Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,917,055 T→C Q144R (CAG→CGG)  atpA ← membrane‑bound ATP synthase, F1 sector, alpha‑subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,917,0550TC99.6% 703.7 / ‑4.7 222Q144R (CAG→CGG) atpAmembrane‑bound ATP synthase, F1 sector, alpha‑subunit
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (103/118);  total (103/119)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.52e-01

Only 100 of 232 total aligned reads displayed.

CCTGTCTGACGGTCACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCTGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTACCGTCGATT  >  NC_000913/3916965‑3917144
                                                                                          |                                                                                         
ccTGTCTGACGGTCACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGa                                                                                         <  2:3277829/93‑1 (MQ=255)
ccTGTCTGACGGTCACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGa                                                                                         <  2:2046775/93‑1 (MQ=255)
ccTGTCTGACGGTCACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGa                                                                                         >  1:6268703/1‑93 (MQ=255)
ccTGTCTGACGGTCACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGa                                                                                         <  1:4701649/93‑1 (MQ=255)
ccTGTCTGACGGTCACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGa                                                                                         >  1:4326331/1‑93 (MQ=255)
 cTGTCTGACGGTCACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGAt                                                                                        >  1:1523234/1‑93 (MQ=255)
  tGTCTGACGGTCACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATc                                                                                       >  2:3816963/1‑93 (MQ=255)
  tGTCTGACGGTCACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATc                                                                                       <  2:154143/93‑1 (MQ=255)
   gTCTGACGGTCACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCt                                                                                      <  2:3239955/93‑1 (MQ=255)
   gTCTGACGGTCACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCt                                                                                      <  2:3024778/93‑1 (MQ=255)
    tCTGACGGTCACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTa                                                                                     <  2:5138327/93‑1 (MQ=255)
    tCTGACGGGCACCGATGATTAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCAGCCGATCTa                                                                                     <  2:4217223/93‑1 (MQ=255)
     cTGACGGTCACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTAc                                                                                    >  2:3356214/1‑93 (MQ=255)
     cTGACGGTCACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTAc                                                                                    >  2:1130554/1‑93 (MQ=255)
      tGACGGTCACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACg                                                                                   <  2:825720/93‑1 (MQ=255)
      tGACGGTCACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACg                                                                                   >  1:745483/1‑93 (MQ=255)
        aCGGTCACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGa                                                                                 <  1:511775/93‑1 (MQ=255)
             cACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGAc                                                                            <  1:2150753/93‑1 (MQ=255)
                cGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGtt                                                                         >  2:4951530/1‑93 (MQ=255)
                cGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGtt                                                                         >  1:3778872/1‑93 (MQ=255)
                 gatgatCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTc                                                                        >  1:3075741/1‑93 (MQ=255)
                 gatgatCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTc                                                                        >  1:1039206/1‑93 (MQ=255)
                   tgatCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGa                                                                      >  1:3008127/1‑93 (MQ=255)
                   tgatCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGa                                                                      >  1:5414537/1‑93 (MQ=255)
                      tCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATaa                                                                   >  1:5176813/1‑93 (MQ=255)
                      tCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATaa                                                                   <  1:2509789/93‑1 (MQ=255)
                      tCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATaa                                                                   <  2:4501261/93‑1 (MQ=255)
                       cAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAAc                                                                  <  2:5732046/93‑1 (MQ=255)
                         aTTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGc                                                                >  1:3783168/1‑93 (MQ=255)
                            cACGCTCACCACGACCGATTGGGAGCATGGAGTCAACGGCTTTATACCCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCg                                                             <  1:6016972/93‑1 (MQ=255)
                             ccGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCgg                                                            <  1:2547340/92‑1 (MQ=255)
                                 tGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCg                                                        >  2:4389841/1‑93 (MQ=255)
                                  gACCACGCCCGATTGGGATCATGGAGTCAGCGCCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGa                                                       <  1:3670192/93‑1 (MQ=255)
                                  gACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGa                                                       >  1:1203911/1‑93 (MQ=255)
                                  gACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGGTCGATAACGCCCGGAGCGa                                                       >  2:3787122/1‑93 (MQ=255)
                                   aCCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGAt                                                      <  2:3161689/93‑1 (MQ=255)
                                      aCGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGc                                                   <  2:363120/93‑1 (MQ=255)
                                       cGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCt                                                  <  1:1701029/93‑1 (MQ=255)
                                        gACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCtt                                                 >  2:4051879/1‑93 (MQ=255)
                                        gACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCtt                                                 >  1:572823/1‑93 (MQ=255)
                                         aCCGATTGGGATCATGGAGTCACCGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCTATTGCTTc                                                <  2:6154381/93‑1 (MQ=255)
                                         aCCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTc                                                <  1:2102118/93‑1 (MQ=255)
                                         aCCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTc                                                >  1:1898693/1‑93 (MQ=255)
                                         aCCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTc                                                <  1:4620568/93‑1 (MQ=255)
                                         aCCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTc                                                >  2:4592296/1‑93 (MQ=255)
                                         aCCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTc                                                <  2:4820540/93‑1 (MQ=255)
                                         aCCGATGGGGAGCATGTAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTc                                                <  2:1279958/93‑1 (MQ=255)
                                          ccGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCt                                               >  2:1266973/1‑93 (MQ=255)
                                          ccGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCt                                               >  2:5606613/1‑93 (MQ=255)
                                            gATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTAc                                             <  1:3180495/93‑1 (MQ=255)
                                            gATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTAc                                             >  1:86542/1‑93 (MQ=255)
                                             aTTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTAca                                            <  2:2769607/93‑1 (MQ=255)
                                              ttGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTAcag                                           >  2:4074120/1‑93 (MQ=255)
                                              ttGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTAcag                                           >  2:2474218/1‑93 (MQ=255)
                                                gggATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTAcagca                                         <  2:4642975/93‑1 (MQ=255)
                                                gggATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTAcagca                                         <  2:2086696/93‑1 (MQ=255)
                                                gggATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTAcagca                                         <  2:2339067/93‑1 (MQ=255)
                                                  gATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTAcagccgg                                       >  2:1802123/1‑90 (MQ=255)
                                                  gATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCaga                                       <  1:4374616/93‑1 (MQ=255)
                                                  gATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCaga                                       <  1:4929376/93‑1 (MQ=255)
                                                    tCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAgaga                                     <  1:5148171/93‑1 (MQ=255)
                                                      aTGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAg                                   >  2:448530/1‑93 (MQ=255)
                                                       tGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGc                                  <  1:397692/93‑1 (MQ=255)
                                                       tGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGc                                  <  1:3906635/93‑1 (MQ=255)
                                                       tGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGc                                  <  1:1867453/93‑1 (MQ=255)
                                                       tGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGc                                  <  1:448301/93‑1 (MQ=255)
                                                        ggATTCACCGGCTTTATACCCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGcc                                 <  2:5408022/93‑1 (MQ=255)
                                                        ggAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGcc                                 <  2:2147608/93‑1 (MQ=255)
                                                        ggAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACCGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGcc                                 >  1:4432162/1‑93 (MQ=255)
                                                         gAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCcg                                >  2:744748/1‑93 (MQ=255)
                                                            tCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCcgtcg                             >  2:3745326/1‑93 (MQ=255)
                                                             cAACGGCTTTATAACCGGTCTGTACTGGACGATCTACGGACTGACGTTCGATAACGACCGGAGCGAGTGCTTCTACAGCAGCGAGGCcgtcgg                            >  2:2801499/1‑92 (MQ=255)
                                                              aaCGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTg                           >  1:1592732/1‑93 (MQ=255)
                                                                 ggCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATc                        <  1:2953868/93‑1 (MQ=255)
                                                                  gCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATcc                       >  1:1489410/1‑93 (MQ=255)
                                                                  gCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATcc                       >  1:940505/1‑93 (MQ=255)
                                                                    tttATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAg                     >  1:269041/1‑93 (MQ=255)
                                                                     ttATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGc                    >  2:5530828/1‑93 (MQ=255)
                                                                     ttATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGc                    <  1:410131/93‑1 (MQ=255)
                                                                     taataCCCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGc                    <  1:6187692/91‑1 (MQ=255)
                                                                       ataACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCgg                  >  2:1445747/1‑93 (MQ=255)
                                                                        taACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGa                 >  2:6257692/1‑93 (MQ=255)
                                                                         aaCCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGAc                >  2:5985657/1‑93 (MQ=255)
                                                                           ccGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCt              <  2:1055952/93‑1 (MQ=255)
                                                                            cGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCtt             <  2:6038764/93‑1 (MQ=255)
                                                                             ggTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCttt            >  1:3350652/1‑93 (MQ=255)
                                                                              gTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACtttcc           >  1:1072148/1‑91 (MQ=255)
                                                                              gTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTa           >  2:1954280/1‑93 (MQ=255)
                                                                              gTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTa           >  1:3558419/1‑93 (MQ=255)
                                                                              gTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTa           >  1:5456248/1‑93 (MQ=255)
                                                                              gTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTa           <  2:2571408/93‑1 (MQ=255)
                                                                              gTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTa           <  1:1016139/93‑1 (MQ=255)
                                                                                cTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTAcc         <  2:4724839/93‑1 (MQ=255)
                                                                                  gTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTACCGt       <  1:3874053/93‑1 (MQ=255)
                                                                                    cccGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTACCGTCg     <  2:5863564/92‑1 (MQ=255)
                                                                                    aCCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTACCGTCg     >  2:4074985/1‑93 (MQ=255)
                                                                                     ccGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTACCGTCGa    <  1:5975104/93‑1 (MQ=255)
                                                                                      cGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTACCGTCGAt   <  1:2299317/93‑1 (MQ=255)
                                                                                       ggCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTACCGTCGAtt  <  2:569043/93‑1 (MQ=255)
                                                                                       ggCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTACCGTCGAtt  <  2:3503428/93‑1 (MQ=255)
                                                                                          |                                                                                         
CCTGTCTGACGGTCACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCTGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTACCGTCGATT  >  NC_000913/3916965‑3917144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTACCTGTCTGACGGTCACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCTGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTACCGTCGATTGGTG  >  NC_000913/3916962‑3917148
                                                                                             |                                                                                             
TTACCTGTCTGACGGTCACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCC                                                                                               <  SRR4302533.6187713/94‑1 (MQ=60)
TTACCTGTCTGACGGTCACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCC                                                                                               >  SRR4302533.1402767/1‑94 (MQ=60)
 TACCTGTCTGACGGTCACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCG                                                                                              <  SRR4302533.4488825/94‑1 (MQ=60)
 TACCTGTCTGACGGCCACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCG                                                                                              >  SRR4302533.2190515/1‑94 (MQ=60)
  ACCTGTCTGACGGTCACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGA                                                                                             >  SRR4302533.6306161/1‑94 (MQ=60)
  ACCTGTCTGACGGTCACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGA                                                                                             >  SRR4302533.4352393/1‑94 (MQ=60)
   CCTGTCTGACGGTCACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGAT                                                                                            <  SRR4302533.4730154/94‑1 (MQ=60)
   CCTGTCTGACGGTCACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGAT                                                                                            >  SRR4302533.1532401/1‑94 (MQ=60)
    CTGTCTGACGGTCACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATC                                                                                           <  SRR4302533.273200/94‑1 (MQ=60)
     TGTCTGACGGTCACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCT                                                                                          >  SRR4302533.2211845/1‑94 (MQ=60)
     TGTCTGACGGTCACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCT                                                                                          <  SRR4302533.3860955/94‑1 (MQ=60)
      GTCTGACGGTCACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTA                                                                                         >  SRR4302533.759357/1‑94 (MQ=60)
       TCTGACGGTCGCCGATGATCAATTCCCGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTAC                                                                                        <  SRR4302533.3913931/94‑1 (MQ=60)
        CTGACGGTCACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACG                                                                                       >  SRR4302533.4502816/1‑94 (MQ=60)
        CTGACGGTCACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACG                                                                                       >  SRR4302533.749869/1‑94 (MQ=60)
         TGACGGTCACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGG                                                                                      <  SRR4302533.1544651/94‑1 (MQ=60)
           ACGGTCACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGAC                                                                                    <  SRR4302533.514815/94‑1 (MQ=60)
           ACGGTCACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGAC                                                                                    <  SRR4302533.1058822/94‑1 (MQ=60)
            CGGTCACCGATGATCAATTCACGCTGCCCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACT                                                                                   <  SRR4302533.2125422/94‑1 (MQ=60)
            CGGTCACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACT                                                                                   >  SRR4302533.5172237/1‑94 (MQ=60)
               TCACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGAC                                                                                <  SRR4302533.1264763/94‑1 (MQ=60)
                CACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACG                                                                               <  SRR4302533.5498719/94‑1 (MQ=60)
                CACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACG                                                                               <  SRR4302533.2163997/94‑1 (MQ=60)
                  CCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTT                                                                             <  SRR4302533.4495648/94‑1 (MQ=60)
                  CCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTT                                                                             >  SRR4302533.3802024/1‑94 (MQ=60)
                   CGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTC                                                                            >  SRR4302533.981170/1‑94 (MQ=60)
                   CGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTC                                                                            >  SRR4302533.3094618/1‑94 (MQ=60)
                   CGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTC                                                                            >  SRR4302533.3023637/1‑94 (MQ=60)
                   CGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTC                                                                            >  SRR4302533.1045315/1‑94 (MQ=60)
                     ATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGA                                                                          >  SRR4302533.5447096/1‑94 (MQ=60)
                     ATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGA                                                                          >  SRR4302533.10252/1‑94 (MQ=60)
                     ATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGA                                                                          >  SRR4302533.3026530/1‑94 (MQ=60)
                      TGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGAT                                                                         <  SRR4302533.5055231/94‑1 (MQ=60)
                       GATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATA                                                                        >  SRR4302533.4760602/1‑94 (MQ=60)
                       GATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATA                                                                        <  SRR4302533.3144671/94‑1 (MQ=60)
                        ATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAA                                                                       >  SRR4302533.5208078/1‑94 (MQ=60)
                         TCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAAC                                                                      >  SRR4302533.3586342/1‑94 (MQ=60)
                         TCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAAC                                                                      <  SRR4302533.2525252/94‑1 (MQ=60)
                         TCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAAC                                                                      <  SRR4302533.6248143/94‑1 (MQ=60)
                           AATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGC                                                                    >  SRR4302533.3806335/1‑94 (MQ=60)
                             ATCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCC                                                                  <  SRR4302533.1883925/94‑1 (MQ=60)
                              TCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCG                                                                 <  SRR4302533.1910770/94‑1 (MQ=60)
                               CACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGG                                                                >  SRR4302533.896590/1‑94 (MQ=60)
                               CACGCTCACCACGACCGATTGGGAGCATGGAGTCAACGGCTTTATACCCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGG                                                                <  SRR4302533.6053429/94‑1 (MQ=60)
                                CCGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGA                                                               <  SRR4302533.2563018/94‑1 (MQ=60)
                                ACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGA                                                               >  SRR4302533.1522892/1‑94 (MQ=60)
                                 CGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAG                                                              >  SRR4302533.2438080/1‑94 (MQ=60)
                                  CCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGC                                                             <  SRR4302533.3137462/94‑1 (MQ=60)
                                   CTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCG                                                            <  SRR4302533.6024058/94‑1 (MQ=60)
                                   CTGACAACGCCCGATGGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACTGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCG                                                            <  SRR4302533.2528559/94‑1 (MQ=60)
                                    TGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGA                                                           >  SRR4302533.1211146/1‑94 (MQ=60)
                                    TGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGA                                                           >  SRR4302533.3951387/1‑94 (MQ=60)
                                     GACCACGCCCGATTGGGATCATGGAGTCAGCGCCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGAT                                                          <  SRR4302533.3692752/94‑1 (MQ=60)
                                     GACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGAT                                                          <  SRR4302533.3656228/94‑1 (MQ=60)
                                       CCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTG                                                        <  SRR4302533.5211423/94‑1 (MQ=60)
                                        CACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGC                                                       <  SRR4302533.479239/94‑1 (MQ=60)
                                          CGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTT                                                     <  SRR4302533.1711338/94‑1 (MQ=60)
                                          CGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTT                                                     >  SRR4302533.576203/1‑94 (MQ=60)
                                           GACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTC                                                    >  SRR4302533.1910285/1‑94 (MQ=60)
                                            ACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCT                                                   <  SRR4302533.4648572/94‑1 (MQ=60)
                                            ACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCT                                                   <  SRR4302533.2115080/94‑1 (MQ=60)
                                             CCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTA                                                  <  SRR4302533.1202269/94‑1 (MQ=60)
                                              CGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTAC                                                 >  SRR4302533.87097/1‑94 (MQ=60)
                                              CGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTAC                                                 <  SRR4302533.868117/94‑1 (MQ=60)
                                              CGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTAC                                                 >  SRR4302533.2709921/1‑94 (MQ=60)
                                               GATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACA                                                <  SRR4302533.3200060/94‑1 (MQ=60)
                                               GATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACA                                                >  SRR4302533.1693822/1‑94 (MQ=60)
                                                ATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAG                                               <  SRR4302533.18326/94‑1 (MQ=60)
                                                ATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAG                                               <  SRR4302533.3803312/94‑1 (MQ=60)
                                                    GGATCATGGAGTCAACGGCATTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGA                                           >  SRR4302533.1713974/1‑94 (MQ=60)
                                                     GATCATGGATTAAACGCCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAG                                          <  SRR4302533.383567/94‑1 (MQ=60)
                                                     GATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAG                                          <  SRR4302533.4959227/94‑1 (MQ=60)
                                                     GATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAG                                          <  SRR4302533.1394135/94‑1 (MQ=60)
                                                     GATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAG                                          <  SRR4302533.4401022/94‑1 (MQ=60)
                                                       TCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAA                                        <  SRR4302533.5179276/94‑1 (MQ=60)
                                                        CATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCAACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAG                                       >  SRR4302533.650972/1‑94 (MQ=60)
                                                         ATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGC                                      <  SRR4302533.4398573/94‑1 (MQ=60)
                                                         ATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGC                                      <  SRR4302533.4178486/94‑1 (MQ=60)
                                                          TGGAGTCAACGTCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCC                                     <  SRR4302533.3028807/94‑1 (MQ=60)
                                                          TGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCC                                     <  SRR4302533.450982/94‑1 (MQ=60)
                                                          TGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCC                                     <  SRR4302533.1878838/94‑1 (MQ=60)
                                                          TGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCC                                     <  SRR4302533.400097/94‑1 (MQ=60)
                                                          TGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCC                                     <  SRR4302533.3930498/94‑1 (MQ=60)
                                                          TGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACCGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCC                                     >  SRR4302533.4458955/1‑94 (MQ=60)
                                                           GGAGTCAAGGGCTTTATACCCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCG                                    <  SRR4302533.3544090/94‑1 (MQ=60)
                                                           GGAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCG                                    >  SRR4302533.5921502/1‑94 (MQ=60)
                                                            GAGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGT                                   >  SRR4302533.3759264/1‑94 (MQ=60)
                                                             AGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTC                                  >  SRR4302533.3265895/1‑94 (MQ=60)
                                                             AGTCAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTC                                  <  SRR4302533.4083563/94‑1 (MQ=60)
                                                                CAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTG                               >  SRR4302533.2080533/1‑94 (MQ=60)
                                                                CAACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTG                               >  SRR4302533.1602370/1‑94 (MQ=60)
                                                                 AACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGA                              >  SRR4302533.5656388/1‑94 (MQ=60)
                                                                  ACGGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGAT                             >  SRR4302533.5199787/1‑94 (MQ=60)
                                                                    GGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTTACGTTCGATAACGCCCGGAGCGATTGCTTCTACCGCAGAGAAGCCGGCGTGATAC                           >  SRR4302533.5085548/1‑94 (MQ=60)
                                                                    GGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCC                           >  SRR4302533.1498394/1‑94 (MQ=60)
                                                                    GGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCC                           >  SRR4302533.946029/1‑94 (MQ=60)
                                                                    GGCTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCC                           <  SRR4302533.2971933/94‑1 (MQ=60)
                                                                      CTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAG                         >  SRR4302533.4697338/1‑94 (MQ=60)
                                                                      CTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAG                         >  SRR4302533.2511446/1‑94 (MQ=60)
                                                                      CTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAG                         <  SRR4302533.4447207/94‑1 (MQ=60)
                                                                      CTTTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAG                         >  SRR4302533.270674/1‑94 (MQ=60)
                                                                        TTATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCG                       <  SRR4302533.412590/94‑1 (MQ=60)
                                                                        TAATACCCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCG                       <  SRR4302533.6224831/94‑1 (MQ=60)
                                                                          ATAACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGA                     >  SRR4302533.5454908/1‑94 (MQ=60)
                                                                             ACCGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCT                  >  SRR4302533.4542330/1‑94 (MQ=60)
                                                                               CGGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTT                >  SRR4302533.3371350/1‑94 (MQ=60)
                                                                                GGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACtttcc               >  SRR4302533.1078466/1‑89 (MQ=60)
                                                                                GGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTA               >  SRR4302533.5489119/1‑94 (MQ=60)
                                                                                GGTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTA               >  SRR4302533.3580347/1‑94 (MQ=60)
                                                                                 GTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTAC              <  SRR4302533.2572555/94‑1 (MQ=60)
                                                                                 GTCTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTAC              <  SRR4302533.1022112/94‑1 (MQ=60)
                                                                                   CTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTACCG            <  SRR4302533.614254/94‑1 (MQ=60)
                                                                                   CTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTACCG            <  SRR4302533.6163734/94‑1 (MQ=60)
                                                                                   CTGTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTACCG            >  SRR4302533.4148047/1‑94 (MQ=60)
                                                                                     GTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTACCGTC          <  SRR4302533.3897703/94‑1 (MQ=60)
                                                                                     GTACCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTACCGTC          >  SRR4302533.1684763/1‑94 (MQ=60)
                                                                                        CCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGCGAAGCCGTCGTGATCCAGCGGACCTTTACCGTCGAT       >  SRR4302533.518616/1‑94 (MQ=60)
                                                                                        CCGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTACCGTCGAT       <  SRR4302533.6011273/94‑1 (MQ=60)
                                                                                         CGGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTACCGTCGATT      <  SRR4302533.2313572/94‑1 (MQ=60)
                                                                                          GGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTACCGTCGATTG     >  SRR4302533.4095724/1‑94 (MQ=60)
                                                                                          GGCCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTACCGTCGATTG     >  SRR4302533.4779722/1‑94 (MQ=60)
                                                                                            CCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTACCGTCGATTGGT   >  SRR4302533.5786946/1‑94 (MQ=60)
                                                                                            CCGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTACCGTCGATTGGC   >  SRR4302533.6086017/1‑94 (MQ=60)
                                                                                             CGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTACCGTCGATTGGTG  <  SRR4302533.3732841/94‑1 (MQ=60)
                                                                                             CGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTACCGTCGATTGGTG  >  SRR4302533.569412/1‑94 (MQ=60)
                                                                                             CGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTACCGTCGATTGGTG  <  SRR4302533.4417756/94‑1 (MQ=60)
                                                                                             |                                                                                             
TTACCTGTCTGACGGTCACCGATGATCAATTCACGCTGACCACGACCGATTGGGATCATGGAGTCAACGGCTTTATAACCGGTCTGTACCGGCTGATCTACGGACTGACGTTCGATAACGCCCGGAGCGATTGCTTCTACAGCAGAGAAGCCGTCGTGATCCAGCGGACCTTTACCGTCGATTGGTG  >  NC_000913/3916962‑3917148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑