Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,870,765 +G coding (476/1764 nt) dgoA ← 2‑oxo‑3‑deoxygalactonate 6‑phosphate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,870,7641.G100.0% 943.1 / NA 290A159A (GCA→GCC) dgoA2‑oxo‑3‑deoxygalactonate 6‑phosphate aldolase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (138/152);  total (138/152)

Only 100 of 317 total aligned reads displayed.

GCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAA‑GCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCT‑G‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCGATG  >  NC_000913/3870672‑3870851
                                                                                              |                                                                                        
   gTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAA‑GCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCT‑ggc                                                                                       <  2:2020058/93‑3 (MQ=255)
   gTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAA‑GCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCT‑ggc                                                                                       >  2:5410323/1‑91 (MQ=255)
    tGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAA‑GCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCT‑ggcg                                                                                      >  1:3631919/1‑90 (MQ=255)
       gcTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAA‑GCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCa                                                                                   >  1:1059739/1‑93 (MQ=255)
       gcTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAA‑GCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCa                                                                                   <  1:1862761/93‑1 (MQ=255)
        ctctACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAA‑GCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCAc                                                                                  >  1:1505718/1‑93 (MQ=255)
          ctACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAA‑GCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCAcgc                                                                                <  2:2106957/93‑1 (MQ=255)
          ctACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAA‑GCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCAcgc                                                                                <  1:5118028/93‑1 (MQ=255)
           tACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAA‑GCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCAcgcc                                                                               >  1:5368600/1‑93 (MQ=255)
           tACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAA‑GCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCAcgcc                                                                               >  2:3910623/1‑93 (MQ=255)
             cGGATTGCCCGGCGCGATAGAGATCGCTGCCTAA‑GCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCAcgccgc                                                                             <  2:3469116/93‑1 (MQ=255)
                 ttGCCCGGCGCGATAGAGATCGCTGCCTAA‑GCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAAc                                                                         <  1:4220035/93‑1 (MQ=255)
                     ccGGCGCGATAGAGATCGCTGCCTAA‑GCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCa                                                                     <  1:2540303/93‑1 (MQ=255)
                       ggCGCGATAGAGATCGCTGCCTAA‑GCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCaaa                                                                   >  2:3812654/1‑93 (MQ=255)
                        gcgcGATAGAGATCGCTGCCTAA‑GCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAg                                                                  <  1:6077300/93‑1 (MQ=255)
                         cgcgATAGAGATCGCTGCCTAA‑GCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGa                                                                 <  2:1604593/93‑1 (MQ=255)
                         cgcgATAGAGATCGCTGCCTAA‑GCCCGCCCCTGCACAACCGGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGa                                                                 <  1:4020092/93‑1 (MQ=255)
                          gcgATAGAGATCGCTGCCTAA‑GCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGAc                                                                <  1:6176384/93‑1 (MQ=255)
                           cgATAGAGATCGCTGCCTAA‑GCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACt                                                               >  1:915746/1‑93 (MQ=255)
                           cgATAGAGATCGCTGCCTAA‑GCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACt                                                               >  2:4615723/1‑93 (MQ=255)
                             aTAGAGATCGCTGCCTAA‑GCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGc                                                             >  2:2470332/1‑93 (MQ=255)
                             aTAGAGATCGCTGCCTAA‑GCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGc                                                             >  2:1663484/1‑93 (MQ=255)
                              tAGAGATCGCTGCCTAA‑GCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCg                                                            >  2:3748147/1‑93 (MQ=255)
                               agagATCGCTGCCTAA‑GCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGa                                                           <  1:257533/93‑1 (MQ=255)
                               agagATCGCTGCCTAA‑GCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGa                                                           <  2:5958137/93‑1 (MQ=255)
                                 agaTCGCTGCCTAA‑GCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATg                                                         <  1:5537266/93‑1 (MQ=255)
                                 agaTCGCTGCCTAA‑GCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATg                                                         >  2:1984923/1‑93 (MQ=255)
                                  gaTCGCTGCCTAA‑GCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGt                                                        <  1:1299589/93‑1 (MQ=255)
                                   aTCGCTGCCTAA‑GCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTc                                                       >  2:5138770/1‑93 (MQ=255)
                                    tCGCTGCCTAA‑GCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTcc                                                      >  2:4828415/1‑93 (MQ=255)
                                    tCGCTGCCTAA‑GCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTcc                                                      >  2:2271483/1‑93 (MQ=255)
                                    tCGCTGCCTAA‑GCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTcc                                                      <  2:5178442/93‑1 (MQ=255)
                                    tCGCTGCCTAA‑GCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTcc                                                      >  1:3157161/1‑93 (MQ=255)
                                      gCTGCCTAA‑GCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGa                                                    >  1:2238636/1‑93 (MQ=255)
                                      gCTGCCTAA‑GCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGa                                                    <  2:467257/93‑1 (MQ=255)
                                      cactccTAAGGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCAAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCg                                                     <  2:4504851/89‑1 (MQ=255)
                                       cTGCCTAA‑GCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGAt                                                   <  1:4619975/93‑1 (MQ=255)
                                       cTGCCTAA‑GCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGAt                                                   <  2:6224877/93‑1 (MQ=255)
                                        tGCCTAA‑GCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATg                                                  <  2:1880436/93‑1 (MQ=255)
                                        tGCCTAA‑GCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATg                                                  <  1:3826846/93‑1 (MQ=255)
                                         gCCTAA‑GCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATgg                                                 <  1:250598/93‑1 (MQ=255)
                                         accTAA‑GCCCCACCCTGCAAAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATgg                                                 <  2:887544/92‑1 (MQ=255)
                                           cTAA‑GCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCa                                               >  1:1956537/1‑93 (MQ=255)
                                           cTAA‑GCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCa                                               >  2:6086585/1‑93 (MQ=255)
                                           cTAA‑GCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCa                                               >  1:20499/1‑93 (MQ=255)
                                           cTAA‑GCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCa                                               >  1:3549334/1‑93 (MQ=255)
                                            tAA‑ACCAGCCCCTGCACATCCTGCGTCTATCCACTGTGCCAGGTTTTCTGG‑CGTCACGCTGCCAACGGCAAAGACTTCGATGTCCGATGGCaa                                              <  1:5869935/93‑1 (MQ=255)
                                                 cccgcccCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATAcc                                          <  1:3767153/93‑1 (MQ=255)
                                                  ccgcccCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCg                                         >  2:1164491/1‑93 (MQ=255)
                                                   cgcccCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGc                                        <  2:2390576/93‑1 (MQ=255)
                                                     ccccTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCtt                                      >  2:4215964/1‑93 (MQ=255)
                                                      cctggCACACCCTGCTTCTATCCGCTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCttt                                     <  2:4355433/89‑1 (MQ=255)
                                                      cccTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCttt                                     <  2:1359984/93‑1 (MQ=255)
                                                       ccTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCtttt                                    >  2:3603495/1‑93 (MQ=255)
                                                         tGCACAACCTGCGTCTATCCACTGCGTCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTaa                                  <  2:5089414/93‑1 (MQ=255)
                                                         tGCACAACCTGCGTCTATCCACTGCGCGAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTaa                                  <  1:5709092/93‑1 (MQ=255)
                                                         tGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTaa                                  <  2:4925213/93‑1 (MQ=255)
                                                         tGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTaa                                  <  2:5661544/93‑1 (MQ=255)
                                                         tGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTaa                                  <  2:2608380/93‑1 (MQ=255)
                                                          gCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAAc                                 >  2:1460347/1‑93 (MQ=255)
                                                           cacaACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACg                                >  2:3873182/1‑93 (MQ=255)
                                                             caACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCt                              <  2:1007561/93‑1 (MQ=255)
                                                             caACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCt                              >  2:2115350/1‑93 (MQ=255)
                                                             caACCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCt                              >  1:5905003/1‑93 (MQ=255)
                                                              aaCCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCtt                             >  2:3146869/1‑93 (MQ=255)
                                                              aaCCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCtt                             <  1:2759983/93‑1 (MQ=255)
                                                               aCCTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCttt                            <  1:1174003/93‑1 (MQ=255)
                                                                ccTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTg                           <  1:6018058/93‑1 (MQ=255)
                                                                 cTGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGa                          <  1:4453685/93‑1 (MQ=255)
                                                                  tGCTTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGAt                         <  1:5023199/93‑1 (MQ=255)
                                                                  tGCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGAt                         >  1:20730/1‑93 (MQ=255)
                                                                  cgCGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGAt                         <  1:3321308/92‑1 (MQ=255)
                                                                    cGTCTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGt                       >  2:4110652/1‑93 (MQ=255)
                                                                       cTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTAtt                    <  1:5838064/93‑1 (MQ=255)
                                                                       cTATCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTAtt                    <  1:1636968/93‑1 (MQ=255)
                                                                         aTCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGc                  <  2:5996477/93‑1 (MQ=255)
                                                                         aTCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGc                  >  2:721957/1‑93 (MQ=255)
                                                                         aTCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGc                  <  1:479022/93‑1 (MQ=255)
                                                                          tCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCg                 >  1:2932745/1‑93 (MQ=255)
                                                                          tCCACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCg                 >  1:1475567/1‑93 (MQ=255)
                                                                           ccACTGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCgg                <  1:799843/93‑1 (MQ=255)
                                                                               tGCGCCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCa            <  1:4507358/93‑1 (MQ=255)
                                                                                 cgcCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCaaa          <  2:669200/93‑1 (MQ=255)
                                                                                  gcCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCaaaa         <  2:3980484/93‑1 (MQ=255)
                                                                                  gcCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCaaaa         >  1:5498393/1‑93 (MQ=255)
                                                                                  gcCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCaaaa         <  2:1803366/93‑1 (MQ=255)
                                                                                  gcCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCaaaa         <  2:2232796/93‑1 (MQ=255)
                                                                                  gcCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCaaaa         <  2:5891626/93‑1 (MQ=255)
                                                                                  gcCAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCaaaa         <  1:1773375/93‑1 (MQ=255)
                                                                                    cAGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGc       <  2:5734536/93‑1 (MQ=255)
                                                                                     atgTTTTCT‑GTCGCCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGcc      <  2:1238404/91‑1 (MQ=255)
                                                                                     aGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGcc      <  2:5297245/93‑1 (MQ=255)
                                                                                     aGGTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGcc      <  2:5389534/93‑1 (MQ=255)
                                                                                       gTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCGa    >  1:4101669/1‑93 (MQ=255)
                                                                                       gTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCGa    >  2:112928/1‑93 (MQ=255)
                                                                                       gTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCGa    <  1:600130/93‑1 (MQ=255)
                                                                                       gTTTTCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCGa    <  2:2793641/93‑1 (MQ=255)
                                                                                        ttttCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCGAt   <  2:6185159/93‑1 (MQ=255)
                                                                                        ttttCTGG‑CGGCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCGAt   <  2:1400248/93‑1 (MQ=255)
                                                                                         tttCTGG‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCGATg  <  1:2359796/93‑1 (MQ=255)
                                                                                              |                                                                                        
GCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAA‑GCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCT‑G‑CGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCGATG  >  NC_000913/3870672‑3870851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCT‑GCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCGATGACGGAA  >  NC_000913/3870672‑3870857
                                                                                              |                                                                                            
GCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCT‑G                                                                                              >  SRR4302533.451591/1‑94 (MQ=60)
GCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCT‑G                                                                                              <  SRR4302533.3600127/94‑1 (MQ=60)
   GTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCT‑Ggcg                                                                                           >  SRR4302533.3654263/1‑91 (MQ=60)
   GTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCT‑Ggcg                                                                                           <  SRR4302533.6050424/94‑4 (MQ=60)
    TGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGT                                                                                           >  SRR4302533.1094910/1‑94 (MQ=60)
      CGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCA                                                                                         >  SRR4302533.1065969/1‑94 (MQ=60)
      CGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCA                                                                                         <  SRR4302533.5486417/94‑1 (MQ=60)
      CGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCA                                                                                         >  SRR4302533.5973117/1‑94 (MQ=60)
      CGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCA                                                                                         >  SRR4302533.6188733/1‑94 (MQ=60)
      CGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCA                                                                                         <  SRR4302533.1002504/94‑1 (MQ=60)
       GCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCAC                                                                                        >  SRR4302533.1514799/1‑94 (MQ=60)
       GCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCAC                                                                                        <  SRR4302533.1874121/94‑1 (MQ=60)
        CTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACG                                                                                       >  SRR4302533.5863977/1‑94 (MQ=60)
          CTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCC                                                                                     >  SRR4302533.2587599/1‑94 (MQ=60)
          CTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCC                                                                                     <  SRR4302533.5148952/94‑1 (MQ=60)
          CTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCC                                                                                     >  SRR4302533.5400850/1‑94 (MQ=60)
          CTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCC                                                                                     <  SRR4302533.120003/94‑1 (MQ=60)
           TACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCG                                                                                    >  SRR4302533.4503667/1‑94 (MQ=60)
             CGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCC                                                                                  >  SRR4302533.494927/1‑94 (MQ=60)
              GGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCA                                                                                 >  SRR4302533.1814343/1‑94 (MQ=60)
               GATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAA                                                                                >  SRR4302533.2076209/1‑94 (MQ=60)
                ATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAAC                                                                               >  SRR4302533.3686303/1‑94 (MQ=60)
                ATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAAC                                                                               <  SRR4302533.6087347/94‑1 (MQ=60)
                 TTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACG                                                                              <  SRR4302533.4245552/94‑1 (MQ=60)
                  TGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGG                                                                             <  SRR4302533.2353574/94‑1 (MQ=60)
                     CCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAA                                                                          <  SRR4302533.2549139/94‑1 (MQ=60)
                     CCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAA                                                                          <  SRR4302533.2555940/94‑1 (MQ=60)
                       GGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAG                                                                        <  SRR4302533.552381/94‑1 (MQ=60)
                        GCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGA                                                                       <  SRR4302533.6114057/94‑1 (MQ=60)
                         CGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGAC                                                                      >  SRR4302533.2336909/1‑94 (MQ=60)
                         CGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGAC                                                                      >  SRR4302533.40884/1‑94 (MQ=60)
                         CGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGAC                                                                      <  SRR4302533.2063213/94‑1 (MQ=60)
                         CGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGAC                                                                      >  SRR4302533.812389/1‑94 (MQ=60)
                         CGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCGGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGAC                                                                      <  SRR4302533.4044573/94‑1 (MQ=60)
                          GCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACT                                                                     <  SRR4302533.3228245/94‑1 (MQ=60)
                          GCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACT                                                                     >  SRR4302533.921127/1‑94 (MQ=60)
                          GCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACT                                                                     <  SRR4302533.6213493/94‑1 (MQ=60)
                            GATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTCC                                                                   >  SRR4302533.2255061/1‑94 (MQ=60)
                             ATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCGAAGACTGCG                                                                  >  SRR4302533.2226711/1‑94 (MQ=60)
                              TAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGA                                                                 >  SRR4302533.2562942/1‑94 (MQ=60)
                               AGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGAT                                                                <  SRR4302533.4616455/94‑1 (MQ=60)
                               AGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGAT                                                                >  SRR4302533.3572919/1‑94 (MQ=60)
                               AGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGAT                                                                <  SRR4302533.259099/94‑1 (MQ=60)
                               AGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGAT                                                                <  SRR4302533.589751/94‑1 (MQ=60)
                                GAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATG                                                               <  SRR4302533.2443676/94‑1 (MQ=60)
                                GAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATG                                                               <  SRR4302533.865526/94‑1 (MQ=60)
                                 AGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGT                                                              <  SRR4302533.5570706/94‑1 (MQ=60)
                                 AGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGT                                                              >  SRR4302533.1799219/1‑94 (MQ=60)
                                  GATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTC                                                             <  SRR4302533.1307410/94‑1 (MQ=60)
                                   ATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCC                                                            <  SRR4302533.241387/94‑1 (MQ=60)
                                   ATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCC                                                            >  SRR4302533.370585/1‑94 (MQ=60)
                                   ATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCC                                                            >  SRR4302533.4171907/1‑94 (MQ=60)
                                   ATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCC                                                            >  SRR4302533.3176581/1‑94 (MQ=60)
                                    tcgcgcccaagcCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCG                                                           <  SRR4302533.4421213/82‑1 (MQ=60)
                                     CGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGA                                                          <  SRR4302533.142866/94‑1 (MQ=60)
                                     CGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGA                                                          >  SRR4302533.2252470/1‑94 (MQ=60)
                                      GCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGAT                                                         <  SRR4302533.2627146/94‑1 (MQ=60)
                                      GCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGAT                                                         <  SRR4302533.6067922/94‑1 (MQ=60)
                                       CTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATG                                                        <  SRR4302533.4647974/94‑1 (MQ=60)
                                       CTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATG                                                        >  SRR4302533.510118/1‑94 (MQ=60)
                                       CTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATG                                                        >  SRR4302533.4660095/1‑94 (MQ=60)
                                       CTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATG                                                        >  SRR4302533.988520/1‑94 (MQ=60)
                                       CTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATG                                                        >  SRR4302533.2713075/1‑94 (MQ=60)
                                       CTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATG                                                        <  SRR4302533.1443310/94‑1 (MQ=60)
                                       CTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATG                                                        >  SRR4302533.3011403/1‑94 (MQ=60)
                                       CTCCCTAAGCCCCCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATG                                                        <  SRR4302533.1570160/94‑1 (MQ=60)
                                        TGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGG                                                       <  SRR4302533.2096783/94‑1 (MQ=60)
                                        TGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGG                                                       <  SRR4302533.3850240/94‑1 (MQ=60)
                                        TGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGG                                                       <  SRR4302533.5538198/94‑1 (MQ=60)
                                         GCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGC                                                      >  SRR4302533.3493376/1‑94 (MQ=60)
                                         GCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGC                                                      <  SRR4302533.252144/94‑1 (MQ=60)
                                         GCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGC                                                      <  SRR4302533.5692518/94‑1 (MQ=60)
                                          CCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCA                                                     >  SRR4302533.1968474/1‑94 (MQ=60)
                                          CCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCA                                                     >  SRR4302533.3571203/1‑94 (MQ=60)
                                          CCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCA                                                     >  SRR4302533.20636/1‑94 (MQ=60)
                                           CTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAA                                                    <  SRR4302533.6078818/94‑1 (MQ=60)
                                             AAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATA                                                  >  SRR4302533.121810/1‑94 (MQ=60)
                                              AGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATAC                                                 <  SRR4302533.2883836/94‑1 (MQ=60)
                                               GCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACC                                                >  SRR4302533.3892218/1‑94 (MQ=60)
                                                CCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCG                                               <  SRR4302533.3790250/94‑1 (MQ=60)
                                                CCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCG                                               >  SRR4302533.4585928/1‑94 (MQ=60)
                                                 CCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGC                                              >  SRR4302533.4356163/1‑94 (MQ=60)
                                                  CGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCT                                             >  SRR4302533.3957374/1‑94 (MQ=60)
                                                  CGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCT                                             >  SRR4302533.1966614/1‑94 (MQ=60)
                                                  CGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCT                                             <  SRR4302533.400745/94‑1 (MQ=60)
                                                  CGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCT                                             >  SRR4302533.3053798/1‑94 (MQ=60)
                                                   CCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTT                                            <  SRR4302533.6166757/94‑1 (MQ=60)
                                                    CCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTT                                           >  SRR4302533.2929411/1‑94 (MQ=60)
                                                    CCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTT                                           >  SRR4302533.5188411/1‑94 (MQ=60)
                                                     CCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTT                                          >  SRR4302533.4141129/1‑94 (MQ=60)
                                                     CCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTGTCTGGCGTCACGCCGCCACCGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTT                                          <  SRR4302533.1725928/94‑1 (MQ=60)
                                                      CCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTA                                         <  SRR4302533.5119150/94‑1 (MQ=60)
                                                       CTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAA                                        <  SRR4302533.5563026/94‑1 (MQ=60)
                                                        TGCACAACCTGCGTCTATCCACTGCGCGAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAAC                                       <  SRR4302533.5743574/94‑1 (MQ=60)
                                                        TGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAAC                                       >  SRR4302533.5311134/1‑94 (MQ=60)
                                                        TGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAAC                                       <  SRR4302533.1702343/94‑1 (MQ=60)
                                                          CACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGC                                     >  SRR4302533.643794/1‑94 (MQ=60)
                                                           ACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCT                                    <  SRR4302533.5418797/94‑1 (MQ=60)
                                                           ACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCT                                    <  SRR4302533.323860/94‑1 (MQ=60)
                                                           ACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCT                                    >  SRR4302533.5940665/1‑94 (MQ=60)
                                                           ACAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCT                                    <  SRR4302533.199200/94‑1 (MQ=60)
                                                            CAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTT                                   >  SRR4302533.1128272/1‑94 (MQ=60)
                                                            CAACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTT                                   >  SRR4302533.1993342/1‑94 (MQ=60)
                                                             AACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTT                                  <  SRR4302533.2776899/94‑1 (MQ=60)
                                                              ACCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTG                                 <  SRR4302533.1181050/94‑1 (MQ=60)
                                                               CCTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGA                                <  SRR4302533.6054526/94‑1 (MQ=60)
                                                                CTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGAT                               >  SRR4302533.5671954/1‑94 (MQ=60)
                                                                CTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGAT                               <  SRR4302533.1659753/94‑1 (MQ=60)
                                                                CTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGAT                               <  SRR4302533.4480614/94‑1 (MQ=60)
                                                                CTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGAT                               >  SRR4302533.3794305/1‑94 (MQ=60)
                                                                CTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGAT                               >  SRR4302533.3568940/1‑94 (MQ=60)
                                                                CTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGAT                               >  SRR4302533.20867/1‑94 (MQ=60)
                                                                CTGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGAT                               <  SRR4302533.947217/94‑1 (MQ=60)
                                                                 TGCTTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATG                              <  SRR4302533.5053620/94‑1 (MQ=60)
                                                                 CGCGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATG                              <  SRR4302533.3341820/94‑1 (MQ=60)
                                                                   CGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTA                            >  SRR4302533.1498742/1‑94 (MQ=60)
                                                                   CGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTA                            <  SRR4302533.6039687/94‑1 (MQ=60)
                                                                   CGTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTA                            <  SRR4302533.2554623/94‑1 (MQ=60)
                                                                    GTCTATCCACTGCGCGAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTAT                           <  SRR4302533.5898374/94‑1 (MQ=60)
                                                                    GTCTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTAT                           >  SRR4302533.4706871/1‑94 (MQ=60)
                                                                      CTATCCACTGCGCGAGGTGTGCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTG                         <  SRR4302533.3804012/94‑1 (MQ=60)
                                                                      CTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTG                         <  SRR4302533.4745249/94‑1 (MQ=60)
                                                                      CTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTG                         <  SRR4302533.5873361/94‑1 (MQ=60)
                                                                      CTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTG                         >  SRR4302533.4254056/1‑94 (MQ=60)
                                                                      CTATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTG                         <  SRR4302533.1646881/94‑1 (MQ=60)
                                                                        ATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCG                       <  SRR4302533.481884/94‑1 (MQ=60)
                                                                        ATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCG                       >  SRR4302533.2950680/1‑94 (MQ=60)
                                                                        ATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCG                       >  SRR4302533.1484487/1‑94 (MQ=60)
                                                                        ATCCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCG                       >  SRR4302533.4926900/1‑94 (MQ=60)
                                                                          CCACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGA                     <  SRR4302533.804548/94‑1 (MQ=60)
                                                                            ACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACC                   <  SRR4302533.1923233/94‑1 (MQ=60)
                                                                            ACTGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACC                   >  SRR4302533.3053174/1‑94 (MQ=60)
                                                                              TGCGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAA                 <  SRR4302533.4534656/94‑1 (MQ=60)
                                                                                CGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAA               >  SRR4302533.5916611/1‑94 (MQ=60)
                                                                                CGCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAA               >  SRR4302533.5531557/1‑94 (MQ=60)
                                                                                 GCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAG              <  SRR4302533.1593976/94‑1 (MQ=60)
                                                                                 GCCAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAG              <  SRR4302533.1784176/94‑1 (MQ=60)
                                                                                 CCCAGGTTTTCTGGCGTCTCGCCGCGAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAG              <  SRR4302533.4074144/94‑1 (MQ=60)
                                                                                   CAGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCC            >  SRR4302533.5924709/1‑94 (MQ=60)
                                                                                    AGGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCG           <  SRR4302533.727053/94‑1 (MQ=60)
                                                                                     GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCGA          >  SRR4302533.4126627/1‑94 (MQ=60)
                                                                                      GTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCGAT         <  SRR4302533.5648162/94‑1 (MQ=60)
                                                                                      GTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCGAT         <  SRR4302533.603660/94‑1 (MQ=60)
                                                                                       TTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCGATG        <  SRR4302533.578683/94‑1 (MQ=60)
                                                                                        TTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCGATGA       >  SRR4302533.6310364/1‑94 (MQ=60)
                                                                                        TTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCGATGA       <  SRR4302533.2374379/94‑1 (MQ=60)
                                                                                         TTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCGATGAC      <  SRR4302533.1699183/94‑1 (MQ=60)
                                                                                          TCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCGATGACG     <  SRR4302533.1337903/94‑1 (MQ=60)
                                                                                           ctgGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCGATGACGG    <  SRR4302533.2639880/91‑1 (MQ=60)
                                                                                            G‑GCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCGATGACGGAA  <  SRR4302533.1196868/94‑1 (MQ=60)
                                                                                              |                                                                                            
GCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCT‑GCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCGATGACGGAA  >  NC_000913/3870672‑3870857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑