Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,897,198	(C)_5→6	coding (1041/1203 nt)	yieL ←	putative xylanase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,897,193	1	.	C	100.0%	643.3 / NA	197	D349G (GAT→GGT)	yieL	putative xylanase
Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (127/70); total (127/70)

Only 100 of 219 total aligned reads displayed.

GACCCGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑T‑CCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGG  >  NC_000913/3897101‑3897280

gACCCGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑t                                                                                          >  2:2059498/1‑93 (MQ=255)

    cGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑T‑cctc                                                                                     >  1:3733573/1‑91 (MQ=255)

    cGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑T‑cccc                                                                                     >  1:3420242/1‑93 (MQ=255)

     ggATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑T‑ccccc                                                                                    <  2:3832911/93‑1 (MQ=255)

     ggATATTCCTCGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑T‑ccccc                                                                                    <  1:3496740/93‑1 (MQ=255)

        tatTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑T‑ccccccac                                                                                 <  2:1946410/93‑4 (MQ=255)

        tatTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑T‑ccccccac                                                                                 >  1:2121573/1‑90 (MQ=255)

         atTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑T‑ccccccaca                                                                                <  2:120441/93‑5 (MQ=255)

         atTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑T‑ccccccaca                                                                                >  1:5189432/1‑89 (MQ=255)

           tCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAAc                                                                               >  1:2644748/1‑93 (MQ=255)

           tCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAAc                                                                               <  1:5204737/93‑1 (MQ=255)

            ccTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAAcc                                                                              >  1:2732929/1‑93 (MQ=255)

            ccTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAAcc                                                                              >  2:3941276/1‑93 (MQ=255)

            ccTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAAcc                                                                              >  1:5416528/1‑93 (MQ=255)

             cTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACca                                                                             >  1:1566261/1‑93 (MQ=255)

              tGGTAATCAAAATTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACcac                                                                            <  2:3338645/93‑1 (MQ=255)

               ggTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACcaca                                                                           <  2:5240484/93‑1 (MQ=255)

                gTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAg                                                                          <  2:1763872/93‑1 (MQ=255)

                 tAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGt                                                                         >  2:4080225/1‑93 (MQ=255)

                 tAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGt                                                                         >  1:3148446/1‑93 (MQ=255)

                 tAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGt                                                                         >  2:2212783/1‑93 (MQ=255)

                  aaTCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTa                                                                        >  1:2054848/1‑93 (MQ=255)

                   aTCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTaa                                                                       >  1:1725820/1‑93 (MQ=255)

                   aTCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTaa                                                                       <  2:4704365/93‑1 (MQ=255)

                     cAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTaaaa                                                                     <  2:3262723/93‑1 (MQ=255)

                      aaaGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAAt                                                                    >  1:4306045/1‑93 (MQ=255)

                      aaaGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAAt                                                                    >  1:4418799/1‑93 (MQ=255)

                      aaaGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAAt                                                                    >  2:6243146/1‑93 (MQ=255)

                      aaaGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAAGAAAAt                                                                    >  2:4789656/1‑93 (MQ=255)

                       aaGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAAtt                                                                   >  1:3909099/1‑93 (MQ=255)

                       aaGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAAtt                                                                   >  2:5807566/1‑93 (MQ=255)

                       aaGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAAtt                                                                   >  2:5998178/1‑93 (MQ=255)

                       aaGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAAtt                                                                   >  1:5722144/1‑93 (MQ=255)

                        aGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTa                                                                  <  1:4234182/93‑1 (MQ=255)

                          ttAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACCACCACCGTAAAATTACg                                                                >  2:5211643/1‑93 (MQ=255)

                           tAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGt                                                               >  2:1885396/1‑93 (MQ=255)

                           tAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGt                                                               <  1:675642/93‑1 (MQ=255)

                            aaTTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTa                                                              <  2:3555196/93‑1 (MQ=255)

                                tttttCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTg                                                          <  2:2695612/93‑1 (MQ=255)

                                  tttCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCt                                                        <  2:3157883/93‑1 (MQ=255)

                                  tttCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCt                                                        <  2:4328433/93‑1 (MQ=255)

                                   ttCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTg                                                       >  2:5589579/1‑93 (MQ=255)

                                    tCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTgg                                                      <  1:5609051/93‑1 (MQ=255)

                                    tCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTgg                                                      >  2:2827865/1‑93 (MQ=255)

                                    tCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTgg                                                      <  1:4328560/93‑1 (MQ=255)

                                     cTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGt                                                     >  2:4288560/1‑93 (MQ=255)

                                      tGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCATAGTAAAATTACGTAGTTGCTGGtt                                                    <  2:4131636/93‑1 (MQ=255)

                                      tGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGtt                                                    <  1:5791035/93‑1 (MQ=255)

                                         tCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGAt                                                 >  1:3337305/1‑93 (MQ=255)

                                         tCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGAt                                                 >  2:1513315/1‑93 (MQ=255)

                                          cAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATa                                                <  1:4765806/93‑1 (MQ=255)

                                          cAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATa                                                >  2:1003209/1‑93 (MQ=255)

                                          cAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATa                                                <  2:4430009/93‑1 (MQ=255)

                                          cAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATa                                                <  2:5315232/93‑1 (MQ=255)

                                          cAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATa                                                >  1:2975320/1‑93 (MQ=255)

                                           aaGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAg                                               <  1:5286277/93‑1 (MQ=255)

                                             gCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCt                                             >  1:1424800/1‑93 (MQ=255)

                                               tCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGc                                           >  1:2691146/1‑93 (MQ=255)

                                                cAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACTTCTTTACC‑CCCCCACAACCGCAGTAAAATTACGTAGTTGCTGGTTGATAGCTGcc                                          <  2:5391528/93‑1 (MQ=255)

                                                cAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGcc                                          >  2:2521556/1‑93 (MQ=255)

                                                cAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGCTGCTGGTTGATAGCTGcc                                          >  2:882343/1‑93 (MQ=255)

                                                  gTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCgg                                        >  2:5859922/1‑93 (MQ=255)

                                                   ttttCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGa                                       >  2:704289/1‑93 (MQ=255)

                                                   ttttCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGa                                       >  1:6025472/1‑93 (MQ=255)

                                                        aGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGtt                                  >  1:17788/1‑93 (MQ=255)

                                                         gCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTc                                 >  1:77785/1‑93 (MQ=255)

                                                         gCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTc                                 <  1:5957564/93‑1 (MQ=255)

                                                          cccGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCa                                >  2:1559794/1‑93 (MQ=255)

                                                           ccGGCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAg                               >  2:4623381/1‑93 (MQ=255)

                                                             ggCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTc                             >  1:1628786/1‑93 (MQ=255)

                                                              gCGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCg                            <  2:2725783/93‑1 (MQ=255)

                                                               cGATATCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGGAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCgg                           <  1:1049220/93‑1 (MQ=255)

                                                                 atatCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGc                         >  2:633083/1‑93 (MQ=255)

                                                                 atatCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGc                         <  2:4214497/93‑1 (MQ=255)

                                                                  tatCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGcc                        >  1:4163884/1‑93 (MQ=255)

                                                                  tatCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGcc                        >  2:174176/1‑93 (MQ=255)

                                                                   atCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACTGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCcg                       <  2:5630633/93‑1 (MQ=255)

                                                                   atCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCcg                       >  2:5068302/1‑93 (MQ=255)

                                                                    tCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCcgc                      >  1:942968/1‑93 (MQ=255)

                                                                    tCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCcgc                      >  1:2911583/1‑93 (MQ=255)

                                                                    tCCTTGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCcgc                      >  1:4043697/1‑93 (MQ=255)

                                                                       ttGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGac                   <  2:5724323/93‑1 (MQ=255)

                                                                       ttGCCGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGGAGGTGCTGGTTGATAGCTGCCGGATCGCTCAGTCGCGCcgcgca                   >  2:63028/1‑91 (MQ=255)

                                                                           cGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCt               >  1:2834287/1‑93 (MQ=255)

                                                                           cGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCt               >  2:2925304/1‑93 (MQ=255)

                                                                           cGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCt               >  2:1075663/1‑93 (MQ=255)

                                                                           cGGTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGAAACCt               >  1:4888154/1‑93 (MQ=255)

                                                                            ggTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCtt              <  2:3452393/93‑1 (MQ=255)

                                                                             gTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCtta             >  2:1354309/1‑92 (MQ=255)

                                                                             gTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTc             >  2:5025063/1‑93 (MQ=255)

                                                                             gTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTc             <  2:4807274/93‑1 (MQ=255)

                                                                             gTTACGACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTc             >  1:3410252/1‑93 (MQ=255)

                                                                                  gACATCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCg        >  2:3801219/1‑93 (MQ=255)

                                                                                     aTCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTa     >  1:5985348/1‑93 (MQ=255)

                                                                                      tCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTAc    >  1:3536102/1‑93 (MQ=255)

                                                                                      tCTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTAc    >  1:2322631/1‑93 (MQ=255)

                                                                                       cTTTA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACg   <  1:3014156/93‑1 (MQ=255)

                                                                                        tttA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACgg  <  1:5557543/93‑1 (MQ=255)

                                                                                        tttA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACgg  <  2:4381442/93‑1 (MQ=255)

                                                                                        tttA‑TCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACgg  >  1:5423013/1‑93 (MQ=255)

GACCCGGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTA‑T‑CCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGG  >  NC_000913/3897101‑3897280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTAT‑CCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTA  >  NC_000913/3897106‑3897291

GGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTAT‑CCCCCC                                                                                              >  SRR4302533.3468656/1‑94 (MQ=60)

GGATATTCCTCGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTAT‑CCCCCC                                                                                              <  SRR4302533.3518302/94‑1 (MQ=60)

  ATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTAT‑CCCCCcac                                                                                            >  SRR4302533.2134651/1‑91 (MQ=60)

  ATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTAT‑CCCCCcac                                                                                            >  SRR4302533.3967788/1‑91 (MQ=60)

   TATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACA                                                                                            >  SRR4302533.5220746/1‑94 (MQ=60)

   TATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACA                                                                                            <  SRR4302533.4951579/94‑1 (MQ=60)

     TTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAAC                                                                                          >  SRR4302533.2660999/1‑94 (MQ=60)

     TTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAAC                                                                                          <  SRR4302533.4690156/94‑1 (MQ=60)

     TTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAAC                                                                                          >  SRR4302533.6005168/1‑94 (MQ=60)

      TCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACC                                                                                         >  SRR4302533.5449109/1‑94 (MQ=60)

      TCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACC                                                                                         <  SRR4302533.5236098/94‑1 (MQ=60)

      TCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACC                                                                                         >  SRR4302533.2749694/1‑94 (MQ=60)

       CCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCA                                                                                        >  SRR4302533.1575692/1‑94 (MQ=60)

           GTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGT                                                                                    >  SRR4302533.3167802/1‑94 (MQ=60)

            TAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTA                                                                                   >  SRR4302533.2067523/1‑94 (MQ=60)

             AATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAA                                                                                  >  SRR4302533.1736293/1‑94 (MQ=60)

             AATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAA                                                                                  >  SRR4302533.777946/1‑94 (MQ=60)

              ATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAA                                                                                 <  SRR4302533.3855674/94‑1 (MQ=60)

               TCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAA                                                                                >  SRR4302533.808484/1‑94 (MQ=60)

                CAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAAT                                                                               >  SRR4302533.4445514/1‑94 (MQ=60)

                CAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAAT                                                                               >  SRR4302533.4331954/1‑94 (MQ=60)

                 AAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATT                                                                              >  SRR4302533.3932975/1‑94 (MQ=60)

                 AAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATT                                                                              <  SRR4302533.212230/94‑1 (MQ=60)

                 AAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATT                                                                              >  SRR4302533.1653405/1‑94 (MQ=60)

                 AAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATT                                                                              >  SRR4302533.5756695/1‑94 (MQ=60)

                  AAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTA                                                                             >  SRR4302533.4886495/1‑94 (MQ=60)

                  AAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTA                                                                             <  SRR4302533.6052595/94‑1 (MQ=60)

                   AGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTAC                                                                            <  SRR4302533.4259770/94‑1 (MQ=60)

                     TTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGT                                                                          >  SRR4302533.2759234/1‑94 (MQ=60)

                      TAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTA                                                                         <  SRR4302533.679634/94‑1 (MQ=60)

                          TTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTG                                                                     <  SRR4302533.5242848/94‑1 (MQ=60)

                           TTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGC                                                                    >  SRR4302533.5558048/1‑94 (MQ=60)

                            TTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCT                                                                   <  SRR4302533.5455119/94‑1 (MQ=60)

                            TTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCT                                                                   >  SRR4302533.356029/1‑94 (MQ=60)

                             TTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTG                                                                  >  SRR4302533.4190833/1‑94 (MQ=60)

                              TTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGG                                                                 <  SRR4302533.1932040/94‑1 (MQ=60)

                               TCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGT                                                                <  SRR4302533.311406/94‑1 (MQ=60)

                               TCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGT                                                                <  SRR4302533.4354638/94‑1 (MQ=60)

                               TCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGT                                                                <  SRR4302533.5642949/94‑1 (MQ=60)

                               TCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGT                                                                >  SRR4302533.2710255/1‑94 (MQ=60)

                                CTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTT                                                               >  SRR4302533.1851649/1‑94 (MQ=60)

                                 TGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTTCTGGTTG                                                              <  SRR4302533.3649015/94‑1 (MQ=60)

                                 TGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTG                                                              <  SRR4302533.5826021/94‑1 (MQ=60)

                                 TGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTG                                                              >  SRR4302533.3028752/1‑94 (MQ=60)

                                 TGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTG                                                              >  SRR4302533.5107470/1‑94 (MQ=60)

                                   CTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGAT                                                            >  SRR4302533.3357928/1‑94 (MQ=60)

                                   CTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGAT                                                            >  SRR4302533.6214233/1‑94 (MQ=60)

                                    TCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATA                                                           >  SRR4302533.2993526/1‑94 (MQ=60)

                                     CAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAG                                                          <  SRR4302533.4794705/94‑1 (MQ=60)

                                      AAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGC                                                         <  SRR4302533.5317941/94‑1 (MQ=60)

                                      AAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGC                                                         >  SRR4302533.1125376/1‑94 (MQ=60)

                                       AGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCT                                                        <  SRR4302533.4311416/94‑1 (MQ=60)

                                       AGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCT                                                        >  SRR4302533.1433418/1‑94 (MQ=60)

                                        GCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTG                                                       >  SRR4302533.5603708/1‑94 (MQ=60)

                                        GCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTG                                                       <  SRR4302533.341012/94‑1 (MQ=60)

                                        GCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTG                                                       >  SRR4302533.5514819/1‑94 (MQ=60)

                                         CTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGC                                                      >  SRR4302533.2522793/1‑94 (MQ=60)

                                         CTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGC                                                      >  SRR4302533.2707642/1‑94 (MQ=60)

                                         CTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGC                                                      >  SRR4302533.5224308/1‑94 (MQ=60)

                                          TCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCC                                                     >  SRR4302533.2738592/1‑94 (MQ=60)

                                             GTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGT                                                  >  SRR4302533.5333294/1‑94 (MQ=60)

                                             GTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGA                                                  >  SRR4302533.6061983/1‑94 (MQ=60)

                                              TTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGAT                                                 <  SRR4302533.4360989/94‑1 (MQ=60)

                                                  CAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTT                                             >  SRR4302533.17910/1‑94 (MQ=60)

                                                   AGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTC                                            >  SRR4302533.78286/1‑94 (MQ=60)

                                                   AGCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTC                                            <  SRR4302533.5848123/94‑1 (MQ=60)

                                                    GCCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCA                                           <  SRR4302533.5993600/94‑1 (MQ=60)

                                                     CCCGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAG                                          >  SRR4302533.1589420/1‑94 (MQ=60)

                                                       CGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTC                                        >  SRR4302533.1638651/1‑94 (MQ=60)

                                                       CGGCGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTC                                        >  SRR4302533.3541570/1‑94 (MQ=60)

                                                          CGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGG                                     <  SRR4302533.2927523/94‑1 (MQ=60)

                                                          CGATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGGAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGG                                     <  SRR4302533.1055388/94‑1 (MQ=60)

                                                           GATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGC                                    <  SRR4302533.5690819/94‑1 (MQ=60)

                                                           GATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGC                                    >  SRR4302533.3326910/1‑94 (MQ=60)

                                                           GATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGC                                    <  SRR4302533.1533535/94‑1 (MQ=60)

                                                            ATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCC                                   >  SRR4302533.4189110/1‑94 (MQ=60)

                                                            ATATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCC                                   >  SRR4302533.409179/1‑94 (MQ=60)

                                                              ATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGC                                 >  SRR4302533.1740914/1‑94 (MQ=60)

                                                              ATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGC                                 >  SRR4302533.948514/1‑94 (MQ=60)

                                                              ATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGC                                 >  SRR4302533.4068310/1‑94 (MQ=60)

                                                              ATCCTTGCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGC                                 >  SRR4302533.2929454/1‑94 (MQ=60)

                                                                    GCCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACC                           <  SRR4302533.4784675/94‑1 (MQ=60)

                                                                     CCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCT                          >  SRR4302533.2851734/1‑94 (MQ=60)

                                                                     CCGGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGAAACCT                          >  SRR4302533.4917771/1‑94 (MQ=60)

                                                                       GGTTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTC                        >  SRR4302533.3431316/1‑94 (MQ=60)

                                                                         TTACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCAT                      >  SRR4302533.6038978/1‑94 (MQ=60)

                                                                          TACGACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATC                     <  SRR4302533.5823542/94‑1 (MQ=60)

                                                                             GACATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGG                  <  SRR4302533.3438558/94‑1 (MQ=60)

                                                                               CATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTA                >  SRR4302533.6021596/1‑94 (MQ=60)

                                                                                ATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTAC               >  SRR4302533.3557895/1‑94 (MQ=60)

                                                                                ATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTAC               >  SRR4302533.2337005/1‑94 (MQ=60)

                                                                                ATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTAC               <  SRR4302533.4317116/94‑1 (MQ=60)

                                                                                ATCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTAC               <  SRR4302533.2932753/94‑1 (MQ=60)

                                                                                 tctttaccCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACG              <  SRR4302533.5264671/86‑1 (MQ=60)

                                                                                 TCTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACG              <  SRR4302533.2662414/94‑1 (MQ=60)

                                                                                  CTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGG             <  SRR4302533.3032591/94‑1 (MQ=60)

                                                                                  CTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGG             >  SRR4302533.4344264/1‑94 (MQ=60)

                                                                                  CTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGG             >  SRR4302533.2155717/1‑94 (MQ=60)

                                                                                  CTTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGG             >  SRR4302533.5455646/1‑94 (MQ=60)

                                                                                   TTTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGT            <  SRR4302533.5591130/94‑1 (MQ=60)

                                                                                    TTATCCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTT           <  SRR4302533.5471604/94‑1 (MQ=60)

                                                                                       tcCCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTC        <  SRR4302533.5116227/92‑1 (MQ=60)

                                                                                       C‑CCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCG       >  SRR4302533.1661473/1‑94 (MQ=60)

                                                                                       C‑CCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCG       >  SRR4302533.77529/1‑94 (MQ=60)

                                                                                         CCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGT      >  SRR4302533.1643933/1‑94 (MQ=60)

                                                                                          CCCCACAACCACAGTAAATTTACGTATTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACTCCTTCATCCGGTACGGTTGTCGTG     <  SRR4302533.808121/94‑1 (MQ=60)

                                                                                          CCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTG     <  SRR4302533.5009452/94‑1 (MQ=60)

                                                                                            CCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGT   <  SRR4302533.4819253/94‑1 (MQ=60)

                                                                                            CCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGT   >  SRR4302533.2416141/1‑94 (MQ=60)

                                                                                             CACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTA  >  SRR4302533.5069431/1‑94 (MQ=60)

GGATATTCCTGGTAATCAAAGTTAATTTTTTTCTGCTCAAGCTCAGTTTTCAGCCCGGCGATATCCTTGCCGGTTACGACATCTTTAT‑CCCCCACAACCACAGTAAAATTACGTAGTTGCTGGTTGATAGCTGCCGGATCGTTCAGTCGGGCCGCGACACCTTCATCCGGTACGGTTGTCGTGGTA  >  NC_000913/3897106‑3897291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`