Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,951,082 C→T intergenic (+46/‑50) ilvE → / → ilvD branched‑chain amino‑acid aminotransferase/dihydroxyacid dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,951,0820CT100.0% 943.6 / NA 253intergenic (+46/‑50)ilvE/ilvDbranched‑chain amino‑acid aminotransferase/dihydroxyacid dehydratase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (129/124);  total (129/124)

Only 100 of 266 total aligned reads displayed.

GAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGGCCCA  >  NC_000913/3950992‑3951171
                                                                                          |                                                                                         
gAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGatac                                                                                         >  2:360541/1‑93 (MQ=255)
 aaaCCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGatacg                                                                                        <  2:1324327/93‑2 (MQ=255)
 aaaCCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACt                                                                                        <  2:2291052/93‑1 (MQ=255)
  aaCCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTg                                                                                       >  1:2397468/1‑93 (MQ=255)
    ccGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTggg                                                                                     <  1:5130240/93‑1 (MQ=255)
    ccGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTggg                                                                                     <  1:2617084/93‑1 (MQ=255)
     cGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATATAAAAAATAGGACGGTAAGCACCGACCCATTTACGAGACAGATACTGGGa                                                                                    >  2:6164120/1‑93 (MQ=255)
     cGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGa                                                                                    >  1:1031390/1‑93 (MQ=255)
     cGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGa                                                                                    <  1:3807318/93‑1 (MQ=255)
     cGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGa                                                                                    >  2:3482049/1‑93 (MQ=255)
     cGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGa                                                                                    <  1:1394885/93‑1 (MQ=255)
      gAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAg                                                                                   >  2:6031654/1‑93 (MQ=255)
      gAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAg                                                                                   >  2:2474731/1‑93 (MQ=255)
      gAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAg                                                                                   <  1:5660903/93‑1 (MQ=255)
       aaGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGt                                                                                  >  1:3720646/1‑93 (MQ=255)
        aGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGta                                                                                 <  1:151405/93‑1 (MQ=255)
        aGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGta                                                                                 <  1:3031192/93‑1 (MQ=255)
        aGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGAACGCACCGTCCCATTTACGAGACAGATACTGGGAGta                                                                                 >  2:2009633/1‑93 (MQ=255)
         gATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGtaa                                                                                >  1:126409/1‑93 (MQ=255)
          aTAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGtaaa                                                                               <  1:142078/93‑1 (MQ=255)
            aaaTGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGtaaata                                                                             <  1:5344783/93‑1 (MQ=255)
            aaaTGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGtaaata                                                                             <  1:4774264/93‑1 (MQ=255)
             aaTGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGtaaataa                                                                            >  1:2377316/1‑93 (MQ=255)
             aaTGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGtaaataa                                                                            >  1:4085846/1‑93 (MQ=255)
              aTGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGtaaataaa                                                                           >  1:3958845/1‑93 (MQ=255)
               tGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAg                                                                          <  2:2742883/93‑1 (MQ=255)
                 gggCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTa                                                                        <  1:2258593/93‑1 (MQ=255)
                 gggCTGGTGAGATCAAGTTAATCAATAAATACAAAAAAGGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTa                                                                        <  1:6232801/93‑1 (MQ=255)
                  ggCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTAt                                                                       >  2:864152/1‑93 (MQ=255)
                     tGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGcc                                                                    <  1:2177958/93‑1 (MQ=255)
                      ggTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCt                                                                   >  1:5512528/1‑93 (MQ=255)
                       gTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTa                                                                  >  1:1273204/1‑93 (MQ=255)
                        ttAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTaa                                                                 <  2:363875/93‑1 (MQ=255)
                        ttAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTaa                                                                 >  1:3882550/1‑93 (MQ=255)
                        ttAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTaa                                                                 <  1:5392397/93‑1 (MQ=255)
                        ttAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTaa                                                                 <  1:4441099/93‑1 (MQ=255)
                         tAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAg                                                                <  1:4077427/93‑1 (MQ=255)
                         tAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAg                                                                <  2:4161504/93‑1 (MQ=255)
                          aGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGt                                                               >  2:3409745/1‑93 (MQ=255)
                          aGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGt                                                               <  2:4413448/93‑1 (MQ=255)
                           gATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTa                                                              >  2:637234/1‑93 (MQ=255)
                            aTCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTAc                                                             <  1:3496439/93‑1 (MQ=255)
                             tCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTAcc                                                            <  1:4331944/93‑1 (MQ=255)
                               aaGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGt                                                          >  1:3237234/1‑93 (MQ=255)
                               aaGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGt                                                          <  2:4680957/93‑1 (MQ=255)
                                aGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATGCTGGGAGTAAATAAAGTATGCCTAAGTACCGtt                                                         <  1:5710967/93‑1 (MQ=255)
                                 gTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTc                                                        >  2:5582444/1‑93 (MQ=255)
                                 attAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTc                                                        <  2:3789538/92‑1 (MQ=255)
                                  ttAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTcc                                                       <  2:1665950/93‑1 (MQ=255)
                                     aTCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGcc                                                    <  2:5656857/93‑1 (MQ=255)
                                     aTCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGcc                                                    >  2:5831966/1‑93 (MQ=255)
                                       cAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGccac                                                  >  2:5106264/1‑93 (MQ=255)
                                        aataaataCAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGccacc                                                 >  2:277203/1‑93 (MQ=255)
                                         ataaataCAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGccacca                                                >  1:1713460/1‑93 (MQ=255)
                                           aaataCAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGccaccacc                                              <  1:6043810/93‑1 (MQ=255)
                                            aataCAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGccaccacca                                             >  2:54393/1‑93 (MQ=255)
                                            aataCAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGccaccacca                                             >  2:3988964/1‑93 (MQ=255)
                                             ataCAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCcaccaccac                                            <  1:4043762/93‑1 (MQ=255)
                                              taCAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACt                                           <  2:2350376/93‑1 (MQ=255)
                                               aCAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTc                                          <  1:1654172/93‑1 (MQ=255)
                                                   aaaaTGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATgg                                      <  2:5332162/93‑1 (MQ=255)
                                                    aaaTGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCCCTCATGGt                                     <  2:1892706/93‑1 (MQ=255)
                                                    aaaTGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGt                                     <  1:734441/93‑1 (MQ=255)
                                                    aaaTGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGt                                     >  1:204746/1‑93 (MQ=255)
                                                     aaTGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTc                                    >  1:85883/1‑93 (MQ=255)
                                                      aTGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCg                                   <  2:1997416/93‑1 (MQ=255)
                                                       tGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCga                                  >  1:1952588/1‑92 (MQ=255)
                                                         gggcGGCACGCACCGTCCCAGTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTaa                                <  1:1062593/90‑1 (MQ=255)
                                                          gACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAat                               >  2:1730530/1‑93 (MQ=255)
                                                           aCGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAata                              <  1:767641/93‑1 (MQ=255)
                                                           aCGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAata                              <  1:3609873/93‑1 (MQ=255)
                                                           aCGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAata                              >  1:1726037/1‑93 (MQ=255)
                                                             ggCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATg                            <  1:3661381/93‑1 (MQ=255)
                                                             ggCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATg                            <  1:4959798/93‑1 (MQ=255)
                                                              gcacgcacCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATgg                           <  2:5721894/93‑1 (MQ=255)
                                                              gcacgcacCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATgg                           <  1:946725/93‑1 (MQ=255)
                                                              gcacgcacCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATgg                           <  1:2853832/93‑1 (MQ=255)
                                                              gcacgcacCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATgg                           >  2:5472817/1‑93 (MQ=255)
                                                               cacgcacCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGc                          <  2:3707253/93‑1 (MQ=255)
                                                                  gcacCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCggg                       <  2:3882413/93‑1 (MQ=255)
                                                                  gcacCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCggg                       <  1:4613043/93‑1 (MQ=255)
                                                                  gcacCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCggg                       >  2:2979062/1‑93 (MQ=255)
                                                                  gcacCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCggg                       >  1:894779/1‑93 (MQ=255)
                                                                   cacCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCgggg                      >  1:5961091/1‑92 (MQ=255)
                                                                   cacCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGt                      <  2:4272135/93‑1 (MQ=255)
                                                                    acCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCggggg                     >  2:732204/1‑91 (MQ=255)
                                                                    acCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCggggg                     >  2:5182100/1‑91 (MQ=255)
                                                                     ccGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCgggggg                    >  2:3107074/1‑90 (MQ=255)
                                                                      cGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCt                   <  1:1855359/93‑1 (MQ=255)
                                                                        tCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGGGCTCg                 >  2:2822024/1‑93 (MQ=255)
                                                                         cccATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGt                <  1:3547857/93‑1 (MQ=255)
                                                                         cccATTGACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGt                <  2:1128666/93‑1 (MQ=255)
                                                                          ccATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGGGGTCGTg               >  2:5030884/1‑93 (MQ=255)
                                                                          ccATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGGGCTCGTg               >  2:4770930/1‑93 (MQ=255)
                                                                             tttACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTgcgc            <  1:5830924/93‑1 (MQ=255)
                                                                             tttACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGGGGTCgcgcgc            >  1:209485/1‑93 (MQ=255)
                                                                                  gagaCAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTgg       <  1:2934746/93‑1 (MQ=255)
                                                                                  gagaCAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTgg       <  2:4568766/93‑1 (MQ=255)
                                                                                   agacagatACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGGGGTCGTGTGCGGTggg      >  1:4421269/1‑93 (MQ=255)
                                                                                       agataCTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGGGGTCGTGCGCTGTggggcgc  >  2:1465319/1‑89 (MQ=255)
                                                                                          |                                                                                         
GAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGGCCCA  >  NC_000913/3950992‑3951171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGGCCCACCG  >  NC_000913/3950989‑3951174
                                                                                             |                                                                                            
GGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGAT                                                                                              <  SRR4302533.428302/94‑1 (MQ=60)
GGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGAT                                                                                              <  SRR4302533.3976472/94‑1 (MQ=60)
 GCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATA                                                                                             <  SRR4302533.1961565/94‑1 (MQ=60)
 GCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATA                                                                                             >  SRR4302533.2324940/1‑94 (MQ=60)
  GGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATAC                                                                                            <  SRR4302533.882928/94‑1 (MQ=60)
  CGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATAC                                                                                            >  SRR4302533.1682591/1‑94 (MQ=60)
  CGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATAC                                                                                            <  SRR4302533.2247694/94‑1 (MQ=60)
   GAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACT                                                                                           <  SRR4302533.1711539/94‑1 (MQ=60)
   GAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACT                                                                                           >  SRR4302533.5313157/1‑94 (MQ=60)
   GAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACT                                                                                           >  SRR4302533.3926854/1‑94 (MQ=60)
    AAACCGAAGATAAATGGGGCTGGTTAGATCATGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTG                                                                                          <  SRR4302533.2115648/94‑1 (MQ=60)
    AAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTG                                                                                          <  SRR4302533.3047021/94‑1 (MQ=60)
    AAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTG                                                                                          <  SRR4302533.36205/94‑1 (MQ=60)
    AAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTG                                                                                          >  SRR4302533.2344638/1‑94 (MQ=60)
    AAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTG                                                                                          >  SRR4302533.2412267/1‑94 (MQ=60)
     AGCCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGG                                                                                         <  SRR4302533.28980/94‑1 (MQ=60)
     AACCGAAGATAAATGGGGCTGGTTAGATCGAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTTG                                                                                         <  SRR4302533.5979296/94‑1 (MQ=60)
     AACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGAACGCACCGTCCCATTTACGAGACAGATACTGG                                                                                         >  SRR4302533.5031091/1‑94 (MQ=60)
      ACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGG                                                                                        <  SRR4302533.3428931/94‑1 (MQ=60)
      ACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGG                                                                                        <  SRR4302533.2045862/94‑1 (MQ=60)
       CCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGA                                                                                       >  SRR4302533.1037455/1‑94 (MQ=60)
       CCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGA                                                                                       <  SRR4302533.2633146/94‑1 (MQ=60)
       CCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGA                                                                                       <  SRR4302533.5161231/94‑1 (MQ=60)
        CGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAG                                                                                      <  SRR4302533.2835944/94‑1 (MQ=60)
        CGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAG                                                                                      <  SRR4302533.1403290/94‑1 (MQ=60)
        CGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAG                                                                                      >  SRR4302533.5300587/1‑94 (MQ=60)
        CGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAG                                                                                      <  SRR4302533.3830610/94‑1 (MQ=60)
         GAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGT                                                                                     >  SRR4302533.3743480/1‑94 (MQ=60)
         GAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGT                                                                                     <  SRR4302533.5695106/94‑1 (MQ=60)
         GAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGT                                                                                     >  SRR4302533.3523749/1‑94 (MQ=60)
         GAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGT                                                                                     <  SRR4302533.4046451/94‑1 (MQ=60)
         GAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGT                                                                                     <  SRR4302533.1578796/94‑1 (MQ=60)
          AAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTA                                                                                    <  SRR4302533.4568583/94‑1 (MQ=60)
           AGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAA                                                                                   <  SRR4302533.152374/94‑1 (MQ=60)
           AGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAA                                                                                   >  SRR4302533.2806397/1‑94 (MQ=60)
           AGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAA                                                                                   >  SRR4302533.127198/1‑94 (MQ=60)
           AGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAA                                                                                   <  SRR4302533.3049741/94‑1 (MQ=60)
             ATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAAT                                                                                 <  SRR4302533.142985/94‑1 (MQ=60)
              TAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATA                                                                                <  SRR4302533.2393626/94‑1 (MQ=60)
               AAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGGACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAA                                                                               >  SRR4302533.94295/1‑94 (MQ=60)
               AAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAA                                                                               <  SRR4302533.5376837/94‑1 (MQ=60)
               AAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAA                                                                               >  SRR4302533.4110718/1‑94 (MQ=60)
               AAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAA                                                                               >  SRR4302533.2392025/1‑94 (MQ=60)
               AAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAA                                                                               <  SRR4302533.4803192/94‑1 (MQ=60)
                AATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAA                                                                              >  SRR4302533.3982970/1‑94 (MQ=60)
                 ATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAG                                                                             >  SRR4302533.1047770/1‑94 (MQ=60)
                 ATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAG                                                                             <  SRR4302533.4738524/94‑1 (MQ=60)
                 ATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAG                                                                             >  SRR4302533.1137858/1‑94 (MQ=60)
                   GGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTA                                                                           >  SRR4302533.735073/1‑94 (MQ=60)
                    GGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTAT                                                                          <  SRR4302533.2272568/94‑1 (MQ=60)
                    GGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTAT                                                                          <  SRR4302533.692478/94‑1 (MQ=60)
                    GGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATGTACGAGACAGATACTGGGAGTAAATAAAGTAT                                                                          <  SRR4302533.3703183/94‑1 (MQ=60)
                    GGGCTGGTGAGATCAAGTTAATCAATAAATACAAAAAAGGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTAT                                                                          <  SRR4302533.6270124/94‑1 (MQ=60)
                     GGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATG                                                                         <  SRR4302533.5520421/94‑1 (MQ=60)
                        TGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCT                                                                      >  SRR4302533.5545805/1‑94 (MQ=60)
                        TGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCT                                                                      <  SRR4302533.2191385/94‑1 (MQ=60)
                         GGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTA                                                                     >  SRR4302533.4107213/1‑94 (MQ=60)
                         GGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTA                                                                     >  SRR4302533.1280823/1‑94 (MQ=60)
                          GTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGCTACTGGGAGTAAATAAAGTATGCCTAA                                                                    >  SRR4302533.1422528/1‑94 (MQ=60)
                          GTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAA                                                                    >  SRR4302533.1735305/1‑94 (MQ=60)
                          GTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAA                                                                    >  SRR4302533.3906242/1‑94 (MQ=60)
                           TTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAG                                                                   <  SRR4302533.5424795/94‑1 (MQ=60)
                           TTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAG                                                                   <  SRR4302533.4467948/94‑1 (MQ=60)
                           TTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAG                                                                   >  SRR4302533.4050834/1‑94 (MQ=60)
                            TAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGT                                                                  <  SRR4302533.4102250/94‑1 (MQ=60)
                             AGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTA                                                                 <  SRR4302533.881915/94‑1 (MQ=60)
                             AGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTA                                                                 >  SRR4302533.2224800/1‑94 (MQ=60)
                              GATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTAC                                                                >  SRR4302533.2180357/1‑94 (MQ=60)
                              GATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTAC                                                                <  SRR4302533.409373/94‑1 (MQ=60)
                              GATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTAC                                                                >  SRR4302533.4545117/1‑94 (MQ=60)
                               ATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACC                                                               <  SRR4302533.3518001/94‑1 (MQ=60)
                               ATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACC                                                               >  SRR4302533.835846/1‑94 (MQ=60)
                                TCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCG                                                              >  SRR4302533.5824886/1‑94 (MQ=60)
                                TCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCG                                                              >  SRR4302533.5824610/1‑94 (MQ=60)
                                TCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCG                                                              <  SRR4302533.4358043/94‑1 (MQ=60)
                                 CAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGT                                                             >  SRR4302533.3257182/1‑94 (MQ=60)
                                 CAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGT                                                             >  SRR4302533.5869120/1‑94 (MQ=60)
                                 CAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGT                                                             <  SRR4302533.2895753/94‑1 (MQ=60)
                                 CAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGACCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGT                                                             >  SRR4302533.3060020/1‑94 (MQ=60)
                                  AAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTT                                                            <  SRR4302533.3638432/94‑1 (MQ=60)
                                  AAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTT                                                            <  SRR4302533.5613075/94‑1 (MQ=60)
                                   AGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATGCTGGGAGTAAATAAAGTATGCCTAAGTACCGTTC                                                           <  SRR4302533.5745464/94‑1 (MQ=60)
                                   AGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTC                                                           <  SRR4302533.3072143/94‑1 (MQ=60)
                                   AGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTC                                                           >  SRR4302533.1366517/1‑94 (MQ=60)
                                    GTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCTTTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCC                                                          >  SRR4302533.4465037/1‑94 (MQ=60)
                                    GTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTGCGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCC                                                          <  SRR4302533.4111886/94‑1 (MQ=60)
                                    GTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCG                                                          >  SRR4302533.5695141/1‑94 (MQ=60)
                                    GTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCC                                                          <  SRR4302533.5532011/94‑1 (MQ=60)
                                      TAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGC                                                        >  SRR4302533.4088747/1‑94 (MQ=60)
                                        ATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCA                                                      >  SRR4302533.1279444/1‑94 (MQ=60)
                                         TCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCAC                                                     >  SRR4302533.4382080/1‑94 (MQ=60)
                                          CAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACC                                                    >  SRR4302533.387393/1‑94 (MQ=60)
                                           AATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCA                                                   >  SRR4302533.1723844/1‑94 (MQ=60)
                                            ATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCAC                                                  >  SRR4302533.2534124/1‑94 (MQ=60)
                                            ATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCAC                                                  >  SRR4302533.3848306/1‑94 (MQ=60)
                                            ATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCAC                                                  >  SRR4302533.1418213/1‑94 (MQ=60)
                                              AAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCA                                                <  SRR4302533.2400572/94‑1 (MQ=60)
                                              AAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCA                                                <  SRR4302533.6080427/94‑1 (MQ=60)
                                                ATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGCGCAAATAAAGTATGCCTAAGTACCGATCCGCCACCACCACA                                              >  SRR4302533.5608049/1‑94 (MQ=60)
                                                ATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACT                                              <  SRR4302533.4068376/94‑1 (MQ=60)
                                                  ACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCA                                            <  SRR4302533.1664187/94‑1 (MQ=60)
                                                   CAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCAC                                           >  SRR4302533.6085410/1‑94 (MQ=60)
                                                     AAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGG                                         <  SRR4302533.1250996/94‑1 (MQ=60)
                                                      AAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGT                                        >  SRR4302533.206048/1‑94 (MQ=60)
                                                       AAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTC                                       <  SRR4302533.738763/94‑1 (MQ=60)
                                                       AAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTC                                       >  SRR4302533.3384675/1‑94 (MQ=60)
                                                       AAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTC                                       <  SRR4302533.5381451/94‑1 (MQ=60)
                                                       AAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTC                                       >  SRR4302533.86434/1‑94 (MQ=60)
                                                       AAATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTC                                       <  SRR4302533.905134/94‑1 (MQ=60)
                                                        AATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCG                                      <  SRR4302533.2107493/94‑1 (MQ=60)
                                                        AATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCG                                      >  SRR4302533.1870758/1‑94 (MQ=60)
                                                         ATGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGA                                     >  SRR4302533.1964489/1‑94 (MQ=60)
                                                          TGGGACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTA                                    <  SRR4302533.2379475/94‑1 (MQ=60)
                                                            GGGCGGCACGCACCGTCCCAGTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAAT                                  <  SRR4302533.1068838/94‑1 (MQ=60)
                                                             GACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATA                                 >  SRR4302533.4423120/1‑94 (MQ=60)
                                                             GACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATA                                 >  SRR4302533.1736510/1‑94 (MQ=60)
                                                              ACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATAT                                <  SRR4302533.3632092/94‑1 (MQ=60)
                                                              ACGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATAT                                <  SRR4302533.772170/94‑1 (MQ=60)
                                                               CGGCACGCACCGTCCCATTTACGAGACAGATGCTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATG                               <  SRR4302533.1068898/94‑1 (MQ=60)
                                                               CGGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATG                               >  SRR4302533.1336072/1‑94 (MQ=60)
                                                                GGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGG                              <  SRR4302533.4989845/94‑1 (MQ=60)
                                                                GGCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGG                              <  SRR4302533.3683893/94‑1 (MQ=60)
                                                                 GCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGC                             <  SRR4302533.952289/94‑1 (MQ=60)
                                                                 GCACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGC                             <  SRR4302533.2871389/94‑1 (MQ=60)
                                                                   ACGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGG                           >  SRR4302533.4794483/1‑94 (MQ=60)
                                                                    CGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGG                          >  SRR4302533.900037/1‑94 (MQ=60)
                                                                    CGCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGG                          >  SRR4302533.4985742/1‑94 (MQ=60)
                                                                     GCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGT                         <  SRR4302533.5028315/94‑1 (MQ=60)
                                                                     GCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGT                         <  SRR4302533.4640988/94‑1 (MQ=60)
                                                                     GCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGG                         >  SRR4302533.5997154/1‑94 (MQ=60)
                                                                     GCACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGG                         >  SRR4302533.6097655/1‑94 (MQ=60)
                                                                      CACCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTG                        <  SRR4302533.4274398/94‑1 (MQ=60)
                                                                        CCGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGGGCT                      >  SRR4302533.6029687/1‑94 (MQ=60)
                                                                         CGTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTC                     <  SRR4302533.1866674/94‑1 (MQ=60)
                                                                          GTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGggggcg                    >  SRR4302533.3938025/1‑88 (MQ=60)
                                                                          GTCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGgggcgg                    >  SRR4302533.2977011/1‑88 (MQ=60)
                                                                           TCCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGGGCGCGT                   >  SRR4302533.1767526/1‑94 (MQ=60)
                                                                            CCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGgggtctgg                  >  SRR4302533.1032799/1‑86 (MQ=60)
                                                                            CCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGGTCGTG                  >  SRR4302533.850780/1‑94 (MQ=60)
                                                                            CCCATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTG                  <  SRR4302533.3569722/94‑1 (MQ=60)
                                                                              CATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCCCCACTCATGGTCGTAATATGGCGGGTGCTCGTGCG                <  SRR4302533.3759481/94‑1 (MQ=60)
                                                                              CATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGggggcctggc                >  SRR4302533.3579606/1‑84 (MQ=60)
                                                                               ATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGC               <  SRR4302533.1893327/94‑1 (MQ=60)
                                                                               ATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGGGGTCGTGCGC               >  SRR4302533.3608255/1‑94 (MQ=60)
                                                                               ATTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGGGGTCGCGCGC               >  SRR4302533.210809/1‑94 (MQ=60)
                                                                                TTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCT              <  SRR4302533.3819921/94‑1 (MQ=60)
                                                                                TTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCT              <  SRR4302533.745226/94‑1 (MQ=60)
                                                                                TTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCT              <  SRR4302533.3658748/94‑1 (MQ=60)
                                                                                TTTACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCT              <  SRR4302533.5866167/94‑1 (MQ=60)
                                                                                   ACGAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTG           <  SRR4302533.1840555/94‑1 (MQ=60)
                                                                                     GAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGC         <  SRR4302533.2952690/94‑1 (MQ=60)
                                                                                     GAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGGGGTCGTGTGCGGTGGG         >  SRR4302533.4447999/1‑94 (MQ=60)
                                                                                     GAGACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGGGGTCGTGCGCCGTGGG         >  SRR4302533.1656891/1‑94 (MQ=60)
                                                                                     GAGACAGATACTGGGAGTAAAGAAAGTATGCCTAAGTACCGTTCCGCCACCACCTCTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGC         <  SRR4302533.2101158/94‑1 (MQ=60)
                                                                                       GACAGATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGcgc       <  SRR4302533.1506879/94‑4 (MQ=60)
                                                                                            ATACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGcgcgccac  <  SRR4302533.2842705/94‑9 (MQ=60)
                                                                                             |                                                                                            
GGCGAAACCGAAGATAAATGGGGCTGGTTAGATCAAGTTAATCAATAAATACAAAAAATGGGACGGCACGCACCGTCCCATTTACGAGACAGACACTGGGAGTAAATAAAGTATGCCTAAGTACCGTTCCGCCACCACCACTCATGGTCGTAATATGGCGGGTGCTCGTGCGCTGTGGGCCCACCG  >  NC_000913/3950989‑3951174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑