Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,930,677	+G	intergenic (+175/‑264)	kup → / → rbsD	low affinity potassium transport system/RbsD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,930,676	1	.	G	99.6%	722.3 / ‑3.9	224	intergenic (+174/‑265)	kup/rbsD	low affinity potassium transport system/RbsD
Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (127/96); total (128/96)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.93e-01

Only 100 of 242 total aligned reads displayed.

GTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TC‑GCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAA  >  NC_000913/3930584‑3930762

gTAGAAGAAGTTTTCCGCCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑Tc                                                                                         <  1:550397/93‑1 (MQ=255)

    aagaagTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TC‑ggca                                                                                    >  2:1652781/1‑90 (MQ=255)

         gTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGc                                                                                >  2:2107967/1‑93 (MQ=255)

          ttttCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGcc                                                                               >  2:5338168/1‑93 (MQ=255)

           tttCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGccc                                                                              <  1:5314598/93‑1 (MQ=255)

           tttCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGccc                                                                              >  2:5779169/1‑93 (MQ=255)

              ccACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTg                                                                           <  2:4038271/93‑1 (MQ=255)

              ccACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTg                                                                           >  2:2035381/1‑93 (MQ=255)

               cgccGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTgg                                                                          >  2:3973705/3‑93 (MQ=255)

                aCCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGt                                                                         >  2:4140187/1‑93 (MQ=255)

                 ccGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTa                                                                        >  1:2958999/1‑93 (MQ=255)

                  cGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTAt                                                                       <  1:5934631/93‑1 (MQ=255)

                  cGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTAt                                                                       >  1:385917/1‑93 (MQ=255)

                   gCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTAtt                                                                      >  1:5886110/1‑93 (MQ=255)

                   gCTGCGGTCTGGAAAGATTAAGTTGCCGGATGATGTAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTAtt                                                                      <  2:3445188/93‑1 (MQ=255)

                    cTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTAttt                                                                     >  1:4927435/1‑93 (MQ=255)

                    cTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTAttt                                                                     <  1:1797601/93‑1 (MQ=255)

                      gCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGc                                                                   >  2:156182/1‑93 (MQ=255)

                        ggTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGt                                                                 <  1:3567387/93‑1 (MQ=255)

                         gTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTTG‑GCAAACGCCCGTGGTATTTGCGtt                                                                >  2:1100918/1‑92 (MQ=255)

                            tgaaaGAATTAAGTTGACGAATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTgc                                                             <  1:3430615/90‑1 (MQ=255)

                             ggAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTgcg                                                            >  2:5556040/1‑93 (MQ=255)

                              gAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCGAACGCCCGTGGTATTTGCGTCTgcgc                                                           >  1:764834/1‑93 (MQ=255)

                              gAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTgcgc                                                           >  2:3785720/1‑93 (MQ=255)

                              gAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTgcgc                                                           >  1:1036083/1‑93 (MQ=255)

                              gAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTgcgc                                                           >  1:3888729/1‑93 (MQ=255)

                                aGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCgg                                                         >  1:1134960/1‑93 (MQ=255)

                                 ggATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGc                                                        <  1:1365604/93‑1 (MQ=255)

                                  gATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCa                                                       >  1:5538411/1‑93 (MQ=255)

                                   aTTAAGTTGCCTGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCaa                                                      >  1:5983349/1‑93 (MQ=255)

                                    ttAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAg                                                     >  2:1877417/1‑93 (MQ=255)

                                    ttAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAg                                                     <  1:6161428/93‑1 (MQ=255)

                                     tAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGc                                                    <  2:1178274/93‑1 (MQ=255)

                                        gTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGt                                                 >  1:2049844/1‑93 (MQ=255)

                                          tGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTAc                                               <  1:3714032/93‑1 (MQ=255)

                                           gCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACt                                              >  2:787644/1‑93 (MQ=255)

                                           gCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACt                                              >  1:4736719/1‑93 (MQ=255)

                                           gCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACt                                              >  1:1276475/1‑93 (MQ=255)

                                            ccGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACtt                                             <  1:212578/93‑1 (MQ=255)

                                              ggATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTtgc                                           >  2:3019828/1‑93 (MQ=255)

                                              ggATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTtgc                                           >  2:2727837/1‑93 (MQ=255)

                                              ggATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTtgc                                           >  2:1871101/1‑93 (MQ=255)

                                               gatgatGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTtgct                                          >  2:6257622/1‑93 (MQ=255)

                                                 tgatgGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTtgctgc                                        >  1:4338312/1‑93 (MQ=255)

                                                   atgGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTtgctgctg                                      >  1:1166707/1‑93 (MQ=255)

                                                   atgGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTtgctgctg                                      >  2:2200884/1‑93 (MQ=255)

                                                    tgGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTtgctgctgc                                     <  1:5383235/93‑1 (MQ=255)

                                                    tgGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTtgctgctgc                                     <  2:3473500/93‑1 (MQ=255)

                                                    aggAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTtgctgctgc                                     <  1:3356262/92‑1 (MQ=255)

                                                     ggAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCa                                    >  1:4830444/1‑93 (MQ=255)

                                                      gAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCaa                                   <  1:5121188/93‑1 (MQ=255)

                                                       aaaCCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAAc                                  <  1:4640915/93‑1 (MQ=255)

                                                       aaaCCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAAc                                  >  1:743264/1‑93 (MQ=255)

                                                        aaCCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACg                                 >  1:6241594/1‑93 (MQ=255)

                                                        aaCCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACg                                 >  2:6015386/1‑93 (MQ=255)

                                                         aCCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGt                                >  2:4264163/1‑93 (MQ=255)

                                                         aCCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGt                                >  2:1029023/1‑93 (MQ=255)

                                                         aCCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGt                                >  1:1398309/1‑93 (MQ=255)

                                                          cctcctTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTa                               <  2:4294862/93‑1 (MQ=255)

                                                          cctcctTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTa                               >  2:4505564/1‑93 (MQ=255)

                                                            tcctTCTTTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAAt                             <  2:5932107/93‑1 (MQ=255)

                                                              cttcttTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATgc                           <  2:49196/93‑1 (MQ=255)

                                                               ttcttTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATgcg                          <  2:1446410/93‑1 (MQ=255)

                                                               ttcttTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATgcg                          <  2:6241604/93‑1 (MQ=255)

                                                                tcttTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATgcgc                         >  1:2792228/1‑93 (MQ=255)

                                                                tcttTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATgcgc                         <  2:2916786/93‑1 (MQ=255)

                                                                tcttTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATgcgc                         >  1:1315979/1‑93 (MQ=255)

                                                                tcttTATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATgcgc                         <  1:3499724/93‑1 (MQ=255)

                                                                  tttATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTg                       >  2:4523584/1‑93 (MQ=255)

                                                                  tttATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTg                       <  1:863314/93‑1 (MQ=255)

                                                                   ttATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGc                      <  1:3274637/93‑1 (MQ=255)

                                                                    tATGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAatgcgatgcg                     >  2:2064127/1‑93 (MQ=255)

                                                                     aTGTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGt                    >  2:5255566/1‑93 (MQ=255)

                                                                     aTGTCGCAAGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGt                    >  2:6144986/1‑93 (MQ=255)

                                                                       gTCGCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGc                  >  1:935581/1‑93 (MQ=255)

                                                                          gCATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCAcc               >  2:3532078/1‑93 (MQ=255)

                                                                           cATGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCa              <  2:2890619/93‑1 (MQ=255)

                                                                            aTGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAg             >  2:3269937/1‑93 (MQ=255)

                                                                            aTGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAg             <  2:3801681/93‑1 (MQ=255)

                                                                             tGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGa            <  1:1763434/93‑1 (MQ=255)

                                                                             tGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGa            >  1:1599047/1‑93 (MQ=255)

                                                                             tGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGa            >  2:4715467/1‑93 (MQ=255)

                                                                             tGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGa            <  2:278361/93‑1 (MQ=255)

                                                                             tGAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGa            >  1:4083778/1‑93 (MQ=255)

                                                                              gAGTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGAt           <  1:5710198/93‑1 (MQ=255)

                                                                                gTCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCa         >  1:3653087/1‑93 (MQ=255)

                                                                                 tCGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCaa        <  2:2366098/93‑1 (MQ=255)

                                                                                  cGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAAt       <  1:2921725/93‑1 (MQ=255)

                                                                                  cGTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAAt       >  2:3106345/1‑93 (MQ=255)

                                                                                   gTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGGAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAAtt      >  1:4442365/1‑93 (MQ=255)

                                                                                   gTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAAtt      <  1:4016502/93‑1 (MQ=255)

                                                                                   gTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAAtt      <  2:3601513/93‑1 (MQ=255)

                                                                                   gTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAAtt      >  2:2253811/1‑93 (MQ=255)

                                                                                   gTTGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAAtt      <  2:5704304/93‑1 (MQ=255)

                                                                                    ttGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAAttt     <  1:6059723/93‑1 (MQ=255)

                                                                                    ttGATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAAttt     >  1:2540797/1‑93 (MQ=255)

                                                                                      gATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGa   >  1:4598858/1‑93 (MQ=255)

                                                                                      gATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGa   >  2:691037/1‑93 (MQ=255)

                                                                                      gATCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGa   <  1:2223933/93‑1 (MQ=255)

                                                                                       aTCC‑TCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGaa  >  1:3401840/1‑93 (MQ=255)

GTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCC‑TC‑GCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAA  >  NC_000913/3930584‑3930762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTC‑GCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCC  >  NC_000913/3930584‑3930770

GTAGAAGAAGTTTTCCGCCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTC‑G                                                                                               <  SRR4302533.553662/94‑1 (MQ=60)

GTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAGCCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTC‑G                                                                                               <  SRR4302533.1385486/94‑1 (MQ=60)

GTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTC‑G                                                                                               <  SRR4302533.3878968/94‑1 (MQ=60)

  AGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTC‑Ggc                                                                                             >  SRR4302533.5688931/1‑92 (MQ=60)

   GAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTC‑Ggca                                                                                            >  SRR4302533.5538833/1‑91 (MQ=60)

    AAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAA                                                                                            >  SRR4302533.2116422/1‑94 (MQ=60)

    AAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAA                                                                                            >  SRR4302533.3959854/1‑94 (MQ=60)

     AGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA                                                                                           >  SRR4302533.1192550/1‑94 (MQ=60)

      GAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAAC                                                                                          <  SRR4302533.598900/94‑1 (MQ=60)

      GAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAAC                                                                                          <  SRR4302533.2823777/94‑1 (MQ=60)

      GAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAAC                                                                                          <  SRR4302533.2000515/94‑1 (MQ=60)

       AAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACG                                                                                         <  SRR4302533.1365614/94‑1 (MQ=60)

       AAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACG                                                                                         >  SRR4302533.3629117/1‑94 (MQ=60)

         GTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCC                                                                                       >  SRR4302533.3451391/1‑94 (MQ=60)

          TTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCC                                                                                      <  SRR4302533.4238455/94‑1 (MQ=60)

           TTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCG                                                                                     <  SRR4302533.5346446/94‑1 (MQ=60)

              CCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGG                                                                                  >  SRR4302533.1801099/1‑94 (MQ=60)

               CACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGT                                                                                 >  SRR4302533.2194979/1‑94 (MQ=60)

               CACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGT                                                                                 >  SRR4302533.5943064/1‑94 (MQ=60)

               CACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGT                                                                                 <  SRR4302533.1683335/94‑1 (MQ=60)

                ACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTA                                                                                >  SRR4302533.2977084/1‑94 (MQ=60)

                ACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTA                                                                                >  SRR4302533.2826245/1‑94 (MQ=60)

                 CCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTAT                                                                               >  SRR4302533.4659452/1‑94 (MQ=60)

                 CCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTAT                                                                               >  SRR4302533.388247/1‑94 (MQ=60)

                  CGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATT                                                                              >  SRR4302533.1248918/1‑94 (MQ=60)

                  CGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATT                                                                              >  SRR4302533.3556776/1‑94 (MQ=60)

                  CGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATT                                                                              >  SRR4302533.2833503/1‑94 (MQ=60)

                  CGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATT                                                                              <  SRR4302533.4788033/94‑1 (MQ=60)

                  CGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATT                                                                              >  SRR4302533.5921675/1‑94 (MQ=60)

                  CGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATT                                                                              <  SRR4302533.5970508/94‑1 (MQ=60)

                   GCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTT                                                                             >  SRR4302533.4957276/1‑94 (MQ=60)

                   GCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCAGCAAACGCTCGTGGTATTT                                                                             >  SRR4302533.3983821/1‑94 (MQ=60)

                    CTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTG                                                                            >  SRR4302533.4980323/1‑94 (MQ=60)

                    CTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTG                                                                            <  SRR4302533.1940095/94‑1 (MQ=60)

                    CTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTG                                                                            <  SRR4302533.1808539/94‑1 (MQ=60)

                      GCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCGCGTGGTATTTGCG                                                                          <  SRR4302533.3427503/94‑1 (MQ=60)

                        GGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTC                                                                        <  SRR4302533.3589371/94‑1 (MQ=60)

                        GGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTC                                                                        <  SRR4302533.3589370/94‑1 (MQ=60)

                         GTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCT                                                                       >  SRR4302533.5012065/1‑94 (MQ=60)

                         GTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCT                                                                       >  SRR4302533.1289771/1‑94 (MQ=60)

                          TCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTG                                                                      <  SRR4302533.6133133/94‑1 (MQ=60)

                           CTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGC                                                                     <  SRR4302533.3460109/94‑1 (MQ=60)

                           CTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGACCGTGGTATTTGCGTCTGC                                                                     >  SRR4302533.854374/1‑94 (MQ=60)

                            TGAAAGAATTAAGTTGACGAATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCG                                                                    <  SRR4302533.3451808/94‑1 (MQ=60)

                             GGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCGAACGCCCGTGGTATTTGCGTCTGCGC                                                                   >  SRR4302533.769338/1‑94 (MQ=60)

                             GGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGC                                                                   >  SRR4302533.3912454/1‑94 (MQ=60)

                             GGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGC                                                                   >  SRR4302533.1042174/1‑94 (MQ=60)

                              GAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCG                                                                  <  SRR4302533.2969396/94‑1 (MQ=60)

                               AAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGG                                                                 >  SRR4302533.1141709/1‑94 (MQ=60)

                                AGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGC                                                                >  SRR4302533.4091567/1‑94 (MQ=60)

                                 GGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCA                                                               >  SRR4302533.4593232/1‑94 (MQ=60)

                                 GGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCA                                                               <  SRR4302533.1373815/94‑1 (MQ=60)

                                 GGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCA                                                               >  SRR4302533.5571857/1‑94 (MQ=60)

                                  GATTAAGTTGCCTGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAA                                                              >  SRR4302533.6019585/1‑94 (MQ=60)

                                    TTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGC                                                            <  SRR4302533.6198480/94‑1 (MQ=60)

                                     TAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCT                                                           <  SRR4302533.5925725/94‑1 (MQ=60)

                                       AGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGT                                                         >  SRR4302533.2062490/1‑94 (MQ=60)

                                          TGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACT                                                      >  SRR4302533.1284147/1‑94 (MQ=60)

                                          TGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACT                                                      <  SRR4302533.3736836/94‑1 (MQ=60)

                                          TGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACT                                                      >  SRR4302533.4765441/1‑94 (MQ=60)

                                           GCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTT                                                     <  SRR4302533.628101/94‑1 (MQ=60)

                                           GCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTT                                                     <  SRR4302533.3211954/94‑1 (MQ=60)

                                            CCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTG                                                    <  SRR4302533.213915/94‑1 (MQ=60)

                                                ATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGC                                                >  SRR4302533.4364460/1‑94 (MQ=60)

                                                ATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGC                                                >  SRR4302533.765653/1‑94 (MQ=60)

                                                  GATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTG                                              >  SRR4302533.1173706/1‑94 (MQ=60)

                                                  GATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTG                                              >  SRR4302533.1172919/1‑94 (MQ=60)

                                                   ATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGC                                             >  SRR4302533.4515165/1‑94 (MQ=60)

                                                   ATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGC                                             >  SRR4302533.320746/1‑94 (MQ=60)

                                                    TGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCA                                            >  SRR4302533.4859721/1‑94 (MQ=60)

                                                    TGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCA                                            <  SRR4302533.5415579/94‑1 (MQ=60)

                                                    AGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCA                                            <  SRR4302533.3376998/94‑1 (MQ=60)

                                                      GAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAAC                                          <  SRR4302533.5152129/94‑1 (MQ=60)

                                                      GAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAAC                                          >  SRR4302533.747640/1‑94 (MQ=60)

                                                      GAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAAC                                          <  SRR4302533.1272397/94‑1 (MQ=60)

                                                       AAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACG                                         >  SRR4302533.6278963/1‑94 (MQ=60)

                                                       AAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACG                                         <  SRR4302533.4669033/94‑1 (MQ=60)

                                                       AAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGAGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACG                                         <  SRR4302533.3400304/94‑1 (MQ=60)

                                                        AACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGT                                        >  SRR4302533.1406736/1‑94 (MQ=60)

                                                        AACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGT                                        >  SRR4302533.875967/1‑94 (MQ=60)

                                                         ACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCCAGCTGTACTTGCTGCTGCACCGTA                                       >  SRR4302533.6239100/1‑94 (MQ=60)

                                                          CCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAA                                      >  SRR4302533.3891221/1‑94 (MQ=60)

                                                          CCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAA                                      >  SRR4302533.4843640/1‑94 (MQ=60)

                                                           CTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAAT                                     <  SRR4302533.5901316/94‑1 (MQ=60)

                                                           CTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAAT                                     <  SRR4302533.5360387/94‑1 (MQ=60)

                                                           CTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAAT                                     <  SRR4302533.5478149/94‑1 (MQ=60)

                                                            TCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATG                                    <  SRR4302533.5274482/94‑1 (MQ=60)

                                                            TCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATG                                    <  SRR4302533.6311195/94‑1 (MQ=60)

                                                            TCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATG                                    <  SRR4302533.3705353/94‑1 (MQ=60)

                                                            CCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATG                                    <  SRR4302533.36958/94‑1 (MQ=60)

                                                             CCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGC                                   >  SRR4302533.2733785/1‑94 (MQ=60)

                                                              CTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCG                                  <  SRR4302533.5419170/94‑1 (MQ=60)

                                                              CTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCG                                  >  SRR4302533.6120341/1‑94 (MQ=60)

                                                               TTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGC                                 >  SRR4302533.1323873/1‑94 (MQ=60)

                                                               TTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGC                                 >  SRR4302533.2809402/1‑94 (MQ=60)

                                                                TCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCT                                <  SRR4302533.3521307/94‑1 (MQ=60)

                                                                 CTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTG                               <  SRR4302533.494952/94‑1 (MQ=60)

                                                                 CTTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTG                               <  SRR4302533.3376690/94‑1 (MQ=60)

                                                                  TTTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGC                              <  SRR4302533.868372/94‑1 (MQ=60)

                                                                   TTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCG                             <  SRR4302533.3294854/94‑1 (MQ=60)

                                                                   TTATGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGATGCG                             >  SRR4302533.11093/1‑94 (MQ=60)

                                                                   TTATGACGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCG                             >  SRR4302533.6016478/1‑94 (MQ=60)

                                                                      TGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGC                          >  SRR4302533.941073/1‑94 (MQ=60)

                                                                      TGTCGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGC                          <  SRR4302533.2249960/94‑1 (MQ=60)

                                                                         CGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACC                       >  SRR4302533.4734563/1‑94 (MQ=60)

                                                                         CGCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACC                       >  SRR4302533.1435241/1‑94 (MQ=60)

                                                                          GCATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCA                      >  SRR4302533.5585069/1‑94 (MQ=60)

                                                                           CATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAG                     <  SRR4302533.4421426/94‑1 (MQ=60)

                                                                            ATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGA                    <  SRR4302533.6096329/94‑1 (MQ=60)

                                                                            ATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGA                    >  SRR4302533.1608728/1‑94 (MQ=60)

                                                                            ATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGA                    >  SRR4302533.4108636/1‑94 (MQ=60)

                                                                            ATGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGACAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGA                    >  SRR4302533.1677518/1‑94 (MQ=60)

                                                                             TGAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGAT                   <  SRR4302533.1774172/94‑1 (MQ=60)

                                                                              GAGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATC                  <  SRR4302533.5744687/94‑1 (MQ=60)

                                                                               AGTCGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCA                 >  SRR4302533.3675550/1‑94 (MQ=60)

                                                                                  CGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGGAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATT              >  SRR4302533.4469224/1‑94 (MQ=60)

                                                                                  CGTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATT              <  SRR4302533.2939618/94‑1 (MQ=60)

                                                                                   GTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTT             >  SRR4302533.2556438/1‑94 (MQ=60)

                                                                                   GTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTT             >  SRR4302533.785231/1‑94 (MQ=60)

                                                                                   GTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTT             >  SRR4302533.2196205/1‑94 (MQ=60)

                                                                                   GTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTT             >  SRR4302533.951784/1‑94 (MQ=60)

                                                                                   GTTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTT             <  SRR4302533.4040956/94‑1 (MQ=60)

                                                                                    TTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTG            <  SRR4302533.6096405/94‑1 (MQ=60)

                                                                                    TTGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTG            >  SRR4302533.1833065/1‑94 (MQ=60)

                                                                                     TGATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGA           >  SRR4302533.4626720/1‑94 (MQ=60)

                                                                                      GATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAA          >  SRR4302533.3422846/1‑94 (MQ=60)

                                                                                      GATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAA          <  SRR4302533.2237667/94‑1 (MQ=60)

                                                                                       ATCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAA         <  SRR4302533.133781/94‑1 (MQ=60)

                                                                                        TCCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAAT        >  SRR4302533.5980066/1‑94 (MQ=60)

                                                                                         CCTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATC       <  SRR4302533.4127728/94‑1 (MQ=60)

                                                                                          CTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCC      >  SRR4302533.5124364/1‑94 (MQ=60)

                                                                                          CTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCC      >  SRR4302533.3090086/1‑94 (MQ=60)

                                                                                          CTCGGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGATGCTGCAACGTCATGCGGTGAGTGTAACAGATAAATTTGAAATCA      >  SRR4302533.3090087/1‑94 (MQ=60)

                                                                                            cgGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCG    <  SRR4302533.4556460/92‑1 (MQ=60)

                                                                                            cgGCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCG    >  SRR4302533.5302454/3‑94 (MQ=60)

                                                                                            G‑GCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGC   <  SRR4302533.3329734/94‑1 (MQ=60)

                                                                                            G‑GCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGC   >  SRR4302533.389019/1‑94 (MQ=60)

                                                                                              GCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCC  <  SRR4302533.4398560/94‑1 (MQ=60)

GTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTC‑GCAAACGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTGCGTGCACCAGATCAATTTGAAATCCCGCC  >  NC_000913/3930584‑3930770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`