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGA                  >  SRR4302533.3741504/1‑94 (MQ=60)

                                                                             CTGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGA                  >  SRR4302533.5170890/1‑94 (MQ=60)

                                                                              TGCAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGACTGGTCCGTGAATGCCCGAA                 >  SRR4302533.1608678/1‑94 (MQ=60)

                                                                                CAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAAT               >  SRR4302533.3702807/1‑94 (MQ=60)

                                                                                CAAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAAT               <  SRR4302533.4914202/94‑1 (MQ=60)

                                                                                 AAATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATG              <  SRR4302533.2570345/94‑1 (MQ=60)

                                                                                 AAATAGACACCCACTCCGGTACGCCAACATTGATGATTCCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATG              <  SRR4302533.2699113/94‑1 (MQ=60)

                                                                                  AATAGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGG             <  SRR4302533.1569886/94‑1 (MQ=60)

                                                                                     AGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGAT          <  SRR4302533.3251085/94‑1 (MQ=60)

                                                                                     AGACACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGAT          <  SRR4302533.4994265/94‑1 (MQ=60)

                                                                                     AGACACCCACTCCGGTACGCCAACATTGATGATTGCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGAT          <  SRR4302533.2782312/94‑1 (MQ=60)

                                                                                        CACCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGA       >  SRR4302533.6024996/1‑94 (MQ=60)

                                                                                          CCCACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATT     >  SRR4302533.3536064/1‑94 (MQ=60)

                                                                                             ACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTACC  >  SRR4302533.5995889/1‑94 (MQ=60)

                                                                                             ACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTACC  >  SRR4302533.2006655/1‑94 (MQ=60)

                                                                                             ACTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTACC  <  SRR4302533.4048715/94‑1 (MQ=60)

TCAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTACC  >  NC_000913/3735322‑3735508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`