Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,931,938 T→C S179P (TCC→CCC)  rbsA → ATP‑binding component of D‑ribose high‑affinity transport system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,931,9380TC100.0% 797.2 / NA 251S179P (TCC→CCC) rbsAATP‑binding component of D‑ribose high‑affinity transport system
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (124/127);  total (124/127)

Only 100 of 267 total aligned reads displayed.

ATGGTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAATCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCGAGATTTGC  >  NC_000913/3931848‑3932027
                                                                                          |                                                                                         
aTGGTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAAccc                                                                                         >  2:2451408/1‑93 (MQ=255)
aTGGTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAAccc                                                                                         >  2:4366712/1‑93 (MQ=255)
 tGGTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAAcccc                                                                                        <  1:5169810/93‑1 (MQ=255)
 tGGTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAAcccc                                                                                        <  2:1310004/93‑1 (MQ=255)
  ggTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCt                                                                                       >  2:4506639/1‑93 (MQ=255)
  ggTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCt                                                                                       <  1:2514345/93‑1 (MQ=255)
   gTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTg                                                                                      <  1:6252388/93‑1 (MQ=255)
   gTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTg                                                                                      >  1:3951908/1‑93 (MQ=255)
   gTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTg                                                                                      <  2:2161615/93‑1 (MQ=255)
    ttGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGt                                                                                     >  2:3836751/1‑93 (MQ=255)
    ttGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGt                                                                                     <  2:2310071/93‑1 (MQ=255)
     tGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGtt                                                                                    <  2:188503/93‑1 (MQ=255)
        aaTCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTcgg                                                                                 >  1:6276051/1‑91 (MQ=255)
        aaTCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCcg                                                                                 >  2:1801981/1‑93 (MQ=255)
         aTCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCcgc                                                                                >  2:2637422/1‑93 (MQ=255)
            gCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTc                                                                             <  1:2335761/93‑1 (MQ=255)
            gCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTc                                                                             >  1:1043945/1‑93 (MQ=255)
             ccAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCa                                                                            >  2:4150656/1‑93 (MQ=255)
                aaGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATcc                                                                         >  1:1780851/1‑93 (MQ=255)
                  gTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCcgc                                                                       <  1:3740302/93‑1 (MQ=255)
                  gTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCcgc                                                                       <  1:6174031/93‑1 (MQ=255)
                     cTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAg                                                                    <  2:2988751/93‑1 (MQ=255)
                      tGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGc                                                                   >  2:3977457/1‑93 (MQ=255)
                      tGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGc                                                                   <  2:3739035/93‑1 (MQ=255)
                       gAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCt                                                                  <  2:5487195/93‑1 (MQ=255)
                         gCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGa                                                                <  2:4443348/93‑1 (MQ=255)
                            ttGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAAt                                                             <  2:1164710/93‑1 (MQ=255)
                              gAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCg                                                           >  2:3403642/1‑93 (MQ=255)
                               aGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGc                                                          <  2:5409933/93‑1 (MQ=255)
                                gTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCa                                                         >  1:1888550/1‑93 (MQ=255)
                                 tCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCaa                                                        >  1:1909709/1‑93 (MQ=255)
                                 tCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCaa                                                        <  2:2052472/93‑1 (MQ=255)
                                  cGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAg                                                       <  1:4216422/93‑1 (MQ=255)
                                  cGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAg                                                       <  1:2048366/93‑1 (MQ=255)
                                  cGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAg                                                       >  1:686701/1‑93 (MQ=255)
                                   gAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAgg                                                      <  2:5461087/93‑1 (MQ=255)
                                    aaaGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGc                                                     <  2:4222820/93‑1 (MQ=255)
                                    aaaGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGc                                                     <  2:2764751/93‑1 (MQ=255)
                                    aaaGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGc                                                     <  2:5956519/93‑1 (MQ=255)
                                     aaGTCATCCTTATGGATGATCCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGcc                                                    >  2:6110555/1‑93 (MQ=255)
                                     aaGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGcc                                                    <  2:1550907/93‑1 (MQ=255)
                                      aGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCcg                                                   >  1:5758343/1‑93 (MQ=255)
                                        tcatcaTTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCcgcg                                                 <  1:6120969/93‑1 (MQ=255)
                                         catcatTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCgg                                                <  1:418026/93‑1 (MQ=255)
                                          atcatTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGt                                               >  1:38598/1‑93 (MQ=255)
                                          atcatTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGt                                               <  1:6068733/93‑1 (MQ=255)
                                          atcatTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGt                                               <  2:4672917/93‑1 (MQ=255)
                                           tcatTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTa                                              >  2:708316/1‑93 (MQ=255)
                                           tcatTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTa                                              >  2:1572059/1‑93 (MQ=255)
                                            catTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTAt                                             <  1:2977807/93‑1 (MQ=255)
                                             atgaTGGATGAACCGACGGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTAtt                                            <  2:2917946/90‑1 (MQ=255)
                                               tATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGt                                          >  1:1408916/1‑93 (MQ=255)
                                                aTGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTc                                         <  2:3103701/93‑1 (MQ=255)
                                                aTGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTc                                         <  2:2382830/93‑1 (MQ=255)
                                                 tGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCt                                        >  1:1673305/1‑93 (MQ=255)
                                                  ggATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCta                                       <  1:5297378/93‑1 (MQ=255)
                                                   gATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTAGTGTCtat                                      >  1:3603183/1‑93 (MQ=255)
                                                    aTGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCtata                                     <  2:4873325/93‑1 (MQ=255)
                                                     tGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTatat                                    >  2:5761719/1‑93 (MQ=255)
                                                     tGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTatat                                    >  2:1763917/1‑93 (MQ=255)
                                                     tGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTatat                                    <  1:5048699/93‑1 (MQ=255)
                                                       aaCCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATAtct                                  <  2:4161485/93‑1 (MQ=255)
                                                        accgaccgATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATAtctc                                 >  2:3879418/1‑93 (MQ=255)
                                                         ccgaccgaTGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTcc                                >  1:1038739/1‑93 (MQ=255)
                                                          cgaccgaTGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTccc                               <  2:5041278/93‑1 (MQ=255)
                                                            gccaaTGCGCTGATCGATACCGAAACCGAACCCCTGTTCGTCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCAc                             <  1:5409885/89‑1 (MQ=255)
                                                            accgaTGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCAc                             <  1:1323652/93‑1 (MQ=255)
                                                            accgaTGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCAc                             >  1:851507/1‑93 (MQ=255)
                                                            accgaTGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCAc                             >  2:5577831/1‑93 (MQ=255)
                                                             ccgaTGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCAcc                            >  2:1428290/1‑93 (MQ=255)
                                                             ccgaTGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCAcc                            >  1:4952279/1‑93 (MQ=255)
                                                              cgaTGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGACGCGGTATTGTCTATATCTCCCACCg                           >  1:4698573/1‑93 (MQ=255)
                                                                aTGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCa                         >  2:4093870/1‑93 (MQ=255)
                                                                 tGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGGCGCGGGATTGTCTATATCTCCCACCGCAt                        >  2:795678/1‑93 (MQ=255)
                                                                 tGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCAt                        <  2:5361986/93‑1 (MQ=255)
                                                                   cgcTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATga                      <  1:1714875/93‑1 (MQ=255)
                                                                     cTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATgaaa                    >  1:1103255/1‑93 (MQ=255)
                                                                      tGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATgaaag                   >  1:4537011/1‑93 (MQ=255)
                                                                       gACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATgaaaga                  <  1:4434941/93‑1 (MQ=255)
                                                                       gACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATgaaaga                  >  2:2703786/1‑93 (MQ=255)
                                                                       gACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATgaaaga                  >  1:2939207/1‑93 (MQ=255)
                                                                       gACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATgaaaga                  <  2:1689920/93‑1 (MQ=255)
                                                                        aCCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATgaaagaa                 >  1:4627221/1‑93 (MQ=255)
                                                                           gATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATc              <  2:746199/93‑1 (MQ=255)
                                                                            aTACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCt             >  1:2742076/1‑93 (MQ=255)
                                                                            aTACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCt             <  1:1537368/93‑1 (MQ=255)
                                                                             tACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCtt            <  2:4942102/93‑1 (MQ=255)
                                                                             tACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCtt            <  1:553220/93‑1 (MQ=255)
                                                                             tACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCtt            >  1:77247/1‑93 (MQ=255)
                                                                              accgaaaccgaaCCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTc           <  1:4074616/93‑1 (MQ=255)
                                                                              accgaaaccgaaCCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTc           <  1:494163/93‑1 (MQ=255)
                                                                              accgaaaccgaaCCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCCCCCACCGCATGAAAGAAATCTTc           >  2:1977275/1‑93 (MQ=255)
                                                                                 gaaaccgaaCCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCgag        >  1:6062490/1‑93 (MQ=255)
                                                                                  aaaccgagCCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCgaga       <  1:2094533/93‑1 (MQ=255)
                                                                                  aaaccgaaCCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCgaga       <  2:3362152/93‑1 (MQ=255)
                                                                                     ccgaaCCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCGAGAttt    >  2:1247889/1‑93 (MQ=255)
                                                                                     ccgaaCCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTAGAGAttt    <  1:1817100/93‑1 (MQ=255)
                                                                                       gaaCCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCGAGATTTGc  >  1:3058579/1‑93 (MQ=255)
                                                                                       gaaCCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCGAGATTTGc  <  1:2705519/93‑1 (MQ=255)
                                                                                       gaaCCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCAACCGCATGAAAGAAATCTTCGAGATTTgg  >  1:2869735/1‑92 (MQ=255)
                                                                                          |                                                                                         
ATGGTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAATCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCGAGATTTGC  >  NC_000913/3931848‑3932027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAAATGGTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAATCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCGAGATTTGCGATG  >  NC_000913/3931845‑3932031
                                                                                             |                                                                                             
CAAATGGTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAAC                                                                                               <  SRR4302533.1862886/94‑1 (MQ=60)
CAAATGGTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAAC                                                                                               >  SRR4302533.5228589/1‑94 (MQ=60)
 AAATGGTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACC                                                                                              >  SRR4302533.5887999/1‑94 (MQ=60)
  AATGGTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCC                                                                                             <  SRR4302533.2427883/94‑1 (MQ=60)
    TGGTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCT                                                                                           <  SRR4302533.5201013/94‑1 (MQ=60)
    TGGTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCT                                                                                           <  SRR4302533.3811009/94‑1 (MQ=60)
     GGTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTG                                                                                          >  SRR4302533.2741079/1‑94 (MQ=60)
     GGTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTG                                                                                          <  SRR4302533.2529837/94‑1 (MQ=60)
     GGTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTG                                                                                          >  SRR4302533.3975998/1‑94 (MQ=60)
      GTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGT                                                                                         <  SRR4302533.6289788/94‑1 (MQ=60)
       TTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTT                                                                                        <  SRR4302533.1667205/94‑1 (MQ=60)
          AAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCGG                                                                                     >  SRR4302533.6313526/1‑94 (MQ=60)
          AAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCG                                                                                     >  SRR4302533.2903237/1‑94 (MQ=60)
           AATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGC                                                                                    >  SRR4302533.4745640/1‑94 (MQ=60)
              CGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTC                                                                                 >  SRR4302533.688443/1‑94 (MQ=60)
              CGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTC                                                                                 >  SRR4302533.1050081/1‑94 (MQ=60)
               GCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCA                                                                                <  SRR4302533.2350219/94‑1 (MQ=60)
               GCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCA                                                                                <  SRR4302533.4564223/94‑1 (MQ=60)
                 CAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATC                                                                              <  SRR4302533.1912638/94‑1 (MQ=60)
                 CAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCAGTTCCGCGTCATC                                                                              >  SRR4302533.5408222/1‑94 (MQ=60)
                  AAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCC                                                                             <  SRR4302533.466357/94‑1 (MQ=60)
                  AAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCC                                                                             <  SRR4302533.466356/94‑1 (MQ=60)
                  AAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCC                                                                             >  SRR4302533.1791690/1‑94 (MQ=60)
                     GTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCG                                                                          <  SRR4302533.3763241/94‑1 (MQ=60)
                     GTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCG                                                                          <  SRR4302533.6211134/94‑1 (MQ=60)
                      TGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGA                                                                         >  SRR4302533.5345099/1‑94 (MQ=60)
                        CTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGC                                                                       <  SRR4302533.1701714/94‑1 (MQ=60)
                         TGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCT                                                                      <  SRR4302533.5471394/94‑1 (MQ=60)
                         TGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCT                                                                      >  SRR4302533.121689/1‑94 (MQ=60)
                           AGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGA                                                                    >  SRR4302533.759835/1‑94 (MQ=60)
                              TTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAAT                                                                 >  SRR4302533.5826202/1‑94 (MQ=60)
                               TTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATC                                                                <  SRR4302533.4150706/94‑1 (MQ=60)
                                TGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCG                                                               >  SRR4302533.176680/1‑94 (MQ=60)
                                 GAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGC                                                              >  SRR4302533.6103130/1‑94 (MQ=60)
                                  AGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCA                                                             >  SRR4302533.1900088/1‑94 (MQ=60)
                                   GTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAA                                                            >  SRR4302533.1921360/1‑94 (MQ=60)
                                    TCGAAAGTCATCATTATGGATGACCCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAG                                                           <  SRR4302533.128863/94‑1 (MQ=60)
                                    TCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAG                                                           <  SRR4302533.3767833/94‑1 (MQ=60)
                                    TCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAG                                                           >  SRR4302533.690761/1‑94 (MQ=60)
                                    TCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAG                                                           <  SRR4302533.1874127/94‑1 (MQ=60)
                                    TCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAG                                                           <  SRR4302533.5295627/94‑1 (MQ=60)
                                     CGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATGCCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGG                                                          <  SRR4302533.1818847/94‑1 (MQ=60)
                                     CGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGG                                                          >  SRR4302533.2089501/1‑94 (MQ=60)
                                     CGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGG                                                          <  SRR4302533.2060996/94‑1 (MQ=60)
                                     CGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGG                                                          <  SRR4302533.4241928/94‑1 (MQ=60)
                                     CGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGC                                                          >  SRR4302533.549124/1‑94 (MQ=60)
                                      GAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGC                                                         <  SRR4302533.6230490/94‑1 (MQ=60)
                                      GAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGC                                                         >  SRR4302533.1095333/1‑94 (MQ=60)
                                      GAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGC                                                         >  SRR4302533.2990300/1‑94 (MQ=60)
                                       AAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCC                                                        >  SRR4302533.6330483/1‑94 (MQ=60)
                                       AAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCG‑GCTTGAATCGCAAGGCCC                                                       >  SRR4302533.2301650/1‑94 (MQ=60)
                                        AAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCG                                                       >  SRR4302533.5793093/1‑94 (MQ=60)
                                        AAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCG                                                       <  SRR4302533.6237197/94‑1 (MQ=60)
                                         AGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGC                                                      <  SRR4302533.2548732/94‑1 (MQ=60)
                                         AGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGC                                                      >  SRR4302533.1939857/1‑94 (MQ=60)
                                          GTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCG                                                     >  SRR4302533.1049341/1‑94 (MQ=60)
                                           TCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGG                                                    <  SRR4302533.3916465/94‑1 (MQ=60)
                                           TCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGG                                                    <  SRR4302533.6157879/94‑1 (MQ=60)
                                            CATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGT                                                   <  SRR4302533.420532/94‑1 (MQ=60)
                                            CATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGT                                                   >  SRR4302533.6166988/1‑94 (MQ=60)
                                            CATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGT                                                   >  SRR4302533.38852/1‑94 (MQ=60)
                                            CATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGT                                                   >  SRR4302533.1666918/1‑94 (MQ=60)
                                             ATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTA                                                  <  SRR4302533.6105454/94‑1 (MQ=60)
                                               CATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATT                                                <  SRR4302533.6193571/94‑1 (MQ=60)
                                               CATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATT                                                <  SRR4302533.2996026/94‑1 (MQ=60)
                                                ATTATGGATGAACCGACCGATGCGCTGACCGAGACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAAGCGCAAGGCCGCGGTATTG                                               <  SRR4302533.4478117/94‑1 (MQ=60)
                                                 TTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGT                                              >  SRR4302533.1417436/1‑94 (MQ=60)
                                                   ATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCT                                            <  SRR4302533.4347177/94‑1 (MQ=60)
                                                   ATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCT                                            >  SRR4302533.1683418/1‑94 (MQ=60)
                                                   ATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCT                                            <  SRR4302533.1776585/94‑1 (MQ=60)
                                                     GGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTAT                                          <  SRR4302533.5329106/94‑1 (MQ=60)
                                                     GGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTAGTGTCTAT                                          >  SRR4302533.3625360/1‑94 (MQ=60)
                                                      GATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATA                                         <  SRR4302533.3068606/94‑1 (MQ=60)
                                                       ATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATAT                                        <  SRR4302533.3996519/94‑1 (MQ=60)
                                                       ATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATAT                                        <  SRR4302533.4038094/94‑1 (MQ=60)
                                                       ATGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATAT                                        <  SRR4302533.5679535/94‑1 (MQ=60)
                                                        TGAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATC                                       <  SRR4302533.5079237/94‑1 (MQ=60)
                                                         GAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCT                                      >  SRR4302533.1484744/1‑94 (MQ=60)
                                                         GAACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCT                                      >  SRR4302533.6099790/1‑94 (MQ=60)
                                                           ACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCC                                    <  SRR4302533.239914/94‑1 (MQ=60)
                                                           ACCGACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCC                                    >  SRR4302533.1044843/1‑94 (MQ=60)
                                                              GACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCAC                                 >  SRR4302533.1919210/1‑94 (MQ=60)
                                                              GACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCAC                                 >  SRR4302533.856491/1‑94 (MQ=60)
                                                               gccaATGCGCTGATCGATACCGAAACCGAACCCCTGTTCGTCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACC                                <  SRR4302533.5442407/90‑1 (MQ=60)
                                                               ACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACC                                <  SRR4302533.1331590/94‑1 (MQ=60)
                                                               ACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACC                                >  SRR4302533.4982272/1‑94 (MQ=60)
                                                               ACCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACC                                >  SRR4302533.4982271/1‑94 (MQ=60)
                                                                CCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCG                               >  SRR4302533.6135875/1‑94 (MQ=60)
                                                                CCGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGACGCGGTATTGTCTATATCTCCCACCG                               >  SRR4302533.4727052/1‑94 (MQ=60)
                                                                 CGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGC                              <  SRR4302533.6303020/94‑1 (MQ=60)
                                                                 CGATGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGC                              >  SRR4302533.5075629/1‑94 (MQ=60)
                                                                    TGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATG                           <  SRR4302533.2490101/94‑1 (MQ=60)
                                                                    TGCGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATG                           <  SRR4302533.4975526/94‑1 (MQ=60)
                                                                      CGCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAA                         <  SRR4302533.1725274/94‑1 (MQ=60)
                                                                       GCTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAA                        >  SRR4302533.1109786/1‑94 (MQ=60)
                                                                        CTGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAG                       >  SRR4302533.4564479/1‑94 (MQ=60)
                                                                         TGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTGTATCTCCCACCGCATGAAAGA                      >  SRR4302533.3052622/1‑94 (MQ=60)
                                                                         TGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGA                      >  SRR4302533.992259/1‑94 (MQ=60)
                                                                         TGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGA                      <  SRR4302533.1466614/94‑1 (MQ=60)
                                                                         TGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGA                      >  SRR4302533.3675797/1‑94 (MQ=60)
                                                                         TGACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGA                      >  SRR4302533.2957165/1‑94 (MQ=60)
                                                                          GACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAGTCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAA                     >  SRR4302533.4615638/1‑94 (MQ=60)
                                                                          GACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAA                     <  SRR4302533.1795126/94‑1 (MQ=60)
                                                                          GACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAA                     <  SRR4302533.4461750/94‑1 (MQ=60)
                                                                          GACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAA                     <  SRR4302533.435430/94‑1 (MQ=60)
                                                                          GACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAA                     <  SRR4302533.1370935/94‑1 (MQ=60)
                                                                          GACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAA                     <  SRR4302533.4228869/94‑1 (MQ=60)
                                                                          GACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAA                     >  SRR4302533.4655265/1‑94 (MQ=60)
                                                                           ACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAA                    >  SRR4302533.955697/1‑94 (MQ=60)
                                                                           ACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAA                    >  SRR4302533.2177408/1‑94 (MQ=60)
                                                                           ACCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAA                    <  SRR4302533.2972822/94‑1 (MQ=60)
                                                                            CCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAAT                   >  SRR4302533.5094413/1‑94 (MQ=60)
                                                                            CCGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAAT                   <  SRR4302533.775132/94‑1 (MQ=60)
                                                                             CGATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATC                  >  SRR4302533.5821746/1‑94 (MQ=60)
                                                                              GATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCT                 <  SRR4302533.3112993/94‑1 (MQ=60)
                                                                              GATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCT                 <  SRR4302533.4888216/94‑1 (MQ=60)
                                                                              GATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCT                 >  SRR4302533.2758889/1‑94 (MQ=60)
                                                                               ATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTT                >  SRR4302533.77745/1‑94 (MQ=60)
                                                                               ATACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTT                <  SRR4302533.1546603/94‑1 (MQ=60)
                                                                                TACCGAAACCGACCCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTC               <  SRR4302533.2825620/94‑1 (MQ=60)
                                                                                TACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTC               <  SRR4302533.2825618/94‑1 (MQ=60)
                                                                                TACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTC               <  SRR4302533.556500/94‑1 (MQ=60)
                                                                                 ACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCG              <  SRR4302533.807227/94‑1 (MQ=60)
                                                                                 ACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCG              <  SRR4302533.497111/94‑1 (MQ=60)
                                                                                 ACCGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCG              <  SRR4302533.4099422/94‑1 (MQ=60)
                                                                                   CGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCGAG            >  SRR4302533.6099190/1‑94 (MQ=60)
                                                                                   CGAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCGAG            >  SRR4302533.5143551/1‑94 (MQ=60)
                                                                                    GAAACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCGAGA           >  SRR4302533.2736/1‑94 (MQ=60)
                                                                                     AAACCGAGCCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCGAGAT          <  SRR4302533.2107431/94‑1 (MQ=60)
                                                                                       ACCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCGAGATTT        <  SRR4302533.3732659/94‑1 (MQ=60)
                                                                                        CCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCGAGATTTG       <  SRR4302533.4203811/94‑1 (MQ=60)
                                                                                        CCGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTAGAGATTTG       <  SRR4302533.1828193/94‑1 (MQ=60)
                                                                                         CGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCGAGATTTGC      >  SRR4302533.3077347/1‑94 (MQ=60)
                                                                                         CGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCGAGATTTGC      >  SRR4302533.1093572/1‑94 (MQ=60)
                                                                                         CGAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCAACCGCATGAAAGAAATCTTCGAGATTTGG      >  SRR4302533.2887393/1‑94 (MQ=60)
                                                                                          GAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCGAGATTTGCG     >  SRR4302533.2826441/1‑94 (MQ=60)
                                                                                          GAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCGAGATTTGCG     >  SRR4302533.2073250/1‑94 (MQ=60)
                                                                                          GAACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCGAGATTTGCG     <  SRR4302533.2722113/94‑1 (MQ=60)
                                                                                           AACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCGAGATTTGCGA    >  SRR4302533.3060558/1‑94 (MQ=60)
                                                                                            ACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCTGTATTGTCTATATCTCCCTCCGCATGAAAGAAATCTTCGAGATTTGCGAT   <  SRR4302533.5221258/94‑1 (MQ=60)
                                                                                            ACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCGAGATTTGCGAT   <  SRR4302533.3702780/94‑1 (MQ=60)
                                                                                            ACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCGAGATTTGCGAT   <  SRR4302533.2361363/94‑1 (MQ=60)
                                                                                            ACCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCGAGATTTGCGAT   >  SRR4302533.4618333/1‑94 (MQ=60)
                                                                                             CCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCGAGATTTGCGATG  >  SRR4302533.3419955/1‑94 (MQ=60)
                                                                                             |                                                                                             
CAAATGGTTGAAATCGCCAAAGTGCTGAGCTTTGAGTCGAAAGTCATCATTATGGATGAACCGACCGATGCGCTGACCGATACCGAAACCGAATCCCTGTTCCGCGTCATCCGCGAGCTGAAATCGCAAGGCCGCGGTATTGTCTATATCTCCCACCGCATGAAAGAAATCTTCGAGATTTGCGATG  >  NC_000913/3931845‑3932031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑