Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,944,676 2 bp→GC intergenic (+20/+75) trpT → / ← yifDA tRNA‑Trp/transcription regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,944,6760CG100.0% 762.8 / NA 234intergenic (+20/+76)trpT/yifDAtRNA‑Trp/transcription regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (106/128);  total (106/128)
*NC_0009133,944,6770GC100.0% 765.4 / NA 234intergenic (+21/+75)trpT/yifDAtRNA‑Trp/transcription regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (106/128);  total (106/128)

Only 100 of 236 total aligned reads displayed.

AGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAACGTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTC  >  NC_000913/3944589‑3944763
                                                                                       ||                                                                                      
  ttCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGa                                                                                  >  1:4546057/1‑93 (MQ=255)
  ttCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGa                                                                                  <  2:2908602/93‑1 (MQ=255)
  ttCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGa                                                                                  >  2:5367252/1‑93 (MQ=255)
  ttCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGa                                                                                  >  1:1455093/1‑93 (MQ=255)
  ttCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGa                                                                                  <  1:1654124/93‑1 (MQ=255)
  ttCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCCCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGa                                                                                  <  1:417566/93‑1 (MQ=255)
   tCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGAt                                                                                 <  2:939572/93‑1 (MQ=255)
    cAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGAtt                                                                                <  1:4310168/93‑1 (MQ=255)
      aTTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGAtttt                                                                              <  2:4211956/93‑1 (MQ=255)
      aTTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGAtttt                                                                              <  1:2296771/93‑1 (MQ=255)
      aTTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGGCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGAtttt                                                                              >  1:473677/1‑93 (MQ=255)
        tGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGAtttttt                                                                            <  1:289855/93‑1 (MQ=255)
           tAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTa                                                                         <  1:6137468/93‑1 (MQ=255)
           tAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTa                                                                         <  1:5366087/93‑1 (MQ=255)
           tAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTa                                                                         <  2:2538204/93‑1 (MQ=255)
           tAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTa                                                                         <  1:1227938/93‑1 (MQ=255)
           tAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTa                                                                         >  1:4688443/1‑93 (MQ=255)
           tAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTa                                                                         <  2:6141562/93‑1 (MQ=255)
             gagCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATc                                                                       <  2:5583936/93‑1 (MQ=255)
             gagCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATc                                                                       <  1:4076334/93‑1 (MQ=255)
             gagCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATc                                                                       <  1:1878858/93‑1 (MQ=255)
                cACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGa                                                                    <  2:1343653/93‑1 (MQ=255)
                 aCCGGTCTACAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGaa                                                                   >  2:4396873/1‑93 (MQ=255)
                  ccGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGaaa                                                                  >  2:4253607/1‑93 (MQ=255)
                        tcCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAAccc                                                            >  2:361362/1‑93 (MQ=255)
                         ccAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCct                                                           <  1:3936384/93‑1 (MQ=255)
                         ccAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCct                                                           <  1:1691185/93‑1 (MQ=255)
                         ccAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCct                                                           <  2:300649/93‑1 (MQ=255)
                         ccAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCct                                                           >  2:3345310/1‑93 (MQ=255)
                         ccAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCct                                                           <  1:1408270/93‑1 (MQ=255)
                          cAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCctc                                                          <  2:834202/93‑1 (MQ=255)
                          cAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCctc                                                          >  2:58721/1‑93 (MQ=255)
                           aaaaCCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCctct                                                         >  2:1124982/1‑93 (MQ=255)
                           aaaaCCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCctct                                                         >  2:4335178/1‑93 (MQ=255)
                           aaaaCCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCctct                                                         >  1:5999192/1‑93 (MQ=255)
                            aaaCCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCtctc                                                        >  1:4351292/1‑93 (MQ=255)
                            aaaCCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCtctc                                                        <  1:3834398/93‑1 (MQ=255)
                            aaaCCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCtctc                                                        <  2:401779/93‑1 (MQ=255)
                            aaaCCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCtctc                                                        <  2:6283096/93‑1 (MQ=255)
                              aCCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCg                                                      >  2:3117538/1‑93 (MQ=255)
                               ccGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGa                                                     <  2:4553859/93‑1 (MQ=255)
                                cGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGaa                                                    >  2:2176743/1‑93 (MQ=255)
                                    tgtTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTaa                                                <  2:2911891/93‑1 (MQ=255)
                                     gtTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTaaa                                               >  1:5471791/1‑93 (MQ=255)
                                      ttGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAACTAAAt                                              >  1:163990/1‑93 (MQ=255)
                                        gggAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATcc                                            <  1:3153838/93‑1 (MQ=255)
                                        gggAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATcc                                            >  2:6203288/1‑93 (MQ=255)
                                         ggAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCt                                           >  1:4890219/1‑93 (MQ=255)
                                          gAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCtt                                          <  1:4389792/93‑1 (MQ=255)
                                           aGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTc                                         <  1:341987/93‑1 (MQ=255)
                                            gTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCt                                        >  1:2270768/1‑93 (MQ=255)
                                              tCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTAc                                      <  2:652619/93‑1 (MQ=255)
                                              tCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTAc                                      >  2:4112574/1‑93 (MQ=255)
                                               cGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTAcc                                     <  1:1756372/93‑1 (MQ=255)
                                               cGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTAcc                                     <  1:5871781/93‑1 (MQ=255)
                                                gAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCg                                    >  1:5032534/1‑93 (MQ=255)
                                                gAGTCACTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATATCCCTCTCCGAAGTAAATCCTTCTACCg                                    <  2:751524/93‑1 (MQ=255)
                                                  gTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGc                                  >  2:6012460/1‑93 (MQ=255)
                                                  gTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGc                                  <  1:2126441/93‑1 (MQ=255)
                                                   tctctcCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCa                                 <  1:3471501/93‑1 (MQ=255)
                                                     tctcCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATc                               >  2:1999424/1‑93 (MQ=255)
                                                     tctcCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACACTCTCCGAAGTAAATCCTTCTACAGGCATc                               >  2:3388778/1‑93 (MQ=255)
                                                       tcCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCt                             >  1:1172680/1‑93 (MQ=255)
                                                       tcCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCt                             <  1:5118532/93‑1 (MQ=255)
                                                        ccGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCtt                            <  1:5000832/93‑1 (MQ=255)
                                                         cGCCCCTGCCAGAAATCATCCTTAGCGAAGGCTAAGGATTTTTTTTATCTGAAATAATCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTg                           >  1:3353308/1‑93 (MQ=255)
                                                         cGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTg                           >  1:1992686/1‑93 (MQ=255)
                                                         cGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTg                           <  1:639758/93‑1 (MQ=255)
                                                          gCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgc                          <  2:2603191/93‑1 (MQ=255)
                                                          gCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgc                          >  1:5649179/1‑93 (MQ=255)
                                                           ccccTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgcc                         <  2:5003381/93‑1 (MQ=255)
                                                           ccccTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgcc                         >  2:3507328/1‑93 (MQ=255)
                                                            cccTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgcca                        >  2:4776353/1‑93 (MQ=255)
                                                            cccTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgcca                        >  2:4792811/1‑93 (MQ=255)
                                                            cccTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgcca                        >  1:2413578/1‑93 (MQ=255)
                                                            cccTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgcca                        >  1:966436/1‑93 (MQ=255)
                                                            cccTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgcca                        >  1:5156058/1‑93 (MQ=255)
                                                             ccTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgccag                       >  2:377313/1‑93 (MQ=255)
                                                              cTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgccagc                      >  2:1045683/1‑93 (MQ=255)
                                                                gCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgccagcca                    >  2:3992521/1‑93 (MQ=255)
                                                                gCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgccagcca                    >  1:1927242/1‑93 (MQ=255)
                                                                     aaaTCATCCTAAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCat               <  1:2800363/93‑1 (MQ=255)
                                                                      aaTCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCata              >  1:5803502/1‑93 (MQ=255)
                                                                      aaTCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCata              <  1:3700403/93‑1 (MQ=255)
                                                                      aaTCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCata              >  2:4635310/1‑93 (MQ=255)
                                                                      aaTCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCata              <  1:820189/93‑1 (MQ=255)
                                                                      aaTCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCata              <  2:3034914/93‑1 (MQ=255)
                                                                       atcatcCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCatat             >  1:1062629/1‑93 (MQ=255)
                                                                       atcatcCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCatat             <  1:3001894/93‑1 (MQ=255)
                                                                         catcCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTa           >  1:3521997/1‑93 (MQ=255)
                                                                         catcCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTa           >  2:3294146/1‑93 (MQ=255)
                                                                         catcCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTa           <  1:5218894/93‑1 (MQ=255)
                                                                          atcCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTaa          >  2:1191901/1‑93 (MQ=255)
                                                                          atcCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTaa          >  2:684487/1‑93 (MQ=255)
                                                                           tcCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAAt         >  1:1288474/1‑93 (MQ=255)
                                                                            ccTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATACCCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATa        <  2:581723/93‑1 (MQ=255)
                                                                             cTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATac       >  1:2552647/1‑93 (MQ=255)
                                                                             cTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATac       >  1:5584229/1‑93 (MQ=255)
                                                                                aGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACt    <  1:3028555/93‑1 (MQ=255)
                                                                                  cGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTc  <  2:2115258/93‑1 (MQ=255)
                                                                                       ||                                                                                      
AGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAACGTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTC  >  NC_000913/3944589‑3944763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAACGTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCAT  >  NC_000913/3944583‑3944765
                                                                                             |                                                                                         
GGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAG                                                                                           >  SRR4302533.3824063/1‑94 (MQ=60)
GGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAG                                                                                           <  SRR4302533.5779589/94‑1 (MQ=60)
     TAGTTCAATTGGTAGAGCACCGTTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAG                                                                                      <  SRR4302533.2049403/94‑1 (MQ=60)
     TAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAG                                                                                      <  SRR4302533.255971/94‑1 (MQ=60)
     TAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAG                                                                                      <  SRR4302533.5684009/94‑1 (MQ=60)
     TAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAG                                                                                      <  SRR4302533.6332962/94‑1 (MQ=60)
     TAATTCAATCGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAG                                                                                      <  SRR4302533.1753055/94‑1 (MQ=60)
      AGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGG                                                                                     >  SRR4302533.6121653/1‑94 (MQ=60)
      AGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGG                                                                                     <  SRR4302533.3550263/94‑1 (MQ=60)
      AGTTAAATTGGTAGAGCACTGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGG                                                                                     <  SRR4302533.1432829/94‑1 (MQ=60)
       GTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGA                                                                                    <  SRR4302533.3707087/94‑1 (MQ=60)
       GTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGA                                                                                    >  SRR4302533.1463866/1‑94 (MQ=60)
       GTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGA                                                                                    >  SRR4302533.4573590/1‑94 (MQ=60)
        TTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGAT                                                                                   <  SRR4302533.1664139/94‑1 (MQ=60)
        TTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCCCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGAT                                                                                   <  SRR4302533.420071/94‑1 (MQ=60)
         TCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATT                                                                                  <  SRR4302533.663524/94‑1 (MQ=60)
          CAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTT                                                                                 <  SRR4302533.4336114/94‑1 (MQ=60)
          CAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTT                                                                                 <  SRR4302533.5862518/94‑1 (MQ=60)
           AATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTT                                                                                <  SRR4302533.3128439/94‑1 (MQ=60)
           AATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGGCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTT                                                                                >  SRR4302533.476507/1‑94 (MQ=60)
            ATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTT                                                                               <  SRR4302533.2310996/94‑1 (MQ=60)
             TTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTT                                                                              <  SRR4302533.6295751/94‑1 (MQ=60)
              TGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTT                                                                             <  SRR4302533.291621/94‑1 (MQ=60)
                GTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTA                                                                           >  SRR4302533.4716863/1‑94 (MQ=60)
                 TAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTAT                                                                          <  SRR4302533.6174441/94‑1 (MQ=60)
                 TAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTAT                                                                          <  SRR4302533.5398321/94‑1 (MQ=60)
                 TAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTAT                                                                          <  SRR4302533.1235291/94‑1 (MQ=60)
                  AGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATC                                                                         >  SRR4302533.5911517/1‑94 (MQ=60)
                   GAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCT                                                                        <  SRR4302533.4101147/94‑1 (MQ=60)
                   GAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCT                                                                        <  SRR4302533.1890308/94‑1 (MQ=60)
                      CACCGGTCTCCGAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAA                                                                     <  SRR4302533.3454084/94‑1 (MQ=60)
                       CCCGGTCTCCAAACCCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAA                                                                    <  SRR4302533.372293/94‑1 (MQ=60)
                       ACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAA                                                                    >  SRR4302533.1228046/1‑94 (MQ=60)
                        CCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAAT                                                                   >  SRR4302533.3525525/1‑94 (MQ=60)
                        CCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAAT                                                                   <  SRR4302533.5260845/94‑1 (MQ=60)
                         CGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATA                                                                  <  SRR4302533.2989523/94‑1 (MQ=60)
                           GTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAAC                                                                <  SRR4302533.444911/94‑1 (MQ=60)
                           GTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAAC                                                                >  SRR4302533.438966/1‑94 (MQ=60)
                            TCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACC                                                               <  SRR4302533.717061/94‑1 (MQ=60)
                             CTCCAAAACCGGGTGTTGGTAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCC                                                              <  SRR4302533.5410296/94‑1 (MQ=60)
                             CTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCC                                                              <  SRR4302533.1903323/94‑1 (MQ=60)
                              TCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCT                                                             <  SRR4302533.1267096/94‑1 (MQ=60)
                               CCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTC                                                            <  SRR4302533.1701429/94‑1 (MQ=60)
                               CCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTC                                                            <  SRR4302533.1416783/94‑1 (MQ=60)
                               CCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTC                                                            <  SRR4302533.3960414/94‑1 (MQ=60)
                               CCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTC                                                            <  SRR4302533.2001323/94‑1 (MQ=60)
                                CAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCT                                                           >  SRR4302533.419592/1‑94 (MQ=60)
                                CAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCT                                                           <  SRR4302533.4574212/94‑1 (MQ=60)
                                CAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCT                                                           >  SRR4302533.6035529/1‑94 (MQ=60)
                                 AAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTC                                                          >  SRR4302533.4377549/1‑94 (MQ=60)
                                  AAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCC                                                         <  SRR4302533.3857830/94‑1 (MQ=60)
                                  AAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCC                                                         >  SRR4302533.3943623/1‑94 (MQ=60)
                                  AAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCC                                                         >  SRR4302533.2236302/1‑94 (MQ=60)
                                  AAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAAACCTCTTC                                                         >  SRR4302533.5946224/1‑94 (MQ=60)
                                   AACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCG                                                        <  SRR4302533.5663935/94‑1 (MQ=60)
                                          TGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAA                                                 >  SRR4302533.5504766/1‑94 (MQ=60)
                                           GTTGGGAGTTCGAGTCTCTCCTCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAAT                                                >  SRR4302533.5545289/1‑94 (MQ=60)
                                           GTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAAT                                                <  SRR4302533.165673/94‑1 (MQ=60)
                                           GTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAACTAAAT                                                >  SRR4302533.165033/1‑94 (MQ=60)
                                             TGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCC                                              >  SRR4302533.4777086/1‑94 (MQ=60)
                                              GGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCT                                             <  SRR4302533.4127297/94‑1 (MQ=60)
                                              GGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCT                                             <  SRR4302533.3173226/94‑1 (MQ=60)
                                              GGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCT                                             <  SRR4302533.3035700/94‑1 (MQ=60)
                                              GGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCT                                             >  SRR4302533.4919848/1‑94 (MQ=60)
                                               GGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTT                                            <  SRR4302533.3186806/94‑1 (MQ=60)
                                                GAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTC                                           <  SRR4302533.3478028/94‑1 (MQ=60)
                                                GAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTC                                           <  SRR4302533.1759918/94‑1 (MQ=60)
                                                GAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTC                                           <  SRR4302533.4416297/94‑1 (MQ=60)
                                                 GGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCT                                          <  SRR4302533.2374335/94‑1 (MQ=60)
                                                 AGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCT                                          >  SRR4302533.2284819/1‑94 (MQ=60)
                                                 AGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCT                                          <  SRR4302533.344075/94‑1 (MQ=60)
                                                 AGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCT                                          <  SRR4302533.4750192/94‑1 (MQ=60)
                                                  GTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTA                                         >  SRR4302533.2307451/1‑94 (MQ=60)
                                                   TTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTAC                                        <  SRR4302533.2534068/94‑1 (MQ=60)
                                                     CGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCG                                      <  SRR4302533.1767071/94‑1 (MQ=60)
                                                     CGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCG                                      <  SRR4302533.5907275/94‑1 (MQ=60)
                                                     CGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCG                                      >  SRR4302533.2448007/1‑94 (MQ=60)
                                                     CGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCG                                      >  SRR4302533.5063022/1‑94 (MQ=60)
                                                      GAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGG                                     >  SRR4302533.1712178/1‑94 (MQ=60)
                                                        GTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCA                                   <  SRR4302533.2139550/94‑1 (MQ=60)
                                                         TCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCAT                                  <  SRR4302533.3492927/94‑1 (MQ=60)
                                                          CTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATC                                 <  SRR4302533.4029049/94‑1 (MQ=60)
                                                          CTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATC                                 >  SRR4302533.1528812/1‑94 (MQ=60)
                                                            CTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCT                               >  SRR4302533.1179719/1‑94 (MQ=60)
                                                             TCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTT                              <  SRR4302533.5167629/94‑1 (MQ=60)
                                                             TCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTT                              <  SRR4302533.5149458/94‑1 (MQ=60)
                                                             TCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTT                              <  SRR4302533.2963064/94‑1 (MQ=60)
                                                              CCGCCCCTGCCAGAAATCATCCTTAGCGAAGGCTAAGGATTTTTTTTATCTGAAATAATCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTG                             >  SRR4302533.3374027/1‑94 (MQ=60)
                                                              CCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTG                             <  SRR4302533.5031145/94‑1 (MQ=60)
                                                              CCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTG                             >  SRR4302533.2004906/1‑94 (MQ=60)
                                                               CGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGC                            >  SRR4302533.1573828/1‑94 (MQ=60)
                                                               CGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGC                            >  SRR4302533.5064949/1‑94 (MQ=60)
                                                               CGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGC                            >  SRR4302533.1691925/1‑94 (MQ=60)
                                                               CGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGC                            <  SRR4302533.643522/94‑1 (MQ=60)
                                                               CGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGC                            >  SRR4302533.5683330/1‑94 (MQ=60)
                                                                 CCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCA                          >  SRR4302533.5187191/1‑94 (MQ=60)
                                                                 CCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCA                          >  SRR4302533.972106/1‑94 (MQ=60)
                                                                 CCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCA                          >  SRR4302533.2428490/1‑94 (MQ=60)
                                                                  CCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAG                         <  SRR4302533.5815223/94‑1 (MQ=60)
                                                                  CCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAC                         >  SRR4302533.5163889/1‑94 (MQ=60)
                                                                   CCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGC                        >  SRR4302533.1969510/1‑94 (MQ=60)
                                                                    CTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCC                       >  SRR4302533.433253/1‑94 (MQ=60)
                                                                     TGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCA                      >  SRR4302533.1939003/1‑94 (MQ=60)
                                                                        CAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTC                   >  SRR4302533.5463291/1‑94 (MQ=60)
                                                                         AGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATATCCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCA                  <  SRR4302533.4957139/94‑1 (MQ=60)
                                                                         AGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCA                  <  SRR4302533.3823622/94‑1 (MQ=60)
                                                                         AGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCA                  <  SRR4302533.1126534/94‑1 (MQ=60)
                                                                         AGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCA                  >  SRR4302533.3191/1‑94 (MQ=60)
                                                                          GAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCAT                 <  SRR4302533.5734526/94‑1 (MQ=60)
                                                                          GAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCAT                 <  SRR4302533.2848557/94‑1 (MQ=60)
                                                                           AAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATA                >  SRR4302533.5838555/1‑94 (MQ=60)
                                                                           AAATCATCCTAAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATA                <  SRR4302533.2817586/94‑1 (MQ=60)
                                                                            AATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATAT               <  SRR4302533.824997/94‑1 (MQ=60)
                                                                            AATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATAT               <  SRR4302533.4749312/94‑1 (MQ=60)
                                                                            AATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATAT               >  SRR4302533.1068874/1‑94 (MQ=60)
                                                                            AATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATAT               >  SRR4302533.5040347/1‑94 (MQ=60)
                                                                            AATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATAT               <  SRR4302533.3723130/94‑1 (MQ=60)
                                                                            AATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATAT               <  SRR4302533.5221098/94‑1 (MQ=60)
                                                                             ATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATT              <  SRR4302533.3020252/94‑1 (MQ=60)
                                                                              TCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTA             >  SRR4302533.3543706/1‑94 (MQ=60)
                                                                               CATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAA            >  SRR4302533.201276/1‑94 (MQ=60)
                                                                               CATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAA            >  SRR4302533.825455/1‑94 (MQ=60)
                                                                               CATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAA            >  SRR4302533.4352887/1‑94 (MQ=60)
                                                                               CATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAA            <  SRR4302533.5250305/94‑1 (MQ=60)
                                                                                ATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAAT           >  SRR4302533.1296228/1‑94 (MQ=60)
                                                                                 TCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATA          >  SRR4302533.4410505/1‑94 (MQ=60)
                                                                                  CCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATAC         >  SRR4302533.2568353/1‑94 (MQ=60)
                                                                                  CCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATAC         <  SRR4302533.4981232/94‑1 (MQ=60)
                                                                                  CCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATAC         >  SRR4302533.5617969/1‑94 (MQ=60)
                                                                                  CCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCACCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATAC         >  SRR4302533.3400520/1‑94 (MQ=60)
                                                                                   CTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACA        >  SRR4302533.2204107/1‑94 (MQ=60)
                                                                                   CTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACA        >  SRR4302533.2658932/1‑94 (MQ=60)
                                                                                    TTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACAC       >  SRR4302533.1777172/1‑94 (MQ=60)
                                                                                     TAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACT      >  SRR4302533.140626/1‑94 (MQ=60)
                                                                                     TAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACT      <  SRR4302533.1243991/94‑1 (MQ=60)
                                                                                      AGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTT     <  SRR4302533.3047088/94‑1 (MQ=60)
                                                                                        CGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACTCTTCA   >  SRR4302533.5178747/1‑94 (MQ=60)
                                                                                        CGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCA   >  SRR4302533.1107604/1‑94 (MQ=60)
                                                                                        CGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCA   >  SRR4302533.3043559/1‑94 (MQ=60)
                                                                                        CGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCA   >  SRR4302533.2280830/1‑94 (MQ=60)
                                                                                         GAAAGCTAAGTATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCAT  <  SRR4302533.1575049/94‑1 (MQ=60)
                                                                                             |                                                                                         
GGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAACGTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCAT  >  NC_000913/3944583‑3944765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑